Amino acid dipepetide frequency for Streptomyces sp. (strain SirexAA-E / ActE)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.257AlaAla: 21.257 ± 0.168	0.999AlaCys: 0.999 ± 0.022	8.512AlaAsp: 8.512 ± 0.066	8.834AlaGlu: 8.834 ± 0.092	3.532AlaPhe: 3.532 ± 0.047	13.458AlaGly: 13.458 ± 0.092	2.846AlaHis: 2.846 ± 0.037	3.024AlaIle: 3.024 ± 0.047	2.706AlaLys: 2.706 ± 0.055	14.697AlaLeu: 14.697 ± 0.121	2.525AlaMet: 2.525 ± 0.037	1.754AlaAsn: 1.754 ± 0.032	7.639AlaPro: 7.639 ± 0.09	3.687AlaGln: 3.687 ± 0.049	10.431AlaArg: 10.431 ± 0.085	6.133AlaSer: 6.133 ± 0.057	7.049AlaThr: 7.049 ± 0.063	12.925AlaVal: 12.925 ± 0.111	1.808AlaTrp: 1.808 ± 0.031	2.794AlaTyr: 2.794 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.059CysAla: 1.059 ± 0.026	0.086CysCys: 0.086 ± 0.008	0.464CysAsp: 0.464 ± 0.014	0.376CysGlu: 0.376 ± 0.014	0.212CysPhe: 0.212 ± 0.011	0.957CysGly: 0.957 ± 0.022	0.181CysHis: 0.181 ± 0.009	0.138CysIle: 0.138 ± 0.009	0.102CysLys: 0.102 ± 0.008	0.689CysLeu: 0.689 ± 0.019	0.126CysMet: 0.126 ± 0.008	0.11CysAsn: 0.11 ± 0.007	0.46CysPro: 0.46 ± 0.014	0.14CysGln: 0.14 ± 0.008	0.59CysArg: 0.59 ± 0.018	0.419CysSer: 0.419 ± 0.017	0.492CysThr: 0.492 ± 0.017	0.661CysVal: 0.661 ± 0.017	0.118CysTrp: 0.118 ± 0.008	0.142CysTyr: 0.142 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.081AspAla: 8.081 ± 0.068	0.406AspCys: 0.406 ± 0.017	3.691AspAsp: 3.691 ± 0.051	3.949AspGlu: 3.949 ± 0.042	1.648AspPhe: 1.648 ± 0.032	6.691AspGly: 6.691 ± 0.074	1.429AspHis: 1.429 ± 0.027	1.855AspIle: 1.855 ± 0.029	1.165AspLys: 1.165 ± 0.033	6.062AspLeu: 6.062 ± 0.061	0.832AspMet: 0.832 ± 0.019	0.924AspAsn: 0.924 ± 0.02	4.577AspPro: 4.577 ± 0.058	1.472AspGln: 1.472 ± 0.029	5.126AspArg: 5.126 ± 0.054	2.559AspSer: 2.559 ± 0.039	3.462AspThr: 3.462 ± 0.047	4.777AspVal: 4.777 ± 0.049	0.975AspTrp: 0.975 ± 0.023	1.073AspTyr: 1.073 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.696GluAla: 7.696 ± 0.079	0.347GluCys: 0.347 ± 0.013	3.002GluAsp: 3.002 ± 0.042	3.742GluGlu: 3.742 ± 0.062	1.367GluPhe: 1.367 ± 0.024	4.539GluGly: 4.539 ± 0.051	1.452GluHis: 1.452 ± 0.029	2.232GluIle: 2.232 ± 0.04	1.486GluLys: 1.486 ± 0.034	6.667GluLeu: 6.667 ± 0.061	0.9GluMet: 0.9 ± 0.022	1.052GluAsn: 1.052 ± 0.022	3.437GluPro: 3.437 ± 0.049	2.231GluGln: 2.231 ± 0.046	5.437GluArg: 5.437 ± 0.054	2.601GluSer: 2.601 ± 0.039	2.926GluThr: 2.926 ± 0.033	4.398GluVal: 4.398 ± 0.053	0.722GluTrp: 0.722 ± 0.02	1.051GluTyr: 1.051 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.647PheAla: 3.647 ± 0.041	0.272PheCys: 0.272 ± 0.012	1.968PheAsp: 1.968 ± 0.03	1.391PheGlu: 1.391 ± 0.031	0.877PhePhe: 0.877 ± 0.022	3.05PheGly: 3.05 ± 0.04	0.613PheHis: 0.613 ± 0.018	0.661PheIle: 0.661 ± 0.018	0.476PheLys: 0.476 ± 0.015	2.596PheLeu: 2.596 ± 0.039	0.399PheMet: 0.399 ± 0.013	0.538PheAsn: 0.538 ± 0.016	1.368PhePro: 1.368 ± 0.028	0.657PheGln: 0.657 ± 0.017	1.834PheArg: 1.834 ± 0.032	1.452PheSer: 1.452 ± 0.027	2.007PheThr: 2.007 ± 0.035	2.289PheVal: 2.289 ± 0.038	0.413PheTrp: 0.413 ± 0.013	0.569PheTyr: 0.569 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.687GlyAla: 11.687 ± 0.088	0.818GlyCys: 0.818 ± 0.023	5.28GlyAsp: 5.28 ± 0.059	5.225GlyGlu: 5.225 ± 0.047	2.891GlyPhe: 2.891 ± 0.039	9.154GlyGly: 9.154 ± 0.084	2.339GlyHis: 2.339 ± 0.031	3.39GlyIle: 3.39 ± 0.047	2.388GlyLys: 2.388 ± 0.04	9.642GlyLeu: 9.642 ± 0.077	1.986GlyMet: 1.986 ± 0.036	1.714GlyAsn: 1.714 ± 0.032	5.653GlyPro: 5.653 ± 0.068	2.614GlyGln: 2.614 ± 0.039	8.218GlyArg: 8.218 ± 0.074	5.506GlySer: 5.506 ± 0.057	6.669GlyThr: 6.669 ± 0.063	7.619GlyVal: 7.619 ± 0.072	1.665GlyTrp: 1.665 ± 0.031	2.288GlyTyr: 2.288 ± 0.036	0.0GlyXaa: 0.0 ± 0.0
His	2.644HisAla: 2.644 ± 0.037	0.195HisCys: 0.195 ± 0.009	1.321HisAsp: 1.321 ± 0.025	1.186HisGlu: 1.186 ± 0.022	0.631HisPhe: 0.631 ± 0.018	2.382HisGly: 2.382 ± 0.032	0.698HisHis: 0.698 ± 0.022	0.675HisIle: 0.675 ± 0.018	0.318HisLys: 0.318 ± 0.013	2.438HisLeu: 2.438 ± 0.037	0.333HisMet: 0.333 ± 0.013	0.364HisAsn: 0.364 ± 0.013	1.796HisPro: 1.796 ± 0.031	0.638HisGln: 0.638 ± 0.019	2.152HisArg: 2.152 ± 0.032	0.991HisSer: 0.991 ± 0.022	1.366HisThr: 1.366 ± 0.028	1.641HisVal: 1.641 ± 0.032	0.355HisTrp: 0.355 ± 0.013	0.447HisTyr: 0.447 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.543IleAla: 4.543 ± 0.048	0.289IleCys: 0.289 ± 0.012	2.174IleAsp: 2.174 ± 0.032	1.866IleGlu: 1.866 ± 0.035	0.666IlePhe: 0.666 ± 0.019	3.545IleGly: 3.545 ± 0.046	0.562IleHis: 0.562 ± 0.017	0.816IleIle: 0.816 ± 0.025	0.68IleLys: 0.68 ± 0.021	2.269IleLeu: 2.269 ± 0.039	0.419IleMet: 0.419 ± 0.014	0.639IleAsn: 0.639 ± 0.019	1.673IlePro: 1.673 ± 0.028	0.687IleGln: 0.687 ± 0.017	2.092IleArg: 2.092 ± 0.036	1.572IleSer: 1.572 ± 0.029	2.04IleThr: 2.04 ± 0.035	2.546IleVal: 2.546 ± 0.036	0.349IleTrp: 0.349 ± 0.014	0.483IleTyr: 0.483 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	2.872LysAla: 2.872 ± 0.051	0.09LysCys: 0.09 ± 0.006	1.365LysAsp: 1.365 ± 0.031	1.212LysGlu: 1.212 ± 0.03	0.435LysPhe: 0.435 ± 0.016	1.821LysGly: 1.821 ± 0.038	0.389LysHis: 0.389 ± 0.014	0.848LysIle: 0.848 ± 0.025	0.897LysLys: 0.897 ± 0.031	1.916LysLeu: 1.916 ± 0.034	0.383LysMet: 0.383 ± 0.016	0.514LysAsn: 0.514 ± 0.019	1.266LysPro: 1.266 ± 0.033	0.694LysGln: 0.694 ± 0.025	1.361LysArg: 1.361 ± 0.03	1.087LysSer: 1.087 ± 0.026	1.206LysThr: 1.206 ± 0.029	1.826LysVal: 1.826 ± 0.037	0.252LysTrp: 0.252 ± 0.012	0.418LysTyr: 0.418 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	14.821LeuAla: 14.821 ± 0.116	0.824LeuCys: 0.824 ± 0.019	6.798LeuAsp: 6.798 ± 0.06	4.695LeuGlu: 4.695 ± 0.061	2.636LeuPhe: 2.636 ± 0.039	9.41LeuGly: 9.41 ± 0.083	2.29LeuHis: 2.29 ± 0.031	3.094LeuIle: 3.094 ± 0.041	1.876LeuLys: 1.876 ± 0.036	11.378LeuLeu: 11.378 ± 0.116	1.711LeuMet: 1.711 ± 0.033	1.598LeuAsn: 1.598 ± 0.028	6.533LeuPro: 6.533 ± 0.073	2.091LeuGln: 2.091 ± 0.036	8.652LeuArg: 8.652 ± 0.077	5.375LeuSer: 5.375 ± 0.053	6.948LeuThr: 6.948 ± 0.062	9.094LeuVal: 9.094 ± 0.086	1.252LeuTrp: 1.252 ± 0.026	1.789LeuTyr: 1.789 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.278MetAla: 2.278 ± 0.035	0.13MetCys: 0.13 ± 0.008	0.884MetAsp: 0.884 ± 0.022	0.788MetGlu: 0.788 ± 0.019	0.458MetPhe: 0.458 ± 0.016	1.318MetGly: 1.318 ± 0.028	0.347MetHis: 0.347 ± 0.013	0.638MetIle: 0.638 ± 0.018	0.411MetLys: 0.411 ± 0.015	1.669MetLeu: 1.669 ± 0.029	0.31MetMet: 0.31 ± 0.012	0.434MetAsn: 0.434 ± 0.015	1.096MetPro: 1.096 ± 0.025	0.442MetGln: 0.442 ± 0.015	1.454MetArg: 1.454 ± 0.03	1.326MetSer: 1.326 ± 0.025	1.551MetThr: 1.551 ± 0.027	1.29MetVal: 1.29 ± 0.023	0.202MetTrp: 0.202 ± 0.009	0.325MetTyr: 0.325 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.065AsnAla: 2.065 ± 0.036	0.147AsnCys: 0.147 ± 0.009	0.966AsnAsp: 0.966 ± 0.022	0.791AsnGlu: 0.791 ± 0.021	0.46AsnPhe: 0.46 ± 0.015	1.852AsnGly: 1.852 ± 0.036	0.372AsnHis: 0.372 ± 0.014	0.612AsnIle: 0.612 ± 0.018	0.385AsnLys: 0.385 ± 0.017	1.585AsnLeu: 1.585 ± 0.029	0.287AsnMet: 0.287 ± 0.011	0.389AsnAsn: 0.389 ± 0.014	1.241AsnPro: 1.241 ± 0.024	0.463AsnGln: 0.463 ± 0.019	1.255AsnArg: 1.255 ± 0.026	0.883AsnSer: 0.883 ± 0.023	1.073AsnThr: 1.073 ± 0.026	1.301AsnVal: 1.301 ± 0.031	0.266AsnTrp: 0.266 ± 0.013	0.374AsnTyr: 0.374 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.048ProAla: 9.048 ± 0.09	0.328ProCys: 0.328 ± 0.013	4.698ProAsp: 4.698 ± 0.057	4.435ProGlu: 4.435 ± 0.053	1.551ProPhe: 1.551 ± 0.024	7.046ProGly: 7.046 ± 0.072	1.367ProHis: 1.367 ± 0.025	1.073ProIle: 1.073 ± 0.023	1.108ProLys: 1.108 ± 0.025	5.407ProLeu: 5.407 ± 0.055	0.996ProMet: 0.996 ± 0.021	0.787ProAsn: 0.787 ± 0.02	3.614ProPro: 3.614 ± 0.069	1.798ProGln: 1.798 ± 0.035	4.03ProArg: 4.03 ± 0.047	3.304ProSer: 3.304 ± 0.039	3.069ProThr: 3.069 ± 0.042	5.733ProVal: 5.733 ± 0.062	0.908ProTrp: 0.908 ± 0.023	1.414ProTyr: 1.414 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.663GlnAla: 3.663 ± 0.045	0.147GlnCys: 0.147 ± 0.009	1.548GlnAsp: 1.548 ± 0.035	1.522GlnGlu: 1.522 ± 0.035	0.649GlnPhe: 0.649 ± 0.018	2.333GlnGly: 2.333 ± 0.036	0.601GlnHis: 0.601 ± 0.018	1.05GlnIle: 1.05 ± 0.022	0.625GlnLys: 0.625 ± 0.021	2.935GlnLeu: 2.935 ± 0.044	0.472GlnMet: 0.472 ± 0.014	0.505GlnAsn: 0.505 ± 0.016	1.598GlnPro: 1.598 ± 0.036	1.185GlnGln: 1.185 ± 0.038	2.181GlnArg: 2.181 ± 0.038	1.19GlnSer: 1.19 ± 0.023	1.26GlnThr: 1.26 ± 0.027	2.337GlnVal: 2.337 ± 0.04	0.417GlnTrp: 0.417 ± 0.015	0.561GlnTyr: 0.561 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.946ArgAla: 9.946 ± 0.095	0.583ArgCys: 0.583 ± 0.019	4.223ArgAsp: 4.223 ± 0.045	4.712ArgGlu: 4.712 ± 0.05	2.306ArgPhe: 2.306 ± 0.033	6.015ArgGly: 6.015 ± 0.057	2.119ArgHis: 2.119 ± 0.035	3.217ArgIle: 3.217 ± 0.04	1.64ArgLys: 1.64 ± 0.032	8.687ArgLeu: 8.687 ± 0.084	1.756ArgMet: 1.756 ± 0.026	1.349ArgAsn: 1.349 ± 0.029	5.211ArgPro: 5.211 ± 0.058	2.214ArgGln: 2.214 ± 0.037	7.873ArgArg: 7.873 ± 0.083	4.245ArgSer: 4.245 ± 0.044	5.902ArgThr: 5.902 ± 0.053	5.837ArgVal: 5.837 ± 0.06	1.373ArgTrp: 1.373 ± 0.026	1.77ArgTyr: 1.77 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	6.808SerAla: 6.808 ± 0.057	0.39SerCys: 0.39 ± 0.013	2.739SerAsp: 2.739 ± 0.037	2.38SerGlu: 2.38 ± 0.035	1.557SerPhe: 1.557 ± 0.026	6.107SerGly: 6.107 ± 0.068	1.052SerHis: 1.052 ± 0.024	1.348SerIle: 1.348 ± 0.03	1.015SerLys: 1.015 ± 0.024	4.873SerLeu: 4.873 ± 0.051	1.049SerMet: 1.049 ± 0.024	0.828SerAsn: 0.828 ± 0.022	3.208SerPro: 3.208 ± 0.044	1.218SerGln: 1.218 ± 0.023	3.769SerArg: 3.769 ± 0.044	2.9SerSer: 2.9 ± 0.04	3.163SerThr: 3.163 ± 0.043	4.476SerVal: 4.476 ± 0.049	0.934SerTrp: 0.934 ± 0.022	1.214SerTyr: 1.214 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	9.209ThrAla: 9.209 ± 0.081	0.389ThrCys: 0.389 ± 0.014	3.795ThrAsp: 3.795 ± 0.048	3.412ThrGlu: 3.412 ± 0.043	1.626ThrPhe: 1.626 ± 0.031	7.152ThrGly: 7.152 ± 0.066	1.197ThrHis: 1.197 ± 0.022	1.484ThrIle: 1.484 ± 0.027	1.122ThrLys: 1.122 ± 0.03	5.825ThrLeu: 5.825 ± 0.058	0.92ThrMet: 0.92 ± 0.024	0.925ThrAsn: 0.925 ± 0.025	4.119ThrPro: 4.119 ± 0.052	1.345ThrGln: 1.345 ± 0.027	3.924ThrArg: 3.924 ± 0.043	3.192ThrSer: 3.192 ± 0.049	3.902ThrThr: 3.902 ± 0.061	6.333ThrVal: 6.333 ± 0.063	0.899ThrTrp: 0.899 ± 0.022	1.353ThrTyr: 1.353 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	10.838ValAla: 10.838 ± 0.088	0.766ValCys: 0.766 ± 0.021	5.054ValAsp: 5.054 ± 0.052	4.782ValGlu: 4.782 ± 0.057	2.473ValPhe: 2.473 ± 0.036	6.588ValGly: 6.588 ± 0.058	1.979ValHis: 1.979 ± 0.029	2.728ValIle: 2.728 ± 0.041	1.718ValLys: 1.718 ± 0.036	9.815ValLeu: 9.815 ± 0.097	1.43ValMet: 1.43 ± 0.026	1.58ValAsn: 1.58 ± 0.031	5.441ValPro: 5.441 ± 0.053	2.034ValGln: 2.034 ± 0.028	7.513ValArg: 7.513 ± 0.063	4.474ValSer: 4.474 ± 0.049	5.739ValThr: 5.739 ± 0.053	8.264ValVal: 8.264 ± 0.092	1.122ValTrp: 1.122 ± 0.022	1.598ValTyr: 1.598 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.63TrpAla: 1.63 ± 0.028	0.138TrpCys: 0.138 ± 0.008	0.809TrpAsp: 0.809 ± 0.021	0.746TrpGlu: 0.746 ± 0.019	0.49TrpPhe: 0.49 ± 0.016	1.032TrpGly: 1.032 ± 0.024	0.351TrpHis: 0.351 ± 0.014	0.542TrpIle: 0.542 ± 0.018	0.373TrpLys: 0.373 ± 0.015	1.662TrpLeu: 1.662 ± 0.025	0.288TrpMet: 0.288 ± 0.012	0.388TrpAsn: 0.388 ± 0.013	0.748TrpPro: 0.748 ± 0.02	0.601TrpGln: 0.601 ± 0.017	1.291TrpArg: 1.291 ± 0.027	0.918TrpSer: 0.918 ± 0.022	1.055TrpThr: 1.055 ± 0.027	0.976TrpVal: 0.976 ± 0.02	0.322TrpTrp: 0.322 ± 0.013	0.342TrpTyr: 0.342 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.819TyrAla: 2.819 ± 0.029	0.177TyrCys: 0.177 ± 0.009	1.465TyrAsp: 1.465 ± 0.039	1.24TyrGlu: 1.24 ± 0.025	0.614TyrPhe: 0.614 ± 0.019	2.297TyrGly: 2.297 ± 0.042	0.386TyrHis: 0.386 ± 0.013	0.482TyrIle: 0.482 ± 0.015	0.361TyrLys: 0.361 ± 0.017	2.051TyrLeu: 2.051 ± 0.033	0.229TyrMet: 0.229 ± 0.009	0.375TyrAsn: 0.375 ± 0.015	1.034TyrPro: 1.034 ± 0.024	0.568TyrGln: 0.568 ± 0.018	1.793TyrArg: 1.793 ± 0.032	0.901TyrSer: 0.901 ± 0.024	1.245TyrThr: 1.245 ± 0.033	1.613TyrVal: 1.613 ± 0.035	0.355TyrTrp: 0.355 ± 0.015	0.425TyrTyr: 0.425 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6354 proteins (2142609 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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