Honeysuckle yellow vein virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8QXB8|Q8QXB8_9GEMI Transcriptional activator protein OS=Honeysuckle yellow vein virus OX=240865 GN=C2 PE=3 SV=1
MM1 pKa = 7.71APPKK5 pKa = 10.19RR6 pKa = 11.84FRR8 pKa = 11.84INSKK12 pKa = 10.54NYY14 pKa = 8.37FVTYY18 pKa = 6.97PHH20 pKa = 7.12CSLTKK25 pKa = 10.59EE26 pKa = 3.99EE27 pKa = 5.27ALTQIKK33 pKa = 10.4ALEE36 pKa = 4.34TPTNKK41 pKa = 10.07LFIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.54LHH51 pKa = 6.34EE52 pKa = 5.86DD53 pKa = 4.22GSPHH57 pKa = 6.3LHH59 pKa = 6.68ILIQFEE65 pKa = 4.5GKK67 pKa = 8.81YY68 pKa = 9.53QCTNQRR74 pKa = 11.84FFDD77 pKa = 4.07LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.34SSSDD99 pKa = 2.92VKK101 pKa = 10.47TYY103 pKa = 8.75MEE105 pKa = 5.02KK106 pKa = 10.85DD107 pKa = 2.98GDD109 pKa = 4.09IIDD112 pKa = 4.36FGVFQIDD119 pKa = 3.2GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.26AYY134 pKa = 10.9AEE136 pKa = 4.72AINSGSKK143 pKa = 10.4SSALCILRR151 pKa = 11.84EE152 pKa = 3.97KK153 pKa = 10.63APKK156 pKa = 10.5DD157 pKa = 3.42FVLQFHH163 pKa = 6.68NLNSNLDD170 pKa = 4.05RR171 pKa = 11.84IFAPPLEE178 pKa = 4.38EE179 pKa = 4.92FISPFLSSSFDD190 pKa = 3.4QVPEE194 pKa = 3.89QLEE197 pKa = 3.83EE198 pKa = 3.73WAAEE202 pKa = 4.03NVRR205 pKa = 11.84DD206 pKa = 4.51SARR209 pKa = 11.84PWRR212 pKa = 11.84PMSIVIEE219 pKa = 4.37GDD221 pKa = 3.13SRR223 pKa = 11.84TGKK226 pKa = 8.52TMWARR231 pKa = 11.84SLSPRR236 pKa = 11.84HH237 pKa = 6.09NYY239 pKa = 10.09LCGHH243 pKa = 7.39LDD245 pKa = 4.41LSPKK249 pKa = 9.97IYY251 pKa = 10.86SNEE254 pKa = 3.09AWYY257 pKa = 10.82NVIDD261 pKa = 5.29DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 10.78TEE313 pKa = 4.02YY314 pKa = 11.19LDD316 pKa = 3.6EE317 pKa = 5.81DD318 pKa = 3.79KK319 pKa = 11.41NAALKK324 pKa = 10.41AWAIKK329 pKa = 10.27NATFVTLYY337 pKa = 10.64GPLYY341 pKa = 10.67SGTHH345 pKa = 5.0QGATQIRR352 pKa = 11.84EE353 pKa = 4.29EE354 pKa = 4.11EE355 pKa = 4.32TTTQEE360 pKa = 3.52EE361 pKa = 4.57DD362 pKa = 2.98

Molecular weight: 41.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8QXC1|Q8QXC1_9GEMI Protein V2 OS=Honeysuckle yellow vein virus OX=240865 GN=V2 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.31RR4 pKa = 11.84PADD7 pKa = 3.24IGIFTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.03SPYY27 pKa = 10.83ANRR30 pKa = 11.84ASVPIALATNKK41 pKa = 9.36RR42 pKa = 11.84RR43 pKa = 11.84SWAQRR48 pKa = 11.84PMYY51 pKa = 9.7RR52 pKa = 11.84KK53 pKa = 8.22PRR55 pKa = 11.84MYY57 pKa = 10.82RR58 pKa = 11.84MYY60 pKa = 10.57RR61 pKa = 11.84SPDD64 pKa = 3.29VPKK67 pKa = 10.81GCEE70 pKa = 4.39GPCKK74 pKa = 9.23VQSYY78 pKa = 7.95EE79 pKa = 3.85KK80 pKa = 10.56KK81 pKa = 10.39NDD83 pKa = 3.44VGHH86 pKa = 6.71SGTLLCVSDD95 pKa = 3.33ITRR98 pKa = 11.84GNGLTHH104 pKa = 6.89RR105 pKa = 11.84VGKK108 pKa = 9.82RR109 pKa = 11.84FCIKK113 pKa = 9.93SVYY116 pKa = 10.03IIGKK120 pKa = 8.43IWMDD124 pKa = 3.48EE125 pKa = 4.05NIKK128 pKa = 9.34TKK130 pKa = 10.62NHH132 pKa = 5.4TNNVLFWLVRR142 pKa = 11.84DD143 pKa = 4.01RR144 pKa = 11.84RR145 pKa = 11.84SGSTPYY151 pKa = 10.84GFQEE155 pKa = 4.07AFNMFEE161 pKa = 4.62NEE163 pKa = 3.81PSTATVKK170 pKa = 10.75QEE172 pKa = 3.83LRR174 pKa = 11.84DD175 pKa = 3.75RR176 pKa = 11.84LQVLHH181 pKa = 6.91RR182 pKa = 11.84FSATVTGGQYY192 pKa = 10.83ASKK195 pKa = 9.34EE196 pKa = 3.58QAIIKK201 pKa = 9.21RR202 pKa = 11.84FWKK205 pKa = 10.26LNHH208 pKa = 6.28HH209 pKa = 5.5VTYY212 pKa = 10.93NHH214 pKa = 6.12QEE216 pKa = 3.62QAKK219 pKa = 9.66YY220 pKa = 10.54EE221 pKa = 4.12NHH223 pKa = 6.33TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.77ATLKK246 pKa = 9.27IRR248 pKa = 11.84VYY250 pKa = 10.64FYY252 pKa = 11.38DD253 pKa = 3.47SVQNN257 pKa = 3.87

Molecular weight: 29.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1098

97

362

183.0

20.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.829 ± 0.547

2.186 ± 0.554

4.645 ± 0.5

5.191 ± 0.667

4.281 ± 0.512

4.827 ± 0.485

4.372 ± 0.689

5.92 ± 0.839

5.556 ± 0.725

7.195 ± 0.657

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.376

5.191 ± 0.635

5.556 ± 0.526

5.282 ± 0.675

6.557 ± 0.914

8.288 ± 1.325

6.557 ± 0.766

5.009 ± 1.02

1.457 ± 0.183

4.007 ± 0.5
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski