Streptococcus phage Javan112

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ANY8|A0A4D6ANY8_9CAUD VRR-NUC domain-containing protein OS=Streptococcus phage Javan112 OX=2547982 GN=Javan112_0039 PE=4 SV=1
MM1 pKa = 7.48EE2 pKa = 5.54LVLMADD8 pKa = 3.51IQAPPVGTKK17 pKa = 9.05GTVRR21 pKa = 11.84GVDD24 pKa = 3.87DD25 pKa = 4.31IGSIMVSWDD34 pKa = 3.29NGSSLSVAYY43 pKa = 10.36GEE45 pKa = 4.3DD46 pKa = 3.23FCRR49 pKa = 11.84RR50 pKa = 11.84ISDD53 pKa = 3.44EE54 pKa = 3.96RR55 pKa = 4.42

Molecular weight: 5.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6APU3|A0A4D6APU3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan112 OX=2547982 GN=Javan112_0015 PE=4 SV=1
MM1 pKa = 7.64VSLKK5 pKa = 9.96QKK7 pKa = 10.35KK8 pKa = 9.18IRR10 pKa = 11.84EE11 pKa = 3.38IWAFVNFAGSQFLSLLEE28 pKa = 3.95EE29 pKa = 4.15RR30 pKa = 11.84KK31 pKa = 9.84KK32 pKa = 10.99DD33 pKa = 3.42SAQIAVEE40 pKa = 4.3TNGGIAIWMRR50 pKa = 3.49

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

16620

38

941

255.7

29.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.197 ± 0.305

1.161 ± 0.112

6.173 ± 0.218

8.171 ± 0.319

3.989 ± 0.192

6.252 ± 0.282

1.582 ± 0.099

8.026 ± 0.254

8.941 ± 0.299

8.345 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.128

5.523 ± 0.222

2.972 ± 0.188

2.948 ± 0.159

4.236 ± 0.254

6.528 ± 0.313

5.168 ± 0.204

5.824 ± 0.182

1.167 ± 0.076

4.152 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski