Sphaerotilus phage vB_SnaP-R1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; unclassified Krylovirinae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9SXV4|A0A6B9SXV4_9CAUD Acetyltransferase OS=Sphaerotilus phage vB_SnaP-R1 OX=2696336 GN=SnaR1_gp30 PE=4 SV=1
MM1 pKa = 7.44SAIQMAVLLMVNGGPTPFNFTPTISADD28 pKa = 3.31TLNYY32 pKa = 9.96NLRR35 pKa = 11.84AAAVAAGWNQTDD47 pKa = 4.03PLNATVTINSGVYY60 pKa = 9.45VGSSTTSGYY69 pKa = 11.36GFDD72 pKa = 3.66TGVTFPAGTTLAVINNGFIVGCGGKK97 pKa = 10.58GGDD100 pKa = 3.89SPDD103 pKa = 3.38GVGAYY108 pKa = 9.64AGSPGGPAFRR118 pKa = 11.84AQAAVTVTNNGTIGGGGGGGGGGGPEE144 pKa = 4.1AYY146 pKa = 9.92GGSVDD151 pKa = 3.71QGGGGGGGGRR161 pKa = 11.84GLNGGAGGAGGSNLGNGAAGSKK183 pKa = 9.87AAAGSGAGAGGSGPEE198 pKa = 4.14GEE200 pKa = 4.55GAGGNGGGLGSAGSPGSPGRR220 pKa = 11.84DD221 pKa = 2.88WEE223 pKa = 4.67GGSTAGGAGGAAGAAVVGNSNITWAATGTRR253 pKa = 11.84LGSITT258 pKa = 3.81

Molecular weight: 23.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9SUK1|A0A6B9SUK1_9CAUD Internal virion protein OS=Sphaerotilus phage vB_SnaP-R1 OX=2696336 GN=SnaR1_gp40 PE=4 SV=1
MM1 pKa = 7.64SDD3 pKa = 2.99KK4 pKa = 10.51TKK6 pKa = 10.14HH7 pKa = 4.24QRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84YY12 pKa = 8.5AAEE15 pKa = 3.66HH16 pKa = 6.39KK17 pKa = 9.67FAKK20 pKa = 10.16FAALNGLIDD29 pKa = 4.01QGGEE33 pKa = 3.76FAKK36 pKa = 10.54DD37 pKa = 2.98GRR39 pKa = 11.84KK40 pKa = 9.4ARR42 pKa = 11.84RR43 pKa = 11.84KK44 pKa = 8.93MADD47 pKa = 2.46SWSRR51 pKa = 11.84RR52 pKa = 11.84GGRR55 pKa = 11.84NVEE58 pKa = 4.25GFYY61 pKa = 10.79KK62 pKa = 10.45PQLAKK67 pKa = 10.61AAA69 pKa = 4.12

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

13105

36

1186

262.1

28.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.919 ± 0.53

1.129 ± 0.215

5.677 ± 0.235

5.837 ± 0.313

3.136 ± 0.2

8.287 ± 0.592

1.992 ± 0.223

4.212 ± 0.183

4.861 ± 0.31

8.714 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.152

3.586 ± 0.21

4.51 ± 0.293

4.578 ± 0.296

6.166 ± 0.321

5.609 ± 0.394

6.387 ± 0.652

6.746 ± 0.233

1.572 ± 0.136

2.808 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski