Streptococcus phage IPP23

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1S5S9Z1|A0A1S5S9Z1_9CAUD Replication protein OS=Streptococcus phage IPP23 OX=1916163 GN=IPP23_00012 PE=4 SV=1
MM1 pKa = 7.73LAFNLLKK8 pKa = 9.5PTSNGEE14 pKa = 3.97NEE16 pKa = 3.98LGQEE20 pKa = 3.55IFEE23 pKa = 4.38YY24 pKa = 10.45KK25 pKa = 9.76QVAVFEE31 pKa = 5.43GYY33 pKa = 9.64MDD35 pKa = 3.76MLDD38 pKa = 3.78GSEE41 pKa = 4.3STDD44 pKa = 3.05KK45 pKa = 10.9LAYY48 pKa = 9.56IADD51 pKa = 3.94STHH54 pKa = 7.01VILTKK59 pKa = 10.75DD60 pKa = 2.97RR61 pKa = 11.84TVNAEE66 pKa = 3.83IEE68 pKa = 4.37DD69 pKa = 4.2KK70 pKa = 11.24IEE72 pKa = 4.24VNGKK76 pKa = 6.8TYY78 pKa = 10.62EE79 pKa = 3.96VTYY82 pKa = 11.13VDD84 pKa = 3.99DD85 pKa = 4.17PVNIGHH91 pKa = 6.9HH92 pKa = 5.69LEE94 pKa = 4.26IYY96 pKa = 10.61VKK98 pKa = 10.44GAHH101 pKa = 6.02

Molecular weight:
11.38 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1S5S9Z2|A0A1S5S9Z2_9CAUD Uncharacterized protein OS=Streptococcus phage IPP23 OX=1916163 GN=IPP23_00027 PE=4 SV=1
MM1 pKa = 7.1VVLANHH7 pKa = 6.84PNWQVYY13 pKa = 9.28PEE15 pKa = 4.76EE16 pKa = 3.7IAKK19 pKa = 10.29RR20 pKa = 11.84KK21 pKa = 9.12GVSRR25 pKa = 11.84PTVSKK30 pKa = 10.09YY31 pKa = 10.65FKK33 pKa = 9.88ILEE36 pKa = 3.99EE37 pKa = 4.49AGYY40 pKa = 10.18LRR42 pKa = 11.84QVRR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 8.3PPGRR51 pKa = 11.84GGSHH55 pKa = 5.6VFRR58 pKa = 11.84FFSDD62 pKa = 3.14RR63 pKa = 11.84KK64 pKa = 10.1ISDD67 pKa = 3.5FQFDD71 pKa = 3.15IMLQRR76 pKa = 11.84LDD78 pKa = 3.83KK79 pKa = 10.89AISDD83 pKa = 3.93STFEE87 pKa = 3.85II88 pKa = 4.66

Molecular weight:
10.33 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

10310

44

664

190.9

21.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.411 ± 0.333

0.601 ± 0.085

6.266 ± 0.259

8.264 ± 0.367

4.287 ± 0.25

5.829 ± 0.374

1.474 ± 0.127

7.546 ± 0.25

8.739 ± 0.456

8.555 ± 0.393

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.195

5.761 ± 0.246

2.473 ± 0.14

3.686 ± 0.198

4.268 ± 0.33

6.217 ± 0.285

6.198 ± 0.416

5.82 ± 0.268

1.28 ± 0.173

3.725 ± 0.285

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski