Amino acid dipepetide frequency for Brucella phage EF4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.514AlaAla: 9.514 ± 1.314
0.898AlaCys: 0.898 ± 0.288
5.744AlaAsp: 5.744 ± 0.672
5.924AlaGlu: 5.924 ± 0.825
2.872AlaPhe: 2.872 ± 0.559
6.821AlaGly: 6.821 ± 0.787
1.436AlaHis: 1.436 ± 0.342
5.116AlaIle: 5.116 ± 0.755
5.475AlaLys: 5.475 ± 0.923
8.975AlaLeu: 8.975 ± 0.915
1.885AlaMet: 1.885 ± 0.385
4.129AlaAsn: 4.129 ± 0.755
3.77AlaPro: 3.77 ± 0.911
5.565AlaGln: 5.565 ± 0.937
5.026AlaArg: 5.026 ± 0.706
6.283AlaSer: 6.283 ± 0.87
4.218AlaThr: 4.218 ± 0.637
5.565AlaVal: 5.565 ± 0.732
1.705AlaTrp: 1.705 ± 0.39
1.705AlaTyr: 1.705 ± 0.43
0.0AlaXaa: 0.0 ± 0.0
Cys
0.718CysAla: 0.718 ± 0.25
0.18CysCys: 0.18 ± 0.127
0.449CysAsp: 0.449 ± 0.166
0.898CysGlu: 0.898 ± 0.255
0.359CysPhe: 0.359 ± 0.171
1.257CysGly: 1.257 ± 0.323
0.18CysHis: 0.18 ± 0.123
0.987CysIle: 0.987 ± 0.282
0.449CysLys: 0.449 ± 0.21
0.898CysLeu: 0.898 ± 0.325
0.808CysMet: 0.808 ± 0.26
0.359CysAsn: 0.359 ± 0.148
0.898CysPro: 0.898 ± 0.306
0.539CysGln: 0.539 ± 0.256
1.257CysArg: 1.257 ± 0.301
0.628CysSer: 0.628 ± 0.261
0.449CysThr: 0.449 ± 0.229
0.718CysVal: 0.718 ± 0.269
0.09CysTrp: 0.09 ± 0.089
0.269CysTyr: 0.269 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
4.667AspAla: 4.667 ± 0.614
0.628AspCys: 0.628 ± 0.229
3.77AspAsp: 3.77 ± 0.614
4.308AspGlu: 4.308 ± 0.741
2.423AspPhe: 2.423 ± 0.402
4.488AspGly: 4.488 ± 0.458
1.795AspHis: 1.795 ± 0.391
4.577AspIle: 4.577 ± 0.672
2.603AspLys: 2.603 ± 0.535
4.577AspLeu: 4.577 ± 0.651
1.885AspMet: 1.885 ± 0.344
2.244AspAsn: 2.244 ± 0.396
2.962AspPro: 2.962 ± 0.472
2.244AspGln: 2.244 ± 0.485
3.68AspArg: 3.68 ± 0.51
2.693AspSer: 2.693 ± 0.531
2.513AspThr: 2.513 ± 0.437
3.141AspVal: 3.141 ± 0.554
0.898AspTrp: 0.898 ± 0.355
2.693AspTyr: 2.693 ± 0.515
0.0AspXaa: 0.0 ± 0.0
Glu
6.911GluAla: 6.911 ± 0.914
0.539GluCys: 0.539 ± 0.221
3.5GluAsp: 3.5 ± 0.559
3.949GluGlu: 3.949 ± 0.711
2.244GluPhe: 2.244 ± 0.523
3.949GluGly: 3.949 ± 0.602
1.616GluHis: 1.616 ± 0.422
3.859GluIle: 3.859 ± 0.757
3.859GluLys: 3.859 ± 0.693
7.09GluLeu: 7.09 ± 0.945
2.334GluMet: 2.334 ± 0.413
2.334GluAsn: 2.334 ± 0.448
2.244GluPro: 2.244 ± 0.598
2.962GluGln: 2.962 ± 0.516
4.847GluArg: 4.847 ± 0.594
3.5GluSer: 3.5 ± 0.556
3.052GluThr: 3.052 ± 0.421
6.013GluVal: 6.013 ± 0.957
1.167GluTrp: 1.167 ± 0.42
2.154GluTyr: 2.154 ± 0.401
0.0GluXaa: 0.0 ± 0.0
Phe
3.141PheAla: 3.141 ± 0.46
1.167PheCys: 1.167 ± 0.262
2.154PheAsp: 2.154 ± 0.409
1.526PheGlu: 1.526 ± 0.337
1.526PhePhe: 1.526 ± 0.475
2.693PheGly: 2.693 ± 0.575
0.808PheHis: 0.808 ± 0.252
1.346PheIle: 1.346 ± 0.322
1.616PheLys: 1.616 ± 0.343
1.885PheLeu: 1.885 ± 0.437
1.616PheMet: 1.616 ± 0.358
1.346PheAsn: 1.346 ± 0.361
1.526PhePro: 1.526 ± 0.31
1.257PheGln: 1.257 ± 0.35
0.987PheArg: 0.987 ± 0.318
2.334PheSer: 2.334 ± 0.472
2.244PheThr: 2.244 ± 0.433
1.795PheVal: 1.795 ± 0.354
0.449PheTrp: 0.449 ± 0.195
0.539PheTyr: 0.539 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
6.642GlyAla: 6.642 ± 0.936
0.987GlyCys: 0.987 ± 0.328
3.859GlyAsp: 3.859 ± 0.606
3.68GlyGlu: 3.68 ± 0.653
3.321GlyPhe: 3.321 ± 0.613
8.616GlyGly: 8.616 ± 1.477
0.808GlyHis: 0.808 ± 0.261
3.052GlyIle: 3.052 ± 0.496
5.295GlyLys: 5.295 ± 0.741
7.001GlyLeu: 7.001 ± 0.7
2.334GlyMet: 2.334 ± 0.518
4.129GlyAsn: 4.129 ± 0.699
2.782GlyPro: 2.782 ± 0.404
1.975GlyGln: 1.975 ± 0.554
4.757GlyArg: 4.757 ± 0.872
5.475GlySer: 5.475 ± 0.874
4.039GlyThr: 4.039 ± 0.635
5.206GlyVal: 5.206 ± 0.679
1.436GlyTrp: 1.436 ± 0.492
2.782GlyTyr: 2.782 ± 0.654
0.0GlyXaa: 0.0 ± 0.0
His
1.257HisAla: 1.257 ± 0.365
0.269HisCys: 0.269 ± 0.154
0.898HisAsp: 0.898 ± 0.263
0.898HisGlu: 0.898 ± 0.21
0.808HisPhe: 0.808 ± 0.268
1.705HisGly: 1.705 ± 0.407
0.449HisHis: 0.449 ± 0.168
1.346HisIle: 1.346 ± 0.327
1.077HisLys: 1.077 ± 0.314
1.526HisLeu: 1.526 ± 0.366
0.269HisMet: 0.269 ± 0.152
0.898HisAsn: 0.898 ± 0.331
1.077HisPro: 1.077 ± 0.275
0.628HisGln: 0.628 ± 0.228
0.898HisArg: 0.898 ± 0.257
0.987HisSer: 0.987 ± 0.283
0.628HisThr: 0.628 ± 0.221
1.346HisVal: 1.346 ± 0.324
0.269HisTrp: 0.269 ± 0.155
0.539HisTyr: 0.539 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
5.295IleAla: 5.295 ± 0.683
0.898IleCys: 0.898 ± 0.266
4.667IleAsp: 4.667 ± 0.863
5.026IleGlu: 5.026 ± 0.718
1.795IlePhe: 1.795 ± 0.369
4.308IleGly: 4.308 ± 0.705
0.539IleHis: 0.539 ± 0.237
3.5IleIle: 3.5 ± 0.485
3.231IleLys: 3.231 ± 0.659
4.847IleLeu: 4.847 ± 0.558
0.987IleMet: 0.987 ± 0.286
2.513IleAsn: 2.513 ± 0.453
2.872IlePro: 2.872 ± 0.496
1.795IleGln: 1.795 ± 0.437
1.975IleArg: 1.975 ± 0.366
4.218IleSer: 4.218 ± 0.569
2.603IleThr: 2.603 ± 0.437
2.962IleVal: 2.962 ± 0.732
0.808IleTrp: 0.808 ± 0.303
1.616IleTyr: 1.616 ± 0.382
0.0IleXaa: 0.0 ± 0.0
Lys
5.385LysAla: 5.385 ± 0.928
0.808LysCys: 0.808 ± 0.285
3.5LysAsp: 3.5 ± 0.725
5.026LysGlu: 5.026 ± 0.783
1.436LysPhe: 1.436 ± 0.365
3.59LysGly: 3.59 ± 0.668
1.346LysHis: 1.346 ± 0.408
3.859LysIle: 3.859 ± 0.713
3.59LysLys: 3.59 ± 0.63
3.5LysLeu: 3.5 ± 0.556
1.795LysMet: 1.795 ± 0.425
1.705LysAsn: 1.705 ± 0.426
2.962LysPro: 2.962 ± 0.546
1.257LysGln: 1.257 ± 0.508
3.68LysArg: 3.68 ± 0.546
4.129LysSer: 4.129 ± 0.643
2.603LysThr: 2.603 ± 0.499
3.052LysVal: 3.052 ± 0.538
0.898LysTrp: 0.898 ± 0.239
1.975LysTyr: 1.975 ± 0.441
0.0LysXaa: 0.0 ± 0.0
Leu
7.27LeuAla: 7.27 ± 0.941
1.077LeuCys: 1.077 ± 0.33
3.859LeuAsp: 3.859 ± 0.516
4.936LeuGlu: 4.936 ± 0.831
1.795LeuPhe: 1.795 ± 0.455
5.475LeuGly: 5.475 ± 0.659
0.718LeuHis: 0.718 ± 0.268
4.936LeuIle: 4.936 ± 0.682
4.757LeuLys: 4.757 ± 0.819
3.949LeuLeu: 3.949 ± 0.618
1.795LeuMet: 1.795 ± 0.343
4.218LeuAsn: 4.218 ± 0.568
4.488LeuPro: 4.488 ± 0.948
4.039LeuGln: 4.039 ± 0.811
4.488LeuArg: 4.488 ± 0.783
6.552LeuSer: 6.552 ± 0.697
4.936LeuThr: 4.936 ± 0.639
4.667LeuVal: 4.667 ± 0.741
1.257LeuTrp: 1.257 ± 0.376
1.705LeuTyr: 1.705 ± 0.335
0.0LeuXaa: 0.0 ± 0.0
Met
2.872MetAla: 2.872 ± 0.545
0.269MetCys: 0.269 ± 0.139
1.346MetAsp: 1.346 ± 0.294
1.167MetGlu: 1.167 ± 0.387
0.628MetPhe: 0.628 ± 0.227
2.782MetGly: 2.782 ± 0.434
0.539MetHis: 0.539 ± 0.226
0.987MetIle: 0.987 ± 0.291
1.885MetLys: 1.885 ± 0.388
2.513MetLeu: 2.513 ± 0.393
1.167MetMet: 1.167 ± 0.371
0.808MetAsn: 0.808 ± 0.247
1.436MetPro: 1.436 ± 0.278
1.616MetGln: 1.616 ± 0.404
1.885MetArg: 1.885 ± 0.345
2.154MetSer: 2.154 ± 0.329
2.154MetThr: 2.154 ± 0.43
1.705MetVal: 1.705 ± 0.374
0.449MetTrp: 0.449 ± 0.19
1.077MetTyr: 1.077 ± 0.3
0.0MetXaa: 0.0 ± 0.0
Asn
4.488AsnAla: 4.488 ± 0.674
0.18AsnCys: 0.18 ± 0.123
2.693AsnAsp: 2.693 ± 0.456
2.423AsnGlu: 2.423 ± 0.378
1.077AsnPhe: 1.077 ± 0.343
3.5AsnGly: 3.5 ± 0.621
0.628AsnHis: 0.628 ± 0.248
2.064AsnIle: 2.064 ± 0.445
1.795AsnLys: 1.795 ± 0.394
3.321AsnLeu: 3.321 ± 0.531
1.436AsnMet: 1.436 ± 0.314
2.244AsnAsn: 2.244 ± 0.666
3.411AsnPro: 3.411 ± 0.72
1.885AsnGln: 1.885 ± 0.541
2.782AsnArg: 2.782 ± 0.417
1.975AsnSer: 1.975 ± 0.455
2.693AsnThr: 2.693 ± 0.537
2.154AsnVal: 2.154 ± 0.449
1.346AsnTrp: 1.346 ± 0.33
1.436AsnTyr: 1.436 ± 0.361
0.0AsnXaa: 0.0 ± 0.0
Pro
3.859ProAla: 3.859 ± 0.703
0.628ProCys: 0.628 ± 0.226
3.68ProAsp: 3.68 ± 0.535
4.936ProGlu: 4.936 ± 0.701
2.782ProPhe: 2.782 ± 0.4
3.411ProGly: 3.411 ± 0.538
0.987ProHis: 0.987 ± 0.26
2.064ProIle: 2.064 ± 0.462
2.154ProLys: 2.154 ± 0.488
2.872ProLeu: 2.872 ± 0.538
1.167ProMet: 1.167 ± 0.293
1.975ProAsn: 1.975 ± 0.382
2.513ProPro: 2.513 ± 0.577
2.244ProGln: 2.244 ± 0.586
1.975ProArg: 1.975 ± 0.411
3.68ProSer: 3.68 ± 0.644
2.513ProThr: 2.513 ± 0.512
3.68ProVal: 3.68 ± 0.625
0.449ProTrp: 0.449 ± 0.25
2.064ProTyr: 2.064 ± 0.398
0.0ProXaa: 0.0 ± 0.0
Gln
5.206GlnAla: 5.206 ± 0.781
0.449GlnCys: 0.449 ± 0.199
2.603GlnAsp: 2.603 ± 0.439
3.141GlnGlu: 3.141 ± 0.638
1.167GlnPhe: 1.167 ± 0.364
3.141GlnGly: 3.141 ± 0.512
0.628GlnHis: 0.628 ± 0.263
2.244GlnIle: 2.244 ± 0.464
1.795GlnLys: 1.795 ± 0.369
3.59GlnLeu: 3.59 ± 0.586
1.436GlnMet: 1.436 ± 0.334
1.795GlnAsn: 1.795 ± 0.354
1.885GlnPro: 1.885 ± 0.546
2.603GlnGln: 2.603 ± 0.861
2.154GlnArg: 2.154 ± 0.545
3.052GlnSer: 3.052 ± 0.627
2.423GlnThr: 2.423 ± 0.404
2.244GlnVal: 2.244 ± 0.551
0.449GlnTrp: 0.449 ± 0.204
1.077GlnTyr: 1.077 ± 0.307
0.0GlnXaa: 0.0 ± 0.0
Arg
4.308ArgAla: 4.308 ± 0.385
1.257ArgCys: 1.257 ± 0.305
3.231ArgAsp: 3.231 ± 0.427
5.385ArgGlu: 5.385 ± 0.69
1.167ArgPhe: 1.167 ± 0.309
3.231ArgGly: 3.231 ± 0.513
0.808ArgHis: 0.808 ± 0.246
3.68ArgIle: 3.68 ± 0.613
3.949ArgLys: 3.949 ± 0.557
4.308ArgLeu: 4.308 ± 0.581
1.795ArgMet: 1.795 ± 0.385
2.962ArgAsn: 2.962 ± 0.466
2.334ArgPro: 2.334 ± 0.489
2.782ArgGln: 2.782 ± 0.572
3.5ArgArg: 3.5 ± 0.567
3.052ArgSer: 3.052 ± 0.542
2.064ArgThr: 2.064 ± 0.397
4.308ArgVal: 4.308 ± 0.503
1.167ArgTrp: 1.167 ± 0.237
2.244ArgTyr: 2.244 ± 0.432
0.0ArgXaa: 0.0 ± 0.0
Ser
6.103SerAla: 6.103 ± 0.746
0.359SerCys: 0.359 ± 0.17
3.052SerAsp: 3.052 ± 0.515
4.129SerGlu: 4.129 ± 0.665
1.795SerPhe: 1.795 ± 0.474
5.834SerGly: 5.834 ± 0.685
1.436SerHis: 1.436 ± 0.333
3.231SerIle: 3.231 ± 0.597
3.77SerLys: 3.77 ± 0.618
5.654SerLeu: 5.654 ± 0.761
1.975SerMet: 1.975 ± 0.383
3.411SerAsn: 3.411 ± 0.54
3.77SerPro: 3.77 ± 0.853
3.231SerGln: 3.231 ± 0.61
3.949SerArg: 3.949 ± 0.745
5.385SerSer: 5.385 ± 0.723
2.962SerThr: 2.962 ± 0.455
4.129SerVal: 4.129 ± 0.526
0.628SerTrp: 0.628 ± 0.261
2.603SerTyr: 2.603 ± 0.483
0.0SerXaa: 0.0 ± 0.0
Thr
4.577ThrAla: 4.577 ± 0.64
0.09ThrCys: 0.09 ± 0.089
2.154ThrAsp: 2.154 ± 0.443
2.693ThrGlu: 2.693 ± 0.532
1.346ThrPhe: 1.346 ± 0.379
5.565ThrGly: 5.565 ± 0.738
1.077ThrHis: 1.077 ± 0.323
3.052ThrIle: 3.052 ± 0.503
3.321ThrLys: 3.321 ± 0.614
4.129ThrLeu: 4.129 ± 0.591
1.257ThrMet: 1.257 ± 0.406
1.705ThrAsn: 1.705 ± 0.347
4.039ThrPro: 4.039 ± 0.73
1.705ThrGln: 1.705 ± 0.379
2.064ThrArg: 2.064 ± 0.49
3.321ThrSer: 3.321 ± 0.562
2.872ThrThr: 2.872 ± 0.526
2.872ThrVal: 2.872 ± 0.42
1.077ThrTrp: 1.077 ± 0.362
1.885ThrTyr: 1.885 ± 0.389
0.0ThrXaa: 0.0 ± 0.0
Val
4.936ValAla: 4.936 ± 0.713
0.898ValCys: 0.898 ± 0.301
4.847ValAsp: 4.847 ± 0.555
4.757ValGlu: 4.757 ± 0.811
1.705ValPhe: 1.705 ± 0.434
4.488ValGly: 4.488 ± 0.767
0.987ValHis: 0.987 ± 0.371
4.129ValIle: 4.129 ± 0.605
3.321ValLys: 3.321 ± 0.562
3.59ValLeu: 3.59 ± 0.459
1.975ValMet: 1.975 ± 0.391
2.513ValAsn: 2.513 ± 0.445
2.513ValPro: 2.513 ± 0.457
1.975ValGln: 1.975 ± 0.414
4.039ValArg: 4.039 ± 0.644
4.847ValSer: 4.847 ± 0.622
3.5ValThr: 3.5 ± 0.564
2.872ValVal: 2.872 ± 0.474
0.718ValTrp: 0.718 ± 0.285
2.513ValTyr: 2.513 ± 0.491
0.0ValXaa: 0.0 ± 0.0
Trp
1.705TrpAla: 1.705 ± 0.34
0.18TrpCys: 0.18 ± 0.133
1.167TrpAsp: 1.167 ± 0.363
1.167TrpGlu: 1.167 ± 0.374
0.628TrpPhe: 0.628 ± 0.206
0.808TrpGly: 0.808 ± 0.313
0.449TrpHis: 0.449 ± 0.164
0.808TrpIle: 0.808 ± 0.269
0.808TrpLys: 0.808 ± 0.26
0.987TrpLeu: 0.987 ± 0.297
0.718TrpMet: 0.718 ± 0.252
0.539TrpAsn: 0.539 ± 0.183
0.808TrpPro: 0.808 ± 0.344
0.987TrpGln: 0.987 ± 0.247
0.898TrpArg: 0.898 ± 0.32
1.346TrpSer: 1.346 ± 0.316
0.718TrpThr: 0.718 ± 0.228
0.898TrpVal: 0.898 ± 0.284
0.359TrpTrp: 0.359 ± 0.177
0.269TrpTyr: 0.269 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.77TyrAla: 3.77 ± 0.61
0.628TyrCys: 0.628 ± 0.241
1.616TyrAsp: 1.616 ± 0.298
2.154TyrGlu: 2.154 ± 0.496
1.077TyrPhe: 1.077 ± 0.316
2.513TyrGly: 2.513 ± 0.4
0.539TyrHis: 0.539 ± 0.226
1.616TyrIle: 1.616 ± 0.35
1.346TyrLys: 1.346 ± 0.413
1.705TyrLeu: 1.705 ± 0.379
0.718TyrMet: 0.718 ± 0.253
1.705TyrAsn: 1.705 ± 0.452
1.346TyrPro: 1.346 ± 0.283
1.795TyrGln: 1.795 ± 0.372
2.782TyrArg: 2.782 ± 0.376
1.795TyrSer: 1.795 ± 0.374
1.616TyrThr: 1.616 ± 0.424
1.795TyrVal: 1.795 ± 0.345
0.628TyrTrp: 0.628 ± 0.241
0.808TyrTyr: 0.808 ± 0.225
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (11143 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski