Amino acid dipepetide frequency for Mycobacterium phage Kersh

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.024AlaAla: 14.024 ± 1.849
1.341AlaCys: 1.341 ± 0.318
7.786AlaAsp: 7.786 ± 0.512
6.754AlaGlu: 6.754 ± 0.774
2.784AlaPhe: 2.784 ± 0.342
10.312AlaGly: 10.312 ± 1.263
2.166AlaHis: 2.166 ± 0.369
4.383AlaIle: 4.383 ± 0.607
4.331AlaLys: 4.331 ± 0.445
7.889AlaLeu: 7.889 ± 0.608
2.423AlaMet: 2.423 ± 0.405
2.836AlaAsn: 2.836 ± 0.374
5.259AlaPro: 5.259 ± 0.562
3.764AlaGln: 3.764 ± 0.442
7.682AlaArg: 7.682 ± 0.814
4.898AlaSer: 4.898 ± 0.563
6.6AlaThr: 6.6 ± 0.703
7.115AlaVal: 7.115 ± 0.646
2.733AlaTrp: 2.733 ± 0.381
2.269AlaTyr: 2.269 ± 0.308
0.0AlaXaa: 0.0 ± 0.0
Cys
1.083CysAla: 1.083 ± 0.249
0.0CysCys: 0.0 ± 0.0
1.134CysAsp: 1.134 ± 0.278
0.98CysGlu: 0.98 ± 0.267
0.155CysPhe: 0.155 ± 0.094
1.547CysGly: 1.547 ± 0.359
0.309CysHis: 0.309 ± 0.11
0.258CysIle: 0.258 ± 0.14
0.619CysLys: 0.619 ± 0.194
0.773CysLeu: 0.773 ± 0.264
0.103CysMet: 0.103 ± 0.074
0.412CysAsn: 0.412 ± 0.149
1.341CysPro: 1.341 ± 0.268
0.309CysGln: 0.309 ± 0.124
0.98CysArg: 0.98 ± 0.319
0.722CysSer: 0.722 ± 0.238
0.619CysThr: 0.619 ± 0.196
0.619CysVal: 0.619 ± 0.136
0.206CysTrp: 0.206 ± 0.095
0.206CysTyr: 0.206 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
7.476AspAla: 7.476 ± 0.486
0.722AspCys: 0.722 ± 0.194
4.434AspAsp: 4.434 ± 0.496
3.558AspGlu: 3.558 ± 0.488
1.444AspPhe: 1.444 ± 0.237
6.239AspGly: 6.239 ± 0.506
1.289AspHis: 1.289 ± 0.236
2.32AspIle: 2.32 ± 0.308
1.959AspLys: 1.959 ± 0.293
5.156AspLeu: 5.156 ± 0.545
1.341AspMet: 1.341 ± 0.269
1.65AspAsn: 1.65 ± 0.363
4.176AspPro: 4.176 ± 0.565
2.217AspGln: 2.217 ± 0.321
5.517AspArg: 5.517 ± 0.542
3.661AspSer: 3.661 ± 0.477
4.743AspThr: 4.743 ± 0.42
4.486AspVal: 4.486 ± 0.448
1.341AspTrp: 1.341 ± 0.239
1.908AspTyr: 1.908 ± 0.339
0.0AspXaa: 0.0 ± 0.0
Glu
5.775GluAla: 5.775 ± 0.592
0.928GluCys: 0.928 ± 0.241
3.506GluAsp: 3.506 ± 0.394
2.423GluGlu: 2.423 ± 0.442
1.908GluPhe: 1.908 ± 0.365
3.506GluGly: 3.506 ± 0.467
1.444GluHis: 1.444 ± 0.303
2.217GluIle: 2.217 ± 0.319
1.908GluLys: 1.908 ± 0.354
5.362GluLeu: 5.362 ± 0.677
1.65GluMet: 1.65 ± 0.29
1.856GluAsn: 1.856 ± 0.292
3.3GluPro: 3.3 ± 0.412
2.733GluGln: 2.733 ± 0.405
4.795GluArg: 4.795 ± 0.591
2.784GluSer: 2.784 ± 0.47
4.383GluThr: 4.383 ± 0.548
3.867GluVal: 3.867 ± 0.425
1.495GluTrp: 1.495 ± 0.24
1.908GluTyr: 1.908 ± 0.347
0.0GluXaa: 0.0 ± 0.0
Phe
2.63PheAla: 2.63 ± 0.428
0.206PheCys: 0.206 ± 0.097
2.372PheAsp: 2.372 ± 0.368
1.753PheGlu: 1.753 ± 0.278
0.567PhePhe: 0.567 ± 0.171
2.217PheGly: 2.217 ± 0.54
0.464PheHis: 0.464 ± 0.142
1.341PheIle: 1.341 ± 0.308
1.031PheLys: 1.031 ± 0.234
1.908PheLeu: 1.908 ± 0.275
0.619PheMet: 0.619 ± 0.156
1.031PheAsn: 1.031 ± 0.214
1.495PhePro: 1.495 ± 0.247
1.134PheGln: 1.134 ± 0.335
1.289PheArg: 1.289 ± 0.239
1.083PheSer: 1.083 ± 0.265
2.63PheThr: 2.63 ± 0.348
1.908PheVal: 1.908 ± 0.277
0.567PheTrp: 0.567 ± 0.136
0.67PheTyr: 0.67 ± 0.19
0.0PheXaa: 0.0 ± 0.0
Gly
9.075GlyAla: 9.075 ± 1.121
1.289GlyCys: 1.289 ± 0.298
5.104GlyAsp: 5.104 ± 0.443
5.053GlyGlu: 5.053 ± 0.593
2.269GlyPhe: 2.269 ± 0.411
10.157GlyGly: 10.157 ± 1.865
1.65GlyHis: 1.65 ± 0.286
3.042GlyIle: 3.042 ± 0.459
2.475GlyLys: 2.475 ± 0.372
5.414GlyLeu: 5.414 ± 0.479
1.547GlyMet: 1.547 ± 0.363
3.197GlyAsn: 3.197 ± 0.453
4.537GlyPro: 4.537 ± 0.701
2.526GlyGln: 2.526 ± 0.563
5.672GlyArg: 5.672 ± 0.568
6.703GlySer: 6.703 ± 1.055
6.548GlyThr: 6.548 ± 0.738
6.342GlyVal: 6.342 ± 0.654
2.836GlyTrp: 2.836 ± 0.404
1.959GlyTyr: 1.959 ± 0.303
0.0GlyXaa: 0.0 ± 0.0
His
2.062HisAla: 2.062 ± 0.308
0.309HisCys: 0.309 ± 0.123
0.773HisAsp: 0.773 ± 0.213
1.186HisGlu: 1.186 ± 0.227
0.516HisPhe: 0.516 ± 0.159
1.856HisGly: 1.856 ± 0.286
0.825HisHis: 0.825 ± 0.286
1.341HisIle: 1.341 ± 0.297
0.98HisLys: 0.98 ± 0.183
1.495HisLeu: 1.495 ± 0.265
0.516HisMet: 0.516 ± 0.163
1.083HisAsn: 1.083 ± 0.236
1.547HisPro: 1.547 ± 0.267
0.825HisGln: 0.825 ± 0.212
2.062HisArg: 2.062 ± 0.377
0.877HisSer: 0.877 ± 0.202
1.392HisThr: 1.392 ± 0.313
1.341HisVal: 1.341 ± 0.304
0.567HisTrp: 0.567 ± 0.166
0.773HisTyr: 0.773 ± 0.199
0.0HisXaa: 0.0 ± 0.0
Ile
4.898IleAla: 4.898 ± 0.454
0.412IleCys: 0.412 ± 0.164
3.97IleAsp: 3.97 ± 0.498
2.99IleGlu: 2.99 ± 0.317
0.619IlePhe: 0.619 ± 0.156
3.815IleGly: 3.815 ± 0.374
1.547IleHis: 1.547 ± 0.321
1.547IleIle: 1.547 ± 0.257
1.134IleLys: 1.134 ± 0.24
2.269IleLeu: 2.269 ± 0.439
0.412IleMet: 0.412 ± 0.161
1.908IleAsn: 1.908 ± 0.325
2.681IlePro: 2.681 ± 0.319
1.392IleGln: 1.392 ± 0.25
2.217IleArg: 2.217 ± 0.319
1.805IleSer: 1.805 ± 0.383
4.176IleThr: 4.176 ± 0.489
2.99IleVal: 2.99 ± 0.338
0.722IleTrp: 0.722 ± 0.221
0.928IleTyr: 0.928 ± 0.193
0.0IleXaa: 0.0 ± 0.0
Lys
4.022LysAla: 4.022 ± 0.506
0.516LysCys: 0.516 ± 0.157
1.341LysAsp: 1.341 ± 0.262
1.186LysGlu: 1.186 ± 0.251
1.134LysPhe: 1.134 ± 0.213
2.526LysGly: 2.526 ± 0.314
1.134LysHis: 1.134 ± 0.316
0.98LysIle: 0.98 ± 0.241
1.598LysLys: 1.598 ± 0.428
2.99LysLeu: 2.99 ± 0.421
0.67LysMet: 0.67 ± 0.202
1.186LysAsn: 1.186 ± 0.227
3.094LysPro: 3.094 ± 0.469
1.495LysGln: 1.495 ± 0.293
2.32LysArg: 2.32 ± 0.37
2.423LysSer: 2.423 ± 0.284
1.805LysThr: 1.805 ± 0.298
2.63LysVal: 2.63 ± 0.328
0.877LysTrp: 0.877 ± 0.244
0.928LysTyr: 0.928 ± 0.239
0.0LysXaa: 0.0 ± 0.0
Leu
8.404LeuAla: 8.404 ± 0.685
0.67LeuCys: 0.67 ± 0.211
4.537LeuAsp: 4.537 ± 0.549
4.125LeuGlu: 4.125 ± 0.532
2.062LeuPhe: 2.062 ± 0.3
4.847LeuGly: 4.847 ± 0.528
1.134LeuHis: 1.134 ± 0.244
3.145LeuIle: 3.145 ± 0.368
2.578LeuLys: 2.578 ± 0.428
5.208LeuLeu: 5.208 ± 0.664
1.908LeuMet: 1.908 ± 0.298
2.887LeuAsn: 2.887 ± 0.362
5.62LeuPro: 5.62 ± 0.675
2.733LeuGln: 2.733 ± 0.404
4.898LeuArg: 4.898 ± 0.616
4.898LeuSer: 4.898 ± 0.474
5.208LeuThr: 5.208 ± 0.552
5.001LeuVal: 5.001 ± 0.509
1.237LeuTrp: 1.237 ± 0.262
2.114LeuTyr: 2.114 ± 0.343
0.0LeuXaa: 0.0 ± 0.0
Met
2.269MetAla: 2.269 ± 0.259
0.258MetCys: 0.258 ± 0.183
1.134MetAsp: 1.134 ± 0.273
1.134MetGlu: 1.134 ± 0.236
0.516MetPhe: 0.516 ± 0.203
1.341MetGly: 1.341 ± 0.3
0.103MetHis: 0.103 ± 0.07
0.67MetIle: 0.67 ± 0.178
0.567MetLys: 0.567 ± 0.16
1.908MetLeu: 1.908 ± 0.269
0.464MetMet: 0.464 ± 0.235
0.928MetAsn: 0.928 ± 0.201
1.392MetPro: 1.392 ± 0.223
0.67MetGln: 0.67 ± 0.237
1.65MetArg: 1.65 ± 0.26
2.526MetSer: 2.526 ± 0.318
2.011MetThr: 2.011 ± 0.326
1.341MetVal: 1.341 ± 0.282
0.258MetTrp: 0.258 ± 0.116
0.309MetTyr: 0.309 ± 0.116
0.0MetXaa: 0.0 ± 0.0
Asn
3.403AsnAla: 3.403 ± 0.395
0.309AsnCys: 0.309 ± 0.112
1.444AsnAsp: 1.444 ± 0.241
1.547AsnGlu: 1.547 ± 0.299
0.722AsnPhe: 0.722 ± 0.219
4.743AsnGly: 4.743 ± 0.542
0.928AsnHis: 0.928 ± 0.224
1.701AsnIle: 1.701 ± 0.342
1.237AsnLys: 1.237 ± 0.255
2.939AsnLeu: 2.939 ± 0.383
0.619AsnMet: 0.619 ± 0.178
1.547AsnAsn: 1.547 ± 0.394
2.578AsnPro: 2.578 ± 0.405
1.031AsnGln: 1.031 ± 0.265
2.114AsnArg: 2.114 ± 0.334
1.444AsnSer: 1.444 ± 0.281
2.32AsnThr: 2.32 ± 0.328
2.166AsnVal: 2.166 ± 0.298
0.877AsnTrp: 0.877 ± 0.189
0.773AsnTyr: 0.773 ± 0.22
0.0AsnXaa: 0.0 ± 0.0
Pro
5.62ProAla: 5.62 ± 0.606
0.67ProCys: 0.67 ± 0.19
4.176ProAsp: 4.176 ± 0.498
4.64ProGlu: 4.64 ± 0.432
1.701ProPhe: 1.701 ± 0.301
6.29ProGly: 6.29 ± 0.666
1.547ProHis: 1.547 ± 0.282
2.114ProIle: 2.114 ± 0.267
2.423ProLys: 2.423 ± 0.406
4.847ProLeu: 4.847 ± 0.581
1.392ProMet: 1.392 ± 0.334
2.269ProAsn: 2.269 ± 0.287
3.815ProPro: 3.815 ± 0.577
2.217ProGln: 2.217 ± 0.442
3.454ProArg: 3.454 ± 0.408
3.764ProSer: 3.764 ± 0.398
4.125ProThr: 4.125 ± 0.575
4.434ProVal: 4.434 ± 0.421
1.289ProTrp: 1.289 ± 0.24
1.753ProTyr: 1.753 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
3.919GlnAla: 3.919 ± 0.488
0.412GlnCys: 0.412 ± 0.21
1.701GlnAsp: 1.701 ± 0.273
1.856GlnGlu: 1.856 ± 0.333
1.237GlnPhe: 1.237 ± 0.234
2.681GlnGly: 2.681 ± 0.564
1.031GlnHis: 1.031 ± 0.229
1.753GlnIle: 1.753 ± 0.331
1.134GlnLys: 1.134 ± 0.195
2.784GlnLeu: 2.784 ± 0.514
0.773GlnMet: 0.773 ± 0.203
1.083GlnAsn: 1.083 ± 0.297
2.578GlnPro: 2.578 ± 0.383
1.031GlnGln: 1.031 ± 0.232
2.217GlnArg: 2.217 ± 0.357
2.166GlnSer: 2.166 ± 0.33
1.547GlnThr: 1.547 ± 0.276
2.578GlnVal: 2.578 ± 0.378
0.877GlnTrp: 0.877 ± 0.215
0.877GlnTyr: 0.877 ± 0.262
0.0GlnXaa: 0.0 ± 0.0
Arg
6.961ArgAla: 6.961 ± 0.715
1.65ArgCys: 1.65 ± 0.376
4.795ArgAsp: 4.795 ± 0.535
5.053ArgGlu: 5.053 ± 0.599
1.908ArgPhe: 1.908 ± 0.342
3.919ArgGly: 3.919 ± 0.465
1.289ArgHis: 1.289 ± 0.272
3.919ArgIle: 3.919 ± 0.481
2.526ArgLys: 2.526 ± 0.382
5.208ArgLeu: 5.208 ± 0.597
2.32ArgMet: 2.32 ± 0.386
2.166ArgAsn: 2.166 ± 0.393
4.022ArgPro: 4.022 ± 0.449
1.753ArgGln: 1.753 ± 0.293
5.259ArgArg: 5.259 ± 0.663
3.815ArgSer: 3.815 ± 0.539
3.351ArgThr: 3.351 ± 0.485
5.208ArgVal: 5.208 ± 0.639
2.217ArgTrp: 2.217 ± 0.365
1.959ArgTyr: 1.959 ± 0.28
0.0ArgXaa: 0.0 ± 0.0
Ser
6.445SerAla: 6.445 ± 1.252
0.412SerCys: 0.412 ± 0.156
4.64SerAsp: 4.64 ± 0.5
2.733SerGlu: 2.733 ± 0.464
1.701SerPhe: 1.701 ± 0.258
6.342SerGly: 6.342 ± 0.937
0.928SerHis: 0.928 ± 0.221
2.836SerIle: 2.836 ± 0.381
2.011SerLys: 2.011 ± 0.344
3.609SerLeu: 3.609 ± 0.424
1.341SerMet: 1.341 ± 0.283
2.269SerAsn: 2.269 ± 0.304
3.506SerPro: 3.506 ± 0.394
1.908SerGln: 1.908 ± 0.278
3.712SerArg: 3.712 ± 0.451
3.867SerSer: 3.867 ± 0.575
3.094SerThr: 3.094 ± 0.421
3.815SerVal: 3.815 ± 0.463
0.98SerTrp: 0.98 ± 0.218
1.805SerTyr: 1.805 ± 0.287
0.0SerXaa: 0.0 ± 0.0
Thr
6.806ThrAla: 6.806 ± 0.554
0.722ThrCys: 0.722 ± 0.247
4.331ThrAsp: 4.331 ± 0.596
4.022ThrGlu: 4.022 ± 0.423
1.959ThrPhe: 1.959 ± 0.377
6.6ThrGly: 6.6 ± 0.726
1.805ThrHis: 1.805 ± 0.272
3.558ThrIle: 3.558 ± 0.462
1.959ThrLys: 1.959 ± 0.294
3.712ThrLeu: 3.712 ± 0.485
1.392ThrMet: 1.392 ± 0.274
2.423ThrAsn: 2.423 ± 0.448
4.692ThrPro: 4.692 ± 0.544
2.011ThrGln: 2.011 ± 0.231
4.434ThrArg: 4.434 ± 0.565
3.558ThrSer: 3.558 ± 0.462
5.053ThrThr: 5.053 ± 0.806
6.342ThrVal: 6.342 ± 0.558
1.392ThrTrp: 1.392 ± 0.349
1.753ThrTyr: 1.753 ± 0.293
0.0ThrXaa: 0.0 ± 0.0
Val
7.734ValAla: 7.734 ± 0.553
1.134ValCys: 1.134 ± 0.217
5.053ValAsp: 5.053 ± 0.561
4.073ValGlu: 4.073 ± 0.456
2.32ValPhe: 2.32 ± 0.302
4.95ValGly: 4.95 ± 0.559
1.701ValHis: 1.701 ± 0.311
3.145ValIle: 3.145 ± 0.412
2.887ValLys: 2.887 ± 0.422
5.672ValLeu: 5.672 ± 0.612
0.98ValMet: 0.98 ± 0.199
1.959ValAsn: 1.959 ± 0.34
4.022ValPro: 4.022 ± 0.356
2.475ValGln: 2.475 ± 0.297
4.589ValArg: 4.589 ± 0.55
4.847ValSer: 4.847 ± 0.647
5.362ValThr: 5.362 ± 0.643
6.6ValVal: 6.6 ± 0.656
1.495ValTrp: 1.495 ± 0.252
1.392ValTyr: 1.392 ± 0.223
0.0ValXaa: 0.0 ± 0.0
Trp
2.423TrpAla: 2.423 ± 0.315
0.258TrpCys: 0.258 ± 0.107
1.598TrpAsp: 1.598 ± 0.299
1.083TrpGlu: 1.083 ± 0.252
0.67TrpPhe: 0.67 ± 0.182
1.237TrpGly: 1.237 ± 0.21
0.567TrpHis: 0.567 ± 0.164
1.134TrpIle: 1.134 ± 0.239
0.722TrpLys: 0.722 ± 0.207
1.908TrpLeu: 1.908 ± 0.361
0.773TrpMet: 0.773 ± 0.251
0.567TrpAsn: 0.567 ± 0.171
1.444TrpPro: 1.444 ± 0.273
1.134TrpGln: 1.134 ± 0.277
2.526TrpArg: 2.526 ± 0.392
1.237TrpSer: 1.237 ± 0.238
1.753TrpThr: 1.753 ± 0.287
1.341TrpVal: 1.341 ± 0.3
0.877TrpTrp: 0.877 ± 0.188
0.412TrpTyr: 0.412 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.372TyrAla: 2.372 ± 0.367
0.155TyrCys: 0.155 ± 0.095
1.805TyrAsp: 1.805 ± 0.346
1.598TyrGlu: 1.598 ± 0.286
0.722TyrPhe: 0.722 ± 0.21
2.269TyrGly: 2.269 ± 0.383
0.464TyrHis: 0.464 ± 0.144
1.031TyrIle: 1.031 ± 0.196
0.773TyrLys: 0.773 ± 0.214
2.166TyrLeu: 2.166 ± 0.311
0.155TyrMet: 0.155 ± 0.087
1.186TyrAsn: 1.186 ± 0.271
1.444TyrPro: 1.444 ± 0.253
0.877TyrGln: 0.877 ± 0.188
1.908TyrArg: 1.908 ± 0.336
0.877TyrSer: 0.877 ± 0.212
1.805TyrThr: 1.805 ± 0.316
2.269TyrVal: 2.269 ± 0.34
0.825TyrTrp: 0.825 ± 0.197
0.567TyrTyr: 0.567 ± 0.155
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 107 proteins (19396 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski