Amino acid dipepetide frequency for Halorubrum vacuolatum (Natronobacterium vacuolatum)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.835AlaAla: 13.835 ± 0.201	0.756AlaCys: 0.756 ± 0.031	10.048AlaAsp: 10.048 ± 0.123	9.158AlaGlu: 9.158 ± 0.119	3.935AlaPhe: 3.935 ± 0.074	9.818AlaGly: 9.818 ± 0.105	1.923AlaHis: 1.923 ± 0.05	5.597AlaIle: 5.597 ± 0.082	1.882AlaLys: 1.882 ± 0.056	9.798AlaLeu: 9.798 ± 0.139	1.959AlaMet: 1.959 ± 0.046	2.277AlaAsn: 2.277 ± 0.048	3.577AlaPro: 3.577 ± 0.06	1.815AlaGln: 1.815 ± 0.042	6.27AlaArg: 6.27 ± 0.095	5.069AlaSer: 5.069 ± 0.075	7.001AlaThr: 7.001 ± 0.111	10.483AlaVal: 10.483 ± 0.118	1.031AlaTrp: 1.031 ± 0.045	2.754AlaTyr: 2.754 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.643CysAla: 0.643 ± 0.023	0.1CysCys: 0.1 ± 0.011	0.521CysAsp: 0.521 ± 0.025	0.525CysGlu: 0.525 ± 0.024	0.216CysPhe: 0.216 ± 0.013	0.807CysGly: 0.807 ± 0.034	0.204CysHis: 0.204 ± 0.014	0.28CysIle: 0.28 ± 0.018	0.112CysLys: 0.112 ± 0.011	0.583CysLeu: 0.583 ± 0.023	0.091CysMet: 0.091 ± 0.01	0.189CysAsn: 0.189 ± 0.016	0.521CysPro: 0.521 ± 0.025	0.146CysGln: 0.146 ± 0.013	0.571CysArg: 0.571 ± 0.025	0.438CysSer: 0.438 ± 0.023	0.427CysThr: 0.427 ± 0.02	0.503CysVal: 0.503 ± 0.025	0.085CysTrp: 0.085 ± 0.009	0.201CysTyr: 0.201 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	10.085AspAla: 10.085 ± 0.13	0.592AspCys: 0.592 ± 0.029	7.982AspAsp: 7.982 ± 0.144	7.565AspGlu: 7.565 ± 0.111	1.86AspPhe: 1.86 ± 0.045	8.552AspGly: 8.552 ± 0.119	1.938AspHis: 1.938 ± 0.059	3.016AspIle: 3.016 ± 0.063	0.993AspLys: 0.993 ± 0.037	7.224AspLeu: 7.224 ± 0.105	1.121AspMet: 1.121 ± 0.03	1.222AspAsn: 1.222 ± 0.046	5.44AspPro: 5.44 ± 0.099	1.53AspGln: 1.53 ± 0.042	7.201AspArg: 7.201 ± 0.086	3.274AspSer: 3.274 ± 0.062	4.171AspThr: 4.171 ± 0.081	7.799AspVal: 7.799 ± 0.104	0.975AspTrp: 0.975 ± 0.034	1.61AspTyr: 1.61 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	9.423GluAla: 9.423 ± 0.122	0.462GluCys: 0.462 ± 0.023	5.65GluAsp: 5.65 ± 0.101	7.406GluGlu: 7.406 ± 0.135	2.937GluPhe: 2.937 ± 0.054	5.735GluGly: 5.735 ± 0.076	2.097GluHis: 2.097 ± 0.047	4.198GluIle: 4.198 ± 0.087	1.786GluLys: 1.786 ± 0.057	7.632GluLeu: 7.632 ± 0.111	1.715GluMet: 1.715 ± 0.041	2.245GluAsn: 2.245 ± 0.058	4.441GluPro: 4.441 ± 0.071	2.219GluGln: 2.219 ± 0.063	7.955GluArg: 7.955 ± 0.116	4.733GluSer: 4.733 ± 0.074	6.717GluThr: 6.717 ± 0.089	5.924GluVal: 5.924 ± 0.088	1.097GluTrp: 1.097 ± 0.037	2.577GluTyr: 2.577 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	3.526PheAla: 3.526 ± 0.062	0.277PheCys: 0.277 ± 0.017	3.138PheAsp: 3.138 ± 0.057	3.29PheGlu: 3.29 ± 0.061	1.039PhePhe: 1.039 ± 0.038	3.255PheGly: 3.255 ± 0.069	0.639PheHis: 0.639 ± 0.025	1.458PheIle: 1.458 ± 0.04	0.542PheLys: 0.542 ± 0.023	2.835PheLeu: 2.835 ± 0.068	0.479PheMet: 0.479 ± 0.024	0.718PheAsn: 0.718 ± 0.029	1.323PhePro: 1.323 ± 0.039	0.668PheGln: 0.668 ± 0.026	1.869PheArg: 1.869 ± 0.048	1.701PheSer: 1.701 ± 0.052	2.029PheThr: 2.029 ± 0.041	2.934PheVal: 2.934 ± 0.061	0.375PheTrp: 0.375 ± 0.022	0.889PheTyr: 0.889 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	7.987GlyAla: 7.987 ± 0.108	0.666GlyCys: 0.666 ± 0.025	6.757GlyAsp: 6.757 ± 0.106	7.156GlyGlu: 7.156 ± 0.103	3.257GlyPhe: 3.257 ± 0.06	7.292GlyGly: 7.292 ± 0.115	1.665GlyHis: 1.665 ± 0.046	4.743GlyIle: 4.743 ± 0.074	1.886GlyLys: 1.886 ± 0.047	7.078GlyLeu: 7.078 ± 0.097	1.79GlyMet: 1.79 ± 0.05	1.835GlyAsn: 1.835 ± 0.056	3.361GlyPro: 3.361 ± 0.068	1.642GlyGln: 1.642 ± 0.036	5.246GlyArg: 5.246 ± 0.072	5.174GlySer: 5.174 ± 0.087	5.921GlyThr: 5.921 ± 0.1	7.489GlyVal: 7.489 ± 0.098	1.17GlyTrp: 1.17 ± 0.037	2.622GlyTyr: 2.622 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	2.248HisAla: 2.248 ± 0.053	0.16HisCys: 0.16 ± 0.013	1.923HisAsp: 1.923 ± 0.054	1.793HisGlu: 1.793 ± 0.043	0.474HisPhe: 0.474 ± 0.022	2.044HisGly: 2.044 ± 0.045	0.562HisHis: 0.562 ± 0.028	0.762HisIle: 0.762 ± 0.029	0.358HisLys: 0.358 ± 0.021	1.713HisLeu: 1.713 ± 0.043	0.292HisMet: 0.292 ± 0.019	0.499HisAsn: 0.499 ± 0.024	1.287HisPro: 1.287 ± 0.034	0.439HisGln: 0.439 ± 0.024	1.44HisArg: 1.44 ± 0.039	0.801HisSer: 0.801 ± 0.028	1.205HisThr: 1.205 ± 0.039	1.875HisVal: 1.875 ± 0.046	0.209HisTrp: 0.209 ± 0.014	0.531HisTyr: 0.531 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.432IleAla: 5.432 ± 0.088	0.32IleCys: 0.32 ± 0.018	4.934IleAsp: 4.934 ± 0.081	5.128IleGlu: 5.128 ± 0.085	1.063IlePhe: 1.063 ± 0.035	4.362IleGly: 4.362 ± 0.083	1.0IleHis: 1.0 ± 0.025	1.705IleIle: 1.705 ± 0.049	0.858IleLys: 0.858 ± 0.037	3.321IleLeu: 3.321 ± 0.06	0.527IleMet: 0.527 ± 0.029	1.108IleAsn: 1.108 ± 0.039	2.26IlePro: 2.26 ± 0.049	1.113IleGln: 1.113 ± 0.035	2.975IleArg: 2.975 ± 0.054	2.225IleSer: 2.225 ± 0.051	2.779IleThr: 2.779 ± 0.056	4.129IleVal: 4.129 ± 0.07	0.365IleTrp: 0.365 ± 0.021	1.041IleTyr: 1.041 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	1.853LysAla: 1.853 ± 0.058	0.155LysCys: 0.155 ± 0.013	1.033LysAsp: 1.033 ± 0.039	1.467LysGlu: 1.467 ± 0.042	0.531LysPhe: 0.531 ± 0.025	1.415LysGly: 1.415 ± 0.043	0.545LysHis: 0.545 ± 0.024	0.836LysIle: 0.836 ± 0.032	0.621LysLys: 0.621 ± 0.03	1.681LysLeu: 1.681 ± 0.053	0.346LysMet: 0.346 ± 0.02	0.645LysAsn: 0.645 ± 0.031	0.981LysPro: 0.981 ± 0.035	0.816LysGln: 0.816 ± 0.034	2.01LysArg: 2.01 ± 0.058	1.155LysSer: 1.155 ± 0.041	1.349LysThr: 1.349 ± 0.038	1.17LysVal: 1.17 ± 0.038	0.235LysTrp: 0.235 ± 0.018	0.628LysTyr: 0.628 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	10.676LeuAla: 10.676 ± 0.142	0.637LeuCys: 0.637 ± 0.025	7.15LeuAsp: 7.15 ± 0.099	6.58LeuGlu: 6.58 ± 0.104	3.049LeuPhe: 3.049 ± 0.066	7.135LeuGly: 7.135 ± 0.104	1.525LeuHis: 1.525 ± 0.039	3.936LeuIle: 3.936 ± 0.067	1.772LeuLys: 1.772 ± 0.054	8.232LeuLeu: 8.232 ± 0.119	1.296LeuMet: 1.296 ± 0.036	1.841LeuAsn: 1.841 ± 0.044	4.09LeuPro: 4.09 ± 0.068	1.997LeuGln: 1.997 ± 0.052	5.918LeuArg: 5.918 ± 0.079	5.513LeuSer: 5.513 ± 0.096	5.345LeuThr: 5.345 ± 0.092	7.618LeuVal: 7.618 ± 0.091	0.877LeuTrp: 0.877 ± 0.032	2.084LeuTyr: 2.084 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.806MetAla: 1.806 ± 0.047	0.13MetCys: 0.13 ± 0.011	1.232MetAsp: 1.232 ± 0.039	1.189MetGlu: 1.189 ± 0.039	0.496MetPhe: 0.496 ± 0.025	1.312MetGly: 1.312 ± 0.036	0.332MetHis: 0.332 ± 0.016	0.748MetIle: 0.748 ± 0.029	0.461MetLys: 0.461 ± 0.022	1.377MetLeu: 1.377 ± 0.038	0.318MetMet: 0.318 ± 0.019	0.59MetAsn: 0.59 ± 0.021	0.776MetPro: 0.776 ± 0.031	0.482MetGln: 0.482 ± 0.024	1.101MetArg: 1.101 ± 0.032	1.459MetSer: 1.459 ± 0.037	1.537MetThr: 1.537 ± 0.038	1.167MetVal: 1.167 ± 0.036	0.151MetTrp: 0.151 ± 0.014	0.439MetTyr: 0.439 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.527AsnAla: 2.527 ± 0.057	0.198AsnCys: 0.198 ± 0.015	1.784AsnAsp: 1.784 ± 0.045	1.883AsnGlu: 1.883 ± 0.049	0.582AsnPhe: 0.582 ± 0.025	1.978AsnGly: 1.978 ± 0.049	0.495AsnHis: 0.495 ± 0.027	0.927AsnIle: 0.927 ± 0.038	0.547AsnLys: 0.547 ± 0.026	1.93AsnLeu: 1.93 ± 0.053	0.365AsnMet: 0.365 ± 0.019	0.587AsnAsn: 0.587 ± 0.031	1.538AsnPro: 1.538 ± 0.037	0.636AsnGln: 0.636 ± 0.028	1.681AsnArg: 1.681 ± 0.039	0.942AsnSer: 0.942 ± 0.03	1.289AsnThr: 1.289 ± 0.045	2.214AsnVal: 2.214 ± 0.045	0.306AsnTrp: 0.306 ± 0.018	0.66AsnTyr: 0.66 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	4.868ProAla: 4.868 ± 0.089	0.263ProCys: 0.263 ± 0.018	4.755ProAsp: 4.755 ± 0.08	4.879ProGlu: 4.879 ± 0.078	1.694ProPhe: 1.694 ± 0.042	3.865ProGly: 3.865 ± 0.076	0.945ProHis: 0.945 ± 0.033	2.329ProIle: 2.329 ± 0.054	0.871ProLys: 0.871 ± 0.03	3.624ProLeu: 3.624 ± 0.064	0.862ProMet: 0.862 ± 0.028	1.12ProAsn: 1.12 ± 0.03	2.334ProPro: 2.334 ± 0.057	0.867ProGln: 0.867 ± 0.029	2.369ProArg: 2.369 ± 0.048	2.639ProSer: 2.639 ± 0.056	3.323ProThr: 3.323 ± 0.067	4.195ProVal: 4.195 ± 0.059	0.5ProTrp: 0.5 ± 0.023	1.158ProTyr: 1.158 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.156GlnAla: 2.156 ± 0.053	0.154GlnCys: 0.154 ± 0.013	1.209GlnAsp: 1.209 ± 0.036	1.595GlnGlu: 1.595 ± 0.052	0.857GlnPhe: 0.857 ± 0.03	1.495GlnGly: 1.495 ± 0.042	0.513GlnHis: 0.513 ± 0.024	1.151GlnIle: 1.151 ± 0.036	0.593GlnLys: 0.593 ± 0.028	2.177GlnLeu: 2.177 ± 0.055	0.432GlnMet: 0.432 ± 0.023	0.61GlnAsn: 0.61 ± 0.025	1.118GlnPro: 1.118 ± 0.036	0.878GlnGln: 0.878 ± 0.041	1.883GlnArg: 1.883 ± 0.051	1.272GlnSer: 1.272 ± 0.04	1.36GlnThr: 1.36 ± 0.036	1.629GlnVal: 1.629 ± 0.042	0.316GlnTrp: 0.316 ± 0.022	0.772GlnTyr: 0.772 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	6.5ArgAla: 6.5 ± 0.094	0.509ArgCys: 0.509 ± 0.024	5.086ArgAsp: 5.086 ± 0.081	6.974ArgGlu: 6.974 ± 0.109	2.635ArgPhe: 2.635 ± 0.052	4.663ArgGly: 4.663 ± 0.075	1.321ArgHis: 1.321 ± 0.041	3.62ArgIle: 3.62 ± 0.062	1.585ArgLys: 1.585 ± 0.048	6.41ArgLeu: 6.41 ± 0.097	1.478ArgMet: 1.478 ± 0.036	1.798ArgAsn: 1.798 ± 0.045	2.801ArgPro: 2.801 ± 0.055	1.668ArgGln: 1.668 ± 0.043	5.49ArgArg: 5.49 ± 0.091	3.839ArgSer: 3.839 ± 0.069	4.444ArgThr: 4.444 ± 0.069	5.445ArgVal: 5.445 ± 0.084	0.861ArgTrp: 0.861 ± 0.031	2.11ArgTyr: 2.11 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	4.939SerAla: 4.939 ± 0.071	0.354SerCys: 0.354 ± 0.021	4.288SerAsp: 4.288 ± 0.073	4.291SerGlu: 4.291 ± 0.077	1.864SerPhe: 1.864 ± 0.052	4.976SerGly: 4.976 ± 0.085	0.946SerHis: 0.946 ± 0.029	2.66SerIle: 2.66 ± 0.052	1.277SerLys: 1.277 ± 0.039	4.538SerLeu: 4.538 ± 0.071	1.083SerMet: 1.083 ± 0.031	1.342SerAsn: 1.342 ± 0.036	2.562SerPro: 2.562 ± 0.047	1.184SerGln: 1.184 ± 0.04	3.343SerArg: 3.343 ± 0.065	2.735SerSer: 2.735 ± 0.062	3.614SerThr: 3.614 ± 0.07	4.512SerVal: 4.512 ± 0.077	0.61SerTrp: 0.61 ± 0.026	1.288SerTyr: 1.288 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	6.973ThrAla: 6.973 ± 0.087	0.39ThrCys: 0.39 ± 0.02	5.853ThrAsp: 5.853 ± 0.092	5.012ThrGlu: 5.012 ± 0.084	2.325ThrPhe: 2.325 ± 0.056	5.615ThrGly: 5.615 ± 0.095	1.337ThrHis: 1.337 ± 0.036	3.132ThrIle: 3.132 ± 0.058	1.062ThrLys: 1.062 ± 0.04	6.034ThrLeu: 6.034 ± 0.099	1.02ThrMet: 1.02 ± 0.033	1.488ThrAsn: 1.488 ± 0.033	3.319ThrPro: 3.319 ± 0.063	1.337ThrGln: 1.337 ± 0.04	3.61ThrArg: 3.61 ± 0.069	2.873ThrSer: 2.873 ± 0.059	4.337ThrThr: 4.337 ± 0.084	6.846ThrVal: 6.846 ± 0.114	0.628ThrTrp: 0.628 ± 0.026	1.802ThrTyr: 1.802 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	9.925ValAla: 9.925 ± 0.12	0.684ValCys: 0.684 ± 0.029	7.599ValAsp: 7.599 ± 0.117	7.845ValGlu: 7.845 ± 0.119	2.901ValPhe: 2.901 ± 0.059	7.337ValGly: 7.337 ± 0.104	1.725ValHis: 1.725 ± 0.044	4.077ValIle: 4.077 ± 0.072	1.485ValLys: 1.485 ± 0.046	7.41ValLeu: 7.41 ± 0.089	1.317ValMet: 1.317 ± 0.04	1.924ValAsn: 1.924 ± 0.045	4.119ValPro: 4.119 ± 0.066	1.711ValGln: 1.711 ± 0.047	5.404ValArg: 5.404 ± 0.082	4.816ValSer: 4.816 ± 0.075	5.938ValThr: 5.938 ± 0.083	8.244ValVal: 8.244 ± 0.107	0.75ValTrp: 0.75 ± 0.029	2.015ValTyr: 2.015 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.874TrpAla: 0.874 ± 0.031	0.104TrpCys: 0.104 ± 0.01	0.745TrpAsp: 0.745 ± 0.028	0.892TrpGlu: 0.892 ± 0.032	0.443TrpPhe: 0.443 ± 0.024	0.791TrpGly: 0.791 ± 0.03	0.246TrpHis: 0.246 ± 0.016	0.607TrpIle: 0.607 ± 0.023	0.281TrpLys: 0.281 ± 0.017	1.177TrpLeu: 1.177 ± 0.041	0.238TrpMet: 0.238 ± 0.018	0.346TrpAsn: 0.346 ± 0.02	0.404TrpPro: 0.404 ± 0.023	0.327TrpGln: 0.327 ± 0.018	0.919TrpArg: 0.919 ± 0.035	0.596TrpSer: 0.596 ± 0.028	0.704TrpThr: 0.704 ± 0.029	0.822TrpVal: 0.822 ± 0.035	0.165TrpTrp: 0.165 ± 0.014	0.347TrpTyr: 0.347 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.71TyrAla: 2.71 ± 0.058	0.25TyrCys: 0.25 ± 0.016	2.462TyrAsp: 2.462 ± 0.046	2.322TyrGlu: 2.322 ± 0.052	0.826TyrPhe: 0.826 ± 0.029	2.292TyrGly: 2.292 ± 0.052	0.658TyrHis: 0.658 ± 0.021	0.86TyrIle: 0.86 ± 0.026	0.501TyrLys: 0.501 ± 0.024	2.498TyrLeu: 2.498 ± 0.053	0.373TyrMet: 0.373 ± 0.023	0.686TyrAsn: 0.686 ± 0.03	1.208TyrPro: 1.208 ± 0.037	0.694TyrGln: 0.694 ± 0.028	1.984TyrArg: 1.984 ± 0.045	1.153TyrSer: 1.153 ± 0.037	1.501TyrThr: 1.501 ± 0.04	2.225TyrVal: 2.225 ± 0.044	0.325TyrTrp: 0.325 ± 0.019	0.806TyrTyr: 0.806 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3371 proteins (1002170 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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