Tomato leaf curl New Delhi virus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5N3Q4|V5N3Q4_9GEMI AC4 OS=Tomato leaf curl New Delhi virus 4 OX=2049944 PE=3 SV=1
MM1 pKa = 8.2RR2 pKa = 11.84DD3 pKa = 3.54PLLLAFPEE11 pKa = 4.34RR12 pKa = 11.84VHH14 pKa = 6.3GVRR17 pKa = 11.84SMLVEE22 pKa = 4.35KK23 pKa = 9.98PLQEE27 pKa = 4.35MEE29 pKa = 4.26DD30 pKa = 3.92HH31 pKa = 6.59YY32 pKa = 12.02SPDD35 pKa = 3.17TVGYY39 pKa = 10.65DD40 pKa = 4.08FIRR43 pKa = 11.84DD44 pKa = 4.27LILVLRR50 pKa = 11.84AKK52 pKa = 10.45NYY54 pKa = 8.94EE55 pKa = 4.16EE56 pKa = 3.92PTSRR60 pKa = 11.84YY61 pKa = 8.77HH62 pKa = 7.13HH63 pKa = 6.66IKK65 pKa = 10.77APIEE69 pKa = 4.56GIQICQVRR77 pKa = 11.84QPQRR81 pKa = 11.84SEE83 pKa = 3.93CSGPHH88 pKa = 6.27CPRR91 pKa = 11.84HH92 pKa = 5.71RR93 pKa = 11.84SKK95 pKa = 11.33GLDD98 pKa = 3.18QQAYY102 pKa = 6.3EE103 pKa = 4.3QKK105 pKa = 8.76TQNIQNAA112 pKa = 3.61

Molecular weight: 13.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5N3C4|V5N3C4_9GEMI AC5 OS=Tomato leaf curl New Delhi virus 4 OX=2049944 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 10.29NRR4 pKa = 11.84PADD7 pKa = 3.76IIISKK12 pKa = 9.72PPSKK16 pKa = 10.37VYY18 pKa = 10.28RR19 pKa = 11.84YY20 pKa = 10.71VKK22 pKa = 10.37FDD24 pKa = 3.6SPKK27 pKa = 9.0GASAVVPIARR37 pKa = 11.84VTEE40 pKa = 4.28AKK42 pKa = 10.31AWTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.62PRR54 pKa = 11.84IYY56 pKa = 9.1RR57 pKa = 11.84THH59 pKa = 7.45RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GRR68 pKa = 11.84EE69 pKa = 4.15GQRR72 pKa = 11.84KK73 pKa = 7.78LHH75 pKa = 6.29SIEE78 pKa = 4.2SRR80 pKa = 11.84LDD82 pKa = 3.16VYY84 pKa = 10.33RR85 pKa = 11.84TGKK88 pKa = 9.49IKK90 pKa = 10.7GVIHH94 pKa = 5.01VTRR97 pKa = 11.84EE98 pKa = 3.34IGLTHH103 pKa = 6.55SVGKK107 pKa = 10.38RR108 pKa = 11.84FGVNSVYY115 pKa = 10.77VLEE118 pKa = 4.48KK119 pKa = 10.63RR120 pKa = 11.84GIDD123 pKa = 3.34EE124 pKa = 4.49TIKK127 pKa = 10.65PKK129 pKa = 9.98SHH131 pKa = 6.92TYY133 pKa = 9.86RR134 pKa = 11.84VMFFLVRR141 pKa = 11.84DD142 pKa = 3.88RR143 pKa = 11.84RR144 pKa = 11.84PTGTPQDD151 pKa = 3.8FGDD154 pKa = 3.96VFNMFDD160 pKa = 4.34NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.6ATVTGGTYY191 pKa = 10.41ASKK194 pKa = 10.48EE195 pKa = 3.69QALVSKK201 pKa = 8.89FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1223

58

361

174.7

20.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.07 ± 0.478

2.126 ± 0.575

4.906 ± 0.362

4.252 ± 0.616

4.742 ± 0.548

6.051 ± 0.324

4.007 ± 0.587

5.478 ± 0.982

5.969 ± 0.6

6.541 ± 0.744

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.044 ± 0.388

5.07 ± 0.361

5.805 ± 0.614

4.742 ± 0.882

7.032 ± 0.879

7.522 ± 1.053

6.214 ± 0.717

6.868 ± 0.766

1.635 ± 0.309

3.925 ± 0.526
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski