Thermus phage phiKo

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; unclassified Tectiviridae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8I697|A0A3Q8I697_9VIRU Uncharacterized protein OS=Thermus phage phiKo OX=2301535 GN=phiKo_04 PE=4 SV=1
MM1 pKa = 7.49PEE3 pKa = 3.61EE4 pKa = 4.34MGDD7 pKa = 3.84GFILVYY13 pKa = 10.73GRR15 pKa = 11.84GEE17 pKa = 3.99TEE19 pKa = 3.65EE20 pKa = 4.21ARR22 pKa = 11.84EE23 pKa = 3.89NGYY26 pKa = 10.61LPDD29 pKa = 3.46VDD31 pKa = 4.23EE32 pKa = 4.52YY33 pKa = 11.23RR34 pKa = 11.84ATFATFHH41 pKa = 6.05EE42 pKa = 4.79AYY44 pKa = 9.03MYY46 pKa = 11.13AEE48 pKa = 5.33TIPVPTTIVYY58 pKa = 10.43DD59 pKa = 3.87EE60 pKa = 4.68EE61 pKa = 4.34TDD63 pKa = 3.16TWEE66 pKa = 4.15VYY68 pKa = 10.44VDD70 pKa = 3.74YY71 pKa = 10.86EE72 pKa = 4.09

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8I0V1|A0A3Q8I0V1_9VIRU Uncharacterized protein OS=Thermus phage phiKo OX=2301535 GN=phiKo_09 PE=4 SV=1
MM1 pKa = 7.74TYY3 pKa = 8.55PTIYY7 pKa = 9.71HH8 pKa = 7.06AMRR11 pKa = 11.84HH12 pKa = 5.33YY13 pKa = 10.31YY14 pKa = 10.15LPHH17 pKa = 6.15EE18 pKa = 4.54VAALLRR24 pKa = 11.84GKK26 pKa = 10.69AEE28 pKa = 3.75VRR30 pKa = 11.84EE31 pKa = 4.19EE32 pKa = 3.8EE33 pKa = 4.26GRR35 pKa = 11.84PVVVDD40 pKa = 2.88SEE42 pKa = 4.38GRR44 pKa = 11.84VRR46 pKa = 11.84LKK48 pKa = 10.66AVSVLVEE55 pKa = 3.92IQSRR59 pKa = 11.84WGGATGLATPHH70 pKa = 6.52WAFPMEE76 pKa = 4.55DD77 pKa = 3.27VEE79 pKa = 4.54ALLRR83 pKa = 11.84ALRR86 pKa = 11.84RR87 pKa = 11.84PAKK90 pKa = 9.56GRR92 pKa = 11.84KK93 pKa = 9.11RR94 pKa = 11.84GG95 pKa = 3.42

Molecular weight: 10.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

3378

46

341

129.9

14.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.716 ± 0.739

0.355 ± 0.108

4.026 ± 0.294

7.223 ± 1.09

2.043 ± 0.247

9.177 ± 0.479

1.599 ± 0.327

4.026 ± 0.333

2.931 ± 0.408

10.746 ± 0.557

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.358

2.427 ± 0.404

6.631 ± 0.502

3.02 ± 0.357

7.401 ± 0.645

4.085 ± 0.608

5.21 ± 0.797

8.822 ± 0.535

3.227 ± 0.389

4.204 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski