Microbacterium phage Sucha

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Goodmanvirus; unclassified Goodmanvirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8MWW4|A0A4P8MWW4_9CAUD Uncharacterized protein OS=Microbacterium phage Sucha OX=2565527 GN=54 PE=4 SV=1
MM1 pKa = 7.69AEE3 pKa = 4.14CKK5 pKa = 7.82EE6 pKa = 4.63TYY8 pKa = 8.96VTTCNPAPPVFLPNCEE24 pKa = 4.53DD25 pKa = 2.91GWEE28 pKa = 4.23IGNGTAVCVTPEE40 pKa = 3.99PMADD44 pKa = 3.48LPATGGEE51 pKa = 4.21LPFVGLTVALALIAAGVAATVRR73 pKa = 11.84GPRR76 pKa = 11.84RR77 pKa = 3.29

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8MUT6|A0A4P8MUT6_9CAUD Holliday junction resolvase OS=Microbacterium phage Sucha OX=2565527 GN=38 PE=4 SV=1
MM1 pKa = 7.46NLTASQRR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 4.42VGKK13 pKa = 9.57WIYY16 pKa = 8.87RR17 pKa = 11.84ARR19 pKa = 11.84WILIAKK25 pKa = 8.57VVTEE29 pKa = 4.04ARR31 pKa = 11.84MNDD34 pKa = 3.13TTEE37 pKa = 3.85LEE39 pKa = 4.19RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84KK43 pKa = 8.35DD44 pKa = 3.29QEE46 pKa = 3.76MEE48 pKa = 3.78RR49 pKa = 11.84LQEE52 pKa = 4.07RR53 pKa = 11.84FEE55 pKa = 4.38GAVKK59 pKa = 10.47SLPIWLRR66 pKa = 11.84SEE68 pKa = 4.37RR69 pKa = 11.84FLNKK73 pKa = 9.74HH74 pKa = 4.59VLEE77 pKa = 4.25PAEE80 pKa = 4.0RR81 pKa = 11.84HH82 pKa = 5.07FKK84 pKa = 10.58RR85 pKa = 11.84VGILQCARR93 pKa = 11.84EE94 pKa = 4.28LRR96 pKa = 11.84NLADD100 pKa = 3.12TWRR103 pKa = 11.84PP104 pKa = 3.3

Molecular weight: 12.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13235

47

902

213.5

23.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.427 ± 0.441

0.635 ± 0.135

6.271 ± 0.199

6.604 ± 0.325

2.879 ± 0.241

8.961 ± 0.284

1.798 ± 0.172

4.05 ± 0.369

4.186 ± 0.246

8.243 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.896 ± 0.092

3.045 ± 0.179

5.606 ± 0.295

3.755 ± 0.208

6.604 ± 0.416

5.357 ± 0.217

6.528 ± 0.324

7.782 ± 0.321

2.607 ± 0.272

2.765 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski