Amino acid dipepetide frequency for Mycobacterium phage Priscilla

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.119AlaAla: 14.119 ± 1.589
1.181AlaCys: 1.181 ± 0.27
7.301AlaAsp: 7.301 ± 0.651
6.818AlaGlu: 6.818 ± 0.612
2.845AlaPhe: 2.845 ± 0.44
9.771AlaGly: 9.771 ± 1.118
2.577AlaHis: 2.577 ± 0.403
4.51AlaIle: 4.51 ± 0.515
4.456AlaLys: 4.456 ± 0.45
7.731AlaLeu: 7.731 ± 0.7
2.147AlaMet: 2.147 ± 0.343
2.953AlaAsn: 2.953 ± 0.433
4.349AlaPro: 4.349 ± 0.519
3.275AlaGln: 3.275 ± 0.45
7.516AlaArg: 7.516 ± 0.656
5.315AlaSer: 5.315 ± 0.577
6.496AlaThr: 6.496 ± 0.499
6.872AlaVal: 6.872 ± 0.515
2.147AlaTrp: 2.147 ± 0.304
2.255AlaTyr: 2.255 ± 0.286
0.0AlaXaa: 0.0 ± 0.0
Cys
0.805CysAla: 0.805 ± 0.233
0.0CysCys: 0.0 ± 0.0
1.235CysAsp: 1.235 ± 0.337
0.859CysGlu: 0.859 ± 0.228
0.215CysPhe: 0.215 ± 0.106
1.986CysGly: 1.986 ± 0.485
0.322CysHis: 0.322 ± 0.147
0.107CysIle: 0.107 ± 0.076
0.698CysLys: 0.698 ± 0.207
0.591CysLeu: 0.591 ± 0.224
0.107CysMet: 0.107 ± 0.073
0.429CysAsn: 0.429 ± 0.15
1.503CysPro: 1.503 ± 0.278
0.268CysGln: 0.268 ± 0.12
0.537CysArg: 0.537 ± 0.201
0.913CysSer: 0.913 ± 0.262
0.591CysThr: 0.591 ± 0.186
0.805CysVal: 0.805 ± 0.21
0.322CysTrp: 0.322 ± 0.135
0.322CysTyr: 0.322 ± 0.135
0.0CysXaa: 0.0 ± 0.0
Asp
6.389AspAla: 6.389 ± 0.64
0.966AspCys: 0.966 ± 0.246
4.402AspAsp: 4.402 ± 0.547
3.919AspGlu: 3.919 ± 0.505
1.718AspPhe: 1.718 ± 0.295
6.389AspGly: 6.389 ± 0.771
1.235AspHis: 1.235 ± 0.28
2.147AspIle: 2.147 ± 0.348
1.718AspLys: 1.718 ± 0.355
5.53AspLeu: 5.53 ± 0.484
1.074AspMet: 1.074 ± 0.25
1.933AspAsn: 1.933 ± 0.353
5.046AspPro: 5.046 ± 0.584
2.308AspGln: 2.308 ± 0.336
4.885AspArg: 4.885 ± 0.67
3.704AspSer: 3.704 ± 0.507
3.865AspThr: 3.865 ± 0.534
4.51AspVal: 4.51 ± 0.487
1.503AspTrp: 1.503 ± 0.299
1.772AspTyr: 1.772 ± 0.336
0.0AspXaa: 0.0 ± 0.0
Glu
6.335GluAla: 6.335 ± 0.733
1.02GluCys: 1.02 ± 0.359
3.167GluAsp: 3.167 ± 0.395
3.167GluGlu: 3.167 ± 0.504
2.147GluPhe: 2.147 ± 0.315
3.275GluGly: 3.275 ± 0.392
1.879GluHis: 1.879 ± 0.403
2.255GluIle: 2.255 ± 0.386
1.933GluLys: 1.933 ± 0.306
5.637GluLeu: 5.637 ± 0.668
1.664GluMet: 1.664 ± 0.289
1.772GluAsn: 1.772 ± 0.299
3.543GluPro: 3.543 ± 0.489
2.738GluGln: 2.738 ± 0.31
4.778GluArg: 4.778 ± 0.537
2.953GluSer: 2.953 ± 0.439
4.134GluThr: 4.134 ± 0.603
4.08GluVal: 4.08 ± 0.473
1.772GluTrp: 1.772 ± 0.308
1.611GluTyr: 1.611 ± 0.276
0.0GluXaa: 0.0 ± 0.0
Phe
3.06PheAla: 3.06 ± 0.435
0.215PheCys: 0.215 ± 0.101
2.362PheAsp: 2.362 ± 0.399
1.557PheGlu: 1.557 ± 0.305
0.859PhePhe: 0.859 ± 0.215
2.631PheGly: 2.631 ± 0.602
0.376PheHis: 0.376 ± 0.134
1.503PheIle: 1.503 ± 0.336
0.966PheLys: 0.966 ± 0.226
1.933PheLeu: 1.933 ± 0.29
0.698PheMet: 0.698 ± 0.179
1.127PheAsn: 1.127 ± 0.33
1.45PhePro: 1.45 ± 0.299
1.342PheGln: 1.342 ± 0.362
1.879PheArg: 1.879 ± 0.336
1.235PheSer: 1.235 ± 0.255
2.416PheThr: 2.416 ± 0.364
2.147PheVal: 2.147 ± 0.29
0.376PheTrp: 0.376 ± 0.135
0.859PheTyr: 0.859 ± 0.254
0.0PheXaa: 0.0 ± 0.0
Gly
8.16GlyAla: 8.16 ± 1.157
1.181GlyCys: 1.181 ± 0.293
5.422GlyAsp: 5.422 ± 0.594
5.154GlyGlu: 5.154 ± 0.668
2.255GlyPhe: 2.255 ± 0.33
10.415GlyGly: 10.415 ± 2.324
1.825GlyHis: 1.825 ± 0.336
3.704GlyIle: 3.704 ± 0.605
2.416GlyLys: 2.416 ± 0.305
5.798GlyLeu: 5.798 ± 0.543
2.577GlyMet: 2.577 ± 0.507
2.792GlyAsn: 2.792 ± 0.394
4.241GlyPro: 4.241 ± 0.627
2.416GlyGln: 2.416 ± 0.557
5.798GlyArg: 5.798 ± 0.564
6.174GlySer: 6.174 ± 0.973
6.066GlyThr: 6.066 ± 0.775
5.583GlyVal: 5.583 ± 0.549
2.47GlyTrp: 2.47 ± 0.344
1.879GlyTyr: 1.879 ± 0.385
0.0GlyXaa: 0.0 ± 0.0
His
2.147HisAla: 2.147 ± 0.317
0.429HisCys: 0.429 ± 0.198
1.235HisAsp: 1.235 ± 0.244
1.127HisGlu: 1.127 ± 0.305
0.537HisPhe: 0.537 ± 0.152
1.611HisGly: 1.611 ± 0.353
0.644HisHis: 0.644 ± 0.22
1.718HisIle: 1.718 ± 0.32
1.127HisLys: 1.127 ± 0.304
1.45HisLeu: 1.45 ± 0.301
0.591HisMet: 0.591 ± 0.158
0.966HisAsn: 0.966 ± 0.2
1.396HisPro: 1.396 ± 0.245
0.805HisGln: 0.805 ± 0.212
2.04HisArg: 2.04 ± 0.41
1.127HisSer: 1.127 ± 0.231
1.45HisThr: 1.45 ± 0.332
1.611HisVal: 1.611 ± 0.356
0.537HisTrp: 0.537 ± 0.173
0.805HisTyr: 0.805 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
5.422IleAla: 5.422 ± 0.569
0.644IleCys: 0.644 ± 0.246
3.865IleAsp: 3.865 ± 0.523
3.221IleGlu: 3.221 ± 0.437
0.805IlePhe: 0.805 ± 0.242
3.704IleGly: 3.704 ± 0.528
1.396IleHis: 1.396 ± 0.308
1.664IleIle: 1.664 ± 0.336
1.02IleLys: 1.02 ± 0.254
2.201IleLeu: 2.201 ± 0.375
0.429IleMet: 0.429 ± 0.153
1.611IleAsn: 1.611 ± 0.299
3.436IlePro: 3.436 ± 0.391
1.718IleGln: 1.718 ± 0.321
2.684IleArg: 2.684 ± 0.431
2.362IleSer: 2.362 ± 0.496
3.758IleThr: 3.758 ± 0.488
2.845IleVal: 2.845 ± 0.378
0.913IleTrp: 0.913 ± 0.194
0.698IleTyr: 0.698 ± 0.177
0.0IleXaa: 0.0 ± 0.0
Lys
3.758LysAla: 3.758 ± 0.564
0.537LysCys: 0.537 ± 0.174
1.718LysAsp: 1.718 ± 0.274
1.396LysGlu: 1.396 ± 0.287
1.235LysPhe: 1.235 ± 0.235
2.577LysGly: 2.577 ± 0.37
0.913LysHis: 0.913 ± 0.22
1.02LysIle: 1.02 ± 0.241
1.718LysLys: 1.718 ± 0.383
2.362LysLeu: 2.362 ± 0.484
0.805LysMet: 0.805 ± 0.161
1.235LysAsn: 1.235 ± 0.289
2.845LysPro: 2.845 ± 0.396
1.772LysGln: 1.772 ± 0.249
2.255LysArg: 2.255 ± 0.377
2.201LysSer: 2.201 ± 0.298
2.147LysThr: 2.147 ± 0.424
2.792LysVal: 2.792 ± 0.452
0.966LysTrp: 0.966 ± 0.312
0.591LysTyr: 0.591 ± 0.192
0.0LysXaa: 0.0 ± 0.0
Leu
8.321LeuAla: 8.321 ± 0.821
0.698LeuCys: 0.698 ± 0.159
4.241LeuAsp: 4.241 ± 0.618
4.295LeuGlu: 4.295 ± 0.465
2.577LeuPhe: 2.577 ± 0.339
4.832LeuGly: 4.832 ± 0.503
1.288LeuHis: 1.288 ± 0.247
2.738LeuIle: 2.738 ± 0.438
2.416LeuLys: 2.416 ± 0.425
5.1LeuLeu: 5.1 ± 0.584
1.825LeuMet: 1.825 ± 0.293
2.792LeuAsn: 2.792 ± 0.383
4.617LeuPro: 4.617 ± 0.634
3.006LeuGln: 3.006 ± 0.428
4.724LeuArg: 4.724 ± 0.593
5.315LeuSer: 5.315 ± 0.495
5.154LeuThr: 5.154 ± 0.493
5.046LeuVal: 5.046 ± 0.541
1.288LeuTrp: 1.288 ± 0.265
2.04LeuTyr: 2.04 ± 0.334
0.0LeuXaa: 0.0 ± 0.0
Met
2.094MetAla: 2.094 ± 0.315
0.161MetCys: 0.161 ± 0.12
1.342MetAsp: 1.342 ± 0.295
1.02MetGlu: 1.02 ± 0.21
0.537MetPhe: 0.537 ± 0.195
1.664MetGly: 1.664 ± 0.243
0.107MetHis: 0.107 ± 0.077
0.859MetIle: 0.859 ± 0.227
0.591MetLys: 0.591 ± 0.203
2.255MetLeu: 2.255 ± 0.368
0.537MetMet: 0.537 ± 0.214
0.859MetAsn: 0.859 ± 0.208
1.074MetPro: 1.074 ± 0.219
0.537MetGln: 0.537 ± 0.167
1.611MetArg: 1.611 ± 0.291
2.899MetSer: 2.899 ± 0.422
2.47MetThr: 2.47 ± 0.31
1.503MetVal: 1.503 ± 0.307
0.322MetTrp: 0.322 ± 0.13
0.322MetTyr: 0.322 ± 0.109
0.0MetXaa: 0.0 ± 0.0
Asn
3.275AsnAla: 3.275 ± 0.369
0.429AsnCys: 0.429 ± 0.161
1.611AsnAsp: 1.611 ± 0.298
1.611AsnGlu: 1.611 ± 0.324
0.752AsnPhe: 0.752 ± 0.242
3.919AsnGly: 3.919 ± 0.562
0.805AsnHis: 0.805 ± 0.212
1.557AsnIle: 1.557 ± 0.47
1.02AsnLys: 1.02 ± 0.24
2.308AsnLeu: 2.308 ± 0.376
0.966AsnMet: 0.966 ± 0.186
1.879AsnAsn: 1.879 ± 0.334
3.006AsnPro: 3.006 ± 0.338
0.966AsnGln: 0.966 ± 0.314
1.986AsnArg: 1.986 ± 0.379
1.557AsnSer: 1.557 ± 0.31
1.986AsnThr: 1.986 ± 0.34
2.094AsnVal: 2.094 ± 0.346
0.698AsnTrp: 0.698 ± 0.202
0.805AsnTyr: 0.805 ± 0.192
0.0AsnXaa: 0.0 ± 0.0
Pro
5.207ProAla: 5.207 ± 0.626
0.698ProCys: 0.698 ± 0.195
4.349ProAsp: 4.349 ± 0.506
4.617ProGlu: 4.617 ± 0.447
1.825ProPhe: 1.825 ± 0.304
6.389ProGly: 6.389 ± 0.743
1.664ProHis: 1.664 ± 0.362
2.094ProIle: 2.094 ± 0.365
2.416ProLys: 2.416 ± 0.389
4.885ProLeu: 4.885 ± 0.483
1.396ProMet: 1.396 ± 0.282
1.986ProAsn: 1.986 ± 0.291
4.349ProPro: 4.349 ± 0.594
2.308ProGln: 2.308 ± 0.407
3.436ProArg: 3.436 ± 0.492
3.758ProSer: 3.758 ± 0.423
3.651ProThr: 3.651 ± 0.526
4.134ProVal: 4.134 ± 0.42
1.288ProTrp: 1.288 ± 0.272
1.342ProTyr: 1.342 ± 0.286
0.0ProXaa: 0.0 ± 0.0
Gln
4.617GlnAla: 4.617 ± 0.457
0.268GlnCys: 0.268 ± 0.147
1.342GlnAsp: 1.342 ± 0.235
1.772GlnGlu: 1.772 ± 0.287
1.235GlnPhe: 1.235 ± 0.268
2.523GlnGly: 2.523 ± 0.44
1.235GlnHis: 1.235 ± 0.215
1.879GlnIle: 1.879 ± 0.383
1.342GlnLys: 1.342 ± 0.244
2.953GlnLeu: 2.953 ± 0.407
0.429GlnMet: 0.429 ± 0.183
1.02GlnAsn: 1.02 ± 0.286
2.684GlnPro: 2.684 ± 0.42
1.181GlnGln: 1.181 ± 0.341
2.47GlnArg: 2.47 ± 0.366
2.255GlnSer: 2.255 ± 0.354
1.611GlnThr: 1.611 ± 0.241
2.631GlnVal: 2.631 ± 0.377
0.913GlnTrp: 0.913 ± 0.201
0.859GlnTyr: 0.859 ± 0.262
0.0GlnXaa: 0.0 ± 0.0
Arg
7.516ArgAla: 7.516 ± 0.662
1.074ArgCys: 1.074 ± 0.277
4.724ArgAsp: 4.724 ± 0.562
4.724ArgGlu: 4.724 ± 0.605
2.147ArgPhe: 2.147 ± 0.401
3.758ArgGly: 3.758 ± 0.416
1.718ArgHis: 1.718 ± 0.476
3.704ArgIle: 3.704 ± 0.589
2.792ArgLys: 2.792 ± 0.419
4.832ArgLeu: 4.832 ± 0.525
2.416ArgMet: 2.416 ± 0.373
2.416ArgAsn: 2.416 ± 0.426
3.49ArgPro: 3.49 ± 0.471
1.933ArgGln: 1.933 ± 0.394
6.066ArgArg: 6.066 ± 0.821
3.543ArgSer: 3.543 ± 0.428
3.865ArgThr: 3.865 ± 0.52
4.563ArgVal: 4.563 ± 0.549
2.308ArgTrp: 2.308 ± 0.368
1.825ArgTyr: 1.825 ± 0.284
0.0ArgXaa: 0.0 ± 0.0
Ser
6.281SerAla: 6.281 ± 0.881
0.591SerCys: 0.591 ± 0.226
4.456SerAsp: 4.456 ± 0.564
3.006SerGlu: 3.006 ± 0.443
1.772SerPhe: 1.772 ± 0.292
6.066SerGly: 6.066 ± 1.112
1.396SerHis: 1.396 ± 0.294
3.006SerIle: 3.006 ± 0.405
2.094SerLys: 2.094 ± 0.435
3.704SerLeu: 3.704 ± 0.397
1.45SerMet: 1.45 ± 0.262
2.04SerAsn: 2.04 ± 0.344
3.651SerPro: 3.651 ± 0.327
1.986SerGln: 1.986 ± 0.292
3.758SerArg: 3.758 ± 0.417
3.758SerSer: 3.758 ± 0.714
3.543SerThr: 3.543 ± 0.478
5.046SerVal: 5.046 ± 0.618
1.127SerTrp: 1.127 ± 0.28
1.396SerTyr: 1.396 ± 0.269
0.0SerXaa: 0.0 ± 0.0
Thr
6.335ThrAla: 6.335 ± 0.588
0.698ThrCys: 0.698 ± 0.266
4.456ThrAsp: 4.456 ± 0.492
3.919ThrGlu: 3.919 ± 0.387
1.825ThrPhe: 1.825 ± 0.28
6.281ThrGly: 6.281 ± 0.6
1.772ThrHis: 1.772 ± 0.38
3.651ThrIle: 3.651 ± 0.474
2.04ThrLys: 2.04 ± 0.394
4.563ThrLeu: 4.563 ± 0.469
1.396ThrMet: 1.396 ± 0.264
1.986ThrAsn: 1.986 ± 0.389
4.563ThrPro: 4.563 ± 0.608
2.094ThrGln: 2.094 ± 0.324
4.026ThrArg: 4.026 ± 0.539
4.026ThrSer: 4.026 ± 0.542
4.295ThrThr: 4.295 ± 0.636
5.637ThrVal: 5.637 ± 0.596
1.181ThrTrp: 1.181 ± 0.268
1.342ThrTyr: 1.342 ± 0.261
0.0ThrXaa: 0.0 ± 0.0
Val
6.55ValAla: 6.55 ± 0.694
1.181ValCys: 1.181 ± 0.229
4.939ValAsp: 4.939 ± 0.447
4.563ValGlu: 4.563 ± 0.424
2.147ValPhe: 2.147 ± 0.351
5.476ValGly: 5.476 ± 0.615
1.503ValHis: 1.503 ± 0.338
3.865ValIle: 3.865 ± 0.464
2.577ValLys: 2.577 ± 0.385
5.476ValLeu: 5.476 ± 0.661
1.02ValMet: 1.02 ± 0.169
2.094ValAsn: 2.094 ± 0.346
3.758ValPro: 3.758 ± 0.358
2.738ValGln: 2.738 ± 0.289
4.885ValArg: 4.885 ± 0.645
4.885ValSer: 4.885 ± 0.552
4.939ValThr: 4.939 ± 0.559
6.228ValVal: 6.228 ± 0.703
2.04ValTrp: 2.04 ± 0.325
1.235ValTyr: 1.235 ± 0.272
0.0ValXaa: 0.0 ± 0.0
Trp
2.04TrpAla: 2.04 ± 0.392
0.322TrpCys: 0.322 ± 0.12
1.288TrpAsp: 1.288 ± 0.26
1.074TrpGlu: 1.074 ± 0.348
0.752TrpPhe: 0.752 ± 0.234
1.235TrpGly: 1.235 ± 0.277
0.483TrpHis: 0.483 ± 0.165
1.181TrpIle: 1.181 ± 0.232
0.752TrpLys: 0.752 ± 0.168
1.557TrpLeu: 1.557 ± 0.321
1.02TrpMet: 1.02 ± 0.232
0.644TrpAsn: 0.644 ± 0.268
1.235TrpPro: 1.235 ± 0.302
1.074TrpGln: 1.074 ± 0.238
2.47TrpArg: 2.47 ± 0.458
1.342TrpSer: 1.342 ± 0.268
1.986TrpThr: 1.986 ± 0.33
1.718TrpVal: 1.718 ± 0.432
1.127TrpTrp: 1.127 ± 0.241
0.537TrpTyr: 0.537 ± 0.177
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.201TyrAla: 2.201 ± 0.344
0.322TyrCys: 0.322 ± 0.14
1.557TyrAsp: 1.557 ± 0.357
2.04TyrGlu: 2.04 ± 0.343
0.805TyrPhe: 0.805 ± 0.222
1.825TyrGly: 1.825 ± 0.351
0.215TyrHis: 0.215 ± 0.091
1.235TyrIle: 1.235 ± 0.271
0.752TyrLys: 0.752 ± 0.227
1.45TyrLeu: 1.45 ± 0.302
0.161TyrMet: 0.161 ± 0.079
0.698TyrAsn: 0.698 ± 0.192
1.557TyrPro: 1.557 ± 0.246
0.752TyrGln: 0.752 ± 0.195
1.611TyrArg: 1.611 ± 0.311
0.859TyrSer: 0.859 ± 0.216
1.664TyrThr: 1.664 ± 0.326
2.255TyrVal: 2.255 ± 0.258
0.537TyrTrp: 0.537 ± 0.163
0.591TyrTyr: 0.591 ± 0.182
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (18628 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski