Clostridium tetani (strain Massachusetts / E88)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium tetani

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2415 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q898L8|Q898L8_CLOTE Glycerophosphoryl diester phosphodiesterase OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=CTC_00427 PE=4 SV=1
MM1 pKa = 7.3FLCFYY6 pKa = 10.91VLTKK10 pKa = 10.75LEE12 pKa = 3.82ISNNIIYY19 pKa = 10.48EE20 pKa = 3.98SDD22 pKa = 3.24NQYY25 pKa = 10.88QLPGGIYY32 pKa = 8.42MKK34 pKa = 10.29NISVIYY40 pKa = 9.79WSGTGNTEE48 pKa = 4.04AMAEE52 pKa = 3.99ALAEE56 pKa = 4.19GAKK59 pKa = 10.43AAGGQVKK66 pKa = 9.85LVSVDD71 pKa = 3.64EE72 pKa = 4.28VSEE75 pKa = 4.78DD76 pKa = 3.63DD77 pKa = 4.43VKK79 pKa = 11.45NADD82 pKa = 4.12AVALGCPSMGDD93 pKa = 3.28EE94 pKa = 4.17VLEE97 pKa = 4.47EE98 pKa = 4.52GSMEE102 pKa = 4.03PFIEE106 pKa = 4.26QTSNLYY112 pKa = 9.98KK113 pKa = 10.5GKK115 pKa = 10.04NVALFGSYY123 pKa = 9.05GWGSGQWMEE132 pKa = 4.07DD133 pKa = 2.53WQARR137 pKa = 11.84MEE139 pKa = 4.62GYY141 pKa = 9.77GANLVEE147 pKa = 5.98DD148 pKa = 4.33GLIINYY154 pKa = 8.75TPDD157 pKa = 3.82DD158 pKa = 4.23EE159 pKa = 5.58GLEE162 pKa = 4.12KK163 pKa = 10.59CKK165 pKa = 10.4QLGEE169 pKa = 4.4SLVKK173 pKa = 10.76AA174 pKa = 4.39

Molecular weight: 18.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q898L3|HIS2_CLOTE Phosphoribosyl-ATP pyrophosphatase OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=hisE PE=3 SV=1
MM1 pKa = 7.41YY2 pKa = 9.67YY3 pKa = 10.45WNMNCGDD10 pKa = 3.87CFYY13 pKa = 10.47RR14 pKa = 11.84QQEE17 pKa = 3.96AMEE20 pKa = 4.49LNRR23 pKa = 11.84RR24 pKa = 11.84WRR26 pKa = 11.84PWRR29 pKa = 11.84VISGRR34 pKa = 11.84VTSGLGVSSWAPNRR48 pKa = 11.84LDD50 pKa = 3.49LFARR54 pKa = 11.84GSNRR58 pKa = 11.84QLIHH62 pKa = 6.53KK63 pKa = 8.87WRR65 pKa = 11.84EE66 pKa = 3.35NSRR69 pKa = 11.84WSNWEE74 pKa = 3.75DD75 pKa = 3.18LGGVLTSSPSAVSWGRR91 pKa = 11.84NRR93 pKa = 11.84IDD95 pKa = 3.27VVVRR99 pKa = 11.84GTDD102 pKa = 2.63NAMYY106 pKa = 9.75HH107 pKa = 5.75KK108 pKa = 9.23WWNGSRR114 pKa = 11.84WSDD117 pKa = 3.06WEE119 pKa = 4.15SLGGVLTSAPSICSWASNRR138 pKa = 11.84LDD140 pKa = 3.49CFVRR144 pKa = 11.84GTDD147 pKa = 3.08NQLYY151 pKa = 8.96HH152 pKa = 6.61KK153 pKa = 9.05WWDD156 pKa = 3.21GSRR159 pKa = 11.84WHH161 pKa = 7.1DD162 pKa = 3.37WEE164 pKa = 5.0ALGGNLTSGPGCVSWGPNRR183 pKa = 11.84IDD185 pKa = 3.08VFARR189 pKa = 11.84GRR191 pKa = 11.84NNTLIHH197 pKa = 6.02KK198 pKa = 8.67WWDD201 pKa = 3.17GSRR204 pKa = 11.84WSDD207 pKa = 3.26WEE209 pKa = 4.13DD210 pKa = 3.18LGGNLSSAPCASSRR224 pKa = 11.84GRR226 pKa = 11.84NRR228 pKa = 11.84IDD230 pKa = 2.97VFARR234 pKa = 11.84GRR236 pKa = 11.84NNQLIYY242 pKa = 10.95KK243 pKa = 8.46NWNGSRR249 pKa = 11.84WSNWQNLGGFLTSPPVSISPTPNRR273 pKa = 11.84IEE275 pKa = 3.97VYY277 pKa = 10.17ARR279 pKa = 11.84GRR281 pKa = 11.84NGIIINRR288 pKa = 11.84NFSGG292 pKa = 3.42

Molecular weight: 33.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2415

0

2415

809352

60

2838

335.1

37.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.229 ± 0.058

1.127 ± 0.017

5.389 ± 0.037

7.644 ± 0.059

4.413 ± 0.04

6.317 ± 0.05

1.342 ± 0.019

10.456 ± 0.064

9.962 ± 0.059

9.006 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.606 ± 0.022

6.555 ± 0.052

2.746 ± 0.027

2.146 ± 0.019

3.285 ± 0.037

6.163 ± 0.033

4.673 ± 0.036

6.165 ± 0.042

0.642 ± 0.015

4.131 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski