Circoviridae 2 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TNA9|S5TNA9_9CIRC ATP-dependent helicase Rep OS=Circoviridae 2 LDMD-2013 OX=1379695 PE=3 SV=1
MM1 pKa = 7.47PRR3 pKa = 11.84AKK5 pKa = 9.83RR6 pKa = 11.84VRR8 pKa = 11.84NQEE11 pKa = 3.92GEE13 pKa = 4.28VEE15 pKa = 4.3DD16 pKa = 4.34PVAKK20 pKa = 10.22GRR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 10.57GMWASMKK31 pKa = 10.26LSKK34 pKa = 10.36AQPWDD39 pKa = 3.3TYY41 pKa = 9.29GARR44 pKa = 11.84RR45 pKa = 11.84VQRR48 pKa = 11.84GTPEE52 pKa = 3.8NLEE55 pKa = 4.17LFGPTWKK62 pKa = 10.4EE63 pKa = 3.02ADD65 pKa = 3.28EE66 pKa = 4.32MQRR69 pKa = 11.84SARR72 pKa = 11.84KK73 pKa = 8.54EE74 pKa = 3.6FGYY77 pKa = 10.14SGRR80 pKa = 11.84GLYY83 pKa = 10.52GPLAYY88 pKa = 10.1AAGAGIDD95 pKa = 3.72YY96 pKa = 10.56AVKK99 pKa = 10.37NPRR102 pKa = 11.84ATRR105 pKa = 11.84SFFSKK110 pKa = 10.35AFKK113 pKa = 10.53GRR115 pKa = 11.84GMYY118 pKa = 9.12TGRR121 pKa = 11.84GMYY124 pKa = 9.0TGRR127 pKa = 11.84GEE129 pKa = 4.07YY130 pKa = 8.72TASNEE135 pKa = 3.84LMAGSTSSPPMFQSAGDD152 pKa = 3.53EE153 pKa = 4.24AGALMVSHH161 pKa = 6.99RR162 pKa = 11.84EE163 pKa = 3.83YY164 pKa = 11.09VGDD167 pKa = 3.81IFAPAAADD175 pKa = 3.37VSKK178 pKa = 9.62FTVQSFPLNPGLEE191 pKa = 4.09QTFPWLSQIAQNYY204 pKa = 8.25EE205 pKa = 3.89EE206 pKa = 5.46YY207 pKa = 10.45EE208 pKa = 4.0LKK210 pKa = 10.34QCVFEE215 pKa = 4.72FVSTVQDD222 pKa = 3.24INSTNGQVGTIITATQYY239 pKa = 11.13NPSEE243 pKa = 4.18GDD245 pKa = 3.56FTDD248 pKa = 3.93KK249 pKa = 10.8PAMAAYY255 pKa = 9.24AHH257 pKa = 6.07SVSGKK262 pKa = 8.42STDD265 pKa = 3.55NQTHH269 pKa = 5.46GVEE272 pKa = 4.32CDD274 pKa = 3.17PAKK277 pKa = 10.86LSGAEE282 pKa = 3.98GKK284 pKa = 9.3YY285 pKa = 10.13VRR287 pKa = 11.84ANPVMTGEE295 pKa = 4.06DD296 pKa = 3.85LKK298 pKa = 10.38TYY300 pKa = 10.76DD301 pKa = 3.44HH302 pKa = 6.93GRR304 pKa = 11.84FQLATHH310 pKa = 7.11NIPAAMASGTLGEE323 pKa = 4.96LYY325 pKa = 10.41VAYY328 pKa = 8.31TVCLRR333 pKa = 11.84KK334 pKa = 9.65PKK336 pKa = 10.32FFVGRR341 pKa = 11.84GLGLTRR347 pKa = 11.84YY348 pKa = 9.97CEE350 pKa = 4.13VWDD353 pKa = 3.89ATASGSTTDD362 pKa = 3.49GDD364 pKa = 3.96HH365 pKa = 7.47PYY367 pKa = 10.89GADD370 pKa = 3.38GEE372 pKa = 4.53QFLGKK377 pKa = 10.06QNNIAIKK384 pKa = 8.37TVKK387 pKa = 7.93TTDD390 pKa = 2.98TLEE393 pKa = 4.17IVFPAYY399 pKa = 10.59YY400 pKa = 10.4GGNLEE405 pKa = 4.43IKK407 pKa = 9.83LANEE411 pKa = 4.29GTLSSGTFCGEE422 pKa = 4.23LVASMAVEE430 pKa = 4.53GNCVLVKK437 pKa = 10.47DD438 pKa = 5.36LYY440 pKa = 11.53ASGPSNTDD448 pKa = 2.3SPAASRR454 pKa = 11.84SAGDD458 pKa = 3.93ANNSHH463 pKa = 7.25AIIHH467 pKa = 5.46VRR469 pKa = 11.84VSPSTNAVDD478 pKa = 3.47NKK480 pKa = 10.94LIITSGQGSVFNVNQSMIDD499 pKa = 3.27VTEE502 pKa = 4.12YY503 pKa = 9.74NTYY506 pKa = 10.49GQDD509 pKa = 3.11TLEE512 pKa = 4.59LVNSAGTSVVIAA524 pKa = 4.46

Molecular weight: 56.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5TNA9|S5TNA9_9CIRC ATP-dependent helicase Rep OS=Circoviridae 2 LDMD-2013 OX=1379695 PE=3 SV=1
MM1 pKa = 7.83KK2 pKa = 10.21IPDD5 pKa = 3.71RR6 pKa = 11.84SRR8 pKa = 11.84HH9 pKa = 4.69RR10 pKa = 11.84AYY12 pKa = 10.9CITLNNYY19 pKa = 9.35FEE21 pKa = 5.63HH22 pKa = 7.53DD23 pKa = 3.66RR24 pKa = 11.84QNLEE28 pKa = 3.96TLVEE32 pKa = 4.08RR33 pKa = 11.84GIAKK37 pKa = 7.95YY38 pKa = 10.91VCFQPEE44 pKa = 4.23KK45 pKa = 10.95APGTGTRR52 pKa = 11.84HH53 pKa = 4.36IQGYY57 pKa = 7.56VVFQNARR64 pKa = 11.84TFSGAKK70 pKa = 10.08AIIGEE75 pKa = 4.36GAHH78 pKa = 6.28IEE80 pKa = 4.22VARR83 pKa = 11.84GSSQANIEE91 pKa = 4.2YY92 pKa = 10.21CSKK95 pKa = 11.07DD96 pKa = 3.3EE97 pKa = 5.09SRR99 pKa = 11.84DD100 pKa = 3.51TEE102 pKa = 4.08AGFGFIEE109 pKa = 4.27RR110 pKa = 11.84GNRR113 pKa = 11.84EE114 pKa = 4.17DD115 pKa = 3.84VLGTGSGGGTRR126 pKa = 11.84TDD128 pKa = 3.71LAVVAASLRR137 pKa = 11.84TGATLSQIAEE147 pKa = 4.33DD148 pKa = 4.5HH149 pKa = 5.97PASYY153 pKa = 11.01IMYY156 pKa = 8.6TRR158 pKa = 11.84GIHH161 pKa = 7.01SYY163 pKa = 10.93AQLSLPKK170 pKa = 9.46RR171 pKa = 11.84SHH173 pKa = 5.2KK174 pKa = 9.76TIVHH178 pKa = 6.56WYY180 pKa = 7.38WGPTGTGKK188 pKa = 8.08TRR190 pKa = 11.84LACEE194 pKa = 4.37EE195 pKa = 4.5SPDD198 pKa = 4.22AYY200 pKa = 9.3WKK202 pKa = 10.77SSAHH206 pKa = 4.65QWYY209 pKa = 10.03DD210 pKa = 3.28GYY212 pKa = 11.46DD213 pKa = 3.4GLADD217 pKa = 5.13IIIDD221 pKa = 4.05DD222 pKa = 4.15YY223 pKa = 11.45RR224 pKa = 11.84CSFSTFNEE232 pKa = 3.88LLRR235 pKa = 11.84LLDD238 pKa = 4.26RR239 pKa = 11.84YY240 pKa = 9.93PYY242 pKa = 9.02QAQVKK247 pKa = 9.95GGTIHH252 pKa = 6.74VNAKK256 pKa = 10.08RR257 pKa = 11.84IFITAPKK264 pKa = 9.43DD265 pKa = 3.5PRR267 pKa = 11.84SMWQSRR273 pKa = 11.84TEE275 pKa = 3.64EE276 pKa = 5.05DD277 pKa = 2.87IAQLEE282 pKa = 4.08RR283 pKa = 11.84RR284 pKa = 11.84IEE286 pKa = 3.92VVRR289 pKa = 11.84YY290 pKa = 8.92FGVDD294 pKa = 3.25EE295 pKa = 5.26NIPEE299 pKa = 4.31PVLNEE304 pKa = 3.86APAVIHH310 pKa = 6.57GFDD313 pKa = 3.93PGVALLL319 pKa = 4.31

Molecular weight: 35.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

843

319

524

421.5

46.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.727 ± 0.55

1.305 ± 0.152

5.101 ± 0.314

6.287 ± 0.353

3.677 ± 0.133

9.727 ± 0.55

2.254 ± 0.692

4.745 ± 1.429

4.389 ± 0.364

5.813 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.017 ± 0.624

4.27 ± 0.658

4.508 ± 0.069

3.796 ± 0.02

5.813 ± 1.173

7.236 ± 0.56

7.117 ± 0.491

6.287 ± 0.555

1.186 ± 0.221

4.745 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski