Paenibacillus sp. 18JY21-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4798 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4E8B4|A0A4R4E8B4_9BACL Peptidylprolyl isomerase OS=Paenibacillus sp. 18JY21-1 OX=2545760 GN=E0485_19700 PE=4 SV=1
MM1 pKa = 7.06EE2 pKa = 4.23QQHH5 pKa = 5.83EE6 pKa = 5.12VICPWCQSEE15 pKa = 4.27IHH17 pKa = 6.94WDD19 pKa = 3.69PEE21 pKa = 3.7IGPEE25 pKa = 4.94DD26 pKa = 3.78MCPHH30 pKa = 7.06CFNEE34 pKa = 4.07LGDD37 pKa = 3.78YY38 pKa = 10.74RR39 pKa = 11.84SIKK42 pKa = 10.66VGMDD46 pKa = 3.27TLDD49 pKa = 3.82EE50 pKa = 4.31EE51 pKa = 5.47VDD53 pKa = 5.35DD54 pKa = 4.93EE55 pKa = 6.03DD56 pKa = 6.45LDD58 pKa = 4.17EE59 pKa = 5.14TDD61 pKa = 4.04DD62 pKa = 3.81TLDD65 pKa = 3.99PVEE68 pKa = 5.27TDD70 pKa = 5.81DD71 pKa = 5.31DD72 pKa = 4.01WEE74 pKa = 5.14DD75 pKa = 3.53NLEE78 pKa = 4.01EE79 pKa = 4.06DD80 pKa = 4.48TYY82 pKa = 11.49SINAQACIDD91 pKa = 3.97EE92 pKa = 4.44QEE94 pKa = 4.37EE95 pKa = 4.28APEE98 pKa = 4.12CLNCRR103 pKa = 11.84DD104 pKa = 4.09LLLLTGEE111 pKa = 4.25RR112 pKa = 11.84TVTAEE117 pKa = 4.08EE118 pKa = 4.43FTGHH122 pKa = 5.63VPASLGKK129 pKa = 10.26QILPAPFTVQFYY141 pKa = 10.67VCPQCFRR148 pKa = 11.84TEE150 pKa = 4.12QILSDD155 pKa = 3.8KK156 pKa = 10.79DD157 pKa = 3.4RR158 pKa = 11.84LGMIEE163 pKa = 5.56RR164 pKa = 11.84LSQDD168 pKa = 2.55IQKK171 pKa = 10.93

Molecular weight: 19.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4E9S9|A0A4R4E9S9_9BACL Class C beta-lactamase-related serine hydrolase OS=Paenibacillus sp. 18JY21-1 OX=2545760 GN=E0485_13565 PE=4 SV=1
MM1 pKa = 7.61GPTFQPNTRR10 pKa = 11.84KK11 pKa = 9.87RR12 pKa = 11.84KK13 pKa = 8.69KK14 pKa = 9.13NHH16 pKa = 5.02GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATVNGRR28 pKa = 11.84KK29 pKa = 9.19VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4798

0

4798

1458165

24

3638

303.9

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.462 ± 0.04

0.808 ± 0.01

5.164 ± 0.029

6.529 ± 0.04

4.193 ± 0.027

6.924 ± 0.04

2.136 ± 0.019

7.942 ± 0.038

5.861 ± 0.035

9.982 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.983 ± 0.019

4.275 ± 0.026

3.689 ± 0.024

3.989 ± 0.025

4.456 ± 0.028

6.368 ± 0.029

5.499 ± 0.031

7.005 ± 0.029

1.141 ± 0.014

3.594 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski