Verticiella sediminum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Verticiella

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5320 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A556AGL5|A0A556AGL5_9BURK Tripartite tricarboxylate transporter substrate binding protein OS=Verticiella sediminum OX=1247510 GN=FOZ76_17550 PE=3 SV=1
MM1 pKa = 7.72AISIPEE7 pKa = 3.74ARR9 pKa = 11.84EE10 pKa = 3.65AVAGLFISYY19 pKa = 10.18LGRR22 pKa = 11.84APEE25 pKa = 4.06YY26 pKa = 10.7AAMSYY31 pKa = 10.79YY32 pKa = 10.49VGRR35 pKa = 11.84LQAILGGQEE44 pKa = 4.05EE45 pKa = 5.34EE46 pKa = 4.36DD47 pKa = 3.68TEE49 pKa = 4.65SAVKK53 pKa = 10.37ALSAEE58 pKa = 4.52IYY60 pKa = 8.29QTAGRR65 pKa = 11.84VGEE68 pKa = 4.35IPSGANVTHH77 pKa = 6.91GEE79 pKa = 4.05LVDD82 pKa = 3.4WVYY85 pKa = 11.68QNILGRR91 pKa = 11.84AADD94 pKa = 3.75AEE96 pKa = 4.27GRR98 pKa = 11.84GYY100 pKa = 10.08WISQLEE106 pKa = 4.26SGNFGPQDD114 pKa = 3.47LVATVIAAVQGQGEE128 pKa = 4.55GRR130 pKa = 11.84DD131 pKa = 3.34FDD133 pKa = 4.16YY134 pKa = 10.97FSNRR138 pKa = 11.84VEE140 pKa = 4.21VALEE144 pKa = 3.63FAKK147 pKa = 10.78FGNSNPNVLAKK158 pKa = 10.74LPFDD162 pKa = 4.22AAQVLAGVNEE172 pKa = 4.44DD173 pKa = 3.72SATVDD178 pKa = 3.05AAFDD182 pKa = 3.4KK183 pKa = 11.04LYY185 pKa = 10.63AARR188 pKa = 11.84HH189 pKa = 5.25GGEE192 pKa = 4.34TVYY195 pKa = 9.56LTPGVDD201 pKa = 3.44TLEE204 pKa = 4.52GTSGDD209 pKa = 3.75DD210 pKa = 3.43TFVSLPVNPATGQEE224 pKa = 4.31TSTLTAFDD232 pKa = 5.05SIDD235 pKa = 3.41GGAGKK240 pKa = 8.54DD241 pKa = 3.1TLQIHH246 pKa = 6.08FVQGTNDD253 pKa = 3.67TLPANLAVRR262 pKa = 11.84NVEE265 pKa = 4.16VIRR268 pKa = 11.84LVSEE272 pKa = 4.61DD273 pKa = 3.1GSGFGEE279 pKa = 4.56SIDD282 pKa = 3.52AARR285 pKa = 11.84FAGAQEE291 pKa = 3.76IWQVGSEE298 pKa = 3.8ATIYY302 pKa = 10.87NVGAGMTAGFAGFSDD317 pKa = 3.83EE318 pKa = 4.99NIDD321 pKa = 3.61VFLADD326 pKa = 4.24GVGTANIRR334 pKa = 11.84LEE336 pKa = 4.69GIGPDD341 pKa = 3.62DD342 pKa = 4.92AFDD345 pKa = 4.08PEE347 pKa = 3.89IDD349 pKa = 3.26IDD351 pKa = 4.4VEE353 pKa = 4.75GKK355 pKa = 10.51ALGTVNLSGTVVGDD369 pKa = 3.43DD370 pKa = 3.9TYY372 pKa = 11.77VHH374 pKa = 6.94LDD376 pKa = 3.29AYY378 pKa = 11.06VGTKK382 pKa = 9.86VQTFTLNTEE391 pKa = 3.9IATLLHH397 pKa = 6.42IYY399 pKa = 10.58GYY401 pKa = 10.71GDD403 pKa = 2.83TDD405 pKa = 3.79AAVKK409 pKa = 9.44TVDD412 pKa = 3.43ASASSGAILYY422 pKa = 9.15EE423 pKa = 3.86VDD425 pKa = 3.63EE426 pKa = 4.77SVSSVKK432 pKa = 9.92TGSGDD437 pKa = 3.58DD438 pKa = 3.63LVVFDD443 pKa = 4.31SGRR446 pKa = 11.84GIRR449 pKa = 11.84AGDD452 pKa = 3.53TVDD455 pKa = 3.83AGEE458 pKa = 4.84GEE460 pKa = 4.17NAVAFLQSTFQTEE473 pKa = 4.05NYY475 pKa = 9.99DD476 pKa = 4.13AINSGLANVQTLIFAGEE493 pKa = 4.18DD494 pKa = 3.46VAFDD498 pKa = 3.58ASKK501 pKa = 9.94VANYY505 pKa = 7.4KK506 pKa = 9.12TLVVADD512 pKa = 4.11AEE514 pKa = 4.77GDD516 pKa = 3.69GSQATVSNLAADD528 pKa = 3.59QTLVLVEE535 pKa = 3.92NDD537 pKa = 3.35GVVFDD542 pKa = 5.17LDD544 pKa = 3.63AVQGVQLLNAAADD557 pKa = 3.95VNVEE561 pKa = 4.05VAFSKK566 pKa = 10.82FSYY569 pKa = 10.46VYY571 pKa = 10.61LAFGEE576 pKa = 4.62DD577 pKa = 4.21GEE579 pKa = 4.86SLGATGGTLALTGTAWAPEE598 pKa = 3.84ISVFAPGGNLSFSNEE613 pKa = 3.82SGKK616 pKa = 10.54FSTIDD621 pKa = 3.25ASALEE626 pKa = 4.02ASLYY630 pKa = 9.56IGGMSSAVAEE640 pKa = 4.1TLILGEE646 pKa = 4.61GVDD649 pKa = 4.41AMIHH653 pKa = 6.01LSVGTAAGEE662 pKa = 4.05NSSSIGALDD671 pKa = 3.91VIEE674 pKa = 5.28GYY676 pKa = 10.05NAEE679 pKa = 4.4TGHH682 pKa = 6.29LWGVNAVDD690 pKa = 3.92TLVLDD695 pKa = 4.02SSVDD699 pKa = 3.81SLLKK703 pKa = 10.38AYY705 pKa = 10.26SAAAVFYY712 pKa = 11.02NAGEE716 pKa = 4.09YY717 pKa = 10.14DD718 pKa = 3.42AEE720 pKa = 4.43ANQVVLFVYY729 pKa = 10.59GGDD732 pKa = 3.37TYY734 pKa = 11.48LYY736 pKa = 10.42ADD738 pKa = 4.54TYY740 pKa = 10.3TPDD743 pKa = 3.44GAAAGYY749 pKa = 8.7DD750 pKa = 3.47AQDD753 pKa = 3.38FAIKK757 pKa = 10.48LVGEE761 pKa = 4.13YY762 pKa = 10.25SQADD766 pKa = 3.74FAEE769 pKa = 4.64LGG771 pKa = 3.56

Molecular weight: 80.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A556AKP5|A0A556AKP5_9BURK BamA/TamA family outer membrane protein OS=Verticiella sediminum OX=1247510 GN=FOZ76_13985 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.19RR14 pKa = 11.84THH16 pKa = 5.62GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5320

0

5320

1790601

36

3446

336.6

36.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.652 ± 0.053

0.952 ± 0.011

5.349 ± 0.023

5.331 ± 0.026

3.258 ± 0.021

8.749 ± 0.034

2.3 ± 0.015

4.219 ± 0.025

2.244 ± 0.024

10.646 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.396 ± 0.014

2.3 ± 0.019

5.615 ± 0.025

3.707 ± 0.022

7.776 ± 0.036

5.031 ± 0.021

4.958 ± 0.022

7.784 ± 0.025

1.386 ± 0.013

2.345 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski