Pseudoalteromonas phage H103

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0MD13|A0A0K0MD13_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage H103 OX=1636200 PE=4 SV=1
MM1 pKa = 7.98PLTIEE6 pKa = 4.12TVKK9 pKa = 10.88AYY11 pKa = 10.39GGTGNDD17 pKa = 2.72IVIQAKK23 pKa = 9.44IDD25 pKa = 3.62AFASIYY31 pKa = 9.88TCLTSSYY38 pKa = 10.72DD39 pKa = 3.35SAIADD44 pKa = 5.08DD45 pKa = 4.08IANSYY50 pKa = 9.57IAGTLQAVSGEE61 pKa = 4.28GQVTSRR67 pKa = 11.84KK68 pKa = 9.69APNGASRR75 pKa = 11.84NFKK78 pKa = 8.81QNKK81 pKa = 8.42YY82 pKa = 9.64GDD84 pKa = 3.43SGEE87 pKa = 4.11YY88 pKa = 10.86DD89 pKa = 3.35NALLEE94 pKa = 4.31AAYY97 pKa = 9.81KK98 pKa = 10.44SDD100 pKa = 5.06ANFCLPVDD108 pKa = 3.82DD109 pKa = 5.21SYY111 pKa = 11.96FVIGTAGTVYY121 pKa = 10.36PADD124 pKa = 3.76NPQQ127 pKa = 2.74

Molecular weight: 13.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0MD19|A0A0K0MD19_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage H103 OX=1636200 PE=4 SV=1
MM1 pKa = 7.43SNAKK5 pKa = 9.5RR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.17QYY10 pKa = 7.34EE11 pKa = 3.86QRR13 pKa = 11.84LSEE16 pKa = 4.33HH17 pKa = 6.57GYY19 pKa = 10.84DD20 pKa = 3.87YY21 pKa = 11.5QLLLNRR27 pKa = 11.84KK28 pKa = 8.24LNGMKK33 pKa = 9.91FQSEE37 pKa = 3.55IRR39 pKa = 11.84GRR41 pKa = 11.84NNGFTTSSRR50 pKa = 3.52

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

13295

44

866

177.3

19.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.184 ± 0.408

1.444 ± 0.161

6.348 ± 0.216

6.672 ± 0.293

3.866 ± 0.198

7.198 ± 0.281

1.595 ± 0.151

6.258 ± 0.192

6.687 ± 0.386

7.777 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.587 ± 0.197

5.799 ± 0.333

3.407 ± 0.229

3.768 ± 0.204

3.881 ± 0.231

6.897 ± 0.292

5.92 ± 0.29

6.499 ± 0.264

1.249 ± 0.123

3.964 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski