Pochonia chlamydosporia 123

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Clavicipitaceae; Pochonia; Pochonia chlamydosporia

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11960 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7KB42|A0A4Q7KB42_METCM Putative aldehyde dehydrogenase OS=Pochonia chlamydosporia 123 OX=1052797 GN=I1G_00000915 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.43ANSCSFIALRR12 pKa = 11.84ILLLAITSTAQPPPTIQCPSLPPAQYY38 pKa = 10.9ASRR41 pKa = 11.84IQCEE45 pKa = 4.0NHH47 pKa = 6.61DD48 pKa = 3.92GSVVEE53 pKa = 4.44VNGPRR58 pKa = 11.84PFNCKK63 pKa = 9.96LSWVAIRR70 pKa = 11.84PLTTKK75 pKa = 7.77TTTHH79 pKa = 5.92EE80 pKa = 4.01QQEE83 pKa = 4.45PLNEE87 pKa = 4.06EE88 pKa = 4.41HH89 pKa = 6.49GQCALWLFQFSDD101 pKa = 4.15DD102 pKa = 4.14GLVQQSCCGEE112 pKa = 4.02AGEE115 pKa = 4.61GLVIRR120 pKa = 11.84ASRR123 pKa = 11.84VQEE126 pKa = 3.87QHH128 pKa = 6.7DD129 pKa = 4.2QDD131 pKa = 3.44WDD133 pKa = 3.73PTQEE137 pKa = 3.29WMEE140 pKa = 3.75FHH142 pKa = 6.88EE143 pKa = 5.18GVEE146 pKa = 4.34EE147 pKa = 4.15PVQHH151 pKa = 6.39LQMDD155 pKa = 4.55DD156 pKa = 3.84PPVGGDD162 pKa = 3.25GAEE165 pKa = 3.89QHH167 pKa = 7.19DD168 pKa = 4.57DD169 pKa = 4.12DD170 pKa = 7.17GGDD173 pKa = 3.37EE174 pKa = 5.08HH175 pKa = 8.99GDD177 pKa = 3.49ADD179 pKa = 3.82EE180 pKa = 6.03DD181 pKa = 4.56GYY183 pKa = 11.5EE184 pKa = 4.95DD185 pKa = 4.85EE186 pKa = 5.9YY187 pKa = 11.48EE188 pKa = 4.84DD189 pKa = 5.9DD190 pKa = 5.4YY191 pKa = 11.96EE192 pKa = 6.59DD193 pKa = 3.91EE194 pKa = 5.69DD195 pKa = 5.34GDD197 pKa = 4.07IEE199 pKa = 5.1FGTGQQHH206 pKa = 5.96IFEE209 pKa = 4.53VDD211 pKa = 2.72

Molecular weight: 23.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7JHT9|A0A4Q7JHT9_METCM Uncharacterized protein OS=Pochonia chlamydosporia 123 OX=1052797 GN=I1G_00007633 PE=4 SV=1
MM1 pKa = 7.5PFGRR5 pKa = 11.84RR6 pKa = 11.84THH8 pKa = 5.94HH9 pKa = 6.3HH10 pKa = 6.12HH11 pKa = 5.95TTTTTRR17 pKa = 11.84PRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84GLFSRR26 pKa = 11.84RR27 pKa = 11.84PRR29 pKa = 11.84HH30 pKa = 4.64VHH32 pKa = 4.22HH33 pKa = 6.69QKK35 pKa = 10.52RR36 pKa = 11.84KK37 pKa = 7.71PTMKK41 pKa = 10.46DD42 pKa = 2.98KK43 pKa = 11.5VSGALMKK50 pKa = 10.31IRR52 pKa = 11.84GSLTRR57 pKa = 11.84RR58 pKa = 11.84PGLKK62 pKa = 10.1AAGTRR67 pKa = 11.84RR68 pKa = 11.84MHH70 pKa = 5.56GTDD73 pKa = 2.86GRR75 pKa = 11.84GHH77 pKa = 6.78HH78 pKa = 5.81KK79 pKa = 9.87HH80 pKa = 5.79AHH82 pKa = 5.35IFF84 pKa = 3.53

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11960

0

11960

5937693

62

9677

496.5

54.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.623 ± 0.019

1.282 ± 0.01

5.826 ± 0.018

5.911 ± 0.024

3.735 ± 0.014

7.002 ± 0.022

2.388 ± 0.008

4.949 ± 0.014

4.955 ± 0.02

8.846 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.275 ± 0.008

3.785 ± 0.012

5.788 ± 0.023

4.039 ± 0.017

5.885 ± 0.019

8.043 ± 0.026

5.979 ± 0.015

6.206 ± 0.017

1.517 ± 0.008

2.729 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski