Streptomyces sp. CB01883

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7450 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q5KU05|A0A1Q5KU05_9ACTN Dehydratase OS=Streptomyces sp. CB01883 OX=1703943 GN=AMK32_13810 PE=3 SV=1
MM1 pKa = 7.36IRR3 pKa = 11.84KK4 pKa = 8.74CALALGAILLASGCGGSSQHH24 pKa = 6.04EE25 pKa = 4.56SGAGRR30 pKa = 11.84SPSVDD35 pKa = 2.81ASTSSASDD43 pKa = 3.46TASSTPEE50 pKa = 3.7ATGSSPEE57 pKa = 3.91PALTTEE63 pKa = 4.55EE64 pKa = 4.19PAEE67 pKa = 4.05EE68 pKa = 4.19TSAEE72 pKa = 4.14EE73 pKa = 4.46TPASDD78 pKa = 5.23PLDD81 pKa = 4.14SDD83 pKa = 4.86DD84 pKa = 6.61GEE86 pKa = 4.68DD87 pKa = 3.55TTDD90 pKa = 5.44DD91 pKa = 3.73DD92 pKa = 4.74TEE94 pKa = 4.2PAGDD98 pKa = 4.2GSVDD102 pKa = 3.61CAGLAGSGTFTDD114 pKa = 4.14PLRR117 pKa = 11.84LGSLDD122 pKa = 3.63GPVTARR128 pKa = 11.84GCAPLTPGSPFNVRR142 pKa = 11.84YY143 pKa = 9.51FSFTLSGSPGSNAYY157 pKa = 10.12AGASFTLTQDD167 pKa = 2.98ALGPVYY173 pKa = 9.87PAIVQPNGWVLKK185 pKa = 10.15HH186 pKa = 6.17SLGSGYY192 pKa = 7.46WTGTEE197 pKa = 3.69PDD199 pKa = 3.11FTGRR203 pKa = 11.84YY204 pKa = 6.36QQIGDD209 pKa = 4.73LAPGTYY215 pKa = 9.55ILRR218 pKa = 11.84TEE220 pKa = 4.32KK221 pKa = 10.83LDD223 pKa = 3.79TALHH227 pKa = 6.01SMTTPSYY234 pKa = 11.2DD235 pKa = 3.35VVVDD239 pKa = 4.38PDD241 pKa = 3.61SSSYY245 pKa = 11.24

Molecular weight: 24.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q5KGS6|A0A1Q5KGS6_9ACTN Uncharacterized protein OS=Streptomyces sp. CB01883 OX=1703943 GN=AMK32_23810 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.1VRR4 pKa = 11.84KK5 pKa = 9.01SLRR8 pKa = 11.84SLKK11 pKa = 9.9SQPGAQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GVVFVVNKK30 pKa = 10.1KK31 pKa = 9.66NPRR34 pKa = 11.84LKK36 pKa = 10.61ARR38 pKa = 11.84QGG40 pKa = 3.23

Molecular weight: 4.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7450

0

7450

2405307

34

7422

322.9

34.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.79 ± 0.041

0.793 ± 0.009

6.0 ± 0.022

5.601 ± 0.029

2.721 ± 0.017

9.577 ± 0.029

2.38 ± 0.015

2.957 ± 0.021

2.116 ± 0.022

10.443 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.686 ± 0.012

1.682 ± 0.014

6.108 ± 0.029

2.688 ± 0.016

8.248 ± 0.03

4.867 ± 0.02

6.148 ± 0.028

8.573 ± 0.028

1.516 ± 0.01

2.106 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski