Paramecium bursaria Chlorella virus NY2A (PBCV-NY2A)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Chlorovirus

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 873 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7IVT5|A7IVT5_PBCVN Uncharacterized protein b060L OS=Paramecium bursaria Chlorella virus NY2A OX=46021 GN=b060L PE=4 SV=1
MM1 pKa = 7.09KK2 pKa = 9.62TDD4 pKa = 3.24ATIFPIVCSCPDD16 pKa = 2.78ICVFAFTLSSPVDD29 pKa = 3.28TFAAFTFPVADD40 pKa = 4.11TFPVISVEE48 pKa = 4.08IVPVALPTFAFVIRR62 pKa = 11.84DD63 pKa = 3.86PLPP66 pKa = 3.88

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7IWV4|A7IWV4_PBCVN Uncharacterized protein B429R OS=Paramecium bursaria Chlorella virus NY2A OX=46021 GN=B429R PE=4 SV=1
MM1 pKa = 7.55CGNRR5 pKa = 11.84SFRR8 pKa = 11.84WNRR11 pKa = 11.84DD12 pKa = 2.99SEE14 pKa = 4.88HH15 pKa = 6.23IQSHH19 pKa = 5.29EE20 pKa = 4.16KK21 pKa = 10.68SRR23 pKa = 11.84DD24 pKa = 3.15ACVVHH29 pKa = 6.99RR30 pKa = 11.84NRR32 pKa = 11.84IRR34 pKa = 11.84LEE36 pKa = 4.44DD37 pKa = 3.21GHH39 pKa = 6.45CRR41 pKa = 11.84HH42 pKa = 6.71RR43 pKa = 11.84EE44 pKa = 3.79RR45 pKa = 11.84IQSNRR50 pKa = 11.84RR51 pKa = 11.84EE52 pKa = 4.05NRR54 pKa = 11.84IEE56 pKa = 3.82EE57 pKa = 4.08CRR59 pKa = 11.84KK60 pKa = 9.36RR61 pKa = 11.84NGRR64 pKa = 11.84SWYY67 pKa = 9.84AKK69 pKa = 9.7RR70 pKa = 11.84IRR72 pKa = 11.84CHH74 pKa = 4.58EE75 pKa = 4.45HH76 pKa = 5.08RR77 pKa = 11.84CEE79 pKa = 4.32WIGVRR84 pKa = 11.84HH85 pKa = 5.43LRR87 pKa = 11.84KK88 pKa = 10.17DD89 pKa = 3.51EE90 pKa = 3.69VRR92 pKa = 11.84ASHH95 pKa = 6.04RR96 pKa = 11.84HH97 pKa = 4.22RR98 pKa = 11.84PYY100 pKa = 10.3RR101 pKa = 11.84SEE103 pKa = 4.49DD104 pKa = 3.17STIRR108 pKa = 11.84TQKK111 pKa = 8.75RR112 pKa = 11.84QYY114 pKa = 10.66RR115 pKa = 11.84STCIVQDD122 pKa = 3.86DD123 pKa = 3.99EE124 pKa = 5.47RR125 pKa = 11.84SGGEE129 pKa = 3.7EE130 pKa = 4.01RR131 pKa = 11.84II132 pKa = 3.99

Molecular weight: 16.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

873

0

873

156815

65

1612

179.6

20.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.01 ± 0.117

2.039 ± 0.078

5.614 ± 0.111

5.096 ± 0.124

5.589 ± 0.097

5.321 ± 0.166

2.516 ± 0.075

7.71 ± 0.095

6.855 ± 0.194

7.505 ± 0.123

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.899 ± 0.06

5.458 ± 0.149

4.348 ± 0.157

2.665 ± 0.072

5.819 ± 0.135

8.003 ± 0.114

6.141 ± 0.119

6.629 ± 0.116

1.062 ± 0.038

3.722 ± 0.075
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski