Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Corynebacterium; Corynebacterium efficiens

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2946 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8FRX4|Q8FRX4_COREF Putative vanillate O-demethylase oxygenase subunit A OS=Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) OX=196164 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 5.23KK3 pKa = 10.41FFAAGVGRR11 pKa = 11.84TLGIVGAVALTTAGLVACSSPPNEE35 pKa = 4.69NPSPCNDD42 pKa = 3.26QTPQDD47 pKa = 3.68TAFAKK52 pKa = 10.84YY53 pKa = 9.98LDD55 pKa = 4.14AGEE58 pKa = 4.55IPTTGDD64 pKa = 3.03EE65 pKa = 4.16PFRR68 pKa = 11.84FEE70 pKa = 4.39VTDD73 pKa = 3.49NHH75 pKa = 6.89YY76 pKa = 10.3DD77 pKa = 3.54ACDD80 pKa = 4.09HH81 pKa = 6.55ISWLGLTGTTQPEE94 pKa = 4.58GEE96 pKa = 4.34EE97 pKa = 4.12DD98 pKa = 3.17TSGFVVFFQDD108 pKa = 3.27GDD110 pKa = 4.04LVTDD114 pKa = 4.13PRR116 pKa = 11.84PVQMGSAPFVDD127 pKa = 5.47RR128 pKa = 11.84NDD130 pKa = 4.53ADD132 pKa = 3.74DD133 pKa = 3.55QAIVQFSHH141 pKa = 6.22YY142 pKa = 9.86APPGEE147 pKa = 4.15ATTTEE152 pKa = 3.76LRR154 pKa = 11.84EE155 pKa = 4.08EE156 pKa = 3.86IFNYY160 pKa = 9.96VDD162 pKa = 3.36GKK164 pKa = 10.79IEE166 pKa = 4.1VEE168 pKa = 4.54DD169 pKa = 3.49EE170 pKa = 3.88AAYY173 pKa = 10.32EE174 pKa = 4.54DD175 pKa = 4.47YY176 pKa = 11.33AQGRR180 pKa = 11.84NQLVMQAGEE189 pKa = 4.26VCSVEE194 pKa = 4.65SEE196 pKa = 4.5DD197 pKa = 4.25PGEE200 pKa = 4.51SGDD203 pKa = 4.02AAGAGAAACC212 pKa = 4.47

Molecular weight: 22.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8FSH0|GPMA_COREF 2 3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) OX=196164 GN=gpmA PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.63KK15 pKa = 9.39KK16 pKa = 9.04HH17 pKa = 5.39RR18 pKa = 11.84KK19 pKa = 5.37MLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2945

1

2946

959932

33

3022

325.8

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.952 ± 0.049

0.712 ± 0.013

6.1 ± 0.041

6.121 ± 0.05

3.245 ± 0.03

8.645 ± 0.041

2.289 ± 0.022

5.235 ± 0.033

2.619 ± 0.037

9.648 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.019

2.723 ± 0.024

5.397 ± 0.035

3.203 ± 0.025

6.663 ± 0.043

5.631 ± 0.03

6.633 ± 0.036

8.277 ± 0.042

1.452 ± 0.021

2.127 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski