Streptococcus phage Javan374

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B0S7|A0A4D6B0S7_9CAUD Endopeptidase OS=Streptococcus phage Javan374 OX=2548134 GN=Javan374_0010 PE=4 SV=1
MM1 pKa = 7.28TVQEE5 pKa = 4.28LCAKK9 pKa = 10.25EE10 pKa = 4.33GVNLCYY16 pKa = 10.1FDD18 pKa = 3.52GSNWHH23 pKa = 6.54SPGFFNPALNVLALDD38 pKa = 4.42FNLSVEE44 pKa = 4.27DD45 pKa = 3.67QKK47 pKa = 11.59QVALHH52 pKa = 5.66EE53 pKa = 4.36

Molecular weight: 5.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B234|A0A4D6B234_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage Javan374 OX=2548134 GN=Javan374_0043 PE=4 SV=1
MM1 pKa = 7.37NEE3 pKa = 3.45EE4 pKa = 4.12WKK6 pKa = 9.59TILDD10 pKa = 3.84ALDD13 pKa = 3.82YY14 pKa = 11.18DD15 pKa = 4.37VSNKK19 pKa = 10.15GNIRR23 pKa = 11.84NKK25 pKa = 8.9KK26 pKa = 5.77TKK28 pKa = 10.44KK29 pKa = 9.54NIKK32 pKa = 7.76TRR34 pKa = 11.84VVKK37 pKa = 10.6RR38 pKa = 11.84FGYY41 pKa = 10.64VLVNLQIGAKK51 pKa = 8.81GEE53 pKa = 3.81RR54 pKa = 11.84RR55 pKa = 11.84QRR57 pKa = 11.84TFRR60 pKa = 11.84VHH62 pKa = 6.81RR63 pKa = 11.84LVAKK67 pKa = 10.57AFIPNPDD74 pKa = 3.67NLPEE78 pKa = 4.0VDD80 pKa = 3.95HH81 pKa = 6.78VNGIKK86 pKa = 9.61TDD88 pKa = 3.42NRR90 pKa = 11.84AEE92 pKa = 3.96NLEE95 pKa = 4.15WVTGKK100 pKa = 10.68EE101 pKa = 4.03NTVRR105 pKa = 11.84AYY107 pKa = 9.87KK108 pKa = 10.38KK109 pKa = 9.86GLAKK113 pKa = 10.32ISSDD117 pKa = 2.98EE118 pKa = 4.08HH119 pKa = 5.52MKK121 pKa = 11.36AMTDD125 pKa = 3.34KK126 pKa = 10.4TKK128 pKa = 10.16KK129 pKa = 9.79ACVIVDD135 pKa = 3.2ILKK138 pKa = 10.07DD139 pKa = 3.22KK140 pKa = 11.02KK141 pKa = 11.08YY142 pKa = 10.6FFNTRR147 pKa = 11.84RR148 pKa = 11.84EE149 pKa = 3.67ASIFFGKK156 pKa = 9.94SYY158 pKa = 10.77SWATTLIKK166 pKa = 9.96TGIGSKK172 pKa = 10.16GRR174 pKa = 11.84YY175 pKa = 8.09YY176 pKa = 10.82GYY178 pKa = 10.59DD179 pKa = 2.75II180 pKa = 5.44

Molecular weight: 20.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12035

41

1442

207.5

23.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.78 ± 0.627

0.573 ± 0.109

6.224 ± 0.245

7.902 ± 0.382

4.645 ± 0.291

6.415 ± 0.42

1.388 ± 0.114

6.888 ± 0.327

8.55 ± 0.298

8.4 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.137

6.107 ± 0.207

2.775 ± 0.136

4.329 ± 0.222

4.146 ± 0.237

6.157 ± 0.312

5.949 ± 0.274

5.875 ± 0.212

1.072 ± 0.085

3.274 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski