Beihai picobirna-like virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLD7|A0A1L3KLD7_9VIRU Uncharacterized protein OS=Beihai picobirna-like virus 7 OX=1922524 PE=4 SV=1
MM1 pKa = 7.54NFQFLDD7 pKa = 3.6TLSVSKK13 pKa = 10.57QVEE16 pKa = 4.11NSVSSNLRR24 pKa = 11.84RR25 pKa = 11.84VVSGSEE31 pKa = 4.12DD32 pKa = 3.6VLTSPIVKK40 pKa = 9.98RR41 pKa = 11.84KK42 pKa = 9.96APEE45 pKa = 5.16DD46 pKa = 3.39ILKK49 pKa = 10.54GWDD52 pKa = 3.53EE53 pKa = 3.93IVKK56 pKa = 10.44EE57 pKa = 4.26NLSAMNADD65 pKa = 4.97LINLEE70 pKa = 4.49EE71 pKa = 4.34SNKK74 pKa = 10.52LKK76 pKa = 10.68FGPRR80 pKa = 11.84SIAAPWGEE88 pKa = 3.97RR89 pKa = 11.84TDD91 pKa = 4.21GVLSYY96 pKa = 10.31FAEE99 pKa = 4.58DD100 pKa = 3.68TLFTPPTVSKK110 pKa = 10.68VPRR113 pKa = 11.84RR114 pKa = 11.84NLRR117 pKa = 11.84PISEE121 pKa = 4.05AKK123 pKa = 10.7ALGFLKK129 pKa = 10.79NSTNSGLPYY138 pKa = 8.56YY139 pKa = 8.87TKK141 pKa = 10.25KK142 pKa = 11.04GKK144 pKa = 10.37VKK146 pKa = 10.28EE147 pKa = 4.5IYY149 pKa = 9.31STMQSPLLSRR159 pKa = 11.84KK160 pKa = 9.55DD161 pKa = 3.36PCVMFTRR168 pKa = 11.84TQEE171 pKa = 3.75QRR173 pKa = 11.84KK174 pKa = 6.66TRR176 pKa = 11.84TVWGYY181 pKa = 10.32PMADD185 pKa = 3.69TLRR188 pKa = 11.84EE189 pKa = 3.85MQYY192 pKa = 10.54YY193 pKa = 9.88EE194 pKa = 5.39PILGYY199 pKa = 7.76QRR201 pKa = 11.84KK202 pKa = 9.37LNWRR206 pKa = 11.84NSLSGPDD213 pKa = 3.24AVNRR217 pKa = 11.84KK218 pKa = 8.62MSDD221 pKa = 3.24LVTKK225 pKa = 10.56AISSDD230 pKa = 3.46QYY232 pKa = 11.41LLSIDD237 pKa = 4.27FSAYY241 pKa = 9.55DD242 pKa = 3.59ASVKK246 pKa = 8.23TTLQKK251 pKa = 11.27ASFDD255 pKa = 4.02YY256 pKa = 10.65FKK258 pKa = 11.16SLYY261 pKa = 8.7QADD264 pKa = 3.97YY265 pKa = 10.94HH266 pKa = 7.17EE267 pKa = 5.85EE268 pKa = 3.74IDD270 pKa = 5.72DD271 pKa = 3.44IATRR275 pKa = 11.84FNTIGLVTPDD285 pKa = 4.54GIIDD289 pKa = 4.15GPHH292 pKa = 6.13GVPSGSTFTNEE303 pKa = 3.6VDD305 pKa = 4.53SVAQYY310 pKa = 10.99LIATEE315 pKa = 4.44YY316 pKa = 11.15GLSSDD321 pKa = 4.16NFDD324 pKa = 3.36IQGDD328 pKa = 3.66DD329 pKa = 3.24GAYY332 pKa = 10.01VVSDD336 pKa = 3.85PDD338 pKa = 3.57SLKK341 pKa = 11.1DD342 pKa = 3.46YY343 pKa = 9.98FRR345 pKa = 11.84KK346 pKa = 10.4YY347 pKa = 10.9NLVVNDD353 pKa = 4.01DD354 pKa = 3.4KK355 pKa = 11.94SYY357 pKa = 10.79ISKK360 pKa = 10.76DD361 pKa = 3.34YY362 pKa = 11.17LIYY365 pKa = 10.58LQNLYY370 pKa = 10.44HH371 pKa = 7.03KK372 pKa = 10.48DD373 pKa = 3.53YY374 pKa = 10.38MRR376 pKa = 11.84DD377 pKa = 3.66GIIGGIYY384 pKa = 6.86PTYY387 pKa = 9.93RR388 pKa = 11.84ALNRR392 pKa = 11.84IVYY395 pKa = 9.64LEE397 pKa = 4.28RR398 pKa = 11.84FTDD401 pKa = 4.15FLEE404 pKa = 5.65DD405 pKa = 3.77DD406 pKa = 5.03LKK408 pKa = 11.17GQDD411 pKa = 3.69YY412 pKa = 10.71FSLRR416 pKa = 11.84TISILEE422 pKa = 3.85NCKK425 pKa = 9.72FHH427 pKa = 7.3PLFEE431 pKa = 4.33EE432 pKa = 4.05LVKK435 pKa = 10.97YY436 pKa = 9.72VASLDD441 pKa = 3.76KK442 pKa = 11.49YY443 pKa = 9.59KK444 pKa = 10.98LKK446 pKa = 10.88YY447 pKa = 10.24SNSGLAKK454 pKa = 7.81YY455 pKa = 6.93TQRR458 pKa = 11.84ASQSSGVAGIFKK470 pKa = 10.25YY471 pKa = 10.3RR472 pKa = 11.84YY473 pKa = 8.78EE474 pKa = 5.18DD475 pKa = 4.19DD476 pKa = 4.15PKK478 pKa = 11.42GLNNFDD484 pKa = 3.69TVKK487 pKa = 10.64LLKK490 pKa = 10.39EE491 pKa = 4.01LL492 pKa = 3.98

Molecular weight: 56.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLD7|A0A1L3KLD7_9VIRU Uncharacterized protein OS=Beihai picobirna-like virus 7 OX=1922524 PE=4 SV=1
MM1 pKa = 7.71IYY3 pKa = 10.27SKK5 pKa = 10.81SINKK9 pKa = 9.13VSLTKK14 pKa = 10.61LAAKK18 pKa = 10.16RR19 pKa = 11.84FQDD22 pKa = 3.62DD23 pKa = 4.49PYY25 pKa = 10.99VGMVLRR31 pKa = 11.84QSPEE35 pKa = 3.55FDD37 pKa = 3.26SFDD40 pKa = 3.47VNLRR44 pKa = 11.84SNPSNSDD51 pKa = 3.0SPRR54 pKa = 11.84NMSRR58 pKa = 11.84RR59 pKa = 11.84KK60 pKa = 8.08TSSNKK65 pKa = 9.37KK66 pKa = 8.36PKK68 pKa = 9.71KK69 pKa = 9.72KK70 pKa = 10.58KK71 pKa = 8.03PVQYY75 pKa = 10.27NGPKK79 pKa = 9.26AQRR82 pKa = 11.84ARR84 pKa = 11.84AQAQASSLAQGPFNLPPAAFVPGAPGFRR112 pKa = 11.84PATQPLLPVPTKK124 pKa = 10.85GVGALDD130 pKa = 4.33FAQSVVDD137 pKa = 4.66GIGRR141 pKa = 11.84GAAIVKK147 pKa = 9.12TGADD151 pKa = 3.85AIKK154 pKa = 10.67AIDD157 pKa = 4.07DD158 pKa = 3.62ATGGKK163 pKa = 9.13LRR165 pKa = 11.84PRR167 pKa = 11.84RR168 pKa = 11.84PRR170 pKa = 11.84NPDD173 pKa = 2.86RR174 pKa = 11.84VGGSGGEE181 pKa = 4.05SDD183 pKa = 3.89GSGGGGGGSKK193 pKa = 8.65STNLNNPNGGYY204 pKa = 9.73SYY206 pKa = 11.33TMMCNKK212 pKa = 9.57PVSQKK217 pKa = 10.18IEE219 pKa = 3.49ISTGVRR225 pKa = 11.84SGLIVNPGEE234 pKa = 4.25PNNDD238 pKa = 2.97SDD240 pKa = 4.81FSSLYY245 pKa = 10.35IMSGNLLKK253 pKa = 10.33KK254 pKa = 10.71DD255 pKa = 3.63SVDD258 pKa = 3.05TSFRR262 pKa = 11.84RR263 pKa = 11.84HH264 pKa = 6.0IEE266 pKa = 3.86TTIWPMVNQAVQYY279 pKa = 9.91NLNFEE284 pKa = 4.89YY285 pKa = 10.38ILSLTDD291 pKa = 3.48FNNWFYY297 pKa = 11.83DD298 pKa = 3.26MTKK301 pKa = 10.68ALEE304 pKa = 4.04VYY306 pKa = 9.78YY307 pKa = 10.96ALDD310 pKa = 3.32SVITYY315 pKa = 9.59GEE317 pKa = 4.04KK318 pKa = 10.58VEE320 pKa = 4.16NNNPGSRR327 pKa = 11.84YY328 pKa = 9.52LRR330 pKa = 11.84EE331 pKa = 4.74KK332 pKa = 8.68ITGPIRR338 pKa = 11.84LKK340 pKa = 10.9FNEE343 pKa = 4.5LRR345 pKa = 11.84NILNNQAIPPRR356 pKa = 11.84LIQLVRR362 pKa = 11.84YY363 pKa = 7.79MYY365 pKa = 10.18QYY367 pKa = 11.43YY368 pKa = 9.95KK369 pKa = 10.69FSEE372 pKa = 4.52LPGSPLYY379 pKa = 10.37RR380 pKa = 11.84LSPGFIFCNDD390 pKa = 3.1ANVGDD395 pKa = 4.56DD396 pKa = 4.36AYY398 pKa = 10.31IHH400 pKa = 6.53ALNTAMLDD408 pKa = 3.59KK409 pKa = 11.07LITNLTSHH417 pKa = 6.78SDD419 pKa = 3.47VHH421 pKa = 6.76NKK423 pKa = 9.33IYY425 pKa = 10.76KK426 pKa = 10.28AMPEE430 pKa = 3.64WHH432 pKa = 6.77VSGKK436 pKa = 9.77CLPPSCDD443 pKa = 2.77TAYY446 pKa = 10.76YY447 pKa = 10.28DD448 pKa = 3.77PSFRR452 pKa = 11.84TFWFNSCTSYY462 pKa = 11.03QVGNVVKK469 pKa = 8.31YY470 pKa = 7.94TRR472 pKa = 11.84KK473 pKa = 10.35GDD475 pKa = 3.24GSSNFRR481 pKa = 11.84YY482 pKa = 9.01WLYY485 pKa = 11.0TNNFDD490 pKa = 5.21GLFFALGSFNKK501 pKa = 8.12TTEE504 pKa = 3.76EE505 pKa = 4.26GTRR508 pKa = 11.84PGLWTPPSDD517 pKa = 3.41FSGIVGEE524 pKa = 4.38TNKK527 pKa = 10.22TSVQCFHH534 pKa = 7.31ADD536 pKa = 3.06KK537 pKa = 10.14EE538 pKa = 4.13IRR540 pKa = 11.84RR541 pKa = 11.84IVQLDD546 pKa = 3.46GTYY549 pKa = 10.97GKK551 pKa = 10.45ALQSDD556 pKa = 4.91NILVPYY562 pKa = 10.08QNLGGSGITIQLCHH576 pKa = 6.26NQDD579 pKa = 2.88GSTQVAQEE587 pKa = 4.43CTFNTYY593 pKa = 9.26KK594 pKa = 10.1QAVYY598 pKa = 10.24EE599 pKa = 4.12VTNWIFSII607 pKa = 4.36

Molecular weight: 67.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1099

492

607

549.5

61.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.369 ± 0.322

0.91 ± 0.33

6.733 ± 1.05

4.004 ± 0.707

4.459 ± 0.125

7.188 ± 1.116

1.001 ± 0.123

5.187 ± 0.064

6.824 ± 0.59

8.371 ± 1.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.729 ± 0.067

6.46 ± 1.038

5.46 ± 0.781

3.731 ± 0.314

5.096 ± 0.009

9.008 ± 0.224

5.823 ± 0.087

5.914 ± 0.12

1.001 ± 0.123

5.732 ± 0.64
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski