Rat stool-associated circular ssDNA virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; Porprismacovirus ratas1

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2LV72|A0A0S2LV72_9VIRU Viral_Rep domain-containing protein OS=Rat stool-associated circular ssDNA virus OX=1699316 PE=4 SV=1
MM1 pKa = 7.37SAADD5 pKa = 3.58MTTAFWGMVINNYY18 pKa = 10.65DD19 pKa = 3.74EE20 pKa = 4.46TDD22 pKa = 3.46LALVQQGYY30 pKa = 8.97PDD32 pKa = 5.33HH33 pKa = 6.71IRR35 pKa = 11.84QMVYY39 pKa = 9.14TLEE42 pKa = 4.8KK43 pKa = 10.5GEE45 pKa = 4.65SGTPHH50 pKa = 5.22IQAYY54 pKa = 8.53VRR56 pKa = 11.84LYY58 pKa = 8.93RR59 pKa = 11.84QQRR62 pKa = 11.84LSYY65 pKa = 10.03VKK67 pKa = 10.43KK68 pKa = 10.22LFPRR72 pKa = 11.84GSFKK76 pKa = 11.11ALLAEE81 pKa = 4.42EE82 pKa = 4.6YY83 pKa = 10.51KK84 pKa = 11.22LNAQRR89 pKa = 11.84YY90 pKa = 6.01AQKK93 pKa = 10.59LDD95 pKa = 3.27KK96 pKa = 9.89TAEE99 pKa = 3.98SSAVITNNPFPDD111 pKa = 4.02PVQEE115 pKa = 3.94LLAVIRR121 pKa = 11.84DD122 pKa = 3.84GFEE125 pKa = 4.1TFHH128 pKa = 6.85EE129 pKa = 4.64SSDD132 pKa = 3.48PAKK135 pKa = 9.55WSKK138 pKa = 10.32YY139 pKa = 8.93DD140 pKa = 3.59KK141 pKa = 10.69RR142 pKa = 11.84DD143 pKa = 3.11VAAWKK148 pKa = 10.53DD149 pKa = 3.45SIEE152 pKa = 4.03RR153 pKa = 11.84VRR155 pKa = 11.84VEE157 pKa = 4.58EE158 pKa = 4.35KK159 pKa = 10.36PNLAKK164 pKa = 10.43FYY166 pKa = 10.92VSPTYY171 pKa = 9.99TRR173 pKa = 11.84VWAKK177 pKa = 10.14FGPSLIRR184 pKa = 11.84HH185 pKa = 5.42VVAQVQSEE193 pKa = 4.3RR194 pKa = 11.84GNSGHH199 pKa = 5.74THH201 pKa = 5.14THH203 pKa = 3.78THH205 pKa = 5.25TDD207 pKa = 3.23EE208 pKa = 4.39KK209 pKa = 10.64FSRR212 pKa = 11.84PGCITINAPSSEE224 pKa = 3.78GSEE227 pKa = 4.32EE228 pKa = 4.08GSEE231 pKa = 4.02ACSPGRR237 pKa = 11.84EE238 pKa = 4.06EE239 pKa = 6.0EE240 pKa = 4.39DD241 pKa = 4.88DD242 pKa = 3.74EE243 pKa = 7.36DD244 pKa = 5.56YY245 pKa = 11.67DD246 pKa = 4.91DD247 pKa = 5.36GSGSEE252 pKa = 4.52TEE254 pKa = 4.67ASTEE258 pKa = 4.25GDD260 pKa = 3.27DD261 pKa = 4.46SGCSEE266 pKa = 4.67SDD268 pKa = 3.9AEE270 pKa = 5.22SEE272 pKa = 4.55DD273 pKa = 3.62

Molecular weight: 30.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2LV72|A0A0S2LV72_9VIRU Viral_Rep domain-containing protein OS=Rat stool-associated circular ssDNA virus OX=1699316 PE=4 SV=1
MM1 pKa = 7.95LIMVEE6 pKa = 4.41LRR8 pKa = 11.84PTGIRR13 pKa = 11.84RR14 pKa = 11.84TPCLRR19 pKa = 11.84RR20 pKa = 11.84LFLSLSRR27 pKa = 11.84RR28 pKa = 11.84PSSFTRR34 pKa = 11.84RR35 pKa = 11.84LRR37 pKa = 11.84TFTPRR42 pKa = 11.84ITLRR46 pKa = 11.84CMLSDD51 pKa = 3.77MCLMTRR57 pKa = 11.84PRR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84TTSPLTICLVRR72 pKa = 11.84WSMLLRR78 pKa = 11.84ISCGSRR84 pKa = 11.84TISEE88 pKa = 4.74KK89 pKa = 10.45IFSPFIGACFGSHH102 pKa = 6.17QEE104 pKa = 4.0RR105 pKa = 11.84CPVAGNRR112 pKa = 11.84PGGSGSSRR120 pKa = 11.84PSACRR125 pKa = 11.84TQQ127 pKa = 3.1

Molecular weight: 14.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

400

127

273

200.0

22.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.25 ± 1.92

2.75 ± 1.752

5.5 ± 2.327

7.75 ± 2.66

3.75 ± 0.481

6.25 ± 0.024

2.25 ± 0.722

4.25 ± 1.012

4.0 ± 1.586

7.0 ± 1.987

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 1.098

2.25 ± 0.722

5.75 ± 1.049

3.25 ± 0.827

9.5 ± 4.251

10.75 ± 0.913

7.25 ± 1.086

4.75 ± 1.179

1.25 ± 0.228

3.0 ± 1.481
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski