Flavihumibacter sp. ZG627

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Flavihumibacter; unclassified Flavihumibacter

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3302 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C1INA1|A0A0C1INA1_9BACT 2-oxoglutarate reductase OS=Flavihumibacter sp. ZG627 OX=1463156 GN=HY58_06615 PE=3 SV=1
MM1 pKa = 7.6LWTACQKK8 pKa = 10.17EE9 pKa = 4.35VSPEE13 pKa = 3.45ASIQNEE19 pKa = 4.05IEE21 pKa = 4.27SADD24 pKa = 3.66LRR26 pKa = 11.84LSGVLEE32 pKa = 4.19EE33 pKa = 6.15DD34 pKa = 3.3EE35 pKa = 4.61SHH37 pKa = 6.29WNIIPVISSQNTMLKK52 pKa = 10.09PATTGKK58 pKa = 9.43PGAGGKK64 pKa = 9.94VKK66 pKa = 10.79DD67 pKa = 4.27SDD69 pKa = 3.9GDD71 pKa = 4.33GIPDD75 pKa = 3.77TSDD78 pKa = 2.96ACPTQMEE85 pKa = 4.89TFNGYY90 pKa = 9.66QDD92 pKa = 3.74TDD94 pKa = 3.85GCPDD98 pKa = 3.77TAPVTTSPDD107 pKa = 3.01TDD109 pKa = 3.7ADD111 pKa = 4.63GIPDD115 pKa = 5.08DD116 pKa = 5.68KK117 pKa = 11.23DD118 pKa = 3.1ACPTQKK124 pKa = 9.23EE125 pKa = 4.57TVNGYY130 pKa = 10.29EE131 pKa = 5.19DD132 pKa = 5.55GDD134 pKa = 4.14GCPDD138 pKa = 3.8TVPPPTATDD147 pKa = 3.46TDD149 pKa = 4.06GDD151 pKa = 4.93GITDD155 pKa = 4.15DD156 pKa = 3.86QDD158 pKa = 3.6GCITQKK164 pKa = 9.41EE165 pKa = 4.59TVNGYY170 pKa = 10.2LDD172 pKa = 4.62NDD174 pKa = 4.29GCPDD178 pKa = 3.75TVPVVTEE185 pKa = 4.01PTTLPSSYY193 pKa = 10.37SLVMPPVSNQGGEE206 pKa = 4.41GSCVAFSVTYY216 pKa = 10.24ARR218 pKa = 11.84SYY220 pKa = 10.29EE221 pKa = 3.88QFKK224 pKa = 10.94RR225 pKa = 11.84SGATSYY231 pKa = 10.85SQSTNIMSPEE241 pKa = 3.85FVFNQIKK248 pKa = 10.59VDD250 pKa = 4.04VNCSASSLLSAYY262 pKa = 10.43DD263 pKa = 3.31FLVYY267 pKa = 10.72NGVCTWQSMPYY278 pKa = 8.89TSSNGCSLMPTTEE291 pKa = 4.07QLSEE295 pKa = 3.85ALNYY299 pKa = 9.61KK300 pKa = 9.73IKK302 pKa = 10.54AYY304 pKa = 10.6SQVLDD309 pKa = 3.77TDD311 pKa = 3.6ITAIKK316 pKa = 9.84QMIYY320 pKa = 10.81AKK322 pKa = 10.17RR323 pKa = 11.84PLVGQFSIDD332 pKa = 3.41DD333 pKa = 4.08GFRR336 pKa = 11.84TAGPGYY342 pKa = 9.19IWKK345 pKa = 8.72TLGSNTGLHH354 pKa = 5.69TLVICGYY361 pKa = 10.77DD362 pKa = 3.45DD363 pKa = 3.24TKK365 pKa = 11.34KK366 pKa = 10.73AFKK369 pKa = 10.54VLNSWGTGWGDD380 pKa = 3.53SGYY383 pKa = 11.25GWVDD387 pKa = 3.17YY388 pKa = 10.32TFLASVSSKK397 pKa = 10.66LLVMEE402 pKa = 4.96FF403 pKa = 3.59

Molecular weight: 43.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C1L2I2|A0A0C1L2I2_9BACT Uncharacterized protein OS=Flavihumibacter sp. ZG627 OX=1463156 GN=HY58_14830 PE=4 SV=1
MM1 pKa = 7.23MMTMVINSRR10 pKa = 11.84IIRR13 pKa = 11.84YY14 pKa = 8.73PLMAMVIMSVCAMMIDD30 pKa = 3.4IVVMPEE36 pKa = 3.58QGVMMMIDD44 pKa = 3.91AVPIKK49 pKa = 7.75TLRR52 pKa = 11.84MIRR55 pKa = 11.84RR56 pKa = 11.84GHH58 pKa = 5.89KK59 pKa = 10.15AIHH62 pKa = 6.03

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3302

0

3302

1139701

35

2898

345.2

38.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.627 ± 0.052

0.82 ± 0.018

5.263 ± 0.027

5.978 ± 0.044

4.791 ± 0.032

7.172 ± 0.043

1.959 ± 0.025

7.251 ± 0.033

6.343 ± 0.039

9.555 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.02

5.183 ± 0.04

4.018 ± 0.023

3.668 ± 0.027

4.559 ± 0.029

6.414 ± 0.034

5.412 ± 0.036

6.271 ± 0.029

1.296 ± 0.019

3.792 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski