Halobacteriovorax sp. DA5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Oligoflexia; Bacteriovoracales; Halobacteriovoraceae; Halobacteriovorax; unclassified Halobacteriovorax

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S3QRH4|A0A2S3QRH4_9PROT Polyamine aminopropyltransferase OS=Halobacteriovorax sp. DA5 OX=2067553 GN=speE PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 10.49KK3 pKa = 10.13LLAVAALGTLLVSCGGSGGSSSSSTYY29 pKa = 10.54GRR31 pKa = 11.84YY32 pKa = 7.35TSPYY36 pKa = 7.82VTAQGFVSALNSVDD50 pKa = 3.32PYY52 pKa = 11.5YY53 pKa = 11.31YY54 pKa = 10.75DD55 pKa = 5.46LNTLEE60 pKa = 4.28KK61 pKa = 10.6DD62 pKa = 3.33QYY64 pKa = 11.69DD65 pKa = 3.81SLRR68 pKa = 11.84EE69 pKa = 3.89DD70 pKa = 3.41FFVYY74 pKa = 10.2FDD76 pKa = 3.3AKK78 pKa = 10.39YY79 pKa = 10.21NEE81 pKa = 4.35FVAVDD86 pKa = 3.23ITYY89 pKa = 10.63LRR91 pKa = 11.84TLAYY95 pKa = 8.68WDD97 pKa = 4.36YY98 pKa = 11.19YY99 pKa = 11.56SNNQGLADD107 pKa = 4.31EE108 pKa = 5.02FRR110 pKa = 11.84DD111 pKa = 3.99IQSDD115 pKa = 3.89DD116 pKa = 3.0AWDD119 pKa = 3.5YY120 pKa = 11.81GIIGDD125 pKa = 3.98GWGDD129 pKa = 3.14NYY131 pKa = 10.86EE132 pKa = 3.94IVDD135 pKa = 3.93YY136 pKa = 11.27VGSDD140 pKa = 3.4VFGDD144 pKa = 3.71PVFQGFDD151 pKa = 2.56TGYY154 pKa = 9.13YY155 pKa = 9.82YY156 pKa = 10.56EE157 pKa = 5.49DD158 pKa = 3.95EE159 pKa = 4.76EE160 pKa = 4.49EE161 pKa = 4.33TMDD164 pKa = 3.95TGLMAANDD172 pKa = 3.95EE173 pKa = 4.15EE174 pKa = 4.69LQMFNKK180 pKa = 10.28ASLYY184 pKa = 9.94SQAFKK189 pKa = 11.19LPADD193 pKa = 3.79KK194 pKa = 10.85ALSLVTMEE202 pKa = 4.8KK203 pKa = 10.14EE204 pKa = 4.31VKK206 pKa = 10.48KK207 pKa = 10.44MLDD210 pKa = 3.27NAKK213 pKa = 10.55DD214 pKa = 3.84GEE216 pKa = 4.5LLEE219 pKa = 4.61QDD221 pKa = 3.5QQAIANNIKK230 pKa = 10.33HH231 pKa = 5.35FTGKK235 pKa = 10.37SIEE238 pKa = 4.1EE239 pKa = 3.68FMAAKK244 pKa = 10.26NDD246 pKa = 3.53LNARR250 pKa = 11.84EE251 pKa = 4.01QIIDD255 pKa = 3.95DD256 pKa = 4.04VAGHH260 pKa = 7.5LGTTTQNIEE269 pKa = 4.2DD270 pKa = 4.11VILPEE275 pKa = 4.48LLSIEE280 pKa = 4.23LL281 pKa = 3.87

Molecular weight: 31.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S3QNG6|A0A2S3QNG6_9PROT Uncharacterized protein OS=Halobacteriovorax sp. DA5 OX=2067553 GN=C0Z22_12180 PE=4 SV=1
MM1 pKa = 7.83SKK3 pKa = 9.05RR4 pKa = 11.84TWQPKK9 pKa = 7.5RR10 pKa = 11.84KK11 pKa = 9.76KK12 pKa = 10.09RR13 pKa = 11.84MRR15 pKa = 11.84VHH17 pKa = 6.7GFLKK21 pKa = 10.53RR22 pKa = 11.84MEE24 pKa = 4.33TAGGRR29 pKa = 11.84NVIKK33 pKa = 10.5ARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 8.23QLTVSHH47 pKa = 6.53GKK49 pKa = 9.68KK50 pKa = 10.19

Molecular weight: 5.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3125

0

3125

1005824

37

2539

321.9

36.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.392 ± 0.045

1.066 ± 0.017

5.813 ± 0.034

7.025 ± 0.043

5.128 ± 0.044

6.225 ± 0.055

1.884 ± 0.018

7.615 ± 0.052

7.997 ± 0.056

9.524 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.307 ± 0.021

5.468 ± 0.035

3.1 ± 0.023

3.252 ± 0.026

3.977 ± 0.024

7.143 ± 0.042

5.275 ± 0.049

6.151 ± 0.036

0.843 ± 0.013

3.816 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski