Saccharopolyspora antimicrobica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharopolyspora

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7655 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5KXV0|A0A1I5KXV0_9PSEU Nucleoside-diphosphate-sugar epimerase OS=Saccharopolyspora antimicrobica OX=455193 GN=SAMN05421805_12847 PE=4 SV=1
MM1 pKa = 6.3TTPYY5 pKa = 10.61PEE7 pKa = 5.14ALSQALDD14 pKa = 3.64HH15 pKa = 7.27PDD17 pKa = 3.19VKK19 pKa = 11.33VEE21 pKa = 4.17TTTDD25 pKa = 3.54SPDD28 pKa = 3.95GQCEE32 pKa = 3.79WCQMVGALCAVSVEE46 pKa = 4.14WLPRR50 pKa = 11.84PDD52 pKa = 3.61VLSDD56 pKa = 3.42EE57 pKa = 4.43STEE60 pKa = 4.1ACLDD64 pKa = 3.77CAASRR69 pKa = 11.84VRR71 pKa = 11.84WAVDD75 pKa = 3.26DD76 pKa = 4.83ALDD79 pKa = 4.05GSVVRR84 pKa = 11.84VEE86 pKa = 4.42VAGGAAAA93 pKa = 4.55

Molecular weight: 9.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4XAE3|A0A1I4XAE3_9PSEU Putative membrane protein insertion efficiency factor OS=Saccharopolyspora antimicrobica OX=455193 GN=SAMN05421805_103323 PE=3 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 8.65HH19 pKa = 4.47GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVASRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84QGRR42 pKa = 11.84ARR44 pKa = 11.84LTAA47 pKa = 4.17

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7655

0

7655

2397594

25

8307

313.2

33.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.199 ± 0.04

0.809 ± 0.008

5.905 ± 0.023

6.087 ± 0.026

2.93 ± 0.016

9.069 ± 0.028

2.278 ± 0.014

3.604 ± 0.018

1.908 ± 0.02

10.561 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.798 ± 0.011

1.908 ± 0.013

5.902 ± 0.028

3.118 ± 0.018

7.975 ± 0.029

5.206 ± 0.023

5.617 ± 0.022

8.721 ± 0.025

1.532 ± 0.012

1.874 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski