Hamiltosporidium magnivora

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Pansporoblastina; Dubosqiidae; Hamiltosporidium

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4459 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q9LGS8|A0A4Q9LGS8_9MICR SWI/SNF2-like chromatin-remodeling complex ATPase OS=Hamiltosporidium magnivora OX=148818 GN=CWI36_0319p0020 PE=4 SV=1
MM1 pKa = 7.18TMIIIVVTNGLNGDD15 pKa = 4.14DD16 pKa = 3.8YY17 pKa = 11.9LLFGMWKK24 pKa = 10.29VKK26 pKa = 9.5MSMMANEE33 pKa = 4.81SNDD36 pKa = 3.32KK37 pKa = 10.83DD38 pKa = 3.64NHH40 pKa = 5.57EE41 pKa = 4.62SCDD44 pKa = 3.52GDD46 pKa = 3.92VGVGDD51 pKa = 5.22VDD53 pKa = 4.09GCDD56 pKa = 4.79DD57 pKa = 5.09KK58 pKa = 11.82DD59 pKa = 4.07DD60 pKa = 4.64HH61 pKa = 7.27DD62 pKa = 4.44SCEE65 pKa = 4.16GATEE69 pKa = 4.67GEE71 pKa = 4.34DD72 pKa = 3.39EE73 pKa = 5.49SDD75 pKa = 3.23IVWYY79 pKa = 9.02TEE81 pKa = 4.53YY82 pKa = 11.32DD83 pKa = 3.95DD84 pKa = 6.18DD85 pKa = 5.7DD86 pKa = 5.32CGYY89 pKa = 11.28DD90 pKa = 3.52DD91 pKa = 6.04GNVEE95 pKa = 4.48GDD97 pKa = 4.09DD98 pKa = 4.38HH99 pKa = 7.45DD100 pKa = 4.68SCDD103 pKa = 5.06GEE105 pKa = 5.5GEE107 pKa = 4.52DD108 pKa = 5.03DD109 pKa = 3.62TDD111 pKa = 3.37GTRR114 pKa = 11.84DD115 pKa = 3.24IKK117 pKa = 11.14YY118 pKa = 10.83DD119 pKa = 3.64SDD121 pKa = 2.88NWGYY125 pKa = 11.33DD126 pKa = 3.05EE127 pKa = 6.4GSDD130 pKa = 5.22DD131 pKa = 4.97RR132 pKa = 11.84DD133 pKa = 3.61DD134 pKa = 5.61HH135 pKa = 6.55EE136 pKa = 5.29SCDD139 pKa = 3.78GATEE143 pKa = 4.27GVDD146 pKa = 3.17GSEE149 pKa = 4.2GIEE152 pKa = 3.76RR153 pKa = 11.84TEE155 pKa = 5.11RR156 pKa = 11.84DD157 pKa = 3.56NDD159 pKa = 3.46DD160 pKa = 4.13CAYY163 pKa = 10.48EE164 pKa = 4.86DD165 pKa = 5.61DD166 pKa = 5.45NDD168 pKa = 5.41DD169 pKa = 4.56GDD171 pKa = 4.54DD172 pKa = 4.04QEE174 pKa = 5.35SCDD177 pKa = 3.7GFGDD181 pKa = 3.65EE182 pKa = 5.18VYY184 pKa = 9.03TLEE187 pKa = 5.41VIFEE191 pKa = 4.18SDD193 pKa = 4.36DD194 pKa = 4.21DD195 pKa = 4.18DD196 pKa = 4.15VVYY199 pKa = 11.41NNGEE203 pKa = 4.33DD204 pKa = 3.25NWEE207 pKa = 4.08GSVVCNDD214 pKa = 3.17KK215 pKa = 11.37KK216 pKa = 10.99EE217 pKa = 4.39DD218 pKa = 3.68GVGIDD223 pKa = 4.48GNDD226 pKa = 3.37SGEE229 pKa = 4.13GNIEE233 pKa = 4.24NISEE237 pKa = 4.24GEE239 pKa = 3.79IGEE242 pKa = 4.19QEE244 pKa = 4.18DD245 pKa = 4.24GKK247 pKa = 10.3VQHH250 pKa = 6.81EE251 pKa = 4.61GTVVIRR257 pKa = 11.84EE258 pKa = 4.05RR259 pKa = 11.84GGDD262 pKa = 3.62RR263 pKa = 11.84FWYY266 pKa = 10.54GDD268 pKa = 3.4FGSDD272 pKa = 2.46EE273 pKa = 4.43KK274 pKa = 11.68YY275 pKa = 10.97SVFEE279 pKa = 4.54DD280 pKa = 3.52LRR282 pKa = 11.84QEE284 pKa = 3.78EE285 pKa = 5.12DD286 pKa = 2.71IWDD289 pKa = 3.83VAEE292 pKa = 5.03LLLKK296 pKa = 10.83KK297 pKa = 10.68LGFAVNYY304 pKa = 10.53AYY306 pKa = 10.34GKK308 pKa = 8.96KK309 pKa = 10.36LKK311 pKa = 10.65VLMVGIGGNSGVVSIKK327 pKa = 9.54TEE329 pKa = 3.91EE330 pKa = 3.82YY331 pKa = 10.4KK332 pKa = 10.92YY333 pKa = 11.1LRR335 pKa = 11.84DD336 pKa = 3.77CDD338 pKa = 3.55ILEE341 pKa = 5.64DD342 pKa = 3.11IWEE345 pKa = 4.63DD346 pKa = 3.75FNSEE350 pKa = 4.57RR351 pKa = 11.84VSDD354 pKa = 4.14CNNGVFEE361 pKa = 4.92NKK363 pKa = 9.96NSGNFEE369 pKa = 4.16TSDD372 pKa = 3.4EE373 pKa = 4.49RR374 pKa = 11.84GDD376 pKa = 3.78GFDD379 pKa = 6.06KK380 pKa = 10.45IDD382 pKa = 3.73EE383 pKa = 4.75DD384 pKa = 4.43GKK386 pKa = 10.85CGKK389 pKa = 10.1GSKK392 pKa = 10.14DD393 pKa = 3.95CEE395 pKa = 4.14DD396 pKa = 3.85CKK398 pKa = 11.13GDD400 pKa = 3.62EE401 pKa = 4.15VDD403 pKa = 3.68KK404 pKa = 11.38HH405 pKa = 7.22GEE407 pKa = 3.81TRR409 pKa = 11.84KK410 pKa = 10.02CGKK413 pKa = 9.35VGKK416 pKa = 9.58FGKK419 pKa = 10.04VVEE422 pKa = 4.15RR423 pKa = 11.84LEE425 pKa = 5.44RR426 pKa = 11.84DD427 pKa = 3.47DD428 pKa = 6.54LKK430 pKa = 11.55DD431 pKa = 3.23NWVEE435 pKa = 4.62CGNCGNSVNWINCGEE450 pKa = 4.15WGNGLIVVIGFVEE463 pKa = 4.75IIVLIEE469 pKa = 3.69WSLKK473 pKa = 9.27IGLVIEE479 pKa = 5.12GIGLADD485 pKa = 4.05LLVAIEE491 pKa = 4.72AFDD494 pKa = 3.83LVEE497 pKa = 4.83PIVLFVVIVLVEE509 pKa = 4.31PIVLFEE515 pKa = 3.9VSMLVEE521 pKa = 4.74IIEE524 pKa = 4.57IIGLIVVIMIEE535 pKa = 4.46MIEE538 pKa = 4.27MIEE541 pKa = 4.21VIVVIVLVEE550 pKa = 4.61PIDD553 pKa = 3.91LVLVGVLVAMIVVIMNLVVFNCNDD577 pKa = 2.85VCGFGGKK584 pKa = 9.34SDD586 pKa = 3.72GVSNDD591 pKa = 2.96MSDD594 pKa = 4.33SKK596 pKa = 11.79DD597 pKa = 3.45CGISGEE603 pKa = 4.19LCFVKK608 pKa = 10.5VGKK611 pKa = 10.35CDD613 pKa = 3.29EE614 pKa = 4.54CVNCAEE620 pKa = 4.66CNGIFEE626 pKa = 4.78GVCDD630 pKa = 4.45CDD632 pKa = 4.15VEE634 pKa = 4.48CTYY637 pKa = 11.2RR638 pKa = 11.84KK639 pKa = 10.01LDD641 pKa = 3.96LYY643 pKa = 10.38CGKK646 pKa = 10.61RR647 pKa = 11.84GGHH650 pKa = 5.84CSKK653 pKa = 11.26CEE655 pKa = 3.97GFDD658 pKa = 4.22GKK660 pKa = 10.72CGCNGNSGKK669 pKa = 10.03IGRR672 pKa = 11.84FVTCGEE678 pKa = 3.87LDD680 pKa = 3.6GRR682 pKa = 11.84CDD684 pKa = 4.67SGSCDD689 pKa = 3.23NCNDD693 pKa = 3.28FPEE696 pKa = 4.65TFGEE700 pKa = 4.29CSEE703 pKa = 4.39SCEE706 pKa = 4.28EE707 pKa = 3.99ASGMDD712 pKa = 4.32TLSAWNTVILFASPGSDD729 pKa = 2.74VVTFAPVGGIGGLGFGLFVGGLFVGGVFVGGVFAGGVFAGGVFAGGVFGGGVFAGGVFVGGVFVGGVFVGGVFAGGVFVGGVFVGGVFVGGVFVGGVFVGGVFVGGVFVGGVFVGGVFVGGVV851 pKa = 2.97

Molecular weight: 90.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q9LMK5|A0A4Q9LMK5_9MICR Uncharacterized protein (Fragment) OS=Hamiltosporidium magnivora OX=148818 GN=CWI36_0022p0050 PE=4 SV=1
MM1 pKa = 7.84IILNILSKK9 pKa = 10.43ISRR12 pKa = 11.84VCASFSSRR20 pKa = 11.84SYY22 pKa = 10.08KK23 pKa = 10.38SSSSRR28 pKa = 11.84VSSHH32 pKa = 6.49SGGGSGKK39 pKa = 8.88TSLSHH44 pKa = 6.51SGYY47 pKa = 9.64SSSGGGSDD55 pKa = 3.68GSSSSRR61 pKa = 11.84GSSSGGSSSSGGGSGGSSSSSGSSSSGGSSSSGGSSGSGGSSGSGGGSGSGGGSGSRR118 pKa = 11.84GGSGSRR124 pKa = 11.84GGSGSGGGSGSGGGSGGSTGSSSSQGSSNGKK155 pKa = 9.17CCPCPSS161 pKa = 3.45

Molecular weight: 13.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4459

0

4459

1572801

41

2447

352.7

41.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.474 ± 0.028

1.959 ± 0.018

5.207 ± 0.026

7.493 ± 0.04

6.36 ± 0.043

3.504 ± 0.043

1.651 ± 0.015

9.96 ± 0.037

9.942 ± 0.05

9.582 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.94 ± 0.014

8.785 ± 0.047

2.536 ± 0.025

2.487 ± 0.019

3.534 ± 0.025

7.864 ± 0.031

4.988 ± 0.035

4.596 ± 0.03

0.489 ± 0.007

4.648 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski