Amino acid dipepetide frequency for Streptomyces phage Joe

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.858AlaAla: 12.858 ± 1.166
0.466AlaCys: 0.466 ± 0.185
6.929AlaAsp: 6.929 ± 0.66
7.728AlaGlu: 7.728 ± 0.842
3.264AlaPhe: 3.264 ± 0.507
7.995AlaGly: 7.995 ± 0.787
1.399AlaHis: 1.399 ± 0.321
4.131AlaIle: 4.131 ± 0.548
4.53AlaLys: 4.53 ± 0.639
10.593AlaLeu: 10.593 ± 1.172
2.798AlaMet: 2.798 ± 0.336
3.464AlaAsn: 3.464 ± 0.446
4.997AlaPro: 4.997 ± 0.583
3.797AlaGln: 3.797 ± 0.517
6.396AlaArg: 6.396 ± 0.714
5.863AlaSer: 5.863 ± 0.633
6.063AlaThr: 6.063 ± 0.877
8.061AlaVal: 8.061 ± 0.822
2.199AlaTrp: 2.199 ± 0.362
3.398AlaTyr: 3.398 ± 0.52
0.0AlaXaa: 0.0 ± 0.0
Cys
0.6CysAla: 0.6 ± 0.284
0.333CysCys: 0.333 ± 0.147
0.733CysAsp: 0.733 ± 0.228
0.466CysGlu: 0.466 ± 0.197
0.067CysPhe: 0.067 ± 0.056
0.733CysGly: 0.733 ± 0.208
0.4CysHis: 0.4 ± 0.151
0.4CysIle: 0.4 ± 0.174
0.2CysLys: 0.2 ± 0.094
0.666CysLeu: 0.666 ± 0.252
0.0CysMet: 0.0 ± 0.0
0.266CysAsn: 0.266 ± 0.127
0.4CysPro: 0.4 ± 0.222
0.333CysGln: 0.333 ± 0.16
0.266CysArg: 0.266 ± 0.126
0.666CysSer: 0.666 ± 0.234
0.666CysThr: 0.666 ± 0.264
0.466CysVal: 0.466 ± 0.173
0.2CysTrp: 0.2 ± 0.142
0.2CysTyr: 0.2 ± 0.124
0.0CysXaa: 0.0 ± 0.0
Asp
6.529AspAla: 6.529 ± 0.772
0.4AspCys: 0.4 ± 0.18
4.197AspAsp: 4.197 ± 0.768
4.863AspGlu: 4.863 ± 0.834
2.065AspPhe: 2.065 ± 0.376
5.863AspGly: 5.863 ± 0.728
1.399AspHis: 1.399 ± 0.346
3.131AspIle: 3.131 ± 0.473
2.465AspLys: 2.465 ± 0.445
5.33AspLeu: 5.33 ± 0.62
1.532AspMet: 1.532 ± 0.319
1.399AspAsn: 1.399 ± 0.302
4.197AspPro: 4.197 ± 0.511
2.265AspGln: 2.265 ± 0.443
4.597AspArg: 4.597 ± 0.577
2.665AspSer: 2.665 ± 0.529
3.864AspThr: 3.864 ± 0.564
4.264AspVal: 4.264 ± 0.553
1.266AspTrp: 1.266 ± 0.286
1.532AspTyr: 1.532 ± 0.333
0.0AspXaa: 0.0 ± 0.0
Glu
7.595GluAla: 7.595 ± 0.857
1.133GluCys: 1.133 ± 0.28
4.131GluAsp: 4.131 ± 0.582
4.064GluGlu: 4.064 ± 0.763
2.199GluPhe: 2.199 ± 0.394
5.796GluGly: 5.796 ± 0.758
1.266GluHis: 1.266 ± 0.317
2.798GluIle: 2.798 ± 0.449
2.598GluLys: 2.598 ± 0.414
6.063GluLeu: 6.063 ± 0.731
1.399GluMet: 1.399 ± 0.323
1.865GluAsn: 1.865 ± 0.35
2.865GluPro: 2.865 ± 0.469
2.598GluGln: 2.598 ± 0.386
4.197GluArg: 4.197 ± 0.587
3.664GluSer: 3.664 ± 0.564
3.864GluThr: 3.864 ± 0.572
4.93GluVal: 4.93 ± 0.602
1.532GluTrp: 1.532 ± 0.352
1.799GluTyr: 1.799 ± 0.382
0.0GluXaa: 0.0 ± 0.0
Phe
2.931PheAla: 2.931 ± 0.38
0.133PheCys: 0.133 ± 0.1
2.665PheAsp: 2.665 ± 0.442
2.265PheGlu: 2.265 ± 0.445
1.199PhePhe: 1.199 ± 0.276
3.131PheGly: 3.131 ± 0.398
0.333PheHis: 0.333 ± 0.164
1.266PheIle: 1.266 ± 0.252
1.266PheLys: 1.266 ± 0.345
2.199PheLeu: 2.199 ± 0.503
0.999PheMet: 0.999 ± 0.246
0.999PheAsn: 0.999 ± 0.235
1.266PhePro: 1.266 ± 0.252
0.466PheGln: 0.466 ± 0.167
2.132PheArg: 2.132 ± 0.367
1.999PheSer: 1.999 ± 0.337
1.932PheThr: 1.932 ± 0.298
2.065PheVal: 2.065 ± 0.41
0.799PheTrp: 0.799 ± 0.191
0.933PheTyr: 0.933 ± 0.243
0.0PheXaa: 0.0 ± 0.0
Gly
7.462GlyAla: 7.462 ± 1.087
0.6GlyCys: 0.6 ± 0.263
5.996GlyAsp: 5.996 ± 0.98
4.064GlyGlu: 4.064 ± 0.663
3.065GlyPhe: 3.065 ± 0.525
6.662GlyGly: 6.662 ± 0.621
2.132GlyHis: 2.132 ± 0.434
3.797GlyIle: 3.797 ± 0.719
4.664GlyLys: 4.664 ± 0.521
6.396GlyLeu: 6.396 ± 0.656
1.865GlyMet: 1.865 ± 0.305
2.598GlyAsn: 2.598 ± 0.382
4.264GlyPro: 4.264 ± 1.35
2.332GlyGln: 2.332 ± 0.426
4.197GlyArg: 4.197 ± 0.636
4.664GlySer: 4.664 ± 0.694
5.796GlyThr: 5.796 ± 0.593
6.662GlyVal: 6.662 ± 0.547
2.265GlyTrp: 2.265 ± 0.44
3.131GlyTyr: 3.131 ± 0.646
0.0GlyXaa: 0.0 ± 0.0
His
1.399HisAla: 1.399 ± 0.352
0.133HisCys: 0.133 ± 0.098
1.133HisAsp: 1.133 ± 0.252
1.199HisGlu: 1.199 ± 0.361
1.066HisPhe: 1.066 ± 0.227
1.266HisGly: 1.266 ± 0.333
0.666HisHis: 0.666 ± 0.191
0.666HisIle: 0.666 ± 0.237
0.2HisLys: 0.2 ± 0.128
1.666HisLeu: 1.666 ± 0.359
0.266HisMet: 0.266 ± 0.113
0.2HisAsn: 0.2 ± 0.113
1.332HisPro: 1.332 ± 0.251
0.666HisGln: 0.666 ± 0.193
1.066HisArg: 1.066 ± 0.298
1.332HisSer: 1.332 ± 0.44
1.399HisThr: 1.399 ± 0.317
1.133HisVal: 1.133 ± 0.34
0.999HisTrp: 0.999 ± 0.275
0.799HisTyr: 0.799 ± 0.238
0.0HisXaa: 0.0 ± 0.0
Ile
5.53IleAla: 5.53 ± 0.71
0.067IleCys: 0.067 ± 0.062
2.665IleAsp: 2.665 ± 0.463
3.531IleGlu: 3.531 ± 0.443
1.133IlePhe: 1.133 ± 0.308
3.598IleGly: 3.598 ± 0.506
0.6IleHis: 0.6 ± 0.242
1.266IleIle: 1.266 ± 0.315
2.665IleLys: 2.665 ± 0.749
2.931IleLeu: 2.931 ± 0.61
0.799IleMet: 0.799 ± 0.251
1.266IleAsn: 1.266 ± 0.288
2.665IlePro: 2.665 ± 0.425
0.866IleGln: 0.866 ± 0.267
2.931IleArg: 2.931 ± 0.416
2.132IleSer: 2.132 ± 0.369
2.665IleThr: 2.665 ± 0.401
3.664IleVal: 3.664 ± 0.492
0.466IleTrp: 0.466 ± 0.174
0.933IleTyr: 0.933 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
5.663LysAla: 5.663 ± 0.796
0.266LysCys: 0.266 ± 0.134
2.265LysAsp: 2.265 ± 0.447
2.199LysGlu: 2.199 ± 0.301
1.199LysPhe: 1.199 ± 0.245
4.797LysGly: 4.797 ± 0.787
0.799LysHis: 0.799 ± 0.211
2.199LysIle: 2.199 ± 0.456
2.598LysLys: 2.598 ± 0.587
4.33LysLeu: 4.33 ± 0.792
1.332LysMet: 1.332 ± 0.247
1.666LysAsn: 1.666 ± 0.381
3.331LysPro: 3.331 ± 0.778
1.332LysGln: 1.332 ± 0.356
2.332LysArg: 2.332 ± 0.378
2.465LysSer: 2.465 ± 0.459
2.865LysThr: 2.865 ± 0.469
3.131LysVal: 3.131 ± 0.602
0.333LysTrp: 0.333 ± 0.144
1.133LysTyr: 1.133 ± 0.278
0.0LysXaa: 0.0 ± 0.0
Leu
9.86LeuAla: 9.86 ± 1.009
0.4LeuCys: 0.4 ± 0.172
5.73LeuAsp: 5.73 ± 0.538
4.73LeuGlu: 4.73 ± 0.524
1.666LeuPhe: 1.666 ± 0.367
7.062LeuGly: 7.062 ± 0.857
1.799LeuHis: 1.799 ± 0.371
3.464LeuIle: 3.464 ± 0.511
4.33LeuLys: 4.33 ± 0.891
6.196LeuLeu: 6.196 ± 0.844
1.466LeuMet: 1.466 ± 0.351
3.464LeuAsn: 3.464 ± 0.42
4.264LeuPro: 4.264 ± 0.444
2.665LeuGln: 2.665 ± 0.43
5.596LeuArg: 5.596 ± 0.764
6.129LeuSer: 6.129 ± 0.633
5.863LeuThr: 5.863 ± 0.623
5.33LeuVal: 5.33 ± 0.562
1.332LeuTrp: 1.332 ± 0.224
2.398LeuTyr: 2.398 ± 0.439
0.0LeuXaa: 0.0 ± 0.0
Met
2.998MetAla: 2.998 ± 0.425
0.067MetCys: 0.067 ± 0.065
0.933MetAsp: 0.933 ± 0.286
0.933MetGlu: 0.933 ± 0.237
0.533MetPhe: 0.533 ± 0.248
1.199MetGly: 1.199 ± 0.318
0.2MetHis: 0.2 ± 0.125
1.199MetIle: 1.199 ± 0.257
0.999MetLys: 0.999 ± 0.253
1.532MetLeu: 1.532 ± 0.359
0.6MetMet: 0.6 ± 0.203
0.866MetAsn: 0.866 ± 0.219
1.266MetPro: 1.266 ± 0.311
0.733MetGln: 0.733 ± 0.303
1.399MetArg: 1.399 ± 0.359
2.398MetSer: 2.398 ± 0.313
1.932MetThr: 1.932 ± 0.324
1.532MetVal: 1.532 ± 0.324
0.266MetTrp: 0.266 ± 0.146
0.4MetTyr: 0.4 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
2.732AsnAla: 2.732 ± 0.484
0.466AsnCys: 0.466 ± 0.19
2.132AsnAsp: 2.132 ± 0.348
1.799AsnGlu: 1.799 ± 0.386
0.533AsnPhe: 0.533 ± 0.206
2.532AsnGly: 2.532 ± 0.4
0.666AsnHis: 0.666 ± 0.217
1.399AsnIle: 1.399 ± 0.295
1.466AsnLys: 1.466 ± 0.345
2.798AsnLeu: 2.798 ± 0.382
0.4AsnMet: 0.4 ± 0.163
0.799AsnAsn: 0.799 ± 0.27
1.466AsnPro: 1.466 ± 0.315
1.532AsnGln: 1.532 ± 0.282
2.199AsnArg: 2.199 ± 0.309
1.332AsnSer: 1.332 ± 0.304
2.465AsnThr: 2.465 ± 0.403
2.132AsnVal: 2.132 ± 0.429
0.6AsnTrp: 0.6 ± 0.189
0.866AsnTyr: 0.866 ± 0.203
0.0AsnXaa: 0.0 ± 0.0
Pro
5.197ProAla: 5.197 ± 0.794
0.466ProCys: 0.466 ± 0.177
3.331ProAsp: 3.331 ± 0.498
5.063ProGlu: 5.063 ± 0.671
1.732ProPhe: 1.732 ± 0.331
4.863ProGly: 4.863 ± 0.624
0.733ProHis: 0.733 ± 0.221
2.132ProIle: 2.132 ± 0.468
2.865ProLys: 2.865 ± 0.616
2.798ProLeu: 2.798 ± 0.466
0.733ProMet: 0.733 ± 0.223
1.066ProAsn: 1.066 ± 0.279
2.598ProPro: 2.598 ± 0.479
1.332ProGln: 1.332 ± 0.44
2.865ProArg: 2.865 ± 0.425
3.464ProSer: 3.464 ± 0.59
4.131ProThr: 4.131 ± 0.615
4.264ProVal: 4.264 ± 0.571
0.999ProTrp: 0.999 ± 0.255
1.266ProTyr: 1.266 ± 0.314
0.0ProXaa: 0.0 ± 0.0
Gln
4.53GlnAla: 4.53 ± 0.6
0.133GlnCys: 0.133 ± 0.096
1.199GlnAsp: 1.199 ± 0.311
1.799GlnGlu: 1.799 ± 0.425
1.066GlnPhe: 1.066 ± 0.231
2.532GlnGly: 2.532 ± 0.676
0.466GlnHis: 0.466 ± 0.168
1.799GlnIle: 1.799 ± 0.405
1.066GlnLys: 1.066 ± 0.269
2.865GlnLeu: 2.865 ± 0.58
0.866GlnMet: 0.866 ± 0.269
1.199GlnAsn: 1.199 ± 0.295
1.133GlnPro: 1.133 ± 0.298
0.6GlnGln: 0.6 ± 0.173
2.398GlnArg: 2.398 ± 0.349
1.732GlnSer: 1.732 ± 0.308
1.799GlnThr: 1.799 ± 0.357
2.665GlnVal: 2.665 ± 0.418
0.6GlnTrp: 0.6 ± 0.185
0.933GlnTyr: 0.933 ± 0.28
0.0GlnXaa: 0.0 ± 0.0
Arg
5.33ArgAla: 5.33 ± 0.603
0.666ArgCys: 0.666 ± 0.316
3.797ArgAsp: 3.797 ± 0.51
5.063ArgGlu: 5.063 ± 0.623
2.398ArgPhe: 2.398 ± 0.382
4.064ArgGly: 4.064 ± 0.471
1.199ArgHis: 1.199 ± 0.266
2.465ArgIle: 2.465 ± 0.328
2.931ArgLys: 2.931 ± 0.493
6.529ArgLeu: 6.529 ± 0.805
1.666ArgMet: 1.666 ± 0.34
2.199ArgAsn: 2.199 ± 0.371
2.465ArgPro: 2.465 ± 0.445
2.132ArgGln: 2.132 ± 0.43
5.863ArgArg: 5.863 ± 0.971
4.064ArgSer: 4.064 ± 0.602
4.131ArgThr: 4.131 ± 0.539
3.931ArgVal: 3.931 ± 0.441
0.999ArgTrp: 0.999 ± 0.306
2.132ArgTyr: 2.132 ± 0.428
0.0ArgXaa: 0.0 ± 0.0
Ser
7.395SerAla: 7.395 ± 1.05
0.4SerCys: 0.4 ± 0.205
3.664SerAsp: 3.664 ± 0.491
4.064SerGlu: 4.064 ± 0.462
2.132SerPhe: 2.132 ± 0.367
4.597SerGly: 4.597 ± 0.605
1.133SerHis: 1.133 ± 0.338
2.598SerIle: 2.598 ± 0.509
2.265SerLys: 2.265 ± 0.341
5.929SerLeu: 5.929 ± 0.683
1.199SerMet: 1.199 ± 0.307
1.865SerAsn: 1.865 ± 0.318
2.665SerPro: 2.665 ± 0.409
2.132SerGln: 2.132 ± 0.367
3.264SerArg: 3.264 ± 0.628
3.398SerSer: 3.398 ± 0.684
4.997SerThr: 4.997 ± 0.667
5.463SerVal: 5.463 ± 0.527
1.332SerTrp: 1.332 ± 0.25
1.399SerTyr: 1.399 ± 0.353
0.0SerXaa: 0.0 ± 0.0
Thr
6.196ThrAla: 6.196 ± 0.619
0.733ThrCys: 0.733 ± 0.197
3.664ThrAsp: 3.664 ± 0.542
4.131ThrGlu: 4.131 ± 0.561
2.865ThrPhe: 2.865 ± 0.457
5.663ThrGly: 5.663 ± 1.368
1.133ThrHis: 1.133 ± 0.289
2.732ThrIle: 2.732 ± 0.391
2.998ThrLys: 2.998 ± 0.467
5.53ThrLeu: 5.53 ± 0.553
1.266ThrMet: 1.266 ± 0.289
1.799ThrAsn: 1.799 ± 0.341
4.53ThrPro: 4.53 ± 1.02
1.666ThrGln: 1.666 ± 0.325
4.064ThrArg: 4.064 ± 0.576
5.263ThrSer: 5.263 ± 0.661
5.13ThrThr: 5.13 ± 0.602
5.396ThrVal: 5.396 ± 0.81
1.466ThrTrp: 1.466 ± 0.313
1.865ThrTyr: 1.865 ± 0.412
0.0ThrXaa: 0.0 ± 0.0
Val
7.062ValAla: 7.062 ± 0.681
0.6ValCys: 0.6 ± 0.18
4.264ValAsp: 4.264 ± 0.461
5.53ValGlu: 5.53 ± 0.618
1.732ValPhe: 1.732 ± 0.298
5.796ValGly: 5.796 ± 0.602
1.599ValHis: 1.599 ± 0.349
3.598ValIle: 3.598 ± 0.498
3.797ValLys: 3.797 ± 0.69
5.796ValLeu: 5.796 ± 0.72
1.599ValMet: 1.599 ± 0.344
1.799ValAsn: 1.799 ± 0.425
4.131ValPro: 4.131 ± 0.49
2.665ValGln: 2.665 ± 0.481
4.664ValArg: 4.664 ± 0.59
4.863ValSer: 4.863 ± 0.562
5.996ValThr: 5.996 ± 0.68
4.863ValVal: 4.863 ± 0.627
0.933ValTrp: 0.933 ± 0.214
1.999ValTyr: 1.999 ± 0.417
0.0ValXaa: 0.0 ± 0.0
Trp
2.199TrpAla: 2.199 ± 0.397
0.4TrpCys: 0.4 ± 0.156
1.999TrpAsp: 1.999 ± 0.393
0.999TrpGlu: 0.999 ± 0.228
0.466TrpPhe: 0.466 ± 0.188
1.266TrpGly: 1.266 ± 0.302
0.2TrpHis: 0.2 ± 0.119
0.466TrpIle: 0.466 ± 0.165
1.532TrpLys: 1.532 ± 0.256
1.732TrpLeu: 1.732 ± 0.311
0.466TrpMet: 0.466 ± 0.153
0.533TrpAsn: 0.533 ± 0.209
0.666TrpPro: 0.666 ± 0.221
0.333TrpGln: 0.333 ± 0.159
1.133TrpArg: 1.133 ± 0.274
1.466TrpSer: 1.466 ± 0.329
1.266TrpThr: 1.266 ± 0.263
1.732TrpVal: 1.732 ± 0.338
0.2TrpTrp: 0.2 ± 0.123
0.333TrpTyr: 0.333 ± 0.153
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.865TyrAla: 2.865 ± 0.347
0.2TyrCys: 0.2 ± 0.118
2.398TyrAsp: 2.398 ± 0.443
1.865TyrGlu: 1.865 ± 0.362
0.6TyrPhe: 0.6 ± 0.195
3.198TyrGly: 3.198 ± 0.55
0.466TyrHis: 0.466 ± 0.187
0.866TyrIle: 0.866 ± 0.207
0.799TyrLys: 0.799 ± 0.296
1.932TyrLeu: 1.932 ± 0.353
0.666TyrMet: 0.666 ± 0.214
0.999TyrAsn: 0.999 ± 0.206
1.399TyrPro: 1.399 ± 0.36
0.933TyrGln: 0.933 ± 0.23
2.465TyrArg: 2.465 ± 0.422
2.332TyrSer: 2.332 ± 0.501
1.266TyrThr: 1.266 ± 0.266
1.599TyrVal: 1.599 ± 0.299
0.6TyrTrp: 0.6 ± 0.213
0.6TyrTyr: 0.6 ± 0.2
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (15011 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski