Enterobacteria phage mEp460

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7PJP7|K7PJP7_9CAUD Minor tail protein OS=Enterobacteria phage mEp460 OX=1147152 GN=mEp460_014 PE=4 SV=1
MM1 pKa = 8.0ADD3 pKa = 3.13PMNRR7 pKa = 11.84HH8 pKa = 4.06TQIRR12 pKa = 11.84QAVLARR18 pKa = 11.84LRR20 pKa = 11.84EE21 pKa = 4.01QCGDD25 pKa = 3.08SATFFDD31 pKa = 4.77GLPAFVDD38 pKa = 3.93AQEE41 pKa = 4.48LPAVAVWLSDD51 pKa = 3.19AQYY54 pKa = 8.79TGKK57 pKa = 7.91MTDD60 pKa = 3.19EE61 pKa = 5.38DD62 pKa = 3.58DD63 pKa = 3.67WQAVLHH69 pKa = 5.99IAVFIRR75 pKa = 11.84AQAPDD80 pKa = 3.93SEE82 pKa = 5.25LDD84 pKa = 2.98MWMEE88 pKa = 4.06STIFPALNDD97 pKa = 3.5VPALSGLIDD106 pKa = 3.52TLIPLGFNYY115 pKa = 9.81QRR117 pKa = 11.84DD118 pKa = 3.81NEE120 pKa = 4.26MATWAMAEE128 pKa = 3.65ITYY131 pKa = 10.47QITYY135 pKa = 9.38TNN137 pKa = 3.43

Molecular weight: 15.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7PH72|K7PH72_9CAUD Crossover junction endodeoxyribonuclease RusA OS=Enterobacteria phage mEp460 OX=1147152 GN=mEp460_047 PE=3 SV=1
MM1 pKa = 7.28IRR3 pKa = 11.84NIFKK7 pKa = 10.82RR8 pKa = 11.84FTNQTFRR15 pKa = 11.84CPRR18 pKa = 11.84PGQWYY23 pKa = 5.47TTPAGHH29 pKa = 6.23VLRR32 pKa = 11.84VSLVDD37 pKa = 3.87RR38 pKa = 11.84EE39 pKa = 4.3CQKK42 pKa = 10.88VICEE46 pKa = 4.01PLGRR50 pKa = 11.84NYY52 pKa = 9.75RR53 pKa = 11.84VSMPLIAFRR62 pKa = 11.84SGKK65 pKa = 8.95NMKK68 pKa = 9.95HH69 pKa = 6.22LGGAAA74 pKa = 3.13

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13806

47

1202

234.0

25.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.097 ± 0.517

1.354 ± 0.185

5.664 ± 0.242

6.034 ± 0.25

3.462 ± 0.168

7.511 ± 0.254

1.796 ± 0.19

5.02 ± 0.295

5.288 ± 0.198

7.968 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.182

4.368 ± 0.226

3.998 ± 0.304

4.397 ± 0.504

6.396 ± 0.345

6.903 ± 0.445

5.795 ± 0.434

6.859 ± 0.298

1.717 ± 0.151

2.796 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski