Changjiang tombus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFP0|A0A1L3KFP0_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 1 OX=1922802 PE=4 SV=1
MM1 pKa = 7.69GEE3 pKa = 4.4FVCDD7 pKa = 5.47DD8 pKa = 3.71IMTTHH13 pKa = 6.42NNSIANLIRR22 pKa = 11.84GVGEE26 pKa = 3.52RR27 pKa = 11.84VLFTDD32 pKa = 4.64RR33 pKa = 11.84QLTPCIKK40 pKa = 9.61PLVGIFSKK48 pKa = 10.76RR49 pKa = 11.84CASFSRR55 pKa = 11.84EE56 pKa = 3.59VARR59 pKa = 11.84CVGRR63 pKa = 11.84QSPVSRR69 pKa = 11.84QSFVDD74 pKa = 3.67YY75 pKa = 10.84YY76 pKa = 10.35KK77 pKa = 10.77GRR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84TIYY84 pKa = 9.8QQAADD89 pKa = 4.16GLVLKK94 pKa = 9.47PIRR97 pKa = 11.84PRR99 pKa = 11.84DD100 pKa = 3.56AYY102 pKa = 11.23LSTFVKK108 pKa = 10.54AEE110 pKa = 4.34KK111 pKa = 10.19INLSNKK117 pKa = 8.83PDD119 pKa = 4.04PAPRR123 pKa = 11.84VIQPRR128 pKa = 11.84NPRR131 pKa = 11.84YY132 pKa = 9.44NVEE135 pKa = 4.17LGRR138 pKa = 11.84FLLPLEE144 pKa = 4.57HH145 pKa = 6.72KK146 pKa = 10.79VYY148 pKa = 10.89DD149 pKa = 4.97AIDD152 pKa = 3.68EE153 pKa = 4.59VFGSPTIMSKK163 pKa = 10.97YY164 pKa = 10.13NSVDD168 pKa = 2.95QAAVLRR174 pKa = 11.84EE175 pKa = 3.74KK176 pKa = 9.71WDD178 pKa = 3.38KK179 pKa = 10.63FRR181 pKa = 11.84EE182 pKa = 4.29PVCVGLDD189 pKa = 3.18ASRR192 pKa = 11.84FDD194 pKa = 3.47QHH196 pKa = 8.35VSEE199 pKa = 4.21QALKK203 pKa = 10.63FEE205 pKa = 4.79HH206 pKa = 7.12DD207 pKa = 4.57FYY209 pKa = 11.85RR210 pKa = 11.84MVFGPRR216 pKa = 11.84KK217 pKa = 9.3DD218 pKa = 4.04LSMLLSWQLTNKK230 pKa = 9.64GFARR234 pKa = 11.84ATDD237 pKa = 3.93GSFSYY242 pKa = 10.15IRR244 pKa = 11.84KK245 pKa = 8.1GSRR248 pKa = 11.84MSGDD252 pKa = 3.39MNTSLGNKK260 pKa = 9.47FLMCAMGKK268 pKa = 8.24TYY270 pKa = 10.86LDD272 pKa = 3.34QLQIKK277 pKa = 9.93YY278 pKa = 10.16EE279 pKa = 4.0FANNGDD285 pKa = 3.68DD286 pKa = 3.3CLVFIEE292 pKa = 4.79RR293 pKa = 11.84SNLKK297 pKa = 10.09KK298 pKa = 10.86LSGLQTYY305 pKa = 9.45FRR307 pKa = 11.84GFGFKK312 pKa = 9.81IVTEE316 pKa = 4.1KK317 pKa = 10.74PVFEE321 pKa = 4.46FEE323 pKa = 4.51HH324 pKa = 8.62VEE326 pKa = 4.36FCQCRR331 pKa = 11.84PLFCNNIWRR340 pKa = 11.84MVRR343 pKa = 11.84NVKK346 pKa = 8.7TCLLKK351 pKa = 10.93DD352 pKa = 3.57VTSVNLGHH360 pKa = 7.74DD361 pKa = 3.83VTQYY365 pKa = 10.82RR366 pKa = 11.84AWLADD371 pKa = 3.06IAGCGLSFAADD382 pKa = 3.65VPILGAYY389 pKa = 9.86YY390 pKa = 10.97RR391 pKa = 11.84MLQRR395 pKa = 11.84FGQAGRR401 pKa = 11.84YY402 pKa = 9.2HH403 pKa = 7.22GDD405 pKa = 2.98DD406 pKa = 4.18SMFNCYY412 pKa = 7.93RR413 pKa = 11.84TLSKK417 pKa = 10.76NSRR420 pKa = 11.84ISSTVPDD427 pKa = 3.6ALGRR431 pKa = 11.84YY432 pKa = 7.8SFWLQTGIHH441 pKa = 7.29PDD443 pKa = 3.29GQQAIEE449 pKa = 4.34NYY451 pKa = 9.84FEE453 pKa = 3.76EE454 pKa = 5.64AIWGGDD460 pKa = 2.94KK461 pKa = 10.66RR462 pKa = 11.84QLINNIHH469 pKa = 6.49YY470 pKa = 10.04ILKK473 pKa = 10.54NGSS476 pKa = 3.07

Molecular weight: 54.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFP0|A0A1L3KFP0_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 1 OX=1922802 PE=4 SV=1
MM1 pKa = 7.2ARR3 pKa = 11.84KK4 pKa = 8.28RR5 pKa = 11.84TRR7 pKa = 11.84SSQSSDD13 pKa = 2.83EE14 pKa = 4.28SGSLISRR21 pKa = 11.84NRR23 pKa = 11.84QPPRR27 pKa = 11.84PRR29 pKa = 11.84VRR31 pKa = 11.84GSLGTNTILRR41 pKa = 11.84GVEE44 pKa = 3.56IGGSLVTDD52 pKa = 4.38ANGSAAGVYY61 pKa = 9.59PLIAGSLTGLTNSPINNIAKK81 pKa = 9.54YY82 pKa = 9.22YY83 pKa = 10.71NSFVYY88 pKa = 10.08QSAVMHH94 pKa = 6.39YY95 pKa = 9.24IPAVGLTTPGQVTAVFINNTEE116 pKa = 3.78WMSYY120 pKa = 11.0ALDD123 pKa = 3.47GTRR126 pKa = 11.84TVTEE130 pKa = 4.78LGTLALSQSNAATHH144 pKa = 6.05AVWHH148 pKa = 5.74EE149 pKa = 3.58FSYY152 pKa = 11.53AMNLPSRR159 pKa = 11.84RR160 pKa = 11.84KK161 pKa = 9.69RR162 pKa = 11.84FDD164 pKa = 3.42VNSTLPTATVDD175 pKa = 3.56VVEE178 pKa = 5.12RR179 pKa = 11.84DD180 pKa = 3.76CQGVFILFVSGAPANTTISVPRR202 pKa = 11.84RR203 pKa = 11.84SVTLQVEE210 pKa = 4.68GMSGFFPP217 pKa = 4.87

Molecular weight: 23.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

693

217

476

346.5

38.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.494 ± 0.945

2.02 ± 0.818

5.051 ± 1.199

3.896 ± 0.352

5.483 ± 0.943

7.504 ± 0.415

1.732 ± 0.183

5.051 ± 0.232

4.329 ± 1.546

8.081 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.453 ± 0.078

5.339 ± 0.342

4.473 ± 0.555

4.04 ± 0.428

7.792 ± 0.22

8.081 ± 1.563

5.772 ± 2.05

7.359 ± 0.975

1.154 ± 0.122

3.896 ± 0.352
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski