Rhodohalobacter barkolensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Balneolaeota; Balneolia; Balneolales; Balneolaceae; Rhodohalobacter

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3017 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N0VFE7|A0A2N0VFE7_9BACT Uncharacterized protein OS=Rhodohalobacter barkolensis OX=2053187 GN=CWD77_12750 PE=4 SV=1
MM1 pKa = 7.5NNLKK5 pKa = 10.66VNFMKK10 pKa = 10.62PIKK13 pKa = 10.1RR14 pKa = 11.84LMLVTLIGVSFMSCDD29 pKa = 4.29DD30 pKa = 4.41FGDD33 pKa = 5.06LNDD36 pKa = 4.97DD37 pKa = 4.19PNNPSQVSPDD47 pKa = 3.44QLLTDD52 pKa = 3.91SQRR55 pKa = 11.84NMSDD59 pKa = 2.97VIGAVNGTLYY69 pKa = 10.16VQYY72 pKa = 10.34IAEE75 pKa = 4.22TQYY78 pKa = 11.41TDD80 pKa = 3.06AQEE83 pKa = 4.07YY84 pKa = 7.17RR85 pKa = 11.84TTNASFYY92 pKa = 10.52FWYY95 pKa = 9.56TDD97 pKa = 4.61PIQNLQTIIDD107 pKa = 4.51LNTDD111 pKa = 3.19EE112 pKa = 4.58EE113 pKa = 4.65TRR115 pKa = 11.84DD116 pKa = 3.37QASALGSNANQIAVARR132 pKa = 11.84ILKK135 pKa = 10.16AYY137 pKa = 8.87FYY139 pKa = 11.42QMMTDD144 pKa = 2.96RR145 pKa = 11.84WGMIPYY151 pKa = 9.82SEE153 pKa = 4.37ALQGEE158 pKa = 4.84EE159 pKa = 4.69DD160 pKa = 4.37FSPAYY165 pKa = 10.12DD166 pKa = 3.71SQEE169 pKa = 4.45DD170 pKa = 3.99IYY172 pKa = 11.22EE173 pKa = 4.42AIITEE178 pKa = 4.33LKK180 pKa = 10.14EE181 pKa = 3.84AADD184 pKa = 3.78QIEE187 pKa = 4.33LSEE190 pKa = 4.8AGVSGDD196 pKa = 3.47ILFSGDD202 pKa = 3.04MEE204 pKa = 4.32QWVLFANSLRR214 pKa = 11.84ARR216 pKa = 11.84AALRR220 pKa = 11.84IADD223 pKa = 3.63VGGDD227 pKa = 3.59VSVDD231 pKa = 3.49PAAEE235 pKa = 3.85FADD238 pKa = 4.01AVDD241 pKa = 4.99DD242 pKa = 4.22GLINSDD248 pKa = 3.32VLYY251 pKa = 9.38PYY253 pKa = 10.4QSNADD258 pKa = 4.74DD259 pKa = 3.98EE260 pKa = 4.85NPWYY264 pKa = 10.91SRR266 pKa = 11.84FQTRR270 pKa = 11.84TDD272 pKa = 3.5YY273 pKa = 11.34AISEE277 pKa = 4.53TIADD281 pKa = 3.85YY282 pKa = 10.03MKK284 pKa = 10.78GLEE287 pKa = 4.2DD288 pKa = 5.45DD289 pKa = 5.07RR290 pKa = 11.84ILVYY294 pKa = 11.0ANPAPNYY301 pKa = 10.11SNDD304 pKa = 3.74DD305 pKa = 3.55GVVTFDD311 pKa = 5.03EE312 pKa = 4.25IRR314 pKa = 11.84GMPFLEE320 pKa = 5.0DD321 pKa = 3.36AGEE324 pKa = 4.17LEE326 pKa = 4.12NAEE329 pKa = 3.91ISFPGSAIGAGGPGVGDD346 pKa = 3.25QSAPLPIITVAEE358 pKa = 3.92MHH360 pKa = 6.38FAMAEE365 pKa = 3.78AVEE368 pKa = 4.56RR369 pKa = 11.84GWITGAAADD378 pKa = 4.1YY379 pKa = 10.44YY380 pKa = 11.02YY381 pKa = 11.08DD382 pKa = 4.4GIEE385 pKa = 4.51ASWEE389 pKa = 3.66QWGVYY394 pKa = 10.33DD395 pKa = 4.25DD396 pKa = 4.73TNFADD401 pKa = 5.14YY402 pKa = 10.48IAQTEE407 pKa = 4.42VAYY410 pKa = 8.71GTDD413 pKa = 3.42DD414 pKa = 3.36WDD416 pKa = 3.96VQIGTQKK423 pKa = 10.2WIALFPHH430 pKa = 7.25GYY432 pKa = 9.61EE433 pKa = 4.86GWAEE437 pKa = 3.96WRR439 pKa = 11.84RR440 pKa = 11.84LGQPEE445 pKa = 4.35LTPNQFGVGTDD456 pKa = 3.3PQIPVRR462 pKa = 11.84FTYY465 pKa = 10.07PSSEE469 pKa = 3.72NTLNQEE475 pKa = 3.95NYY477 pKa = 8.51EE478 pKa = 4.22AAVQAQGPDD487 pKa = 3.48SPNTRR492 pKa = 11.84IWWDD496 pKa = 2.84VDD498 pKa = 3.13

Molecular weight: 55.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N0VKN7|A0A2N0VKN7_9BACT Ribose 5-phosphate isomerase B OS=Rhodohalobacter barkolensis OX=2053187 GN=rpiB PE=3 SV=1
MM1 pKa = 7.57AHH3 pKa = 7.61PKK5 pKa = 10.32RR6 pKa = 11.84KK7 pKa = 7.65TSKK10 pKa = 8.0TRR12 pKa = 11.84RR13 pKa = 11.84DD14 pKa = 3.28KK15 pKa = 10.96RR16 pKa = 11.84RR17 pKa = 11.84SHH19 pKa = 6.37HH20 pKa = 6.4RR21 pKa = 11.84VAEE24 pKa = 4.22IPLSEE29 pKa = 4.28CTNCGAMHH37 pKa = 7.43RR38 pKa = 11.84YY39 pKa = 8.46HH40 pKa = 6.61HH41 pKa = 6.56VCMEE45 pKa = 4.23CGYY48 pKa = 10.15YY49 pKa = 10.05RR50 pKa = 11.84GRR52 pKa = 11.84QVLNVSKK59 pKa = 11.04

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3017

0

3017

1043729

38

3927

345.9

38.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.565 ± 0.038

0.576 ± 0.014

6.231 ± 0.044

7.725 ± 0.047

4.813 ± 0.037

6.92 ± 0.046

1.921 ± 0.025

7.205 ± 0.037

5.586 ± 0.061

9.301 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.022

4.919 ± 0.033

3.898 ± 0.024

3.651 ± 0.027

4.683 ± 0.035

6.93 ± 0.045

5.372 ± 0.037

6.452 ± 0.038

1.199 ± 0.021

3.568 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski