Rhodomicrobium vannielii (strain ATCC 17100 / ATH 3.1.1 / DSM 162 / LMG 4299)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Rhodomicrobium; Rhodomicrobium vannielii

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3513 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3I6R9|E3I6R9_RHOVT Flagellin OS=Rhodomicrobium vannielii (strain ATCC 17100 / ATH 3.1.1 / DSM 162 / LMG 4299) OX=648757 GN=Rvan_2572 PE=3 SV=1
MM1 pKa = 7.44SLYY4 pKa = 11.17GLLNTSASGMAAQSTALSTVADD26 pKa = 4.39NIANVSTTGYY36 pKa = 9.96KK37 pKa = 9.87RR38 pKa = 11.84ASTEE42 pKa = 3.46FSTLVASSGAGNYY55 pKa = 7.93QSGGVDD61 pKa = 2.66VDD63 pKa = 3.57TRR65 pKa = 11.84VAIGSQSGTTSTSSSTDD82 pKa = 3.3LAVDD86 pKa = 3.69GNGFFIVSGPNGTPALTRR104 pKa = 11.84AGSFEE109 pKa = 3.99KK110 pKa = 10.71QADD113 pKa = 3.98GTLEE117 pKa = 3.86NAAGYY122 pKa = 7.45TLLGYY127 pKa = 9.4PVGTTPSANSYY138 pKa = 11.12AGLEE142 pKa = 4.12EE143 pKa = 4.69VNISDD148 pKa = 4.64LAMKK152 pKa = 9.94AVPSTEE158 pKa = 3.79GTVTFNLPLQATAVTDD174 pKa = 3.72ATKK177 pKa = 10.79LPSANAADD185 pKa = 3.45ATYY188 pKa = 8.83TQKK191 pKa = 9.9TSFTAYY197 pKa = 10.17NNAGTAVNLDD207 pKa = 3.12AYY209 pKa = 8.04YY210 pKa = 10.73TKK212 pKa = 10.22TADD215 pKa = 3.62NTWEE219 pKa = 3.85VSVYY223 pKa = 10.43NQADD227 pKa = 3.35ATAGGFPYY235 pKa = 10.58SSGALATEE243 pKa = 4.18TLTFDD248 pKa = 3.28STTYY252 pKa = 11.53ALTSPTSLSIPVPNGSTVVLDD273 pKa = 3.66ISKK276 pKa = 8.49STQLDD281 pKa = 3.16AKK283 pKa = 9.09YY284 pKa = 9.08TFSANVNGNAASAVKK299 pKa = 10.26SVEE302 pKa = 3.71ISDD305 pKa = 5.08DD306 pKa = 3.49GTLSVVYY313 pKa = 10.22EE314 pKa = 4.7DD315 pKa = 4.41GSSVAAYY322 pKa = 7.37TIPLAMVASPDD333 pKa = 3.3NMTVISGNAYY343 pKa = 10.44LPNQDD348 pKa = 3.93SGSVQIGTAGTNGFGTIGSYY368 pKa = 7.58TLEE371 pKa = 4.27NSDD374 pKa = 3.26VDD376 pKa = 4.22LATEE380 pKa = 4.69LTRR383 pKa = 11.84MIQSQNIYY391 pKa = 10.11QSNAKK396 pKa = 9.48VFQTGSDD403 pKa = 3.65LLKK406 pKa = 11.1VLVNLNTT413 pKa = 3.59

Molecular weight: 42.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3I8I3|E3I8I3_RHOVT Death-on-curing family protein OS=Rhodomicrobium vannielii (strain ATCC 17100 / ATH 3.1.1 / DSM 162 / LMG 4299) OX=648757 GN=Rvan_1642 PE=4 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.12RR4 pKa = 11.84TYY6 pKa = 10.35QPSNLRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.25RR15 pKa = 11.84KK16 pKa = 8.64HH17 pKa = 4.95GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 9.78QGRR29 pKa = 11.84TVLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84TKK42 pKa = 10.84LSAA45 pKa = 3.69

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3513

0

3513

1117615

30

1994

318.1

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.731 ± 0.066

0.888 ± 0.015

5.536 ± 0.03

6.001 ± 0.038

3.894 ± 0.027

8.176 ± 0.044

1.993 ± 0.023

5.196 ± 0.031

3.945 ± 0.034

9.912 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.016

2.67 ± 0.027

5.212 ± 0.033

3.013 ± 0.027

7.171 ± 0.043

5.609 ± 0.032

5.162 ± 0.031

7.181 ± 0.032

1.253 ± 0.017

2.316 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski