Amino acid dipepetide frequency for Enterococcus phage phiEF17H

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.168AlaAla: 0.168 ± 0.077
0.311AlaCys: 0.311 ± 0.098
3.279AlaAsp: 3.279 ± 0.341
4.356AlaGlu: 4.356 ± 0.345
2.202AlaPhe: 2.202 ± 0.215
3.112AlaGly: 3.112 ± 0.388
0.694AlaHis: 0.694 ± 0.128
3.878AlaIle: 3.878 ± 0.325
5.314AlaLys: 5.314 ± 0.381
5.218AlaLeu: 5.218 ± 0.389
1.556AlaMet: 1.556 ± 0.226
3.399AlaAsn: 3.399 ± 0.329
1.963AlaPro: 1.963 ± 0.237
2.154AlaGln: 2.154 ± 0.29
2.25AlaArg: 2.25 ± 0.241
3.327AlaSer: 3.327 ± 0.346
4.165AlaThr: 4.165 ± 0.339
4.069AlaVal: 4.069 ± 0.355
0.646AlaTrp: 0.646 ± 0.112
2.346AlaTyr: 2.346 ± 0.299
0.0AlaXaa: 0.0 ± 0.0
Cys
0.359CysAla: 0.359 ± 0.11
0.072CysCys: 0.072 ± 0.039
0.551CysAsp: 0.551 ± 0.12
0.574CysGlu: 0.574 ± 0.116
0.335CysPhe: 0.335 ± 0.093
0.838CysGly: 0.838 ± 0.175
0.072CysHis: 0.072 ± 0.04
0.335CysIle: 0.335 ± 0.09
0.551CysLys: 0.551 ± 0.131
0.838CysLeu: 0.838 ± 0.143
0.096CysMet: 0.096 ± 0.047
0.383CysAsn: 0.383 ± 0.113
0.431CysPro: 0.431 ± 0.121
0.168CysGln: 0.168 ± 0.056
0.407CysArg: 0.407 ± 0.098
0.503CysSer: 0.503 ± 0.109
0.503CysThr: 0.503 ± 0.124
0.551CysVal: 0.551 ± 0.124
0.072CysTrp: 0.072 ± 0.044
0.527CysTyr: 0.527 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
3.207AspAla: 3.207 ± 0.368
0.551AspCys: 0.551 ± 0.111
2.705AspAsp: 2.705 ± 0.299
5.385AspGlu: 5.385 ± 0.408
2.609AspPhe: 2.609 ± 0.219
3.71AspGly: 3.71 ± 0.318
0.598AspHis: 0.598 ± 0.11
4.859AspIle: 4.859 ± 0.299
5.457AspLys: 5.457 ± 0.415
5.314AspLeu: 5.314 ± 0.292
2.202AspMet: 2.202 ± 0.238
3.758AspAsn: 3.758 ± 0.305
1.388AspPro: 1.388 ± 0.204
1.053AspGln: 1.053 ± 0.169
2.154AspArg: 2.154 ± 0.245
3.854AspSer: 3.854 ± 0.345
3.878AspThr: 3.878 ± 0.266
4.596AspVal: 4.596 ± 0.385
0.981AspTrp: 0.981 ± 0.155
3.566AspTyr: 3.566 ± 0.271
0.0AspXaa: 0.0 ± 0.0
Glu
5.003GluAla: 5.003 ± 0.375
0.67GluCys: 0.67 ± 0.139
4.931GluAsp: 4.931 ± 0.37
9.119GluGlu: 9.119 ± 0.938
2.753GluPhe: 2.753 ± 0.247
4.835GluGly: 4.835 ± 0.348
1.556GluHis: 1.556 ± 0.24
5.146GluIle: 5.146 ± 0.393
6.439GluLys: 6.439 ± 0.479
8.952GluLeu: 8.952 ± 0.528
1.699GluMet: 1.699 ± 0.209
4.715GluAsn: 4.715 ± 0.318
2.585GluPro: 2.585 ± 0.264
4.021GluGln: 4.021 ± 0.35
3.327GluArg: 3.327 ± 0.321
4.38GluSer: 4.38 ± 0.343
4.261GluThr: 4.261 ± 0.34
6.415GluVal: 6.415 ± 0.466
1.101GluTrp: 1.101 ± 0.15
3.973GluTyr: 3.973 ± 0.311
0.0GluXaa: 0.0 ± 0.0
Phe
1.819PheAla: 1.819 ± 0.228
0.335PheCys: 0.335 ± 0.084
2.489PheAsp: 2.489 ± 0.275
2.489PheGlu: 2.489 ± 0.255
1.221PhePhe: 1.221 ± 0.178
2.25PheGly: 2.25 ± 0.278
0.574PheHis: 0.574 ± 0.145
2.896PheIle: 2.896 ± 0.311
2.705PheLys: 2.705 ± 0.244
3.136PheLeu: 3.136 ± 0.315
0.933PheMet: 0.933 ± 0.127
2.609PheAsn: 2.609 ± 0.268
1.029PhePro: 1.029 ± 0.153
1.077PheGln: 1.077 ± 0.171
1.436PheArg: 1.436 ± 0.179
3.112PheSer: 3.112 ± 0.226
2.8PheThr: 2.8 ± 0.3
2.226PheVal: 2.226 ± 0.21
0.335PheTrp: 0.335 ± 0.104
1.795PheTyr: 1.795 ± 0.206
0.0PheXaa: 0.0 ± 0.0
Gly
3.638GlyAla: 3.638 ± 0.495
0.527GlyCys: 0.527 ± 0.119
3.901GlyAsp: 3.901 ± 0.339
4.907GlyGlu: 4.907 ± 0.326
2.298GlyPhe: 2.298 ± 0.222
4.835GlyGly: 4.835 ± 0.58
0.957GlyHis: 0.957 ± 0.15
4.093GlyIle: 4.093 ± 0.319
5.385GlyLys: 5.385 ± 0.362
4.787GlyLeu: 4.787 ± 0.353
1.771GlyMet: 1.771 ± 0.251
3.279GlyAsn: 3.279 ± 0.264
0.048GlyPro: 0.048 ± 0.037
1.819GlyGln: 1.819 ± 0.236
2.633GlyArg: 2.633 ± 0.284
4.117GlySer: 4.117 ± 0.495
4.643GlyThr: 4.643 ± 0.367
4.332GlyVal: 4.332 ± 0.301
1.029GlyTrp: 1.029 ± 0.172
3.901GlyTyr: 3.901 ± 0.378
0.0GlyXaa: 0.0 ± 0.0
His
0.79HisAla: 0.79 ± 0.137
0.263HisCys: 0.263 ± 0.086
0.933HisAsp: 0.933 ± 0.138
0.933HisGlu: 0.933 ± 0.14
0.527HisPhe: 0.527 ± 0.118
1.005HisGly: 1.005 ± 0.175
0.287HisHis: 0.287 ± 0.087
1.077HisIle: 1.077 ± 0.204
1.293HisLys: 1.293 ± 0.188
1.125HisLeu: 1.125 ± 0.204
0.263HisMet: 0.263 ± 0.07
0.933HisAsn: 0.933 ± 0.168
0.574HisPro: 0.574 ± 0.104
0.407HisGln: 0.407 ± 0.091
0.574HisArg: 0.574 ± 0.107
0.814HisSer: 0.814 ± 0.138
1.173HisThr: 1.173 ± 0.184
1.197HisVal: 1.197 ± 0.185
0.239HisTrp: 0.239 ± 0.081
0.838HisTyr: 0.838 ± 0.137
0.0HisXaa: 0.0 ± 0.0
Ile
3.83IleAla: 3.83 ± 0.327
0.407IleCys: 0.407 ± 0.1
5.003IleAsp: 5.003 ± 0.306
6.056IleGlu: 6.056 ± 0.403
1.987IlePhe: 1.987 ± 0.204
3.447IleGly: 3.447 ± 0.387
1.149IleHis: 1.149 ± 0.19
4.62IleIle: 4.62 ± 0.378
5.649IleLys: 5.649 ± 0.34
4.308IleLeu: 4.308 ± 0.356
1.556IleMet: 1.556 ± 0.202
4.237IleAsn: 4.237 ± 0.331
2.13IlePro: 2.13 ± 0.244
2.226IleGln: 2.226 ± 0.285
2.777IleArg: 2.777 ± 0.258
4.596IleSer: 4.596 ± 0.317
3.758IleThr: 3.758 ± 0.352
3.686IleVal: 3.686 ± 0.354
0.431IleTrp: 0.431 ± 0.099
2.346IleTyr: 2.346 ± 0.224
0.0IleXaa: 0.0 ± 0.0
Lys
4.404LysAla: 4.404 ± 0.343
0.766LysCys: 0.766 ± 0.169
5.218LysAsp: 5.218 ± 0.407
8.976LysGlu: 8.976 ± 0.609
2.394LysPhe: 2.394 ± 0.268
4.5LysGly: 4.5 ± 0.322
1.293LysHis: 1.293 ± 0.174
4.141LysIle: 4.141 ± 0.297
6.08LysLys: 6.08 ± 0.476
6.917LysLeu: 6.917 ± 0.419
2.106LysMet: 2.106 ± 0.251
4.428LysAsn: 4.428 ± 0.248
2.92LysPro: 2.92 ± 0.266
2.992LysGln: 2.992 ± 0.301
3.686LysArg: 3.686 ± 0.34
3.878LysSer: 3.878 ± 0.344
4.524LysThr: 4.524 ± 0.288
6.127LysVal: 6.127 ± 0.406
0.718LysTrp: 0.718 ± 0.14
3.112LysTyr: 3.112 ± 0.266
0.0LysXaa: 0.0 ± 0.0
Leu
5.457LeuAla: 5.457 ± 0.354
0.551LeuCys: 0.551 ± 0.127
6.104LeuAsp: 6.104 ± 0.389
7.851LeuGlu: 7.851 ± 0.48
2.992LeuPhe: 2.992 ± 0.325
6.271LeuGly: 6.271 ± 0.484
1.029LeuHis: 1.029 ± 0.156
4.835LeuIle: 4.835 ± 0.342
6.295LeuLys: 6.295 ± 0.353
7.731LeuLeu: 7.731 ± 0.589
2.322LeuMet: 2.322 ± 0.216
5.721LeuAsn: 5.721 ± 0.501
2.753LeuPro: 2.753 ± 0.281
3.303LeuGln: 3.303 ± 0.264
3.686LeuArg: 3.686 ± 0.261
5.673LeuSer: 5.673 ± 0.38
5.649LeuThr: 5.649 ± 0.473
4.284LeuVal: 4.284 ± 0.332
0.694LeuTrp: 0.694 ± 0.131
3.279LeuTyr: 3.279 ± 0.354
0.0LeuXaa: 0.0 ± 0.0
Met
1.675MetAla: 1.675 ± 0.186
0.168MetCys: 0.168 ± 0.058
1.412MetAsp: 1.412 ± 0.168
2.058MetGlu: 2.058 ± 0.226
0.838MetPhe: 0.838 ± 0.145
1.484MetGly: 1.484 ± 0.255
0.455MetHis: 0.455 ± 0.099
1.46MetIle: 1.46 ± 0.2
2.298MetLys: 2.298 ± 0.257
2.322MetLeu: 2.322 ± 0.255
0.455MetMet: 0.455 ± 0.089
1.747MetAsn: 1.747 ± 0.181
0.67MetPro: 0.67 ± 0.121
0.814MetGln: 0.814 ± 0.186
1.125MetArg: 1.125 ± 0.172
1.867MetSer: 1.867 ± 0.197
1.604MetThr: 1.604 ± 0.184
1.364MetVal: 1.364 ± 0.19
0.168MetTrp: 0.168 ± 0.066
1.053MetTyr: 1.053 ± 0.173
0.0MetXaa: 0.0 ± 0.0
Asn
2.824AsnAla: 2.824 ± 0.275
0.431AsnCys: 0.431 ± 0.111
3.136AsnAsp: 3.136 ± 0.279
4.356AsnGlu: 4.356 ± 0.328
1.891AsnPhe: 1.891 ± 0.251
4.979AsnGly: 4.979 ± 0.405
1.34AsnHis: 1.34 ± 0.209
3.662AsnIle: 3.662 ± 0.305
5.553AsnLys: 5.553 ± 0.372
4.476AsnLeu: 4.476 ± 0.291
1.508AsnMet: 1.508 ± 0.206
4.069AsnAsn: 4.069 ± 0.379
2.058AsnPro: 2.058 ± 0.207
2.13AsnGln: 2.13 ± 0.298
2.561AsnArg: 2.561 ± 0.266
3.854AsnSer: 3.854 ± 0.329
3.614AsnThr: 3.614 ± 0.283
3.686AsnVal: 3.686 ± 0.285
0.574AsnTrp: 0.574 ± 0.103
2.729AsnTyr: 2.729 ± 0.233
0.0AsnXaa: 0.0 ± 0.0
Pro
1.508ProAla: 1.508 ± 0.214
0.311ProCys: 0.311 ± 0.079
2.25ProAsp: 2.25 ± 0.248
2.968ProGlu: 2.968 ± 0.284
1.293ProPhe: 1.293 ± 0.176
0.503ProGly: 0.503 ± 0.116
0.527ProHis: 0.527 ± 0.103
2.082ProIle: 2.082 ± 0.268
2.25ProLys: 2.25 ± 0.28
2.513ProLeu: 2.513 ± 0.271
0.598ProMet: 0.598 ± 0.132
1.987ProAsn: 1.987 ± 0.234
0.527ProPro: 0.527 ± 0.118
1.053ProGln: 1.053 ± 0.256
1.221ProArg: 1.221 ± 0.174
2.106ProSer: 2.106 ± 0.266
2.346ProThr: 2.346 ± 0.205
2.561ProVal: 2.561 ± 0.328
0.287ProTrp: 0.287 ± 0.084
1.652ProTyr: 1.652 ± 0.198
0.0ProXaa: 0.0 ± 0.0
Gln
2.585GlnAla: 2.585 ± 0.268
0.144GlnCys: 0.144 ± 0.06
1.412GlnAsp: 1.412 ± 0.17
2.92GlnGlu: 2.92 ± 0.325
1.508GlnPhe: 1.508 ± 0.205
2.178GlnGly: 2.178 ± 0.258
0.359GlnHis: 0.359 ± 0.105
1.891GlnIle: 1.891 ± 0.19
2.106GlnLys: 2.106 ± 0.245
3.279GlnLeu: 3.279 ± 0.261
0.79GlnMet: 0.79 ± 0.149
1.388GlnAsn: 1.388 ± 0.218
1.628GlnPro: 1.628 ± 0.401
1.915GlnGln: 1.915 ± 0.459
1.628GlnArg: 1.628 ± 0.199
1.843GlnSer: 1.843 ± 0.24
1.604GlnThr: 1.604 ± 0.174
2.777GlnVal: 2.777 ± 0.288
0.383GlnTrp: 0.383 ± 0.101
1.652GlnTyr: 1.652 ± 0.208
0.0GlnXaa: 0.0 ± 0.0
Arg
2.417ArgAla: 2.417 ± 0.35
0.407ArgCys: 0.407 ± 0.123
2.346ArgAsp: 2.346 ± 0.279
3.638ArgGlu: 3.638 ± 0.312
1.675ArgPhe: 1.675 ± 0.236
2.609ArgGly: 2.609 ± 0.248
0.694ArgHis: 0.694 ± 0.149
2.633ArgIle: 2.633 ± 0.296
3.159ArgLys: 3.159 ± 0.269
3.83ArgLeu: 3.83 ± 0.289
1.029ArgMet: 1.029 ± 0.16
2.226ArgAsn: 2.226 ± 0.228
1.005ArgPro: 1.005 ± 0.184
1.46ArgGln: 1.46 ± 0.219
1.628ArgArg: 1.628 ± 0.194
2.011ArgSer: 2.011 ± 0.188
2.465ArgThr: 2.465 ± 0.239
3.447ArgVal: 3.447 ± 0.28
0.311ArgTrp: 0.311 ± 0.087
2.058ArgTyr: 2.058 ± 0.274
0.0ArgXaa: 0.0 ± 0.0
Ser
3.136SerAla: 3.136 ± 0.342
0.503SerCys: 0.503 ± 0.135
4.069SerAsp: 4.069 ± 0.382
4.045SerGlu: 4.045 ± 0.321
2.753SerPhe: 2.753 ± 0.284
4.237SerGly: 4.237 ± 0.391
0.766SerHis: 0.766 ± 0.148
4.452SerIle: 4.452 ± 0.294
5.17SerLys: 5.17 ± 0.452
5.553SerLeu: 5.553 ± 0.332
1.867SerMet: 1.867 ± 0.183
3.112SerAsn: 3.112 ± 0.27
1.652SerPro: 1.652 ± 0.203
2.202SerGln: 2.202 ± 0.237
2.322SerArg: 2.322 ± 0.272
4.356SerSer: 4.356 ± 0.352
3.997SerThr: 3.997 ± 0.452
4.237SerVal: 4.237 ± 0.336
0.742SerTrp: 0.742 ± 0.12
2.848SerTyr: 2.848 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
3.949ThrAla: 3.949 ± 0.35
0.455ThrCys: 0.455 ± 0.121
3.83ThrAsp: 3.83 ± 0.324
4.715ThrGlu: 4.715 ± 0.447
3.016ThrPhe: 3.016 ± 0.277
4.045ThrGly: 4.045 ± 0.396
0.886ThrHis: 0.886 ± 0.142
4.643ThrIle: 4.643 ± 0.417
4.643ThrLys: 4.643 ± 0.343
5.816ThrLeu: 5.816 ± 0.341
1.173ThrMet: 1.173 ± 0.17
3.375ThrAsn: 3.375 ± 0.289
2.537ThrPro: 2.537 ± 0.249
1.771ThrGln: 1.771 ± 0.196
2.585ThrArg: 2.585 ± 0.244
3.949ThrSer: 3.949 ± 0.343
4.213ThrThr: 4.213 ± 0.405
4.859ThrVal: 4.859 ± 0.503
0.742ThrTrp: 0.742 ± 0.135
3.136ThrTyr: 3.136 ± 0.264
0.0ThrXaa: 0.0 ± 0.0
Val
4.38ValAla: 4.38 ± 0.327
0.646ValCys: 0.646 ± 0.129
4.763ValAsp: 4.763 ± 0.376
5.864ValGlu: 5.864 ± 0.48
3.016ValPhe: 3.016 ± 0.324
4.069ValGly: 4.069 ± 0.336
1.125ValHis: 1.125 ± 0.17
4.189ValIle: 4.189 ± 0.322
4.476ValLys: 4.476 ± 0.418
5.266ValLeu: 5.266 ± 0.394
1.771ValMet: 1.771 ± 0.195
4.308ValAsn: 4.308 ± 0.354
2.681ValPro: 2.681 ± 0.253
1.699ValGln: 1.699 ± 0.23
2.896ValArg: 2.896 ± 0.306
4.548ValSer: 4.548 ± 0.325
5.218ValThr: 5.218 ± 0.446
5.146ValVal: 5.146 ± 0.406
0.718ValTrp: 0.718 ± 0.145
3.183ValTyr: 3.183 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
0.718TrpAla: 0.718 ± 0.139
0.168TrpCys: 0.168 ± 0.068
0.527TrpAsp: 0.527 ± 0.117
1.053TrpGlu: 1.053 ± 0.161
0.479TrpPhe: 0.479 ± 0.098
0.814TrpGly: 0.814 ± 0.131
0.144TrpHis: 0.144 ± 0.054
0.455TrpIle: 0.455 ± 0.107
0.67TrpLys: 0.67 ± 0.126
1.149TrpLeu: 1.149 ± 0.161
0.12TrpMet: 0.12 ± 0.053
0.718TrpAsn: 0.718 ± 0.132
0.0TrpPro: 0.0 ± 0.0
0.407TrpGln: 0.407 ± 0.108
0.407TrpArg: 0.407 ± 0.095
0.455TrpSer: 0.455 ± 0.115
0.91TrpThr: 0.91 ± 0.184
0.766TrpVal: 0.766 ± 0.17
0.239TrpTrp: 0.239 ± 0.087
0.79TrpTyr: 0.79 ± 0.158
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.537TyrAla: 2.537 ± 0.302
0.479TyrCys: 0.479 ± 0.104
2.968TyrAsp: 2.968 ± 0.276
3.614TyrGlu: 3.614 ± 0.338
1.484TyrPhe: 1.484 ± 0.202
3.183TyrGly: 3.183 ± 0.322
0.742TyrHis: 0.742 ± 0.141
2.848TyrIle: 2.848 ± 0.299
3.686TyrLys: 3.686 ± 0.262
3.949TyrLeu: 3.949 ± 0.349
1.197TyrMet: 1.197 ± 0.18
3.04TyrAsn: 3.04 ± 0.324
1.963TyrPro: 1.963 ± 0.243
1.316TyrGln: 1.316 ± 0.206
1.771TyrArg: 1.771 ± 0.234
2.777TyrSer: 2.777 ± 0.244
3.016TyrThr: 3.016 ± 0.312
3.638TyrVal: 3.638 ± 0.299
0.574TyrTrp: 0.574 ± 0.133
2.322TyrTyr: 2.322 ± 0.296
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 204 proteins (41780 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski