Paenibacillus sp. N3.4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6173 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C8LFE3|A0A5C8LFE3_9BACL MATE family efflux transporter OS=Paenibacillus sp. N3.4 OX=2603222 GN=FU659_29655 PE=4 SV=1
MM1 pKa = 7.27YY2 pKa = 10.81NLVNDD7 pKa = 4.18KK8 pKa = 11.2SGDD11 pKa = 3.9GPIQPYY17 pKa = 10.39DD18 pKa = 3.56SSDD21 pKa = 3.6SVDD24 pKa = 3.95LYY26 pKa = 11.35VISTRR31 pKa = 11.84EE32 pKa = 3.78DD33 pKa = 3.6SNFGQFIFTNAVLCAQDD50 pKa = 3.64IVSKK54 pKa = 10.94SS55 pKa = 3.15

Molecular weight: 6.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8LNE9|A0A5C8LNE9_9BACL Elongation factor G OS=Paenibacillus sp. N3.4 OX=2603222 GN=fusA PE=3 SV=1
MM1 pKa = 7.61GPTFNPNTRR10 pKa = 11.84KK11 pKa = 9.86RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 9.13NHH16 pKa = 5.06GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.0NGRR28 pKa = 11.84KK29 pKa = 8.8VLQARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.45GRR39 pKa = 11.84KK40 pKa = 8.72ILSAA44 pKa = 4.02

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6173

0

6173

1941827

21

3240

314.6

35.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.748 ± 0.042

0.746 ± 0.01

5.053 ± 0.022

6.207 ± 0.042

4.261 ± 0.026

7.027 ± 0.035

2.139 ± 0.018

7.203 ± 0.038

5.822 ± 0.031

10.006 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.813 ± 0.017

4.245 ± 0.03

3.832 ± 0.02

4.076 ± 0.023

4.403 ± 0.03

6.705 ± 0.029

5.822 ± 0.036

7.065 ± 0.026

1.28 ± 0.013

3.55 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski