Amino acid dipepetide frequency for Enterobacterial phage mEp390

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.335AlaAla: 11.335 ± 1.598
0.724AlaCys: 0.724 ± 0.263
5.949AlaAsp: 5.949 ± 0.841
6.753AlaGlu: 6.753 ± 1.033
3.055AlaPhe: 3.055 ± 0.454
8.361AlaGly: 8.361 ± 0.835
1.769AlaHis: 1.769 ± 0.445
5.145AlaIle: 5.145 ± 0.647
4.341AlaLys: 4.341 ± 0.594
9.004AlaLeu: 9.004 ± 0.714
3.537AlaMet: 3.537 ± 0.659
4.663AlaAsn: 4.663 ± 0.651
3.135AlaPro: 3.135 ± 0.528
4.824AlaGln: 4.824 ± 0.716
5.306AlaArg: 5.306 ± 0.811
6.512AlaSer: 6.512 ± 1.311
4.904AlaThr: 4.904 ± 0.74
6.11AlaVal: 6.11 ± 0.75
1.688AlaTrp: 1.688 ± 0.366
2.814AlaTyr: 2.814 ± 0.64
0.0AlaXaa: 0.0 ± 0.0
Cys
1.045CysAla: 1.045 ± 0.302
0.241CysCys: 0.241 ± 0.134
0.884CysAsp: 0.884 ± 0.239
0.804CysGlu: 0.804 ± 0.264
0.241CysPhe: 0.241 ± 0.164
0.884CysGly: 0.884 ± 0.293
0.161CysHis: 0.161 ± 0.123
0.322CysIle: 0.322 ± 0.163
0.884CysLys: 0.884 ± 0.283
0.884CysLeu: 0.884 ± 0.386
0.161CysMet: 0.161 ± 0.116
0.965CysAsn: 0.965 ± 0.267
0.482CysPro: 0.482 ± 0.208
0.402CysGln: 0.402 ± 0.168
0.724CysArg: 0.724 ± 0.267
0.563CysSer: 0.563 ± 0.222
0.563CysThr: 0.563 ± 0.208
0.643CysVal: 0.643 ± 0.262
0.563CysTrp: 0.563 ± 0.27
0.402CysTyr: 0.402 ± 0.221
0.0CysXaa: 0.0 ± 0.0
Asp
6.351AspAla: 6.351 ± 0.693
0.804AspCys: 0.804 ± 0.296
3.618AspAsp: 3.618 ± 0.646
3.939AspGlu: 3.939 ± 0.6
1.447AspPhe: 1.447 ± 0.489
5.788AspGly: 5.788 ± 0.827
0.724AspHis: 0.724 ± 0.269
3.216AspIle: 3.216 ± 0.485
2.331AspLys: 2.331 ± 0.366
4.904AspLeu: 4.904 ± 0.568
1.608AspMet: 1.608 ± 0.489
2.251AspAsn: 2.251 ± 0.365
1.769AspPro: 1.769 ± 0.385
2.331AspGln: 2.331 ± 0.365
3.698AspArg: 3.698 ± 0.507
2.894AspSer: 2.894 ± 0.44
1.929AspThr: 1.929 ± 0.354
4.743AspVal: 4.743 ± 0.764
0.965AspTrp: 0.965 ± 0.306
2.412AspTyr: 2.412 ± 0.469
0.0AspXaa: 0.0 ± 0.0
Glu
6.19GluAla: 6.19 ± 1.035
0.482GluCys: 0.482 ± 0.21
3.216GluAsp: 3.216 ± 0.638
3.778GluGlu: 3.778 ± 0.731
2.251GluPhe: 2.251 ± 0.342
4.18GluGly: 4.18 ± 0.695
1.286GluHis: 1.286 ± 0.381
4.341GluIle: 4.341 ± 0.556
4.1GluLys: 4.1 ± 0.473
5.627GluLeu: 5.627 ± 0.655
1.447GluMet: 1.447 ± 0.346
3.216GluAsn: 3.216 ± 0.447
2.492GluPro: 2.492 ± 0.61
3.055GluGln: 3.055 ± 0.398
4.02GluArg: 4.02 ± 0.556
3.859GluSer: 3.859 ± 0.699
2.814GluThr: 2.814 ± 0.427
3.457GluVal: 3.457 ± 0.529
1.206GluTrp: 1.206 ± 0.365
1.206GluTyr: 1.206 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
2.492PheAla: 2.492 ± 0.454
0.482PheCys: 0.482 ± 0.251
2.09PheAsp: 2.09 ± 0.414
1.527PheGlu: 1.527 ± 0.436
1.125PhePhe: 1.125 ± 0.252
3.055PheGly: 3.055 ± 0.501
0.563PheHis: 0.563 ± 0.277
1.929PheIle: 1.929 ± 0.5
1.608PheLys: 1.608 ± 0.384
1.527PheLeu: 1.527 ± 0.406
0.965PheMet: 0.965 ± 0.201
2.251PheAsn: 2.251 ± 0.378
1.769PhePro: 1.769 ± 0.434
0.965PheGln: 0.965 ± 0.296
2.251PheArg: 2.251 ± 0.477
2.09PheSer: 2.09 ± 0.409
2.573PheThr: 2.573 ± 0.416
2.251PheVal: 2.251 ± 0.379
0.643PheTrp: 0.643 ± 0.231
0.884PheTyr: 0.884 ± 0.25
0.0PheXaa: 0.0 ± 0.0
Gly
6.994GlyAla: 6.994 ± 1.174
1.367GlyCys: 1.367 ± 0.378
4.984GlyAsp: 4.984 ± 0.585
4.02GlyGlu: 4.02 ± 0.588
2.975GlyPhe: 2.975 ± 0.42
6.431GlyGly: 6.431 ± 0.838
1.125GlyHis: 1.125 ± 0.308
4.904GlyIle: 4.904 ± 0.661
4.502GlyLys: 4.502 ± 0.599
6.19GlyLeu: 6.19 ± 0.831
1.929GlyMet: 1.929 ± 0.425
3.859GlyAsn: 3.859 ± 0.642
1.769GlyPro: 1.769 ± 0.458
2.653GlyGln: 2.653 ± 0.508
3.939GlyArg: 3.939 ± 0.524
5.386GlySer: 5.386 ± 0.845
4.582GlyThr: 4.582 ± 0.821
5.145GlyVal: 5.145 ± 0.64
1.447GlyTrp: 1.447 ± 0.337
1.929GlyTyr: 1.929 ± 0.388
0.0GlyXaa: 0.0 ± 0.0
His
1.688HisAla: 1.688 ± 0.385
0.0HisCys: 0.0 ± 0.0
1.206HisAsp: 1.206 ± 0.365
1.286HisGlu: 1.286 ± 0.318
0.161HisPhe: 0.161 ± 0.114
1.367HisGly: 1.367 ± 0.389
0.643HisHis: 0.643 ± 0.23
0.965HisIle: 0.965 ± 0.284
0.643HisLys: 0.643 ± 0.173
1.769HisLeu: 1.769 ± 0.517
0.322HisMet: 0.322 ± 0.165
0.884HisAsn: 0.884 ± 0.31
0.643HisPro: 0.643 ± 0.232
0.965HisGln: 0.965 ± 0.256
1.367HisArg: 1.367 ± 0.348
1.286HisSer: 1.286 ± 0.352
0.884HisThr: 0.884 ± 0.222
0.563HisVal: 0.563 ± 0.201
0.241HisTrp: 0.241 ± 0.142
0.322HisTyr: 0.322 ± 0.148
0.0HisXaa: 0.0 ± 0.0
Ile
5.547IleAla: 5.547 ± 0.696
0.482IleCys: 0.482 ± 0.255
4.1IleAsp: 4.1 ± 0.517
3.537IleGlu: 3.537 ± 0.476
2.09IlePhe: 2.09 ± 0.363
3.376IleGly: 3.376 ± 0.553
1.045IleHis: 1.045 ± 0.428
2.814IleIle: 2.814 ± 0.433
2.171IleLys: 2.171 ± 0.382
4.02IleLeu: 4.02 ± 0.816
1.527IleMet: 1.527 ± 0.418
2.171IleAsn: 2.171 ± 0.469
2.814IlePro: 2.814 ± 0.544
2.492IleGln: 2.492 ± 0.542
2.894IleArg: 2.894 ± 0.613
5.226IleSer: 5.226 ± 0.612
3.537IleThr: 3.537 ± 0.494
3.135IleVal: 3.135 ± 0.576
0.965IleTrp: 0.965 ± 0.292
1.045IleTyr: 1.045 ± 0.267
0.0IleXaa: 0.0 ± 0.0
Lys
5.226LysAla: 5.226 ± 0.708
0.563LysCys: 0.563 ± 0.202
3.457LysAsp: 3.457 ± 0.723
3.216LysGlu: 3.216 ± 0.575
1.608LysPhe: 1.608 ± 0.395
2.894LysGly: 2.894 ± 0.596
0.563LysHis: 0.563 ± 0.204
2.171LysIle: 2.171 ± 0.529
3.135LysLys: 3.135 ± 0.607
3.939LysLeu: 3.939 ± 0.445
1.286LysMet: 1.286 ± 0.35
1.849LysAsn: 1.849 ± 0.292
2.894LysPro: 2.894 ± 0.49
2.894LysGln: 2.894 ± 0.47
2.331LysArg: 2.331 ± 0.47
3.778LysSer: 3.778 ± 0.593
3.939LysThr: 3.939 ± 0.603
3.698LysVal: 3.698 ± 0.481
1.286LysTrp: 1.286 ± 0.248
1.527LysTyr: 1.527 ± 0.312
0.0LysXaa: 0.0 ± 0.0
Leu
8.843LeuAla: 8.843 ± 1.131
1.769LeuCys: 1.769 ± 0.48
4.422LeuAsp: 4.422 ± 0.393
5.226LeuGlu: 5.226 ± 0.57
1.929LeuPhe: 1.929 ± 0.472
3.457LeuGly: 3.457 ± 0.684
0.965LeuHis: 0.965 ± 0.273
4.422LeuIle: 4.422 ± 0.611
4.261LeuLys: 4.261 ± 0.695
5.949LeuLeu: 5.949 ± 0.673
1.688LeuMet: 1.688 ± 0.362
3.778LeuAsn: 3.778 ± 0.63
3.457LeuPro: 3.457 ± 0.495
3.859LeuGln: 3.859 ± 0.435
5.386LeuArg: 5.386 ± 0.668
5.467LeuSer: 5.467 ± 0.702
5.708LeuThr: 5.708 ± 0.76
5.226LeuVal: 5.226 ± 0.799
0.482LeuTrp: 0.482 ± 0.183
3.296LeuTyr: 3.296 ± 0.475
0.0LeuXaa: 0.0 ± 0.0
Met
2.975MetAla: 2.975 ± 0.433
0.402MetCys: 0.402 ± 0.163
1.125MetAsp: 1.125 ± 0.281
1.286MetGlu: 1.286 ± 0.309
0.884MetPhe: 0.884 ± 0.318
1.286MetGly: 1.286 ± 0.26
0.482MetHis: 0.482 ± 0.218
0.643MetIle: 0.643 ± 0.288
2.01MetLys: 2.01 ± 0.46
2.09MetLeu: 2.09 ± 0.424
0.563MetMet: 0.563 ± 0.237
0.965MetAsn: 0.965 ± 0.296
1.527MetPro: 1.527 ± 0.345
1.206MetGln: 1.206 ± 0.298
1.206MetArg: 1.206 ± 0.305
2.412MetSer: 2.412 ± 0.495
2.09MetThr: 2.09 ± 0.418
1.608MetVal: 1.608 ± 0.365
0.322MetTrp: 0.322 ± 0.156
0.482MetTyr: 0.482 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
4.422AsnAla: 4.422 ± 0.573
0.402AsnCys: 0.402 ± 0.183
2.412AsnAsp: 2.412 ± 0.399
3.216AsnGlu: 3.216 ± 0.453
1.206AsnPhe: 1.206 ± 0.311
4.743AsnGly: 4.743 ± 0.61
0.884AsnHis: 0.884 ± 0.275
2.331AsnIle: 2.331 ± 0.488
2.331AsnLys: 2.331 ± 0.405
2.573AsnLeu: 2.573 ± 0.544
1.045AsnMet: 1.045 ± 0.251
2.894AsnAsn: 2.894 ± 0.519
2.814AsnPro: 2.814 ± 0.657
1.929AsnGln: 1.929 ± 0.373
1.769AsnArg: 1.769 ± 0.484
2.733AsnSer: 2.733 ± 0.401
3.296AsnThr: 3.296 ± 0.623
2.492AsnVal: 2.492 ± 0.495
1.125AsnTrp: 1.125 ± 0.258
1.045AsnTyr: 1.045 ± 0.382
0.0AsnXaa: 0.0 ± 0.0
Pro
3.939ProAla: 3.939 ± 0.678
0.482ProCys: 0.482 ± 0.238
2.492ProAsp: 2.492 ± 0.606
3.618ProGlu: 3.618 ± 0.717
1.286ProPhe: 1.286 ± 0.41
3.457ProGly: 3.457 ± 0.391
0.884ProHis: 0.884 ± 0.27
1.688ProIle: 1.688 ± 0.408
2.01ProLys: 2.01 ± 0.39
3.457ProLeu: 3.457 ± 0.609
0.965ProMet: 0.965 ± 0.292
1.125ProAsn: 1.125 ± 0.347
1.929ProPro: 1.929 ± 0.402
2.492ProGln: 2.492 ± 0.38
2.251ProArg: 2.251 ± 0.408
2.894ProSer: 2.894 ± 0.6
1.769ProThr: 1.769 ± 0.339
3.698ProVal: 3.698 ± 0.562
0.563ProTrp: 0.563 ± 0.292
0.884ProTyr: 0.884 ± 0.243
0.0ProXaa: 0.0 ± 0.0
Gln
5.467GlnAla: 5.467 ± 0.807
0.322GlnCys: 0.322 ± 0.179
0.643GlnAsp: 0.643 ± 0.207
2.492GlnGlu: 2.492 ± 0.456
1.769GlnPhe: 1.769 ± 0.36
2.171GlnGly: 2.171 ± 0.385
0.804GlnHis: 0.804 ± 0.296
2.412GlnIle: 2.412 ± 0.359
2.492GlnLys: 2.492 ± 0.492
3.698GlnLeu: 3.698 ± 0.488
1.125GlnMet: 1.125 ± 0.324
2.01GlnAsn: 2.01 ± 0.42
2.331GlnPro: 2.331 ± 0.442
2.653GlnGln: 2.653 ± 0.547
3.537GlnArg: 3.537 ± 0.743
3.296GlnSer: 3.296 ± 0.593
3.135GlnThr: 3.135 ± 0.456
3.055GlnVal: 3.055 ± 0.461
0.804GlnTrp: 0.804 ± 0.273
1.206GlnTyr: 1.206 ± 0.342
0.0GlnXaa: 0.0 ± 0.0
Arg
4.904ArgAla: 4.904 ± 0.577
0.724ArgCys: 0.724 ± 0.292
3.537ArgAsp: 3.537 ± 0.583
3.618ArgGlu: 3.618 ± 0.677
2.573ArgPhe: 2.573 ± 0.415
3.618ArgGly: 3.618 ± 0.555
1.206ArgHis: 1.206 ± 0.359
4.18ArgIle: 4.18 ± 0.666
3.537ArgLys: 3.537 ± 0.593
5.065ArgLeu: 5.065 ± 0.62
1.367ArgMet: 1.367 ± 0.342
3.055ArgAsn: 3.055 ± 0.488
1.849ArgPro: 1.849 ± 0.414
2.251ArgGln: 2.251 ± 0.419
3.859ArgArg: 3.859 ± 0.719
2.09ArgSer: 2.09 ± 0.352
3.457ArgThr: 3.457 ± 0.487
4.502ArgVal: 4.502 ± 0.655
1.206ArgTrp: 1.206 ± 0.271
1.608ArgTyr: 1.608 ± 0.425
0.0ArgXaa: 0.0 ± 0.0
Ser
5.788SerAla: 5.788 ± 0.787
0.643SerCys: 0.643 ± 0.261
4.422SerAsp: 4.422 ± 0.714
3.778SerGlu: 3.778 ± 0.513
2.171SerPhe: 2.171 ± 0.321
6.512SerGly: 6.512 ± 0.898
1.286SerHis: 1.286 ± 0.309
2.975SerIle: 2.975 ± 0.462
3.216SerLys: 3.216 ± 0.573
5.869SerLeu: 5.869 ± 0.668
1.849SerMet: 1.849 ± 0.4
2.573SerAsn: 2.573 ± 0.462
2.653SerPro: 2.653 ± 0.446
4.1SerGln: 4.1 ± 0.55
3.859SerArg: 3.859 ± 0.549
4.984SerSer: 4.984 ± 0.907
3.537SerThr: 3.537 ± 0.625
5.226SerVal: 5.226 ± 0.908
1.608SerTrp: 1.608 ± 0.326
1.045SerTyr: 1.045 ± 0.279
0.0SerXaa: 0.0 ± 0.0
Thr
5.627ThrAla: 5.627 ± 0.69
0.563ThrCys: 0.563 ± 0.242
3.537ThrAsp: 3.537 ± 0.471
3.618ThrGlu: 3.618 ± 0.494
2.573ThrPhe: 2.573 ± 0.546
5.547ThrGly: 5.547 ± 0.787
0.884ThrHis: 0.884 ± 0.235
3.859ThrIle: 3.859 ± 0.55
2.975ThrLys: 2.975 ± 0.533
3.939ThrLeu: 3.939 ± 0.436
1.206ThrMet: 1.206 ± 0.317
2.171ThrAsn: 2.171 ± 0.379
2.573ThrPro: 2.573 ± 0.501
2.09ThrGln: 2.09 ± 0.647
3.135ThrArg: 3.135 ± 0.406
4.904ThrSer: 4.904 ± 0.592
3.376ThrThr: 3.376 ± 0.496
4.02ThrVal: 4.02 ± 0.469
1.125ThrTrp: 1.125 ± 0.331
1.125ThrTyr: 1.125 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
6.753ValAla: 6.753 ± 0.655
0.482ValCys: 0.482 ± 0.199
3.296ValAsp: 3.296 ± 0.517
3.939ValGlu: 3.939 ± 0.576
2.251ValPhe: 2.251 ± 0.402
4.984ValGly: 4.984 ± 0.761
0.643ValHis: 0.643 ± 0.243
4.341ValIle: 4.341 ± 0.594
3.859ValLys: 3.859 ± 0.558
5.949ValLeu: 5.949 ± 0.894
1.769ValMet: 1.769 ± 0.342
3.618ValAsn: 3.618 ± 0.66
2.894ValPro: 2.894 ± 0.464
2.412ValGln: 2.412 ± 0.399
3.135ValArg: 3.135 ± 0.463
5.145ValSer: 5.145 ± 0.87
4.02ValThr: 4.02 ± 0.533
5.306ValVal: 5.306 ± 0.794
1.125ValTrp: 1.125 ± 0.293
1.608ValTyr: 1.608 ± 0.43
0.0ValXaa: 0.0 ± 0.0
Trp
1.447TrpAla: 1.447 ± 0.382
0.563TrpCys: 0.563 ± 0.239
1.125TrpAsp: 1.125 ± 0.281
1.125TrpGlu: 1.125 ± 0.276
0.563TrpPhe: 0.563 ± 0.232
1.527TrpGly: 1.527 ± 0.398
0.563TrpHis: 0.563 ± 0.189
0.965TrpIle: 0.965 ± 0.355
0.804TrpLys: 0.804 ± 0.269
1.447TrpLeu: 1.447 ± 0.335
0.884TrpMet: 0.884 ± 0.263
0.724TrpAsn: 0.724 ± 0.233
0.804TrpPro: 0.804 ± 0.209
0.482TrpGln: 0.482 ± 0.173
1.045TrpArg: 1.045 ± 0.277
0.804TrpSer: 0.804 ± 0.268
1.286TrpThr: 1.286 ± 0.351
1.286TrpVal: 1.286 ± 0.267
0.563TrpTrp: 0.563 ± 0.189
0.322TrpTyr: 0.322 ± 0.139
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.894TyrAla: 2.894 ± 0.499
0.322TyrCys: 0.322 ± 0.157
1.206TyrAsp: 1.206 ± 0.257
1.447TyrGlu: 1.447 ± 0.354
0.884TyrPhe: 0.884 ± 0.283
2.492TyrGly: 2.492 ± 0.402
0.724TyrHis: 0.724 ± 0.261
1.367TyrIle: 1.367 ± 0.314
1.045TyrLys: 1.045 ± 0.233
1.688TyrLeu: 1.688 ± 0.41
0.241TyrMet: 0.241 ± 0.125
0.643TyrAsn: 0.643 ± 0.194
1.286TyrPro: 1.286 ± 0.385
1.367TyrGln: 1.367 ± 0.298
2.492TyrArg: 2.492 ± 0.58
1.769TyrSer: 1.769 ± 0.471
1.527TyrThr: 1.527 ± 0.395
1.367TyrVal: 1.367 ± 0.312
0.402TyrTrp: 0.402 ± 0.167
0.402TyrTyr: 0.402 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (12440 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski