Candidatus Izimaplasma sp. HR1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Tenericutes incertae sedis; Candidatus Izimaplasma; unclassified Candidatus Izimaplasma

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1811 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088T012|A0A088T012_9BACT Recombination protein RecR OS=Candidatus Izimaplasma sp. HR1 OX=1541959 GN=recR PE=3 SV=1
MM1 pKa = 7.39KK2 pKa = 10.37KK3 pKa = 10.3VLTLLLVFTLGLTLASCTDD22 pKa = 3.29QEE24 pKa = 4.46AATNVADD31 pKa = 4.22AAQKK35 pKa = 10.86LSLEE39 pKa = 4.37VSDD42 pKa = 4.22PTAVTSDD49 pKa = 2.62ITLPTEE55 pKa = 4.35GLHH58 pKa = 6.47EE59 pKa = 4.38STISWEE65 pKa = 4.14SDD67 pKa = 2.69NAAISAAGVVTRR79 pKa = 11.84PAIGSDD85 pKa = 3.36DD86 pKa = 3.56VTVILTATFTIGKK99 pKa = 7.05EE100 pKa = 4.11TTTKK104 pKa = 10.56EE105 pKa = 3.83FTFKK109 pKa = 10.73VLAAVPSVAVTAAVFEE125 pKa = 4.61SSATAVGDD133 pKa = 3.88VVEE136 pKa = 4.26LTGIVVGLVKK146 pKa = 10.11TYY148 pKa = 10.44GYY150 pKa = 10.3EE151 pKa = 3.91IYY153 pKa = 10.47DD154 pKa = 3.66GTGFTYY160 pKa = 10.75VNEE163 pKa = 4.38FQDD166 pKa = 3.9PTVSIGDD173 pKa = 3.83EE174 pKa = 4.14VTVMGEE180 pKa = 3.53KK181 pKa = 10.38GIYY184 pKa = 9.86NNGSEE189 pKa = 4.2LVEE192 pKa = 4.1IQSTVVNSTGNALPNFTEE210 pKa = 4.26TTIGEE215 pKa = 4.37IFAQDD220 pKa = 3.88PDD222 pKa = 3.94TTDD225 pKa = 2.65WYY227 pKa = 11.02HH228 pKa = 8.59DD229 pKa = 3.54MFNAEE234 pKa = 4.06GVIVVQGDD242 pKa = 3.43YY243 pKa = 11.62DD244 pKa = 3.58NAYY247 pKa = 10.65LSWFDD252 pKa = 3.03QDD254 pKa = 4.0LNLRR258 pKa = 11.84QMEE261 pKa = 4.74IYY263 pKa = 9.97YY264 pKa = 10.48KK265 pKa = 10.68SYY267 pKa = 11.63DD268 pKa = 3.78DD269 pKa = 4.58AKK271 pKa = 10.67LAAVKK276 pKa = 10.34ALEE279 pKa = 4.3GKK281 pKa = 10.03KK282 pKa = 10.11VEE284 pKa = 4.8LDD286 pKa = 4.6FILKK290 pKa = 10.05DD291 pKa = 3.37YY292 pKa = 10.92SYY294 pKa = 11.79GSFRR298 pKa = 11.84LTVNSTGEE306 pKa = 4.07VNEE309 pKa = 3.82ATIYY313 pKa = 10.53TDD315 pKa = 3.68AEE317 pKa = 4.15RR318 pKa = 11.84AKK320 pKa = 10.89LAVAYY325 pKa = 10.54NDD327 pKa = 4.3LFFNSLGDD335 pKa = 3.76EE336 pKa = 4.55VVASLDD342 pKa = 3.98LPTDD346 pKa = 3.35SDD348 pKa = 4.5FVTKK352 pKa = 10.86YY353 pKa = 11.29DD354 pKa = 3.76DD355 pKa = 3.65TEE357 pKa = 4.08EE358 pKa = 5.16ANMPIEE364 pKa = 4.53LVWTSSNEE372 pKa = 3.72VVIANDD378 pKa = 3.72GTVTLPVGATADD390 pKa = 3.57VEE392 pKa = 4.53LTVTATVGDD401 pKa = 3.99VSAHH405 pKa = 4.42KK406 pKa = 9.38TYY408 pKa = 10.39TVTVADD414 pKa = 4.78ADD416 pKa = 4.11TLTTNTVAEE425 pKa = 4.78VIAMEE430 pKa = 4.88DD431 pKa = 3.84GEE433 pKa = 4.83TVIVEE438 pKa = 4.68AIVSGLRR445 pKa = 11.84YY446 pKa = 10.14GNPFVQDD453 pKa = 3.29ADD455 pKa = 3.77GTAMFIYY462 pKa = 9.82TKK464 pKa = 10.74LDD466 pKa = 3.33VKK468 pKa = 11.13VGDD471 pKa = 4.06KK472 pKa = 10.85VWIQGEE478 pKa = 4.17LATFSSYY485 pKa = 11.4GNALKK490 pKa = 10.58QLTRR494 pKa = 11.84GVLVKK499 pKa = 9.83TVSTGNALTYY509 pKa = 9.1NTTTTAAEE517 pKa = 3.66LAAAINTMHH526 pKa = 6.99SDD528 pKa = 3.5SFTMTLKK535 pKa = 10.64FLEE538 pKa = 4.5EE539 pKa = 3.87DD540 pKa = 2.95FGYY543 pKa = 10.95YY544 pKa = 10.03FFEE547 pKa = 6.18AYY549 pKa = 10.49VDD551 pKa = 5.29GDD553 pKa = 3.86DD554 pKa = 4.12ADD556 pKa = 3.87NNVRR560 pKa = 11.84IKK562 pKa = 10.27MDD564 pKa = 3.86TDD566 pKa = 3.71WFPTMEE572 pKa = 4.35TYY574 pKa = 11.21LDD576 pKa = 4.02GSDD579 pKa = 3.62MIEE582 pKa = 3.83ISFVLYY588 pKa = 10.27DD589 pKa = 3.13VHH591 pKa = 7.96YY592 pKa = 10.2DD593 pKa = 3.39EE594 pKa = 6.02ARR596 pKa = 11.84IVPVAFPGLTNADD609 pKa = 3.64RR610 pKa = 11.84LDD612 pKa = 3.49IVKK615 pKa = 10.42GMIALEE621 pKa = 4.01EE622 pKa = 4.52FIVLDD627 pKa = 4.07VNLLTYY633 pKa = 10.86LPTFDD638 pKa = 5.98AAITWVSANTAMDD651 pKa = 3.55AEE653 pKa = 4.9GNVTRR658 pKa = 11.84PVAGEE663 pKa = 4.16DD664 pKa = 3.52NVEE667 pKa = 4.13GDD669 pKa = 3.77LTASIVIGSDD679 pKa = 2.97AAVDD683 pKa = 3.59EE684 pKa = 5.23VYY686 pKa = 10.3TVTVVASPTDD696 pKa = 3.18AMTVAEE702 pKa = 5.1VYY704 pKa = 10.35AAGYY708 pKa = 9.92SDD710 pKa = 4.44YY711 pKa = 11.13FGDD714 pKa = 3.8YY715 pKa = 7.83THH717 pKa = 6.91VYY719 pKa = 7.46VTGVVAGLTDD729 pKa = 4.21DD730 pKa = 5.05GYY732 pKa = 11.62VIQDD736 pKa = 3.57TVSGKK741 pKa = 10.3FLVVEE746 pKa = 4.47DD747 pKa = 4.59FDD749 pKa = 5.12NEE751 pKa = 4.37VEE753 pKa = 4.63VGQEE757 pKa = 3.69LNLEE761 pKa = 4.19GFYY764 pKa = 10.6NASYY768 pKa = 10.66EE769 pKa = 4.36VIKK772 pKa = 11.02LRR774 pKa = 11.84DD775 pKa = 3.29VFVFMFVSEE784 pKa = 4.38GNALNLEE791 pKa = 4.25LTDD794 pKa = 4.01AVAMDD799 pKa = 4.09WATFDD804 pKa = 4.07RR805 pKa = 11.84NDD807 pKa = 3.5YY808 pKa = 8.76QTGEE812 pKa = 4.73LIAITAPWATLYY824 pKa = 11.19SGDD827 pKa = 3.42TSYY830 pKa = 11.64LRR832 pKa = 11.84LGNGDD837 pKa = 3.47GLEE840 pKa = 4.14NAKK843 pKa = 10.72SFDD846 pKa = 3.32GKK848 pKa = 11.08YY849 pKa = 10.56VGLQNGANEE858 pKa = 4.33LNLTGTLADD867 pKa = 4.24IFDD870 pKa = 4.52GGQTSTEE877 pKa = 4.14YY878 pKa = 11.39ADD880 pKa = 3.21VTIYY884 pKa = 11.15VLIYY888 pKa = 9.23DD889 pKa = 4.1TTSSYY894 pKa = 11.85NKK896 pKa = 10.15FIILSDD902 pKa = 3.68DD903 pKa = 4.36FIVVTPP909 pKa = 4.54

Molecular weight: 98.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088SZL1|A0A088SZL1_9BACT Probable membrane transporter protein OS=Candidatus Izimaplasma sp. HR1 OX=1541959 GN=KQ51_00185 PE=3 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.41KK4 pKa = 10.39SMKK7 pKa = 9.28VKK9 pKa = 9.97QRR11 pKa = 11.84RR12 pKa = 11.84GSKK15 pKa = 10.15FSARR19 pKa = 11.84EE20 pKa = 3.52YY21 pKa = 9.96TRR23 pKa = 11.84CEE25 pKa = 3.78RR26 pKa = 11.84CGRR29 pKa = 11.84PHH31 pKa = 6.43AVYY34 pKa = 10.39RR35 pKa = 11.84KK36 pKa = 9.55FKK38 pKa = 10.41LCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.94LAYY49 pKa = 10.67LGQIPGVKK57 pKa = 9.32KK58 pKa = 10.9ASWW61 pKa = 3.03

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1811

0

1811

571813

34

2255

315.7

35.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.256 ± 0.06

0.761 ± 0.021

6.028 ± 0.052

7.213 ± 0.061

4.943 ± 0.054

6.155 ± 0.06

1.699 ± 0.022

9.378 ± 0.058

7.712 ± 0.088

9.614 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.025

5.808 ± 0.055

2.817 ± 0.03

2.486 ± 0.026

3.569 ± 0.042

6.089 ± 0.043

5.658 ± 0.048

6.986 ± 0.045

0.658 ± 0.019

4.654 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski