Candidatus Nitrosotalea sinensis

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Thaumarchaeota incertae sedis; Candidatus Nitrosotalea

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1881 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H1EHI7|A0A2H1EHI7_9ARCH Ribokinase-like domain-containing protein OS=Candidatus Nitrosotalea sinensis OX=1499975 GN=NSIN_30110 PE=4 SV=1
MM1 pKa = 7.33TNINAKK7 pKa = 8.84ATGPSGAPVTFSATATDD24 pKa = 4.14IVDD27 pKa = 3.5GTDD30 pKa = 4.4PVTCDD35 pKa = 3.29HH36 pKa = 6.84NSGDD40 pKa = 4.16TYY42 pKa = 11.19PLGSTTVSCSSTDD55 pKa = 3.29HH56 pKa = 7.08AGNTSHH62 pKa = 7.38ASFTITVAEE71 pKa = 4.44TTAPSITLPGTITQEE86 pKa = 3.69ATGPSGAPVTFSATATDD103 pKa = 4.14IVDD106 pKa = 3.5GTDD109 pKa = 3.49PVTCTPASGSTFALGSTTVNCSSTDD134 pKa = 3.09AHH136 pKa = 6.64GNTASGSFHH145 pKa = 6.29ITVQDD150 pKa = 3.22TTAPVLALPTPITATATSTAGAPVTFSATATDD182 pKa = 4.14IVDD185 pKa = 3.5GTDD188 pKa = 3.49PVTCTPASGSTFALGTATVTCTTSDD213 pKa = 3.29LHH215 pKa = 7.82SNTSTGSFTVTVNPGPVSTVKK236 pKa = 10.59ISPPTATTIVGSTPIQFTATSQDD259 pKa = 2.92QFGNIRR265 pKa = 11.84TGDD268 pKa = 3.52TLTWSVVSGGGSISSTGLYY287 pKa = 9.69TPGTTADD294 pKa = 3.66TATITATDD302 pKa = 4.16GSVSGTATITLNPGQVTSVTVSPSTVTLTSTQTQQFTATSQDD344 pKa = 2.92QFGNIRR350 pKa = 11.84TGDD353 pKa = 3.52TLTWSVVSGGGSISSTGLYY372 pKa = 9.7TPGTTAGTTVIAATDD387 pKa = 3.61NSISGSAIVTANNPNTIVQNQGGANLKK414 pKa = 10.22GDD416 pKa = 3.62NLQFLNLSNFNLSGDD431 pKa = 3.68NLKK434 pKa = 10.91GVTLQGSDD442 pKa = 3.46LQGANLSGANLAGANLQNVNLAGANLSGANLQGVNLSGANLQGANLKK489 pKa = 9.75GANLQGTNLQNDD501 pKa = 3.85NLLGADD507 pKa = 3.59LQGNNLQGANLSGANLQNANLSGANLQGANLQNANISGANLTGANVHH554 pKa = 6.27KK555 pKa = 10.81ANLQGTITSGITPSTLSGCLNNSVCQQ581 pKa = 3.61

Molecular weight: 57.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H1EH61|A0A2H1EH61_9ARCH Uncharacterized protein OS=Candidatus Nitrosotalea sinensis OX=1499975 GN=NSIN_20732 PE=4 SV=1
MM1 pKa = 7.67AAHH4 pKa = 7.52KK5 pKa = 7.68STPRR9 pKa = 11.84KK10 pKa = 9.0NRR12 pKa = 11.84LMKK15 pKa = 10.27KK16 pKa = 9.32IKK18 pKa = 9.65QNKK21 pKa = 8.43SVPAWIIVRR30 pKa = 11.84TKK32 pKa = 10.48RR33 pKa = 11.84RR34 pKa = 11.84VRR36 pKa = 11.84TNPKK40 pKa = 8.23KK41 pKa = 9.64RR42 pKa = 11.84QWRR45 pKa = 11.84RR46 pKa = 11.84TDD48 pKa = 3.12VKK50 pKa = 11.16VGG52 pKa = 3.24

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1881

0

1881

481166

23

1824

255.8

28.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.423 ± 0.06

0.961 ± 0.021

5.513 ± 0.045

5.781 ± 0.069

4.13 ± 0.04

6.737 ± 0.065

1.952 ± 0.021

8.552 ± 0.059

7.942 ± 0.077

8.615 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.034

4.778 ± 0.058

3.948 ± 0.044

3.445 ± 0.042

3.853 ± 0.047

7.498 ± 0.066

6.035 ± 0.073

6.975 ± 0.054

0.907 ± 0.019

3.249 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski