Tetraselmis viridis virus S20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4QNZ4|M4QNZ4_9CAUD Uncharacterized protein OS=Tetraselmis viridis virus S20 OX=754070 GN=TVGG_00037 PE=4 SV=1
MM1 pKa = 7.16TLLWQDD7 pKa = 3.67GFDD10 pKa = 3.81QYY12 pKa = 12.15GLDD15 pKa = 4.06EE16 pKa = 4.66SVMLQGPYY24 pKa = 10.59AQVSGNFALSDD35 pKa = 3.57VNPRR39 pKa = 11.84TGSICMRR46 pKa = 11.84FSSSGTSILRR56 pKa = 11.84RR57 pKa = 11.84TLGVARR63 pKa = 11.84QTIGAGLAWRR73 pKa = 11.84PEE75 pKa = 4.69AIPSSNDD82 pKa = 3.31DD83 pKa = 3.01VWLVSFLDD91 pKa = 3.73NSNVAILTLEE101 pKa = 4.03VSTTGQIRR109 pKa = 11.84AKK111 pKa = 10.0GGTVTASVLGASAQNVIQAGTYY133 pKa = 6.5QHH135 pKa = 6.66IEE137 pKa = 3.64ARR139 pKa = 11.84VFSDD143 pKa = 3.62NGAGTCEE150 pKa = 3.77VRR152 pKa = 11.84VNGVTVLNLTGLSLSGDD169 pKa = 4.35PIAQLSYY176 pKa = 10.92GRR178 pKa = 11.84TFGITIEE185 pKa = 4.28ARR187 pKa = 11.84WDD189 pKa = 3.47DD190 pKa = 4.21LYY192 pKa = 11.71AWDD195 pKa = 5.14TEE197 pKa = 4.26GTEE200 pKa = 4.26NNTFLGDD207 pKa = 3.16RR208 pKa = 11.84RR209 pKa = 11.84VFTRR213 pKa = 11.84VCDD216 pKa = 3.69ADD218 pKa = 3.82GALQEE223 pKa = 4.45WARR226 pKa = 11.84STGSSTYY233 pKa = 10.55QLLDD237 pKa = 3.35EE238 pKa = 5.04TPPDD242 pKa = 3.77GDD244 pKa = 3.21TSYY247 pKa = 11.79VEE249 pKa = 4.15TSTVGDD255 pKa = 3.32IFGLTFEE262 pKa = 4.68NLPAEE267 pKa = 4.58VAGVAAVSVITYY279 pKa = 9.54ARR281 pKa = 11.84KK282 pKa = 9.75LDD284 pKa = 4.08AGPGTFQTTVVSGTAEE300 pKa = 3.85EE301 pKa = 4.55AGIEE305 pKa = 4.19HH306 pKa = 6.98PTNVTWTYY314 pKa = 10.36FQDD317 pKa = 3.59IYY319 pKa = 11.33QVDD322 pKa = 4.37PNTGSGWTRR331 pKa = 11.84ATVDD335 pKa = 3.8AIEE338 pKa = 4.24VLTEE342 pKa = 3.76RR343 pKa = 11.84TLL345 pKa = 3.51

Molecular weight: 36.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QSM1|M4QSM1_9CAUD Uncharacterized protein OS=Tetraselmis viridis virus S20 OX=754070 GN=TVGG_00006 PE=4 SV=1
MM1 pKa = 7.42TRR3 pKa = 11.84RR4 pKa = 11.84EE5 pKa = 4.24DD6 pKa = 3.22QNRR9 pKa = 11.84AHH11 pKa = 7.59DD12 pKa = 3.71ARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84AQKK19 pKa = 9.76PWRR22 pKa = 11.84KK23 pKa = 8.92WYY25 pKa = 8.46STTRR29 pKa = 11.84WRR31 pKa = 11.84RR32 pKa = 11.84LRR34 pKa = 11.84RR35 pKa = 11.84AQLRR39 pKa = 11.84RR40 pKa = 11.84QPYY43 pKa = 9.79CVICRR48 pKa = 11.84AQGKK52 pKa = 9.35SRR54 pKa = 11.84FATVADD60 pKa = 3.59HH61 pKa = 6.74VKK63 pKa = 10.34AHH65 pKa = 6.93RR66 pKa = 11.84GDD68 pKa = 3.67PHH70 pKa = 7.06LFWFGEE76 pKa = 3.94LRR78 pKa = 11.84SVCEE82 pKa = 3.65EE83 pKa = 3.52HH84 pKa = 7.31HH85 pKa = 6.84NRR87 pKa = 11.84DD88 pKa = 3.55SQIKK92 pKa = 8.28DD93 pKa = 3.13TKK95 pKa = 11.02GYY97 pKa = 10.68DD98 pKa = 3.29LTIGEE103 pKa = 5.56DD104 pKa = 4.21GFPMDD109 pKa = 4.99PNHH112 pKa = 7.23PFNIDD117 pKa = 3.02RR118 pKa = 11.84KK119 pKa = 9.83GIKK122 pKa = 9.11PP123 pKa = 3.46

Molecular weight: 14.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12499

63

941

227.3

25.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.121 ± 0.492

0.984 ± 0.142

6.689 ± 0.214

6.745 ± 0.337

3.152 ± 0.223

8.601 ± 0.312

2.24 ± 0.274

4.784 ± 0.186

3.744 ± 0.396

8.185 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.205

2.8 ± 0.173

5.272 ± 0.389

4.24 ± 0.26

7.505 ± 0.301

5.624 ± 0.249

5.92 ± 0.38

6.265 ± 0.291

2.112 ± 0.238

2.296 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski