Amino acid dipepetide frequency for Mycobacterium phage Oscar

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.639AlaAla: 23.639 ± 1.855
1.101AlaCys: 1.101 ± 0.302
8.648AlaAsp: 8.648 ± 0.691
9.539AlaGlu: 9.539 ± 1.039
3.669AlaPhe: 3.669 ± 0.538
9.434AlaGly: 9.434 ± 1.103
2.83AlaHis: 2.83 ± 0.33
4.77AlaIle: 4.77 ± 0.52
5.084AlaLys: 5.084 ± 0.532
11.95AlaLeu: 11.95 ± 0.714
3.04AlaMet: 3.04 ± 0.394
3.04AlaAsn: 3.04 ± 0.597
6.971AlaPro: 6.971 ± 0.791
4.508AlaGln: 4.508 ± 0.594
8.386AlaArg: 8.386 ± 0.736
6.342AlaSer: 6.342 ± 0.783
7.757AlaThr: 7.757 ± 0.864
10.116AlaVal: 10.116 ± 0.766
2.359AlaTrp: 2.359 ± 0.254
3.092AlaTyr: 3.092 ± 0.385
0.0AlaXaa: 0.0 ± 0.0
Cys
1.415CysAla: 1.415 ± 0.325
0.052CysCys: 0.052 ± 0.05
0.839CysAsp: 0.839 ± 0.246
0.891CysGlu: 0.891 ± 0.237
0.21CysPhe: 0.21 ± 0.116
1.258CysGly: 1.258 ± 0.309
0.262CysHis: 0.262 ± 0.114
0.314CysIle: 0.314 ± 0.105
0.524CysLys: 0.524 ± 0.215
0.734CysLeu: 0.734 ± 0.187
0.367CysMet: 0.367 ± 0.12
0.367CysAsn: 0.367 ± 0.161
0.629CysPro: 0.629 ± 0.204
0.052CysGln: 0.052 ± 0.053
0.681CysArg: 0.681 ± 0.184
0.996CysSer: 0.996 ± 0.201
0.891CysThr: 0.891 ± 0.253
0.524CysVal: 0.524 ± 0.144
0.577CysTrp: 0.577 ± 0.181
0.262CysTyr: 0.262 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
7.914AspAla: 7.914 ± 0.714
0.681AspCys: 0.681 ± 0.188
5.399AspAsp: 5.399 ± 0.757
5.818AspGlu: 5.818 ± 0.646
0.891AspPhe: 0.891 ± 0.203
7.233AspGly: 7.233 ± 0.647
1.31AspHis: 1.31 ± 0.276
1.415AspIle: 1.415 ± 0.242
1.468AspLys: 1.468 ± 0.3
6.132AspLeu: 6.132 ± 0.699
1.887AspMet: 1.887 ± 0.352
1.572AspAsn: 1.572 ± 0.274
3.774AspPro: 3.774 ± 0.435
1.782AspGln: 1.782 ± 0.259
4.508AspArg: 4.508 ± 0.636
2.673AspSer: 2.673 ± 0.406
2.726AspThr: 2.726 ± 0.364
4.612AspVal: 4.612 ± 0.516
0.734AspTrp: 0.734 ± 0.179
1.572AspTyr: 1.572 ± 0.316
0.0AspXaa: 0.0 ± 0.0
Glu
7.181GluAla: 7.181 ± 0.895
0.943GluCys: 0.943 ± 0.247
2.516GluAsp: 2.516 ± 0.429
1.101GluGlu: 1.101 ± 0.219
1.834GluPhe: 1.834 ± 0.333
5.294GluGly: 5.294 ± 0.507
1.73GluHis: 1.73 ± 0.311
2.044GluIle: 2.044 ± 0.335
1.468GluLys: 1.468 ± 0.392
5.766GluLeu: 5.766 ± 0.649
1.468GluMet: 1.468 ± 0.245
0.734GluAsn: 0.734 ± 0.205
3.145GluPro: 3.145 ± 0.423
2.359GluGln: 2.359 ± 0.347
5.189GluArg: 5.189 ± 0.578
2.673GluSer: 2.673 ± 0.372
2.254GluThr: 2.254 ± 0.304
5.608GluVal: 5.608 ± 0.727
1.31GluTrp: 1.31 ± 0.289
2.097GluTyr: 2.097 ± 0.411
0.0GluXaa: 0.0 ± 0.0
Phe
3.774PheAla: 3.774 ± 0.618
0.21PheCys: 0.21 ± 0.104
2.516PheAsp: 2.516 ± 0.362
1.31PheGlu: 1.31 ± 0.222
0.524PhePhe: 0.524 ± 0.163
3.092PheGly: 3.092 ± 0.348
0.262PheHis: 0.262 ± 0.118
0.839PheIle: 0.839 ± 0.148
0.943PheLys: 0.943 ± 0.337
1.939PheLeu: 1.939 ± 0.273
0.681PheMet: 0.681 ± 0.168
1.101PheAsn: 1.101 ± 0.291
1.258PhePro: 1.258 ± 0.257
0.681PheGln: 0.681 ± 0.183
1.101PheArg: 1.101 ± 0.234
0.996PheSer: 0.996 ± 0.27
1.415PheThr: 1.415 ± 0.335
2.463PheVal: 2.463 ± 0.35
0.419PheTrp: 0.419 ± 0.128
0.472PheTyr: 0.472 ± 0.196
0.0PheXaa: 0.0 ± 0.0
Gly
10.745GlyAla: 10.745 ± 1.451
1.153GlyCys: 1.153 ± 0.304
5.137GlyAsp: 5.137 ± 0.484
5.294GlyGlu: 5.294 ± 0.426
2.463GlyPhe: 2.463 ± 0.46
10.221GlyGly: 10.221 ± 1.599
1.834GlyHis: 1.834 ± 0.337
3.407GlyIle: 3.407 ± 0.409
3.721GlyLys: 3.721 ± 0.549
6.919GlyLeu: 6.919 ± 0.788
1.939GlyMet: 1.939 ± 0.32
2.83GlyAsn: 2.83 ± 0.336
3.197GlyPro: 3.197 ± 0.389
2.411GlyGln: 2.411 ± 0.442
6.132GlyArg: 6.132 ± 0.655
5.451GlySer: 5.451 ± 0.614
6.499GlyThr: 6.499 ± 0.519
7.128GlyVal: 7.128 ± 0.608
2.201GlyTrp: 2.201 ± 0.306
2.306GlyTyr: 2.306 ± 0.389
0.0GlyXaa: 0.0 ± 0.0
His
2.463HisAla: 2.463 ± 0.444
0.524HisCys: 0.524 ± 0.155
1.572HisAsp: 1.572 ± 0.346
1.468HisGlu: 1.468 ± 0.312
0.629HisPhe: 0.629 ± 0.168
2.254HisGly: 2.254 ± 0.347
0.734HisHis: 0.734 ± 0.187
0.577HisIle: 0.577 ± 0.172
0.419HisLys: 0.419 ± 0.153
2.097HisLeu: 2.097 ± 0.249
0.524HisMet: 0.524 ± 0.159
0.577HisAsn: 0.577 ± 0.138
1.31HisPro: 1.31 ± 0.249
0.419HisGln: 0.419 ± 0.129
2.149HisArg: 2.149 ± 0.366
0.839HisSer: 0.839 ± 0.228
1.363HisThr: 1.363 ± 0.27
1.992HisVal: 1.992 ± 0.276
0.419HisTrp: 0.419 ± 0.152
0.629HisTyr: 0.629 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
6.028IleAla: 6.028 ± 0.587
0.157IleCys: 0.157 ± 0.08
3.25IleAsp: 3.25 ± 0.38
3.145IleGlu: 3.145 ± 0.446
0.419IlePhe: 0.419 ± 0.117
4.193IleGly: 4.193 ± 0.744
0.367IleHis: 0.367 ± 0.15
0.629IleIle: 0.629 ± 0.215
1.677IleLys: 1.677 ± 0.314
2.044IleLeu: 2.044 ± 0.33
0.472IleMet: 0.472 ± 0.14
1.363IleAsn: 1.363 ± 0.249
1.834IlePro: 1.834 ± 0.355
0.681IleGln: 0.681 ± 0.204
2.201IleArg: 2.201 ± 0.301
1.625IleSer: 1.625 ± 0.25
2.516IleThr: 2.516 ± 0.341
3.459IleVal: 3.459 ± 0.402
0.524IleTrp: 0.524 ± 0.162
0.367IleTyr: 0.367 ± 0.156
0.0IleXaa: 0.0 ± 0.0
Lys
3.669LysAla: 3.669 ± 0.545
0.367LysCys: 0.367 ± 0.144
1.31LysAsp: 1.31 ± 0.233
0.472LysGlu: 0.472 ± 0.162
0.681LysPhe: 0.681 ± 0.152
2.988LysGly: 2.988 ± 0.54
0.681LysHis: 0.681 ± 0.188
1.468LysIle: 1.468 ± 0.299
0.734LysLys: 0.734 ± 0.18
3.197LysLeu: 3.197 ± 0.338
0.577LysMet: 0.577 ± 0.177
0.577LysAsn: 0.577 ± 0.184
3.197LysPro: 3.197 ± 0.428
0.629LysGln: 0.629 ± 0.174
2.621LysArg: 2.621 ± 0.348
1.101LysSer: 1.101 ± 0.21
2.359LysThr: 2.359 ± 0.354
2.516LysVal: 2.516 ± 0.36
0.629LysTrp: 0.629 ± 0.199
0.996LysTyr: 0.996 ± 0.32
0.0LysXaa: 0.0 ± 0.0
Leu
12.894LeuAla: 12.894 ± 0.794
0.943LeuCys: 0.943 ± 0.223
8.491LeuAsp: 8.491 ± 0.697
2.097LeuGlu: 2.097 ± 0.32
2.306LeuPhe: 2.306 ± 0.422
7.338LeuGly: 7.338 ± 0.695
2.097LeuHis: 2.097 ± 0.344
3.459LeuIle: 3.459 ± 0.348
2.254LeuLys: 2.254 ± 0.383
5.556LeuLeu: 5.556 ± 0.603
1.677LeuMet: 1.677 ± 0.247
2.306LeuAsn: 2.306 ± 0.304
3.826LeuPro: 3.826 ± 0.603
2.778LeuGln: 2.778 ± 0.383
6.552LeuArg: 6.552 ± 0.537
5.608LeuSer: 5.608 ± 0.454
4.979LeuThr: 4.979 ± 0.519
5.975LeuVal: 5.975 ± 0.503
1.572LeuTrp: 1.572 ± 0.266
1.572LeuTyr: 1.572 ± 0.303
0.0LeuXaa: 0.0 ± 0.0
Met
2.935MetAla: 2.935 ± 0.32
0.157MetCys: 0.157 ± 0.091
0.786MetAsp: 0.786 ± 0.149
0.734MetGlu: 0.734 ± 0.214
0.786MetPhe: 0.786 ± 0.188
1.468MetGly: 1.468 ± 0.262
0.577MetHis: 0.577 ± 0.182
1.363MetIle: 1.363 ± 0.269
0.262MetLys: 0.262 ± 0.152
1.73MetLeu: 1.73 ± 0.271
0.314MetMet: 0.314 ± 0.116
0.419MetAsn: 0.419 ± 0.146
1.415MetPro: 1.415 ± 0.25
0.577MetGln: 0.577 ± 0.185
1.677MetArg: 1.677 ± 0.295
2.568MetSer: 2.568 ± 0.315
1.363MetThr: 1.363 ± 0.251
1.73MetVal: 1.73 ± 0.281
0.524MetTrp: 0.524 ± 0.154
0.524MetTyr: 0.524 ± 0.159
0.0MetXaa: 0.0 ± 0.0
Asn
3.826AsnAla: 3.826 ± 0.503
0.262AsnCys: 0.262 ± 0.118
1.048AsnAsp: 1.048 ± 0.277
0.839AsnGlu: 0.839 ± 0.203
0.786AsnPhe: 0.786 ± 0.228
3.25AsnGly: 3.25 ± 0.437
0.367AsnHis: 0.367 ± 0.131
0.629AsnIle: 0.629 ± 0.226
0.524AsnLys: 0.524 ± 0.184
1.73AsnLeu: 1.73 ± 0.324
0.262AsnMet: 0.262 ± 0.109
0.629AsnAsn: 0.629 ± 0.224
2.463AsnPro: 2.463 ± 0.345
0.786AsnGln: 0.786 ± 0.193
1.939AsnArg: 1.939 ± 0.285
0.786AsnSer: 0.786 ± 0.191
1.625AsnThr: 1.625 ± 0.26
2.516AsnVal: 2.516 ± 0.387
0.367AsnTrp: 0.367 ± 0.102
0.996AsnTyr: 0.996 ± 0.25
0.0AsnXaa: 0.0 ± 0.0
Pro
8.334ProAla: 8.334 ± 0.747
0.419ProCys: 0.419 ± 0.169
3.774ProAsp: 3.774 ± 0.474
4.508ProGlu: 4.508 ± 0.584
1.625ProPhe: 1.625 ± 0.288
6.08ProGly: 6.08 ± 0.619
1.153ProHis: 1.153 ± 0.262
1.992ProIle: 1.992 ± 0.353
1.73ProLys: 1.73 ± 0.277
4.298ProLeu: 4.298 ± 0.456
0.996ProMet: 0.996 ± 0.184
1.153ProAsn: 1.153 ± 0.371
2.83ProPro: 2.83 ± 0.411
1.52ProGln: 1.52 ± 0.357
3.354ProArg: 3.354 ± 0.472
2.726ProSer: 2.726 ± 0.412
3.092ProThr: 3.092 ± 0.511
4.822ProVal: 4.822 ± 0.57
1.048ProTrp: 1.048 ± 0.217
1.363ProTyr: 1.363 ± 0.242
0.0ProXaa: 0.0 ± 0.0
Gln
4.298GlnAla: 4.298 ± 0.545
0.262GlnCys: 0.262 ± 0.12
0.943GlnAsp: 0.943 ± 0.195
0.786GlnGlu: 0.786 ± 0.195
0.734GlnPhe: 0.734 ± 0.343
2.359GlnGly: 2.359 ± 0.294
0.891GlnHis: 0.891 ± 0.186
1.677GlnIle: 1.677 ± 0.25
0.367GlnLys: 0.367 ± 0.133
2.201GlnLeu: 2.201 ± 0.306
0.786GlnMet: 0.786 ± 0.189
0.419GlnAsn: 0.419 ± 0.167
1.887GlnPro: 1.887 ± 0.273
1.52GlnGln: 1.52 ± 0.243
2.306GlnArg: 2.306 ± 0.307
1.782GlnSer: 1.782 ± 0.303
1.782GlnThr: 1.782 ± 0.255
3.04GlnVal: 3.04 ± 0.413
0.734GlnTrp: 0.734 ± 0.228
1.101GlnTyr: 1.101 ± 0.236
0.0GlnXaa: 0.0 ± 0.0
Arg
7.443ArgAla: 7.443 ± 0.722
1.363ArgCys: 1.363 ± 0.303
4.193ArgAsp: 4.193 ± 0.564
4.403ArgGlu: 4.403 ± 0.546
1.52ArgPhe: 1.52 ± 0.283
4.036ArgGly: 4.036 ± 0.487
1.887ArgHis: 1.887 ± 0.381
2.568ArgIle: 2.568 ± 0.439
3.092ArgLys: 3.092 ± 0.426
6.447ArgLeu: 6.447 ± 0.611
2.411ArgMet: 2.411 ± 0.349
2.254ArgAsn: 2.254 ± 0.352
3.617ArgPro: 3.617 ± 0.52
2.516ArgGln: 2.516 ± 0.318
6.657ArgArg: 6.657 ± 0.935
3.25ArgSer: 3.25 ± 0.335
4.036ArgThr: 4.036 ± 0.466
4.298ArgVal: 4.298 ± 0.486
1.887ArgTrp: 1.887 ± 0.347
1.992ArgTyr: 1.992 ± 0.275
0.0ArgXaa: 0.0 ± 0.0
Ser
6.866SerAla: 6.866 ± 0.606
0.524SerCys: 0.524 ± 0.199
2.411SerAsp: 2.411 ± 0.309
2.883SerGlu: 2.883 ± 0.385
1.73SerPhe: 1.73 ± 0.295
4.088SerGly: 4.088 ± 0.72
1.468SerHis: 1.468 ± 0.327
2.463SerIle: 2.463 ± 0.42
1.153SerLys: 1.153 ± 0.197
4.77SerLeu: 4.77 ± 0.397
1.52SerMet: 1.52 ± 0.268
1.572SerAsn: 1.572 ± 0.259
3.721SerPro: 3.721 ± 0.412
1.625SerGln: 1.625 ± 0.313
3.354SerArg: 3.354 ± 0.298
2.83SerSer: 2.83 ± 0.507
2.621SerThr: 2.621 ± 0.291
3.197SerVal: 3.197 ± 0.452
1.206SerTrp: 1.206 ± 0.257
1.415SerTyr: 1.415 ± 0.194
0.0SerXaa: 0.0 ± 0.0
Thr
6.814ThrAla: 6.814 ± 0.804
0.943ThrCys: 0.943 ± 0.203
3.354ThrAsp: 3.354 ± 0.408
3.826ThrGlu: 3.826 ± 0.352
2.149ThrPhe: 2.149 ± 0.317
6.08ThrGly: 6.08 ± 0.558
1.415ThrHis: 1.415 ± 0.293
3.04ThrIle: 3.04 ± 0.398
1.834ThrLys: 1.834 ± 0.294
4.56ThrLeu: 4.56 ± 0.474
0.786ThrMet: 0.786 ± 0.198
1.415ThrAsn: 1.415 ± 0.234
3.931ThrPro: 3.931 ± 0.507
1.52ThrGln: 1.52 ± 0.224
2.726ThrArg: 2.726 ± 0.431
2.726ThrSer: 2.726 ± 0.397
3.774ThrThr: 3.774 ± 0.411
5.399ThrVal: 5.399 ± 0.434
0.891ThrTrp: 0.891 ± 0.245
1.992ThrTyr: 1.992 ± 0.357
0.0ThrXaa: 0.0 ± 0.0
Val
10.745ValAla: 10.745 ± 0.859
1.153ValCys: 1.153 ± 0.231
5.084ValAsp: 5.084 ± 0.483
5.451ValGlu: 5.451 ± 0.749
1.939ValPhe: 1.939 ± 0.297
6.761ValGly: 6.761 ± 0.635
2.149ValHis: 2.149 ± 0.44
2.935ValIle: 2.935 ± 0.411
2.778ValLys: 2.778 ± 0.397
6.499ValLeu: 6.499 ± 0.515
1.415ValMet: 1.415 ± 0.241
1.939ValAsn: 1.939 ± 0.265
5.399ValPro: 5.399 ± 0.52
1.992ValGln: 1.992 ± 0.316
4.403ValArg: 4.403 ± 0.488
3.879ValSer: 3.879 ± 0.387
5.241ValThr: 5.241 ± 0.435
5.766ValVal: 5.766 ± 0.721
1.782ValTrp: 1.782 ± 0.304
1.73ValTyr: 1.73 ± 0.317
0.0ValXaa: 0.0 ± 0.0
Trp
2.463TrpAla: 2.463 ± 0.435
0.314TrpCys: 0.314 ± 0.124
0.943TrpAsp: 0.943 ± 0.219
0.681TrpGlu: 0.681 ± 0.233
0.629TrpPhe: 0.629 ± 0.137
1.153TrpGly: 1.153 ± 0.269
0.734TrpHis: 0.734 ± 0.19
1.048TrpIle: 1.048 ± 0.234
0.157TrpLys: 0.157 ± 0.082
2.83TrpLeu: 2.83 ± 0.369
0.367TrpMet: 0.367 ± 0.142
0.577TrpAsn: 0.577 ± 0.178
0.891TrpPro: 0.891 ± 0.215
1.153TrpGln: 1.153 ± 0.22
1.887TrpArg: 1.887 ± 0.335
0.996TrpSer: 0.996 ± 0.22
1.206TrpThr: 1.206 ± 0.246
1.153TrpVal: 1.153 ± 0.291
0.367TrpTrp: 0.367 ± 0.126
0.419TrpTyr: 0.419 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.83TyrAla: 2.83 ± 0.377
0.314TyrCys: 0.314 ± 0.109
1.887TyrAsp: 1.887 ± 0.292
1.52TyrGlu: 1.52 ± 0.27
0.472TyrPhe: 0.472 ± 0.168
2.411TyrGly: 2.411 ± 0.287
0.21TyrHis: 0.21 ± 0.114
0.314TyrIle: 0.314 ± 0.15
0.577TyrLys: 0.577 ± 0.194
2.883TyrLeu: 2.883 ± 0.422
0.367TyrMet: 0.367 ± 0.121
0.786TyrAsn: 0.786 ± 0.18
1.52TyrPro: 1.52 ± 0.271
0.367TyrGln: 0.367 ± 0.171
2.044TyrArg: 2.044 ± 0.312
1.572TyrSer: 1.572 ± 0.283
1.677TyrThr: 1.677 ± 0.297
2.726TyrVal: 2.726 ± 0.402
0.419TyrTrp: 0.419 ± 0.136
0.524TyrTyr: 0.524 ± 0.179
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (19080 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski