Amino acid dipepetide frequency for Saccharothrix sp. ST-888

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.87AlaAla: 22.87 ± 0.18	1.049AlaCys: 1.049 ± 0.024	8.604AlaAsp: 8.604 ± 0.098	9.628AlaGlu: 9.628 ± 0.138	3.549AlaPhe: 3.549 ± 0.046	13.473AlaGly: 13.473 ± 0.11	2.683AlaHis: 2.683 ± 0.04	3.635AlaIle: 3.635 ± 0.051	2.935AlaLys: 2.935 ± 0.057	14.842AlaLeu: 14.842 ± 0.142	2.496AlaMet: 2.496 ± 0.039	2.104AlaAsn: 2.104 ± 0.036	7.169AlaPro: 7.169 ± 0.089	4.136AlaGln: 4.136 ± 0.051	9.617AlaArg: 9.617 ± 0.094	5.605AlaSer: 5.605 ± 0.074	7.054AlaThr: 7.054 ± 0.074	12.812AlaVal: 12.812 ± 0.102	1.762AlaTrp: 1.762 ± 0.033	2.55AlaTyr: 2.55 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.047CysAla: 1.047 ± 0.024	0.094CysCys: 0.094 ± 0.007	0.457CysAsp: 0.457 ± 0.016	0.374CysGlu: 0.374 ± 0.014	0.231CysPhe: 0.231 ± 0.013	0.994CysGly: 0.994 ± 0.025	0.203CysHis: 0.203 ± 0.01	0.206CysIle: 0.206 ± 0.012	0.124CysLys: 0.124 ± 0.008	0.776CysLeu: 0.776 ± 0.024	0.119CysMet: 0.119 ± 0.008	0.142CysAsn: 0.142 ± 0.01	0.498CysPro: 0.498 ± 0.02	0.19CysGln: 0.19 ± 0.011	0.664CysArg: 0.664 ± 0.018	0.507CysSer: 0.507 ± 0.019	0.571CysThr: 0.571 ± 0.018	0.557CysVal: 0.557 ± 0.021	0.139CysTrp: 0.139 ± 0.011	0.169CysTyr: 0.169 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.085AspAla: 7.085 ± 0.064	0.429AspCys: 0.429 ± 0.017	2.895AspAsp: 2.895 ± 0.048	3.572AspGlu: 3.572 ± 0.051	1.629AspPhe: 1.629 ± 0.033	6.147AspGly: 6.147 ± 0.071	1.403AspHis: 1.403 ± 0.031	1.725AspIle: 1.725 ± 0.031	0.987AspLys: 0.987 ± 0.029	6.365AspLeu: 6.365 ± 0.075	0.66AspMet: 0.66 ± 0.019	0.926AspAsn: 0.926 ± 0.026	4.497AspPro: 4.497 ± 0.055	1.742AspGln: 1.742 ± 0.029	5.026AspArg: 5.026 ± 0.055	2.668AspSer: 2.668 ± 0.039	2.926AspThr: 2.926 ± 0.044	4.127AspVal: 4.127 ± 0.052	1.002AspTrp: 1.002 ± 0.024	1.185AspTyr: 1.185 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	7.339GluAla: 7.339 ± 0.104	0.392GluCys: 0.392 ± 0.015	2.629GluAsp: 2.629 ± 0.047	3.23GluGlu: 3.23 ± 0.063	1.557GluPhe: 1.557 ± 0.031	4.016GluGly: 4.016 ± 0.058	1.641GluHis: 1.641 ± 0.035	2.315GluIle: 2.315 ± 0.039	1.183GluLys: 1.183 ± 0.036	7.873GluLeu: 7.873 ± 0.091	0.769GluMet: 0.769 ± 0.023	0.969GluAsn: 0.969 ± 0.025	3.215GluPro: 3.215 ± 0.054	2.738GluGln: 2.738 ± 0.041	5.218GluArg: 5.218 ± 0.06	2.572GluSer: 2.572 ± 0.043	2.653GluThr: 2.653 ± 0.044	4.738GluVal: 4.738 ± 0.061	0.725GluTrp: 0.725 ± 0.021	1.188GluTyr: 1.188 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	3.799PheAla: 3.799 ± 0.051	0.275PheCys: 0.275 ± 0.013	2.001PheAsp: 2.001 ± 0.035	1.507PheGlu: 1.507 ± 0.032	0.879PhePhe: 0.879 ± 0.027	3.129PheGly: 3.129 ± 0.05	0.655PheHis: 0.655 ± 0.018	0.731PheIle: 0.731 ± 0.023	0.493PheLys: 0.493 ± 0.02	2.59PheLeu: 2.59 ± 0.043	0.371PheMet: 0.371 ± 0.014	0.593PheAsn: 0.593 ± 0.02	1.483PhePro: 1.483 ± 0.033	0.764PheGln: 0.764 ± 0.025	1.817PheArg: 1.817 ± 0.038	1.473PheSer: 1.473 ± 0.036	1.942PheThr: 1.942 ± 0.033	2.117PheVal: 2.117 ± 0.033	0.414PheTrp: 0.414 ± 0.017	0.536PheTyr: 0.536 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.023GlyAla: 11.023 ± 0.086	0.856GlyCys: 0.856 ± 0.023	4.692GlyAsp: 4.692 ± 0.054	5.225GlyGlu: 5.225 ± 0.066	2.929GlyPhe: 2.929 ± 0.042	8.805GlyGly: 8.805 ± 0.11	2.357GlyHis: 2.357 ± 0.042	3.448GlyIle: 3.448 ± 0.049	2.211GlyLys: 2.211 ± 0.044	9.988GlyLeu: 9.988 ± 0.098	1.975GlyMet: 1.975 ± 0.033	1.754GlyAsn: 1.754 ± 0.035	5.267GlyPro: 5.267 ± 0.064	2.936GlyGln: 2.936 ± 0.045	7.844GlyArg: 7.844 ± 0.071	5.694GlySer: 5.694 ± 0.069	6.266GlyThr: 6.266 ± 0.069	7.506GlyVal: 7.506 ± 0.076	1.72GlyTrp: 1.72 ± 0.034	2.347GlyTyr: 2.347 ± 0.036	0.0GlyXaa: 0.0 ± 0.0
His	2.589HisAla: 2.589 ± 0.042	0.246HisCys: 0.246 ± 0.014	1.206HisAsp: 1.206 ± 0.027	1.11HisGlu: 1.11 ± 0.031	0.666HisPhe: 0.666 ± 0.018	2.421HisGly: 2.421 ± 0.033	0.677HisHis: 0.677 ± 0.022	0.641HisIle: 0.641 ± 0.018	0.306HisLys: 0.306 ± 0.014	2.553HisLeu: 2.553 ± 0.041	0.322HisMet: 0.322 ± 0.014	0.376HisAsn: 0.376 ± 0.017	1.903HisPro: 1.903 ± 0.035	0.752HisGln: 0.752 ± 0.023	2.179HisArg: 2.179 ± 0.034	1.126HisSer: 1.126 ± 0.027	1.246HisThr: 1.246 ± 0.028	1.517HisVal: 1.517 ± 0.031	0.39HisTrp: 0.39 ± 0.016	0.464HisTyr: 0.464 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.96IleAla: 4.96 ± 0.061	0.314IleCys: 0.314 ± 0.014	2.158IleAsp: 2.158 ± 0.037	2.084IleGlu: 2.084 ± 0.036	0.705IlePhe: 0.705 ± 0.024	3.689IleGly: 3.689 ± 0.057	0.642IleHis: 0.642 ± 0.02	0.889IleIle: 0.889 ± 0.026	0.722IleLys: 0.722 ± 0.024	2.427IleLeu: 2.427 ± 0.041	0.396IleMet: 0.396 ± 0.016	0.754IleAsn: 0.754 ± 0.024	1.812IlePro: 1.812 ± 0.033	0.786IleGln: 0.786 ± 0.022	2.334IleArg: 2.334 ± 0.039	1.837IleSer: 1.837 ± 0.036	2.226IleThr: 2.226 ± 0.036	2.563IleVal: 2.563 ± 0.044	0.365IleTrp: 0.365 ± 0.016	0.514IleTyr: 0.514 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	2.75LysAla: 2.75 ± 0.055	0.093LysCys: 0.093 ± 0.007	1.157LysAsp: 1.157 ± 0.031	1.01LysGlu: 1.01 ± 0.029	0.435LysPhe: 0.435 ± 0.017	1.638LysGly: 1.638 ± 0.041	0.422LysHis: 0.422 ± 0.015	0.794LysIle: 0.794 ± 0.026	0.621LysLys: 0.621 ± 0.025	2.007LysLeu: 2.007 ± 0.044	0.361LysMet: 0.361 ± 0.016	0.453LysAsn: 0.453 ± 0.019	1.237LysPro: 1.237 ± 0.03	0.673LysGln: 0.673 ± 0.023	1.243LysArg: 1.243 ± 0.03	1.081LysSer: 1.081 ± 0.031	1.159LysThr: 1.159 ± 0.032	1.76LysVal: 1.76 ± 0.04	0.238LysTrp: 0.238 ± 0.012	0.447LysTyr: 0.447 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	16.636LeuAla: 16.636 ± 0.148	0.846LeuCys: 0.846 ± 0.023	6.982LeuAsp: 6.982 ± 0.071	4.874LeuGlu: 4.874 ± 0.062	2.723LeuPhe: 2.723 ± 0.043	9.81LeuGly: 9.81 ± 0.085	2.455LeuHis: 2.455 ± 0.044	3.407LeuIle: 3.407 ± 0.058	1.901LeuLys: 1.901 ± 0.036	11.995LeuLeu: 11.995 ± 0.146	1.587LeuMet: 1.587 ± 0.03	1.763LeuAsn: 1.763 ± 0.03	7.002LeuPro: 7.002 ± 0.076	2.481LeuGln: 2.481 ± 0.039	8.934LeuArg: 8.934 ± 0.096	5.427LeuSer: 5.427 ± 0.058	6.977LeuThr: 6.977 ± 0.072	9.014LeuVal: 9.014 ± 0.085	1.316LeuTrp: 1.316 ± 0.03	1.777LeuTyr: 1.777 ± 0.034	0.0LeuXaa: 0.0 ± 0.0
Met	2.223MetAla: 2.223 ± 0.04	0.122MetCys: 0.122 ± 0.008	0.868MetAsp: 0.868 ± 0.024	0.647MetGlu: 0.647 ± 0.02	0.435MetPhe: 0.435 ± 0.018	1.204MetGly: 1.204 ± 0.028	0.354MetHis: 0.354 ± 0.016	0.626MetIle: 0.626 ± 0.02	0.348MetLys: 0.348 ± 0.015	1.73MetLeu: 1.73 ± 0.035	0.27MetMet: 0.27 ± 0.014	0.378MetAsn: 0.378 ± 0.015	1.06MetPro: 1.06 ± 0.025	0.482MetGln: 0.482 ± 0.016	1.225MetArg: 1.225 ± 0.027	1.213MetSer: 1.213 ± 0.026	1.377MetThr: 1.377 ± 0.029	1.331MetVal: 1.331 ± 0.028	0.174MetTrp: 0.174 ± 0.01	0.302MetTyr: 0.302 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.186AsnAla: 2.186 ± 0.04	0.173AsnCys: 0.173 ± 0.009	0.893AsnAsp: 0.893 ± 0.025	0.801AsnGlu: 0.801 ± 0.024	0.489AsnPhe: 0.489 ± 0.019	2.015AsnGly: 2.015 ± 0.041	0.402AsnHis: 0.402 ± 0.017	0.655AsnIle: 0.655 ± 0.019	0.363AsnLys: 0.363 ± 0.015	1.847AsnLeu: 1.847 ± 0.039	0.319AsnMet: 0.319 ± 0.013	0.452AsnAsn: 0.452 ± 0.028	1.414AsnPro: 1.414 ± 0.032	0.554AsnGln: 0.554 ± 0.022	1.317AsnArg: 1.317 ± 0.028	1.044AsnSer: 1.044 ± 0.027	1.121AsnThr: 1.121 ± 0.03	1.304AsnVal: 1.304 ± 0.028	0.278AsnTrp: 0.278 ± 0.012	0.424AsnTyr: 0.424 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.19ProAla: 9.19 ± 0.095	0.362ProCys: 0.362 ± 0.016	4.215ProAsp: 4.215 ± 0.052	4.442ProGlu: 4.442 ± 0.057	1.57ProPhe: 1.57 ± 0.03	6.764ProGly: 6.764 ± 0.072	1.262ProHis: 1.262 ± 0.03	1.547ProIle: 1.547 ± 0.032	1.126ProLys: 1.126 ± 0.031	5.452ProLeu: 5.452 ± 0.062	0.923ProMet: 0.923 ± 0.027	1.013ProAsn: 1.013 ± 0.024	3.277ProPro: 3.277 ± 0.067	2.015ProGln: 2.015 ± 0.042	3.606ProArg: 3.606 ± 0.054	3.219ProSer: 3.219 ± 0.058	3.367ProThr: 3.367 ± 0.039	5.586ProVal: 5.586 ± 0.063	0.906ProTrp: 0.906 ± 0.023	1.358ProTyr: 1.358 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	4.293GlnAla: 4.293 ± 0.057	0.18GlnCys: 0.18 ± 0.01	1.529GlnAsp: 1.529 ± 0.033	1.477GlnGlu: 1.477 ± 0.031	0.723GlnPhe: 0.723 ± 0.022	2.568GlnGly: 2.568 ± 0.049	0.752GlnHis: 0.752 ± 0.021	1.121GlnIle: 1.121 ± 0.026	0.554GlnLys: 0.554 ± 0.021	3.754GlnLeu: 3.754 ± 0.048	0.452GlnMet: 0.452 ± 0.016	0.571GlnAsn: 0.571 ± 0.019	1.983GlnPro: 1.983 ± 0.042	1.561GlnGln: 1.561 ± 0.042	2.577GlnArg: 2.577 ± 0.043	1.428GlnSer: 1.428 ± 0.028	1.423GlnThr: 1.423 ± 0.032	2.749GlnVal: 2.749 ± 0.047	0.45GlnTrp: 0.45 ± 0.017	0.651GlnTyr: 0.651 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	9.526ArgAla: 9.526 ± 0.089	0.664ArgCys: 0.664 ± 0.019	3.724ArgAsp: 3.724 ± 0.048	4.606ArgGlu: 4.606 ± 0.061	2.274ArgPhe: 2.274 ± 0.04	5.407ArgGly: 5.407 ± 0.054	1.973ArgHis: 1.973 ± 0.039	3.366ArgIle: 3.366 ± 0.044	1.454ArgLys: 1.454 ± 0.034	9.173ArgLeu: 9.173 ± 0.089	1.614ArgMet: 1.614 ± 0.03	1.454ArgAsn: 1.454 ± 0.027	5.01ArgPro: 5.01 ± 0.071	2.554ArgGln: 2.554 ± 0.039	7.446ArgArg: 7.446 ± 0.09	4.316ArgSer: 4.316 ± 0.052	5.171ArgThr: 5.171 ± 0.063	5.488ArgVal: 5.488 ± 0.061	1.306ArgTrp: 1.306 ± 0.027	1.836ArgTyr: 1.836 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	7.133SerAla: 7.133 ± 0.08	0.472SerCys: 0.472 ± 0.017	2.554SerAsp: 2.554 ± 0.042	2.367SerGlu: 2.367 ± 0.041	1.643SerPhe: 1.643 ± 0.034	6.075SerGly: 6.075 ± 0.071	1.056SerHis: 1.056 ± 0.025	1.557SerIle: 1.557 ± 0.033	0.923SerLys: 0.923 ± 0.027	4.891SerLeu: 4.891 ± 0.058	1.028SerMet: 1.028 ± 0.025	0.94SerAsn: 0.94 ± 0.025	3.256SerPro: 3.256 ± 0.054	1.343SerGln: 1.343 ± 0.03	3.584SerArg: 3.584 ± 0.049	3.053SerSer: 3.053 ± 0.059	3.139SerThr: 3.139 ± 0.042	4.165SerVal: 4.165 ± 0.057	0.98SerTrp: 0.98 ± 0.029	1.263SerTyr: 1.263 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	9.011ThrAla: 9.011 ± 0.085	0.419ThrCys: 0.419 ± 0.016	3.455ThrAsp: 3.455 ± 0.051	3.192ThrGlu: 3.192 ± 0.046	1.638ThrPhe: 1.638 ± 0.033	6.79ThrGly: 6.79 ± 0.078	1.017ThrHis: 1.017 ± 0.024	1.65ThrIle: 1.65 ± 0.033	1.061ThrLys: 1.061 ± 0.03	5.658ThrLeu: 5.658 ± 0.062	0.906ThrMet: 0.906 ± 0.023	0.995ThrAsn: 0.995 ± 0.026	4.015ThrPro: 4.015 ± 0.052	1.28ThrGln: 1.28 ± 0.031	3.701ThrArg: 3.701 ± 0.041	2.953ThrSer: 2.953 ± 0.053	3.588ThrThr: 3.588 ± 0.059	6.173ThrVal: 6.173 ± 0.071	0.824ThrTrp: 0.824 ± 0.02	1.212ThrTyr: 1.212 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.705ValAla: 10.705 ± 0.088	0.733ValCys: 0.733 ± 0.023	4.934ValAsp: 4.934 ± 0.049	4.962ValGlu: 4.962 ± 0.067	2.396ValPhe: 2.396 ± 0.04	6.64ValGly: 6.64 ± 0.071	1.951ValHis: 1.951 ± 0.029	2.871ValIle: 2.871 ± 0.044	1.604ValLys: 1.604 ± 0.039	9.818ValLeu: 9.818 ± 0.092	1.327ValMet: 1.327 ± 0.033	1.678ValAsn: 1.678 ± 0.038	5.152ValPro: 5.152 ± 0.061	2.33ValGln: 2.33 ± 0.039	6.89ValArg: 6.89 ± 0.069	4.275ValSer: 4.275 ± 0.066	5.224ValThr: 5.224 ± 0.062	7.738ValVal: 7.738 ± 0.082	0.987ValTrp: 0.987 ± 0.024	1.497ValTyr: 1.497 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.629TrpAla: 1.629 ± 0.029	0.149TrpCys: 0.149 ± 0.01	0.755TrpAsp: 0.755 ± 0.023	0.714TrpGlu: 0.714 ± 0.022	0.453TrpPhe: 0.453 ± 0.018	1.034TrpGly: 1.034 ± 0.026	0.365TrpHis: 0.365 ± 0.015	0.524TrpIle: 0.524 ± 0.017	0.326TrpLys: 0.326 ± 0.015	1.816TrpLeu: 1.816 ± 0.031	0.257TrpMet: 0.257 ± 0.012	0.349TrpAsn: 0.349 ± 0.014	0.817TrpPro: 0.817 ± 0.02	0.717TrpGln: 0.717 ± 0.019	1.236TrpArg: 1.236 ± 0.026	0.918TrpSer: 0.918 ± 0.023	0.968TrpThr: 0.968 ± 0.024	0.978TrpVal: 0.978 ± 0.024	0.318TrpTrp: 0.318 ± 0.013	0.36TrpTyr: 0.36 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.581TyrAla: 2.581 ± 0.04	0.195TyrCys: 0.195 ± 0.011	1.288TyrAsp: 1.288 ± 0.033	1.138TyrGlu: 1.138 ± 0.027	0.647TyrPhe: 0.647 ± 0.022	2.225TyrGly: 2.225 ± 0.038	0.42TyrHis: 0.42 ± 0.014	0.47TyrIle: 0.47 ± 0.017	0.339TyrLys: 0.339 ± 0.016	2.352TyrLeu: 2.352 ± 0.039	0.218TyrMet: 0.218 ± 0.011	0.387TyrAsn: 0.387 ± 0.015	1.131TyrPro: 1.131 ± 0.03	0.759TyrGln: 0.759 ± 0.023	1.906TyrArg: 1.906 ± 0.028	1.013TyrSer: 1.013 ± 0.029	1.141TyrThr: 1.141 ± 0.023	1.486TyrVal: 1.486 ± 0.029	0.387TyrTrp: 0.387 ± 0.013	0.429TyrTyr: 0.429 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5936 proteins (1759706 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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