Amino acid dipepetide frequency for Flavobacterium humi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.101AlaAla: 5.101 ± 0.098
0.618AlaCys: 0.618 ± 0.03
3.434AlaAsp: 3.434 ± 0.077
4.039AlaGlu: 4.039 ± 0.093
3.643AlaPhe: 3.643 ± 0.051
4.847AlaGly: 4.847 ± 0.103
1.105AlaHis: 1.105 ± 0.039
5.36AlaIle: 5.36 ± 0.084
5.073AlaLys: 5.073 ± 0.113
6.474AlaLeu: 6.474 ± 0.082
1.547AlaMet: 1.547 ± 0.043
3.924AlaAsn: 3.924 ± 0.098
2.212AlaPro: 2.212 ± 0.065
2.714AlaGln: 2.714 ± 0.05
2.034AlaArg: 2.034 ± 0.05
4.631AlaSer: 4.631 ± 0.082
4.439AlaThr: 4.439 ± 0.122
4.647AlaVal: 4.647 ± 0.067
0.617AlaTrp: 0.617 ± 0.025
2.412AlaTyr: 2.412 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.552CysAla: 0.552 ± 0.036
0.112CysCys: 0.112 ± 0.011
0.494CysAsp: 0.494 ± 0.042
0.455CysGlu: 0.455 ± 0.023
0.456CysPhe: 0.456 ± 0.026
0.701CysGly: 0.701 ± 0.033
0.215CysHis: 0.215 ± 0.016
0.641CysIle: 0.641 ± 0.025
0.491CysLys: 0.491 ± 0.02
0.729CysLeu: 0.729 ± 0.032
0.131CysMet: 0.131 ± 0.011
0.485CysAsn: 0.485 ± 0.029
0.391CysPro: 0.391 ± 0.026
0.256CysGln: 0.256 ± 0.015
0.229CysArg: 0.229 ± 0.013
0.626CysSer: 0.626 ± 0.032
0.581CysThr: 0.581 ± 0.037
0.459CysVal: 0.459 ± 0.029
0.08CysTrp: 0.08 ± 0.009
0.344CysTyr: 0.344 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.865AspAla: 3.865 ± 0.08
0.466AspCys: 0.466 ± 0.029
2.579AspAsp: 2.579 ± 0.061
3.15AspGlu: 3.15 ± 0.06
3.722AspPhe: 3.722 ± 0.065
3.59AspGly: 3.59 ± 0.09
0.822AspHis: 0.822 ± 0.031
4.088AspIle: 4.088 ± 0.066
4.065AspLys: 4.065 ± 0.076
4.828AspLeu: 4.828 ± 0.076
1.059AspMet: 1.059 ± 0.035
3.021AspAsn: 3.021 ± 0.065
1.671AspPro: 1.671 ± 0.049
1.485AspGln: 1.485 ± 0.037
1.722AspArg: 1.722 ± 0.045
3.003AspSer: 3.003 ± 0.064
2.755AspThr: 2.755 ± 0.055
3.339AspVal: 3.339 ± 0.062
0.663AspTrp: 0.663 ± 0.025
2.752AspTyr: 2.752 ± 0.055
0.0AspXaa: 0.0 ± 0.0
Glu
4.361GluAla: 4.361 ± 0.085
0.334GluCys: 0.334 ± 0.017
3.074GluAsp: 3.074 ± 0.063
4.699GluGlu: 4.699 ± 0.088
2.811GluPhe: 2.811 ± 0.054
3.587GluGly: 3.587 ± 0.068
1.109GluHis: 1.109 ± 0.037
5.472GluIle: 5.472 ± 0.106
6.216GluLys: 6.216 ± 0.096
5.465GluLeu: 5.465 ± 0.088
1.656GluMet: 1.656 ± 0.043
4.742GluAsn: 4.742 ± 0.074
1.484GluPro: 1.484 ± 0.037
2.222GluGln: 2.222 ± 0.054
2.236GluArg: 2.236 ± 0.057
3.09GluSer: 3.09 ± 0.056
3.726GluThr: 3.726 ± 0.072
3.93GluVal: 3.93 ± 0.073
0.58GluTrp: 0.58 ± 0.021
2.349GluTyr: 2.349 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.358PheAla: 3.358 ± 0.057
0.52PheCys: 0.52 ± 0.023
3.239PheAsp: 3.239 ± 0.062
3.588PheGlu: 3.588 ± 0.077
2.796PhePhe: 2.796 ± 0.07
3.619PheGly: 3.619 ± 0.07
0.88PheHis: 0.88 ± 0.028
3.613PheIle: 3.613 ± 0.066
3.34PheLys: 3.34 ± 0.06
4.807PheLeu: 4.807 ± 0.083
1.115PheMet: 1.115 ± 0.033
3.167PheAsn: 3.167 ± 0.06
1.928PhePro: 1.928 ± 0.048
1.707PheGln: 1.707 ± 0.04
1.768PheArg: 1.768 ± 0.044
4.274PheSer: 4.274 ± 0.079
3.382PheThr: 3.382 ± 0.076
3.027PheVal: 3.027 ± 0.056
0.537PheTrp: 0.537 ± 0.025
2.173PheTyr: 2.173 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
4.252GlyAla: 4.252 ± 0.095
0.768GlyCys: 0.768 ± 0.05
3.003GlyAsp: 3.003 ± 0.06
3.306GlyGlu: 3.306 ± 0.065
3.743GlyPhe: 3.743 ± 0.07
4.505GlyGly: 4.505 ± 0.112
1.18GlyHis: 1.18 ± 0.038
5.59GlyIle: 5.59 ± 0.098
5.384GlyLys: 5.384 ± 0.095
5.695GlyLeu: 5.695 ± 0.096
1.664GlyMet: 1.664 ± 0.039
4.121GlyAsn: 4.121 ± 0.084
1.547GlyPro: 1.547 ± 0.106
2.108GlyGln: 2.108 ± 0.052
1.931GlyArg: 1.931 ± 0.048
4.19GlySer: 4.19 ± 0.087
4.613GlyThr: 4.613 ± 0.129
3.94GlyVal: 3.94 ± 0.075
0.701GlyTrp: 0.701 ± 0.028
2.791GlyTyr: 2.791 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
1.04HisAla: 1.04 ± 0.034
0.196HisCys: 0.196 ± 0.014
0.92HisAsp: 0.92 ± 0.031
0.969HisGlu: 0.969 ± 0.03
1.261HisPhe: 1.261 ± 0.038
1.026HisGly: 1.026 ± 0.034
0.502HisHis: 0.502 ± 0.023
1.377HisIle: 1.377 ± 0.04
1.174HisLys: 1.174 ± 0.038
1.757HisLeu: 1.757 ± 0.045
0.336HisMet: 0.336 ± 0.016
0.955HisAsn: 0.955 ± 0.028
0.859HisPro: 0.859 ± 0.027
0.735HisGln: 0.735 ± 0.028
0.642HisArg: 0.642 ± 0.024
1.156HisSer: 1.156 ± 0.033
1.003HisThr: 1.003 ± 0.031
0.848HisVal: 0.848 ± 0.03
0.248HisTrp: 0.248 ± 0.014
0.896HisTyr: 0.896 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.76IleAla: 5.76 ± 0.077
0.663IleCys: 0.663 ± 0.026
4.504IleAsp: 4.504 ± 0.082
5.075IleGlu: 5.075 ± 0.079
3.313IlePhe: 3.313 ± 0.061
4.993IleGly: 4.993 ± 0.082
1.372IleHis: 1.372 ± 0.037
5.692IleIle: 5.692 ± 0.099
5.369IleLys: 5.369 ± 0.081
6.693IleLeu: 6.693 ± 0.106
1.36IleMet: 1.36 ± 0.04
4.42IleAsn: 4.42 ± 0.08
3.269IlePro: 3.269 ± 0.059
2.738IleGln: 2.738 ± 0.056
2.803IleArg: 2.803 ± 0.06
5.444IleSer: 5.444 ± 0.084
4.951IleThr: 4.951 ± 0.091
4.924IleVal: 4.924 ± 0.075
0.642IleTrp: 0.642 ± 0.025
2.593IleTyr: 2.593 ± 0.052
0.0IleXaa: 0.0 ± 0.0
Lys
5.247LysAla: 5.247 ± 0.113
0.394LysCys: 0.394 ± 0.019
4.098LysAsp: 4.098 ± 0.075
6.086LysGlu: 6.086 ± 0.107
2.909LysPhe: 2.909 ± 0.056
4.484LysGly: 4.484 ± 0.078
1.226LysHis: 1.226 ± 0.034
6.66LysIle: 6.66 ± 0.106
6.886LysLys: 6.886 ± 0.125
6.093LysLeu: 6.093 ± 0.083
2.161LysMet: 2.161 ± 0.055
5.189LysAsn: 5.189 ± 0.084
2.638LysPro: 2.638 ± 0.056
2.619LysGln: 2.619 ± 0.056
2.48LysArg: 2.48 ± 0.057
4.29LysSer: 4.29 ± 0.068
4.998LysThr: 4.998 ± 0.081
4.409LysVal: 4.409 ± 0.06
0.713LysTrp: 0.713 ± 0.027
2.982LysTyr: 2.982 ± 0.065
0.0LysXaa: 0.0 ± 0.0
Leu
6.184LeuAla: 6.184 ± 0.081
0.747LeuCys: 0.747 ± 0.027
4.878LeuAsp: 4.878 ± 0.078
5.797LeuGlu: 5.797 ± 0.088
4.876LeuPhe: 4.876 ± 0.084
5.624LeuGly: 5.624 ± 0.087
1.697LeuHis: 1.697 ± 0.046
6.07LeuIle: 6.07 ± 0.105
7.084LeuLys: 7.084 ± 0.104
8.771LeuLeu: 8.771 ± 0.132
2.029LeuMet: 2.029 ± 0.051
5.13LeuAsn: 5.13 ± 0.085
3.666LeuPro: 3.666 ± 0.063
3.525LeuGln: 3.525 ± 0.061
2.88LeuArg: 2.88 ± 0.056
6.618LeuSer: 6.618 ± 0.084
5.127LeuThr: 5.127 ± 0.083
5.229LeuVal: 5.229 ± 0.086
0.792LeuTrp: 0.792 ± 0.032
3.283LeuTyr: 3.283 ± 0.063
0.0LeuXaa: 0.0 ± 0.0
Met
1.714MetAla: 1.714 ± 0.043
0.137MetCys: 0.137 ± 0.012
1.117MetAsp: 1.117 ± 0.035
1.554MetGlu: 1.554 ± 0.044
0.812MetPhe: 0.812 ± 0.027
1.369MetGly: 1.369 ± 0.046
0.446MetHis: 0.446 ± 0.017
1.51MetIle: 1.51 ± 0.041
2.194MetLys: 2.194 ± 0.05
1.825MetLeu: 1.825 ± 0.045
0.622MetMet: 0.622 ± 0.028
1.28MetAsn: 1.28 ± 0.034
0.871MetPro: 0.871 ± 0.033
0.885MetGln: 0.885 ± 0.03
0.795MetArg: 0.795 ± 0.031
1.391MetSer: 1.391 ± 0.038
1.261MetThr: 1.261 ± 0.043
1.428MetVal: 1.428 ± 0.043
0.148MetTrp: 0.148 ± 0.012
0.75MetTyr: 0.75 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
4.18AsnAla: 4.18 ± 0.083
0.56AsnCys: 0.56 ± 0.031
3.124AsnAsp: 3.124 ± 0.062
3.494AsnGlu: 3.494 ± 0.061
2.932AsnPhe: 2.932 ± 0.059
4.524AsnGly: 4.524 ± 0.131
1.148AsnHis: 1.148 ± 0.034
4.405AsnIle: 4.405 ± 0.066
4.05AsnLys: 4.05 ± 0.071
5.25AsnLeu: 5.25 ± 0.088
1.177AsnMet: 1.177 ± 0.034
3.859AsnAsn: 3.859 ± 0.089
3.148AsnPro: 3.148 ± 0.072
2.351AsnGln: 2.351 ± 0.051
2.093AsnArg: 2.093 ± 0.047
3.782AsnSer: 3.782 ± 0.073
4.02AsnThr: 4.02 ± 0.079
3.644AsnVal: 3.644 ± 0.07
0.769AsnTrp: 0.769 ± 0.033
2.901AsnTyr: 2.901 ± 0.066
0.0AsnXaa: 0.0 ± 0.0
Pro
2.603ProAla: 2.603 ± 0.075
0.237ProCys: 0.237 ± 0.018
2.093ProAsp: 2.093 ± 0.049
2.853ProGlu: 2.853 ± 0.057
2.072ProPhe: 2.072 ± 0.049
2.186ProGly: 2.186 ± 0.055
0.56ProHis: 0.56 ± 0.022
2.594ProIle: 2.594 ± 0.049
2.602ProLys: 2.602 ± 0.058
2.891ProLeu: 2.891 ± 0.055
0.797ProMet: 0.797 ± 0.043
2.323ProAsn: 2.323 ± 0.062
0.82ProPro: 0.82 ± 0.036
1.29ProGln: 1.29 ± 0.049
0.854ProArg: 0.854 ± 0.031
2.332ProSer: 2.332 ± 0.058
2.186ProThr: 2.186 ± 0.096
2.879ProVal: 2.879 ± 0.064
0.302ProTrp: 0.302 ± 0.019
1.422ProTyr: 1.422 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.208GlnAla: 2.208 ± 0.049
0.253GlnCys: 0.253 ± 0.028
1.845GlnAsp: 1.845 ± 0.048
2.401GlnGlu: 2.401 ± 0.055
1.819GlnPhe: 1.819 ± 0.042
1.93GlnGly: 1.93 ± 0.068
0.649GlnHis: 0.649 ± 0.028
2.545GlnIle: 2.545 ± 0.049
3.222GlnLys: 3.222 ± 0.067
3.569GlnLeu: 3.569 ± 0.07
0.902GlnMet: 0.902 ± 0.03
2.419GlnAsn: 2.419 ± 0.057
1.166GlnPro: 1.166 ± 0.038
1.518GlnGln: 1.518 ± 0.046
1.109GlnArg: 1.109 ± 0.032
2.125GlnSer: 2.125 ± 0.047
2.059GlnThr: 2.059 ± 0.045
2.125GlnVal: 2.125 ± 0.052
0.443GlnTrp: 0.443 ± 0.024
1.549GlnTyr: 1.549 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
1.888ArgAla: 1.888 ± 0.05
0.179ArgCys: 0.179 ± 0.014
1.653ArgAsp: 1.653 ± 0.042
2.072ArgGlu: 2.072 ± 0.05
1.873ArgPhe: 1.873 ± 0.048
1.698ArgGly: 1.698 ± 0.048
0.625ArgHis: 0.625 ± 0.025
2.81ArgIle: 2.81 ± 0.058
2.88ArgLys: 2.88 ± 0.059
3.102ArgLeu: 3.102 ± 0.06
0.967ArgMet: 0.967 ± 0.027
2.149ArgAsn: 2.149 ± 0.053
1.019ArgPro: 1.019 ± 0.036
1.136ArgGln: 1.136 ± 0.034
1.146ArgArg: 1.146 ± 0.038
1.699ArgSer: 1.699 ± 0.042
1.695ArgThr: 1.695 ± 0.043
1.88ArgVal: 1.88 ± 0.047
0.348ArgTrp: 0.348 ± 0.017
1.447ArgTyr: 1.447 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
4.198SerAla: 4.198 ± 0.077
0.74SerCys: 0.74 ± 0.035
3.37SerAsp: 3.37 ± 0.056
3.739SerGlu: 3.739 ± 0.057
3.916SerPhe: 3.916 ± 0.071
5.038SerGly: 5.038 ± 0.104
1.296SerHis: 1.296 ± 0.04
4.823SerIle: 4.823 ± 0.069
4.601SerLys: 4.601 ± 0.073
5.885SerLeu: 5.885 ± 0.085
1.307SerMet: 1.307 ± 0.037
3.862SerAsn: 3.862 ± 0.093
2.258SerPro: 2.258 ± 0.047
2.418SerGln: 2.418 ± 0.056
2.085SerArg: 2.085 ± 0.056
4.158SerSer: 4.158 ± 0.092
3.506SerThr: 3.506 ± 0.082
4.356SerVal: 4.356 ± 0.086
0.715SerTrp: 0.715 ± 0.024
2.702SerTyr: 2.702 ± 0.067
0.0SerXaa: 0.0 ± 0.0
Thr
4.599ThrAla: 4.599 ± 0.117
0.453ThrCys: 0.453 ± 0.036
3.314ThrAsp: 3.314 ± 0.067
3.574ThrGlu: 3.574 ± 0.056
3.198ThrPhe: 3.198 ± 0.06
4.63ThrGly: 4.63 ± 0.138
1.023ThrHis: 1.023 ± 0.03
5.252ThrIle: 5.252 ± 0.108
3.774ThrLys: 3.774 ± 0.066
5.407ThrLeu: 5.407 ± 0.087
1.017ThrMet: 1.017 ± 0.033
3.473ThrAsn: 3.473 ± 0.084
2.802ThrPro: 2.802 ± 0.09
2.094ThrGln: 2.094 ± 0.064
1.617ThrArg: 1.617 ± 0.046
4.034ThrSer: 4.034 ± 0.091
4.121ThrThr: 4.121 ± 0.123
4.526ThrVal: 4.526 ± 0.114
0.574ThrTrp: 0.574 ± 0.026
2.51ThrTyr: 2.51 ± 0.073
0.0ThrXaa: 0.0 ± 0.0
Val
4.558ValAla: 4.558 ± 0.079
0.567ValCys: 0.567 ± 0.024
3.016ValAsp: 3.016 ± 0.066
3.471ValGlu: 3.471 ± 0.062
3.619ValPhe: 3.619 ± 0.06
3.543ValGly: 3.543 ± 0.065
0.982ValHis: 0.982 ± 0.031
4.613ValIle: 4.613 ± 0.073
4.441ValLys: 4.441 ± 0.063
6.11ValLeu: 6.11 ± 0.087
1.268ValMet: 1.268 ± 0.037
3.617ValAsn: 3.617 ± 0.071
2.405ValPro: 2.405 ± 0.05
1.977ValGln: 1.977 ± 0.055
2.005ValArg: 2.005 ± 0.05
4.79ValSer: 4.79 ± 0.087
4.28ValThr: 4.28 ± 0.128
4.167ValVal: 4.167 ± 0.082
0.598ValTrp: 0.598 ± 0.026
2.567ValTyr: 2.567 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.658TrpAla: 0.658 ± 0.026
0.103TrpCys: 0.103 ± 0.01
0.557TrpAsp: 0.557 ± 0.024
0.612TrpGlu: 0.612 ± 0.024
0.56TrpPhe: 0.56 ± 0.025
0.628TrpGly: 0.628 ± 0.03
0.234TrpHis: 0.234 ± 0.014
0.686TrpIle: 0.686 ± 0.029
0.855TrpLys: 0.855 ± 0.029
0.906TrpLeu: 0.906 ± 0.032
0.267TrpMet: 0.267 ± 0.014
0.706TrpAsn: 0.706 ± 0.029
0.202TrpPro: 0.202 ± 0.017
0.425TrpGln: 0.425 ± 0.021
0.337TrpArg: 0.337 ± 0.018
0.626TrpSer: 0.626 ± 0.028
0.592TrpThr: 0.592 ± 0.03
0.581TrpVal: 0.581 ± 0.025
0.127TrpTrp: 0.127 ± 0.011
0.413TrpTyr: 0.413 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.546TyrAla: 2.546 ± 0.052
0.383TyrCys: 0.383 ± 0.021
2.333TyrAsp: 2.333 ± 0.058
2.209TyrGlu: 2.209 ± 0.049
2.505TyrPhe: 2.505 ± 0.057
2.506TyrGly: 2.506 ± 0.057
0.828TyrHis: 0.828 ± 0.03
2.778TyrIle: 2.778 ± 0.053
2.863TyrLys: 2.863 ± 0.062
3.749TyrLeu: 3.749 ± 0.06
0.726TyrMet: 0.726 ± 0.028
2.568TyrAsn: 2.568 ± 0.067
1.538TyrPro: 1.538 ± 0.037
1.654TyrGln: 1.654 ± 0.044
1.503TyrArg: 1.503 ± 0.044
2.715TyrSer: 2.715 ± 0.063
2.698TyrThr: 2.698 ± 0.084
2.258TyrVal: 2.258 ± 0.051
0.479TyrTrp: 0.479 ± 0.021
1.992TyrTyr: 1.992 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3054 proteins (1069261 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski