Turnip yellow mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Tymoviridae; Tymovirus

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P10357|P69_TYMV 69 kDa protein OS=Turnip yellow mosaic virus OX=12154 PE=3 SV=2
MM1 pKa = 7.68EE2 pKa = 5.82IDD4 pKa = 5.4KK5 pKa = 10.81EE6 pKa = 4.04LAPQDD11 pKa = 3.45RR12 pKa = 11.84TVTVATVLPAVPGPSPLTIKK32 pKa = 10.6QPFQSEE38 pKa = 4.48VLFAGTKK45 pKa = 9.9DD46 pKa = 3.53AEE48 pKa = 4.39ASLTIANIDD57 pKa = 3.76SVSTLTTFYY66 pKa = 10.64RR67 pKa = 11.84HH68 pKa = 6.58ASLEE72 pKa = 4.36SLWVTIHH79 pKa = 6.63PTLQAPTFPTTVGVCWVPANSPVTPAQITKK109 pKa = 9.53TYY111 pKa = 9.75GGQIFCIGGAINTLSPLIVKK131 pKa = 10.21CPLEE135 pKa = 4.08MMNPRR140 pKa = 11.84VKK142 pKa = 10.86DD143 pKa = 3.51SIQYY147 pKa = 10.45LDD149 pKa = 3.8SPKK152 pKa = 10.8LLISITAQPTAPPASTCIITVSGTLSMHH180 pKa = 6.42SPLITDD186 pKa = 4.11TSTT189 pKa = 2.91

Molecular weight: 20.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P10358|POLR_TYMV RNA replicase polyprotein OS=Turnip yellow mosaic virus OX=12154 PE=1 SV=2
MM1 pKa = 7.67SNGLPISIGRR11 pKa = 11.84PCTHH15 pKa = 7.56DD16 pKa = 3.44SQRR19 pKa = 11.84SLSAPDD25 pKa = 3.61SRR27 pKa = 11.84IHH29 pKa = 7.34SGFNSLLDD37 pKa = 3.41TDD39 pKa = 4.66LPMVHH44 pKa = 6.66SEE46 pKa = 4.23GTSTPTQLLRR56 pKa = 11.84HH57 pKa = 5.45PNIWFGNLPPPPRR70 pKa = 11.84RR71 pKa = 11.84PQDD74 pKa = 3.28NRR76 pKa = 11.84DD77 pKa = 3.55FSPLHH82 pKa = 5.93PLVFPGHH89 pKa = 6.53HH90 pKa = 5.29SQLRR94 pKa = 11.84HH95 pKa = 4.0VHH97 pKa = 5.28EE98 pKa = 4.55TQQVQQTCPGKK109 pKa = 10.63LKK111 pKa = 10.96LSGAEE116 pKa = 4.1EE117 pKa = 4.7LPPAPQRR124 pKa = 11.84QHH126 pKa = 6.46SLPLHH131 pKa = 5.27ITRR134 pKa = 11.84PSRR137 pKa = 11.84FPHH140 pKa = 6.41HH141 pKa = 5.71FHH143 pKa = 7.14ARR145 pKa = 11.84RR146 pKa = 11.84PDD148 pKa = 3.56VLPSVPDD155 pKa = 3.51HH156 pKa = 6.61GPVLTEE162 pKa = 3.93TKK164 pKa = 9.78PRR166 pKa = 11.84TSVRR170 pKa = 11.84QPRR173 pKa = 11.84SATRR177 pKa = 11.84GPSFRR182 pKa = 11.84PILLPKK188 pKa = 9.61VVHH191 pKa = 5.68VHH193 pKa = 7.4DD194 pKa = 5.53DD195 pKa = 3.82PPHH198 pKa = 6.27SSLRR202 pKa = 11.84PRR204 pKa = 11.84GSRR207 pKa = 11.84SRR209 pKa = 11.84QLQPTVRR216 pKa = 11.84RR217 pKa = 11.84PLLAPNQFHH226 pKa = 7.17SPRR229 pKa = 11.84QPPPLSDD236 pKa = 3.92DD237 pKa = 3.9PGILGPRR244 pKa = 11.84PLAPHH249 pKa = 5.92STRR252 pKa = 11.84DD253 pKa = 3.59PPPRR257 pKa = 11.84PITPGPSNTHH267 pKa = 6.23DD268 pKa = 3.9LRR270 pKa = 11.84PLSVLPRR277 pKa = 11.84TSPRR281 pKa = 11.84RR282 pKa = 11.84GLLPNPRR289 pKa = 11.84RR290 pKa = 11.84HH291 pKa = 5.34RR292 pKa = 11.84TSTGHH297 pKa = 6.63IPPTTTSRR305 pKa = 11.84PTGPPSRR312 pKa = 11.84LQRR315 pKa = 11.84PVHH318 pKa = 6.67LYY320 pKa = 9.82QSSPHH325 pKa = 5.95TPNFRR330 pKa = 11.84PSSIRR335 pKa = 11.84KK336 pKa = 8.93DD337 pKa = 3.31ALLQTGPRR345 pKa = 11.84LGHH348 pKa = 6.28LEE350 pKa = 4.36RR351 pKa = 11.84LGQPANLRR359 pKa = 11.84TSEE362 pKa = 4.21RR363 pKa = 11.84SPPTKK368 pKa = 9.85RR369 pKa = 11.84RR370 pKa = 11.84LPRR373 pKa = 11.84SSEE376 pKa = 3.89PNRR379 pKa = 11.84LPKK382 pKa = 9.87PLPEE386 pKa = 4.17ATLAPSYY393 pKa = 9.89RR394 pKa = 11.84HH395 pKa = 5.78RR396 pKa = 11.84RR397 pKa = 11.84PYY399 pKa = 10.22PLLPNPPAALPSIAYY414 pKa = 6.94TSSRR418 pKa = 11.84GKK420 pKa = 9.52IHH422 pKa = 7.03HH423 pKa = 6.52SLPKK427 pKa = 10.26GALPKK432 pKa = 10.4EE433 pKa = 4.59GAPPPPRR440 pKa = 11.84RR441 pKa = 11.84LPSPAPRR448 pKa = 11.84PQLPLRR454 pKa = 11.84DD455 pKa = 3.83LGRR458 pKa = 11.84TPGFPTPPKK467 pKa = 9.58TPTRR471 pKa = 11.84TPEE474 pKa = 3.76SRR476 pKa = 11.84ITASPTDD483 pKa = 3.88IAPLDD488 pKa = 4.03SDD490 pKa = 3.83PVLSVRR496 pKa = 11.84TEE498 pKa = 3.44VHH500 pKa = 4.98APEE503 pKa = 5.1RR504 pKa = 11.84RR505 pKa = 11.84TFMDD509 pKa = 4.06PEE511 pKa = 4.22ALRR514 pKa = 11.84SALASLPSPPRR525 pKa = 11.84SVGIIHH531 pKa = 6.71TAPQTVLPANPPSPTRR547 pKa = 11.84HH548 pKa = 6.24LPPTSPPWILQSPVGEE564 pKa = 4.12DD565 pKa = 3.79AIVDD569 pKa = 4.1SEE571 pKa = 4.73DD572 pKa = 4.07DD573 pKa = 4.22SISSFHH579 pKa = 6.75SHH581 pKa = 7.42DD582 pKa = 3.78FDD584 pKa = 4.65SPSGPLRR591 pKa = 11.84SQSPSRR597 pKa = 11.84FRR599 pKa = 11.84LHH601 pKa = 6.4LRR603 pKa = 11.84SPSTSSGIEE612 pKa = 3.64PWSPASYY619 pKa = 10.53DD620 pKa = 3.57YY621 pKa = 11.63GSAPDD626 pKa = 3.51TDD628 pKa = 3.6

Molecular weight: 69.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2661

189

1844

887.0

98.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.975 ± 0.744

1.052 ± 0.375

4.697 ± 0.256

3.232 ± 0.233

3.758 ± 0.773

3.758 ± 0.483

4.848 ± 0.812

4.397 ± 1.048

2.931 ± 0.515

11.687 ± 1.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.24 ± 0.362

3.006 ± 0.502

12.815 ± 2.841

4.021 ± 0.156

6.389 ± 2.133

10.635 ± 0.583

7.929 ± 1.428

4.096 ± 0.826

1.127 ± 0.288

2.405 ± 0.703
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski