Clostridium phage slur17

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Leicestervirus; unclassified Leicestervirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J1JB41|A0A1J1JB41_9CAUD Uncharacterized protein OS=Clostridium phage slur17 OX=1720506 PE=4 SV=1
MM1 pKa = 7.64SEE3 pKa = 5.36FVQCCCCEE11 pKa = 3.56RR12 pKa = 11.84TINIEE17 pKa = 4.12EE18 pKa = 4.26NNHH21 pKa = 3.91VQYY24 pKa = 10.83EE25 pKa = 4.24KK26 pKa = 10.83EE27 pKa = 3.88ALGLVFTLYY36 pKa = 10.73FCLNCVDD43 pKa = 4.63EE44 pKa = 4.67LSEE47 pKa = 4.15MEE49 pKa = 4.11

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J1J901|A0A1J1J901_9CAUD Uncharacterized protein OS=Clostridium phage slur17 OX=1720506 PE=4 SV=1
MM1 pKa = 7.59GSKK4 pKa = 10.3KK5 pKa = 9.71PANPVKK11 pKa = 10.44DD12 pKa = 3.12KK13 pKa = 11.51RR14 pKa = 11.84MVLNIQEE21 pKa = 4.17YY22 pKa = 10.29LKK24 pKa = 10.55EE25 pKa = 4.15KK26 pKa = 10.25SVRR29 pKa = 11.84NYY31 pKa = 9.95VLFVLGVATGYY42 pKa = 10.5RR43 pKa = 11.84AGDD46 pKa = 3.56LVKK49 pKa = 10.86LQVRR53 pKa = 11.84DD54 pKa = 3.39VRR56 pKa = 11.84NAIDD60 pKa = 3.33EE61 pKa = 4.83GYY63 pKa = 10.33FLIMEE68 pKa = 4.42SKK70 pKa = 10.46KK71 pKa = 10.85EE72 pKa = 3.67KK73 pKa = 9.45TKK75 pKa = 10.91NIRR78 pKa = 11.84KK79 pKa = 9.46KK80 pKa = 10.22NKK82 pKa = 9.08KK83 pKa = 8.24PRR85 pKa = 11.84KK86 pKa = 9.44APIVPNLEE94 pKa = 4.13RR95 pKa = 11.84VLKK98 pKa = 10.75SYY100 pKa = 10.65IRR102 pKa = 11.84DD103 pKa = 3.65KK104 pKa = 11.22KK105 pKa = 10.49DD106 pKa = 3.2YY107 pKa = 10.69EE108 pKa = 4.38YY109 pKa = 10.63MFPSRR114 pKa = 11.84QKK116 pKa = 10.77SVTPHH121 pKa = 5.98IGVEE125 pKa = 3.98RR126 pKa = 11.84VTVILKK132 pKa = 9.26EE133 pKa = 3.7AGRR136 pKa = 11.84YY137 pKa = 8.72FGLKK141 pKa = 10.0HH142 pKa = 5.2ITAHH146 pKa = 5.93SMRR149 pKa = 11.84KK150 pKa = 7.02TYY152 pKa = 10.56AYY154 pKa = 9.02TIYY157 pKa = 10.25EE158 pKa = 3.97EE159 pKa = 4.35SGFDD163 pKa = 3.53IIRR166 pKa = 11.84VKK168 pKa = 10.9EE169 pKa = 4.06MLGHH173 pKa = 6.34SSIEE177 pKa = 3.92EE178 pKa = 4.01TKK180 pKa = 10.79VYY182 pKa = 10.74LGLNEE187 pKa = 4.08EE188 pKa = 4.61QYY190 pKa = 10.82QEE192 pKa = 3.98YY193 pKa = 11.35SMFLNDD199 pKa = 6.09LIGG202 pKa = 3.77

Molecular weight: 23.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11689

36

1767

229.2

26.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.681 ± 0.343

0.95 ± 0.147

5.86 ± 0.225

8.854 ± 0.372

4.201 ± 0.306

6.057 ± 0.439

0.924 ± 0.155

8.914 ± 0.248

9.975 ± 0.459

7.939 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.301 ± 0.142

7.349 ± 0.359

1.762 ± 0.136

3.071 ± 0.195

3.422 ± 0.295

6.399 ± 0.295

5.672 ± 0.386

5.509 ± 0.238

1.009 ± 0.116

4.149 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski