Meyerozyma sp. JA9

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Debaryomycetaceae; Meyerozyma; unclassified Meyerozyma

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5012 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A421JAI0|A0A421JAI0_9ASCO Nuclear import protein MOG1 OS=Meyerozyma sp. JA9 OX=2028340 GN=JA9_001901 PE=3 SV=1
MM1 pKa = 7.76KK2 pKa = 9.26ITTIFHH8 pKa = 5.87TVLLASAAVNAQDD21 pKa = 3.59APKK24 pKa = 10.51KK25 pKa = 10.44RR26 pKa = 11.84GFLSDD31 pKa = 3.89LLGGSGSQAAAAPAAAAAAPAAAAPAAASSVAAPAAAPAASSAATTSTTAAAAAAAAPVDD91 pKa = 4.14ATSSAAGVLDD101 pKa = 4.85LFGLDD106 pKa = 3.52SSSSNSTSSSSSSSSSSSSSSSSTSSTKK134 pKa = 10.54AAGFRR139 pKa = 11.84ALAAQTTQSSSSAAASTEE157 pKa = 3.58AGLFDD162 pKa = 5.09FLNADD167 pKa = 3.49SSSALSSASSSASSSALSSSSTEE190 pKa = 3.51AGLFDD195 pKa = 5.09FLNADD200 pKa = 3.62SSSASSSIASSSSLSSSLSSSASASASSKK229 pKa = 9.53GHH231 pKa = 6.01SGGLFNLFAAAGSGSASSASASKK254 pKa = 10.53SASASSASASSSGSPSASTTGGGFLDD280 pKa = 4.43DD281 pKa = 4.75LFGGSSSSNSSASSGSASSSASADD305 pKa = 3.72DD306 pKa = 4.48EE307 pKa = 4.5EE308 pKa = 6.71CDD310 pKa = 5.55DD311 pKa = 4.49EE312 pKa = 4.66TDD314 pKa = 3.36SAAPSGIVPPLSSMSLPPLTGSAPTGSLPPLTGMSSQPSGGSRR357 pKa = 11.84TLAASSSAPASASAHH372 pKa = 5.84ASSVSDD378 pKa = 6.2DD379 pKa = 3.8EE380 pKa = 6.84DD381 pKa = 3.88EE382 pKa = 4.68DD383 pKa = 5.24CEE385 pKa = 4.58TEE387 pKa = 4.33STPSLPPLSGTGGSLPPLSGTGGSLPPLSGTGGVLPTLASTTKK430 pKa = 10.1NGSVTSATVPRR441 pKa = 11.84STASEE446 pKa = 4.68DD447 pKa = 4.05DD448 pKa = 5.85DD449 pKa = 4.76EE450 pKa = 7.13DD451 pKa = 5.99CDD453 pKa = 4.91TEE455 pKa = 4.38TTTPGGVISEE465 pKa = 4.53TKK467 pKa = 8.01TTITTVKK474 pKa = 6.8TTNGAGSAVTYY485 pKa = 7.05TTTKK489 pKa = 9.5ATTKK493 pKa = 10.41AVPTQSSDD501 pKa = 4.68DD502 pKa = 3.98EE503 pKa = 4.7DD504 pKa = 4.84CEE506 pKa = 4.11EE507 pKa = 5.29YY508 pKa = 9.75YY509 pKa = 10.56TEE511 pKa = 4.14TQTFYY516 pKa = 8.66TTYY519 pKa = 7.55TTVVNGATVTKK530 pKa = 10.48VVEE533 pKa = 4.35CEE535 pKa = 4.07SCNTGSGNAGGSGSSGNTGSSGNSGSSGSSPGSGVDD571 pKa = 3.48VDD573 pKa = 5.07CEE575 pKa = 4.19DD576 pKa = 5.14DD577 pKa = 3.79EE578 pKa = 5.32GSSGSSGSSGSSGSSGSSGSSGSSGSSGSSGSSGSSPGYY617 pKa = 10.05LIPSDD622 pKa = 4.08DD623 pKa = 4.06QCDD626 pKa = 3.92EE627 pKa = 4.4DD628 pKa = 6.25CVVCDD633 pKa = 5.87DD634 pKa = 6.14DD635 pKa = 7.78DD636 pKa = 7.6DD637 pKa = 6.13DD638 pKa = 6.44FDD640 pKa = 4.14WFGNDD645 pKa = 4.3TDD647 pKa = 5.56DD648 pKa = 5.23EE649 pKa = 5.81CDD651 pKa = 4.47DD652 pKa = 4.7DD653 pKa = 6.69CEE655 pKa = 4.32EE656 pKa = 4.75CDD658 pKa = 4.57AGTGTKK664 pKa = 10.06GGSGSGSGGSSGSGSGSGSGSGSGSGSGSGSGSGSGSGGSGAEE707 pKa = 4.1TYY709 pKa = 11.12VSIIRR714 pKa = 11.84TTAAGEE720 pKa = 4.36STWYY724 pKa = 8.1STSVSTSTHH733 pKa = 6.05SPDD736 pKa = 5.14DD737 pKa = 3.66EE738 pKa = 6.59CDD740 pKa = 3.81DD741 pKa = 4.41EE742 pKa = 5.67EE743 pKa = 5.13STTTTKK749 pKa = 10.87NATSTTSGYY758 pKa = 9.29TNSTSKK764 pKa = 8.72QTGGFLADD772 pKa = 3.76LFGRR776 pKa = 11.84DD777 pKa = 3.73VQVSSSAWVNASTTSTPSVEE797 pKa = 4.15PQLSGSRR804 pKa = 11.84QTSFSTALVALLVSLVAFTLII825 pKa = 4.19

Molecular weight: 78.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A421JD04|A0A421JD04_9ASCO 37S ribosomal protein SWS2 mitochondrial OS=Meyerozyma sp. JA9 OX=2028340 GN=JA9_001198 PE=3 SV=1
MM1 pKa = 7.61PVQRR5 pKa = 11.84LRR7 pKa = 11.84DD8 pKa = 3.51LAAQKK13 pKa = 10.31CVQNVALLHH22 pKa = 6.71DD23 pKa = 4.25VGDD26 pKa = 4.14TPFHH30 pKa = 6.5ILRR33 pKa = 11.84PILCRR38 pKa = 11.84MSARR42 pKa = 11.84QLDD45 pKa = 4.27SIEE48 pKa = 4.55SKK50 pKa = 10.24SCQIVPYY57 pKa = 10.5SDD59 pKa = 3.89QLWPNLIARR68 pKa = 11.84DD69 pKa = 4.04FPNRR73 pKa = 11.84PLASRR78 pKa = 11.84PLIDD82 pKa = 3.3TPMPHH87 pKa = 6.81KK88 pKa = 10.62ALYY91 pKa = 9.62FKK93 pKa = 11.03YY94 pKa = 9.96LAEE97 pKa = 4.55RR98 pKa = 11.84EE99 pKa = 4.02ALQRR103 pKa = 11.84DD104 pKa = 3.61SAQRR108 pKa = 11.84LRR110 pKa = 11.84SMTQRR115 pKa = 11.84LRR117 pKa = 11.84QEE119 pKa = 3.8KK120 pKa = 9.71SKK122 pKa = 11.28NAITPVKK129 pKa = 10.16HH130 pKa = 6.52LLRR133 pKa = 11.84DD134 pKa = 3.37PTIRR138 pKa = 11.84RR139 pKa = 11.84RR140 pKa = 11.84PVSTSWSKK148 pKa = 11.14SSQSLSSLSIVQRR161 pKa = 11.84ARR163 pKa = 11.84RR164 pKa = 11.84EE165 pKa = 3.75TRR167 pKa = 11.84EE168 pKa = 3.75RR169 pKa = 11.84SRR171 pKa = 11.84LFQKK175 pKa = 10.37NVPKK179 pKa = 10.87DD180 pKa = 3.44PFASNNNNNNNNNNNNTCPCSTCTCQDD207 pKa = 3.16HH208 pKa = 6.3TNLRR212 pKa = 3.9

Molecular weight: 24.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5012

0

5012

2648595

59

4900

528.5

59.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.738 ± 0.029

1.153 ± 0.01

5.869 ± 0.026

6.469 ± 0.033

4.329 ± 0.024

5.624 ± 0.033

2.326 ± 0.014

5.744 ± 0.023

6.399 ± 0.033

9.226 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.045 ± 0.012

4.818 ± 0.02

4.835 ± 0.029

3.928 ± 0.024

4.778 ± 0.019

9.104 ± 0.038

5.665 ± 0.043

6.448 ± 0.025

1.098 ± 0.012

3.401 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski