Lichenibacterium ramalinae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Lichenibacteriaceae; Lichenibacterium

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2R9H9|A0A4Q2R9H9_9RHIZ SET domain-containing protein-lysine N-methyltransferase OS=Lichenibacterium ramalinae OX=2316527 GN=D3272_20635 PE=4 SV=1
MM1 pKa = 7.03QADD4 pKa = 3.72IKK6 pKa = 11.23YY7 pKa = 10.32LGLSEE12 pKa = 4.71LRR14 pKa = 11.84DD15 pKa = 4.43GISNGQNGSAPLASYY30 pKa = 9.96IQLAKK35 pKa = 10.62AGEE38 pKa = 4.09QFTFVVGGDD47 pKa = 3.42ASTTASVQSTLNTIGQLEE65 pKa = 4.2AAVPGSVKK73 pKa = 10.51AIEE76 pKa = 4.87GPNEE80 pKa = 3.81INNQSLTFNGVGGLQGATALQQYY103 pKa = 9.94LYY105 pKa = 8.79QTVKK109 pKa = 9.63STPALAGVSVDD120 pKa = 3.92YY121 pKa = 8.48FTGYY125 pKa = 8.36NTVGFAAGPNPSTTAGLADD144 pKa = 4.52YY145 pKa = 10.3DD146 pKa = 4.07NQHH149 pKa = 7.4PYY151 pKa = 8.94PQGGQAPASWVDD163 pKa = 3.25PAAALGNEE171 pKa = 3.91TGARR175 pKa = 11.84GKK177 pKa = 10.08FVYY180 pKa = 10.07TEE182 pKa = 3.78AGYY185 pKa = 6.94TTNLSDD191 pKa = 4.34VNGVDD196 pKa = 3.13ATVQGKK202 pKa = 7.8YY203 pKa = 8.89TLDD206 pKa = 3.79LLMDD210 pKa = 4.6AAKK213 pKa = 10.48DD214 pKa = 3.94GSSGTDD220 pKa = 3.35LYY222 pKa = 11.58QLLDD226 pKa = 3.7AYY228 pKa = 10.1KK229 pKa = 9.81PGSPQGDD236 pKa = 3.61DD237 pKa = 3.67GYY239 pKa = 12.04GLFDD243 pKa = 4.21EE244 pKa = 4.93NNNPKK249 pKa = 10.09LAATGIHH256 pKa = 6.54NLTAILADD264 pKa = 3.4KK265 pKa = 10.09GANASSFSLSVLNYY279 pKa = 9.66SVSGQPSTGNSLEE292 pKa = 4.03IGKK295 pKa = 9.81SDD297 pKa = 3.51GTTDD301 pKa = 2.97IAVWAEE307 pKa = 3.82PPLWNASTKK316 pKa = 10.64SEE318 pKa = 4.16VAAPTTTEE326 pKa = 4.01TVNLDD331 pKa = 3.19GAHH334 pKa = 5.35QVSVYY339 pKa = 11.1DD340 pKa = 4.51LLSGTAPIATYY351 pKa = 10.88ASTSSVQLQVTDD363 pKa = 3.72HH364 pKa = 6.64PLIVQVGAATSSNSSSSSSAPTVSTTPTVSTTPTVSTTPAVPTTPNVSTPTVSTTPSGSGMSSSSSTGGSTATTGSTPSSGSTTTGSGLDD454 pKa = 3.61KK455 pKa = 10.86LVLSISEE462 pKa = 4.39DD463 pKa = 3.48AYY465 pKa = 11.18QGNAQYY471 pKa = 11.1AVSVDD476 pKa = 3.31GKK478 pKa = 10.53QVTGTLTAGAAHH490 pKa = 7.23AAGADD495 pKa = 3.74DD496 pKa = 3.92TLTIYY501 pKa = 10.64GNWGAGSHH509 pKa = 5.51AVGVNFLNDD518 pKa = 3.73AYY520 pKa = 10.55AGTPSTDD527 pKa = 2.88RR528 pKa = 11.84NLYY531 pKa = 10.04VDD533 pKa = 4.27GATFDD538 pKa = 3.88GASVHH543 pKa = 6.66AGTLALLSGGMQSFNYY559 pKa = 9.86TDD561 pKa = 3.5VTPVSDD567 pKa = 3.92GGQNQSIGSGKK578 pKa = 10.01DD579 pKa = 3.21QLQLAIGEE587 pKa = 4.24DD588 pKa = 4.12AYY590 pKa = 10.22KK591 pKa = 10.98GDD593 pKa = 3.39AQYY596 pKa = 10.4TVSVDD601 pKa = 2.86GHH603 pKa = 5.06QVGGTLTAGAAQGSKK618 pKa = 10.5KK619 pKa = 10.36SDD621 pKa = 3.47LLDD624 pKa = 3.84VYY626 pKa = 10.5GNWGAGTHH634 pKa = 4.37QVAVNFLNDD643 pKa = 3.8AYY645 pKa = 10.55AGTPSTDD652 pKa = 2.83RR653 pKa = 11.84NLYY656 pKa = 10.08VNGITYY662 pKa = 10.38DD663 pKa = 3.5GSVNATSTTALLSSGSHH680 pKa = 5.6SFSFHH685 pKa = 5.18SS686 pKa = 3.73

Molecular weight: 69.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2RF91|A0A4Q2RF91_9RHIZ DUF1311 domain-containing protein OS=Lichenibacterium ramalinae OX=2316527 GN=D3272_06890 PE=4 SV=1
MM1 pKa = 7.12RR2 pKa = 11.84HH3 pKa = 4.08GRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84ARR11 pKa = 11.84APALSSPCRR20 pKa = 11.84PRR22 pKa = 11.84TSSSGPGAPGPDD34 pKa = 3.39PGASGRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84PVPIRR47 pKa = 11.84RR48 pKa = 11.84PRR50 pKa = 11.84WRR52 pKa = 11.84RR53 pKa = 11.84PRR55 pKa = 11.84AGCGSRR61 pKa = 11.84CRR63 pKa = 11.84PRR65 pKa = 11.84RR66 pKa = 11.84SSRR69 pKa = 11.84RR70 pKa = 11.84WAPNWRR76 pKa = 11.84SGPRR80 pKa = 11.84RR81 pKa = 11.84SPPRR85 pKa = 11.84GRR87 pKa = 11.84RR88 pKa = 11.84RR89 pKa = 11.84GRR91 pKa = 11.84GRR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84SS96 pKa = 3.35

Molecular weight: 11.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5222

0

5222

1654282

15

2836

316.8

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.678 ± 0.056

0.845 ± 0.01

6.007 ± 0.029

4.865 ± 0.028

3.451 ± 0.024

9.386 ± 0.028

2.069 ± 0.017

4.029 ± 0.027

2.338 ± 0.028

10.366 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.212 ± 0.014

1.914 ± 0.019

5.863 ± 0.032

2.536 ± 0.02

8.142 ± 0.039

4.868 ± 0.028

5.14 ± 0.021

8.079 ± 0.03

1.283 ± 0.014

1.929 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski