Mycobacterium phage LilMcDreamy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; unclassified Bclasvirinae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D994|A0A5P8D994_9CAUD Uncharacterized protein OS=Mycobacterium phage LilMcDreamy OX=2652422 GN=40 PE=4 SV=1
MM1 pKa = 6.79TQPDD5 pKa = 4.75AIQWGVRR12 pKa = 11.84QEE14 pKa = 4.0VPIIPDD20 pKa = 3.29GEE22 pKa = 4.22MPEE25 pKa = 4.5QIPAPAPIDD34 pKa = 3.6YY35 pKa = 9.98TGMTEE40 pKa = 4.03EE41 pKa = 4.14QVAEE45 pKa = 4.26AEE47 pKa = 4.25AQQGVDD53 pKa = 4.02FAAWNAVMAGWYY65 pKa = 9.66QDD67 pKa = 3.48VLALIEE73 pKa = 4.6AHH75 pKa = 6.32PEE77 pKa = 3.0WWQYY81 pKa = 7.43TVQVYY86 pKa = 10.2PDD88 pKa = 3.39EE89 pKa = 4.46ATARR93 pKa = 11.84EE94 pKa = 4.08QLALTRR100 pKa = 11.84EE101 pKa = 4.38ANQGNAFVRR110 pKa = 11.84SVDD113 pKa = 3.81LVTAPVITWTVVEE126 pKa = 4.32AA127 pKa = 4.24

Molecular weight: 14.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D7Q3|A0A5P8D7Q3_9CAUD Uncharacterized protein OS=Mycobacterium phage LilMcDreamy OX=2652422 GN=91 PE=4 SV=1
MM1 pKa = 7.59VMTQSAPQPGSRR13 pKa = 11.84RR14 pKa = 11.84STNKK18 pKa = 8.97TSNRR22 pKa = 11.84DD23 pKa = 2.92TRR25 pKa = 11.84ALLRR29 pKa = 11.84AIEE32 pKa = 4.08AAGGCLEE39 pKa = 4.16ACRR42 pKa = 11.84SRR44 pKa = 11.84SGHH47 pKa = 4.84YY48 pKa = 9.53KK49 pKa = 9.97VYY51 pKa = 11.02LEE53 pKa = 4.27GRR55 pKa = 11.84LIGTIAGTPSEE66 pKa = 4.13YY67 pKa = 9.91RR68 pKa = 11.84SRR70 pKa = 11.84KK71 pKa = 9.28NDD73 pKa = 3.04IANLRR78 pKa = 11.84RR79 pKa = 11.84NGLQITSKK87 pKa = 9.61GTYY90 pKa = 9.9APP92 pKa = 4.14

Molecular weight: 10.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

22959

30

2070

231.9

24.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.47 ± 0.372

0.963 ± 0.132

6.825 ± 0.248

5.719 ± 0.278

2.504 ± 0.098

9.561 ± 0.508

1.79 ± 0.136

4.438 ± 0.148

2.888 ± 0.161

7.892 ± 0.199

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.069 ± 0.104

2.883 ± 0.187

6.22 ± 0.205

3.149 ± 0.14

6.943 ± 0.338

4.974 ± 0.167

7.069 ± 0.239

7.509 ± 0.181

1.969 ± 0.14

2.165 ± 0.128
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski