Lactobacillus phage JNU_P10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3BFB0|A0A6M3BFB0_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P10 OX=2686380 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84KK3 pKa = 9.39YY4 pKa = 10.01EE5 pKa = 4.16CQDD8 pKa = 3.26MFSHH12 pKa = 6.95KK13 pKa = 10.64VIATFDD19 pKa = 3.33IYY21 pKa = 11.58DD22 pKa = 3.88EE23 pKa = 4.57ADD25 pKa = 2.94KK26 pKa = 11.28FMDD29 pKa = 3.39EE30 pKa = 4.85AYY32 pKa = 10.19DD33 pKa = 3.86YY34 pKa = 11.14SDD36 pKa = 3.06WWTVPAMTIVEE47 pKa = 4.35VTDD50 pKa = 3.51NADD53 pKa = 2.89

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3BFA3|A0A6M3BFA3_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P10 OX=2686380 PE=4 SV=1
MM1 pKa = 7.1LQYY4 pKa = 11.16GRR6 pKa = 11.84GLTGKK11 pKa = 9.8EE12 pKa = 3.74EE13 pKa = 4.16NKK15 pKa = 10.12MNDD18 pKa = 2.47RR19 pKa = 11.84HH20 pKa = 5.61RR21 pKa = 11.84AVMRR25 pKa = 11.84ARR27 pKa = 11.84IRR29 pKa = 11.84YY30 pKa = 6.94EE31 pKa = 3.63RR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 10.2HH35 pKa = 5.2EE36 pKa = 3.9RR37 pKa = 11.84TMDD40 pKa = 3.48EE41 pKa = 4.09FAKK44 pKa = 10.75ALYY47 pKa = 9.53PVFKK51 pKa = 10.43AAAATIEE58 pKa = 3.85QWLAAFQFRR67 pKa = 4.28

Molecular weight: 8.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12805

36

1029

188.3

21.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.419 ± 0.453

0.562 ± 0.116

6.849 ± 0.361

5.67 ± 0.411

3.889 ± 0.206

6.209 ± 0.447

1.765 ± 0.214

6.052 ± 0.24

7.114 ± 0.372

7.942 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.765 ± 0.163

5.014 ± 0.263

3.639 ± 0.271

4.483 ± 0.191

4.178 ± 0.408

7.286 ± 0.376

6.497 ± 0.278

6.318 ± 0.323

1.484 ± 0.196

3.866 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski