Nonlabens sp. MB-3u-79

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Nonlabens; unclassified Nonlabens

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2710 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8X8N8|A0A2K8X8N8_9FLAO DUF2179 domain-containing protein OS=Nonlabens sp. MB-3u-79 OX=2058134 GN=CW736_07850 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.41KK3 pKa = 10.47LYY5 pKa = 9.71TILAALFLTASAFAQAPEE23 pKa = 4.04KK24 pKa = 10.07MSYY27 pKa = 9.19QAVVRR32 pKa = 11.84DD33 pKa = 3.69SGDD36 pKa = 3.42ALVTSQPVGMQISILQTTATGTAVYY61 pKa = 10.71VEE63 pKa = 4.59TQTPTTNVNGLVSLEE78 pKa = 4.09IGTGSVVSGDD88 pKa = 3.49FTSIDD93 pKa = 3.55WSTDD97 pKa = 2.5SYY99 pKa = 11.09FIKK102 pKa = 10.12TEE104 pKa = 3.84TDD106 pKa = 3.15PAGGTTYY113 pKa = 10.35TITGTSQLMSVPFALYY129 pKa = 10.65AKK131 pKa = 9.6TSGNGAGPIGPQGPAGNDD149 pKa = 3.51GATGPIGADD158 pKa = 3.26GAAGTNGTDD167 pKa = 3.46GAQGIQGAQGDD178 pKa = 4.03IGAAGADD185 pKa = 3.91GAQGIQGADD194 pKa = 3.25GATGTTGQQGPAGNDD209 pKa = 3.37GATGQQGLAGNDD221 pKa = 3.5GATGLTGPVGPTGPAGSLGPQGIQGAAGINGTPGIQGAPGTAGAPGVDD269 pKa = 3.8GAAGTNGTDD278 pKa = 3.46GAQGIQGADD287 pKa = 3.25GATGTTGQQGPAGNDD302 pKa = 3.21GTTGQQGLAGNDD314 pKa = 3.5GATGLTGLVGPAGSLGPQGIQGAAGINGTPGIQGATGASGADD356 pKa = 3.7GAAGAPGTAGAPGVDD371 pKa = 3.8GAAGTNGIDD380 pKa = 3.54GTQGIQGATGADD392 pKa = 4.05GATGPSGTNGTQGIQGATGADD413 pKa = 3.8GAQGIQGATGADD425 pKa = 3.8GAQGIQGEE433 pKa = 4.46TGAAGTNATQPTYY446 pKa = 10.18TVNTLYY452 pKa = 10.26PEE454 pKa = 4.29LGGYY458 pKa = 9.32VIEE461 pKa = 4.66INADD465 pKa = 3.44GTHH468 pKa = 6.22GLVAAMQDD476 pKa = 3.13QGTSNWYY483 pKa = 8.69QANDD487 pKa = 3.93LLSNAANHH495 pKa = 6.57DD496 pKa = 3.81VNGAKK501 pKa = 10.36FKK503 pKa = 10.62DD504 pKa = 3.15WRR506 pKa = 11.84IPTKK510 pKa = 10.63RR511 pKa = 11.84EE512 pKa = 3.36LNLIYY517 pKa = 9.88FQRR520 pKa = 11.84ASIGAASYY528 pKa = 9.25YY529 pKa = 9.58WSSTEE534 pKa = 3.56IDD536 pKa = 4.89NYY538 pKa = 9.06SAWYY542 pKa = 10.23QFFPNGNQGYY552 pKa = 7.77SFKK555 pKa = 11.14SSTEE559 pKa = 3.5YY560 pKa = 10.6VRR562 pKa = 11.84AVRR565 pKa = 11.84AFF567 pKa = 3.27

Molecular weight: 54.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8X8G8|A0A2K8X8G8_9FLAO Por_Secre_tail domain-containing protein OS=Nonlabens sp. MB-3u-79 OX=2058134 GN=CW736_07495 PE=4 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.63LKK7 pKa = 10.48PVTPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGYY21 pKa = 9.53DD22 pKa = 4.32AITTDD27 pKa = 3.72KK28 pKa = 11.01PEE30 pKa = 4.36KK31 pKa = 10.55SLLAPIKK38 pKa = 10.49RR39 pKa = 11.84SGGRR43 pKa = 11.84NDD45 pKa = 3.41SGRR48 pKa = 11.84MTMRR52 pKa = 11.84YY53 pKa = 9.61KK54 pKa = 10.94GGGHH58 pKa = 5.71KK59 pKa = 9.74RR60 pKa = 11.84RR61 pKa = 11.84YY62 pKa = 9.75RR63 pKa = 11.84IIDD66 pKa = 3.8FKK68 pKa = 10.97RR69 pKa = 11.84NHH71 pKa = 6.8HH72 pKa = 5.87GVPATVASIEE82 pKa = 4.02YY83 pKa = 10.1DD84 pKa = 3.45PNRR87 pKa = 11.84TAFIALVNYY96 pKa = 9.88QDD98 pKa = 3.41GEE100 pKa = 3.91KK101 pKa = 10.21RR102 pKa = 11.84YY103 pKa = 9.73IVAPNGLKK111 pKa = 10.19VGQNIVSGEE120 pKa = 4.1TATPEE125 pKa = 3.8VGNAMKK131 pKa = 10.7LSQIPLGSIVSCIEE145 pKa = 3.64LRR147 pKa = 11.84PGQGATIARR156 pKa = 11.84SAGSFAQLMARR167 pKa = 11.84DD168 pKa = 3.96GKK170 pKa = 10.33YY171 pKa = 9.08ATVKK175 pKa = 9.73MPSGEE180 pKa = 4.05TRR182 pKa = 11.84LILQEE187 pKa = 4.06CLATIGAVSNSDD199 pKa = 3.15HH200 pKa = 6.2QLVVSGKK207 pKa = 9.63AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVVMNPVDD229 pKa = 3.47HH230 pKa = 7.05PMGGGEE236 pKa = 4.28GKK238 pKa = 10.51SSGGHH243 pKa = 4.59PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.3GYY255 pKa = 7.46RR256 pKa = 11.84TRR258 pKa = 11.84DD259 pKa = 3.23KK260 pKa = 11.38NKK262 pKa = 10.31ASTQYY267 pKa = 10.45IIEE270 pKa = 4.13RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.07KK274 pKa = 9.82

Molecular weight: 29.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2710

0

2710

909226

38

4979

335.5

37.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.916 ± 0.049

0.771 ± 0.026

5.925 ± 0.044

6.43 ± 0.055

4.992 ± 0.043

6.525 ± 0.05

1.861 ± 0.024

7.602 ± 0.045

6.924 ± 0.072

9.324 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.377 ± 0.026

5.617 ± 0.05

3.424 ± 0.028

3.709 ± 0.028

3.698 ± 0.034

6.578 ± 0.037

5.94 ± 0.058

6.325 ± 0.037

1.07 ± 0.016

3.991 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski