Erysipelotrichaceae bacterium MTC7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; unclassified Erysipelotrichaceae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2314 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C0BXR6|A0A1C0BXR6_9FIRM DNA methyltransferase OS=Erysipelotrichaceae bacterium MTC7 OX=1768196 GN=A4S06_06350 PE=3 SV=1
MM1 pKa = 6.18THH3 pKa = 6.34EE4 pKa = 5.21EE5 pKa = 4.22IVTMLEE11 pKa = 4.0EE12 pKa = 4.68ANLPLAYY19 pKa = 10.34DD20 pKa = 3.92HH21 pKa = 6.67FAEE24 pKa = 5.4GEE26 pKa = 4.4SPDD29 pKa = 3.8PPFLIFLFPGTDD41 pKa = 4.02NMFADD46 pKa = 3.47NKK48 pKa = 9.52VWQKK52 pKa = 10.86INQLNIEE59 pKa = 4.9LYY61 pKa = 9.55TDD63 pKa = 3.51EE64 pKa = 5.33KK65 pKa = 11.26SPEE68 pKa = 4.06TEE70 pKa = 3.81EE71 pKa = 4.21TIEE74 pKa = 5.52DD75 pKa = 3.6ILDD78 pKa = 3.83SYY80 pKa = 9.88EE81 pKa = 3.81IPYY84 pKa = 9.98EE85 pKa = 4.12KK86 pKa = 10.17SEE88 pKa = 3.94YY89 pKa = 9.42WIEE92 pKa = 4.05SEE94 pKa = 4.93KK95 pKa = 10.34MYY97 pKa = 10.91EE98 pKa = 3.82VLYY101 pKa = 8.99QTEE104 pKa = 4.2ILGGNN109 pKa = 3.59

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C0BTH9|A0A1C0BTH9_9FIRM Nitroreductase OS=Erysipelotrichaceae bacterium MTC7 OX=1768196 GN=A4S06_03890 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 9.22RR10 pKa = 11.84KK11 pKa = 8.88HH12 pKa = 5.41QKK14 pKa = 6.93THH16 pKa = 4.85GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 9.78TPGGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.59GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2314

0

2314

729312

37

2547

315.2

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.043 ± 0.052

1.092 ± 0.02

6.091 ± 0.042

7.032 ± 0.057

4.265 ± 0.041

6.325 ± 0.049

2.014 ± 0.024

7.715 ± 0.049

7.355 ± 0.055

9.07 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.956 ± 0.026

4.912 ± 0.046

3.044 ± 0.023

3.707 ± 0.03

3.683 ± 0.037

5.835 ± 0.041

5.563 ± 0.058

7.257 ± 0.047

0.782 ± 0.017

4.259 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski